Experiment: Cobalt chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS21265 and BWI76_RS21270 are separated by 53 nucleotides BWI76_RS21270 and BWI76_RS21275 overlap by 11 nucleotides BWI76_RS21275 and BWI76_RS21280 overlap by 4 nucleotides BWI76_RS21280 and BWI76_RS21285 are separated by 19 nucleotides
BWI76_RS21265: BWI76_RS21265 - phosphoribosylformylglycinamidine synthase, at 4,452,929 to 4,456,816
_RS21265
BWI76_RS21270: BWI76_RS21270 - phosphoribosylformyl-glycineamide synthetase, at 4,456,870 to 4,457,001
_RS21270
BWI76_RS21275: BWI76_RS21275 - lytic transglycosylase F, at 4,456,991 to 4,458,607
_RS21275
BWI76_RS21280: BWI76_RS21280 - tRNA adenosine(34) deaminase TadA, at 4,458,604 to 4,459,149
_RS21280
BWI76_RS21285: BWI76_RS21285 - HAD family hydrolase, at 4,459,169 to 4,459,804
_RS21285
Position (kb)
4456
4457
4458
4459 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 4455.995 kb on - strand, within BWI76_RS21265 at 4456.010 kb on + strand, within BWI76_RS21265 at 4456.176 kb on + strand, within BWI76_RS21265 at 4456.509 kb on + strand at 4456.517 kb on - strand at 4456.534 kb on - strand at 4456.609 kb on + strand at 4456.609 kb on + strand at 4456.737 kb on - strand at 4456.875 kb on - strand at 4457.009 kb on - strand at 4457.480 kb on + strand, within BWI76_RS21275 at 4457.524 kb on + strand, within BWI76_RS21275 at 4457.617 kb on - strand, within BWI76_RS21275 at 4457.684 kb on + strand, within BWI76_RS21275 at 4457.738 kb on - strand, within BWI76_RS21275 at 4458.374 kb on + strand, within BWI76_RS21275 at 4458.380 kb on + strand, within BWI76_RS21275 at 4458.382 kb on - strand, within BWI76_RS21275 at 4458.492 kb on - strand at 4458.607 kb on + strand at 4459.171 kb on + strand at 4459.398 kb on + strand, within BWI76_RS21285
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.5 mM remove 4,455,995 - BWI76_RS21265 0.79 -0.2 4,456,010 + BWI76_RS21265 0.79 -1.9 4,456,176 + BWI76_RS21265 0.84 +0.1 4,456,509 + +0.5 4,456,517 - +0.3 4,456,534 - -2.6 4,456,609 + -0.2 4,456,609 + +0.3 4,456,737 - -0.8 4,456,875 - -0.4 4,457,009 - -0.8 4,457,480 + BWI76_RS21275 0.30 -3.8 4,457,524 + BWI76_RS21275 0.33 +0.4 4,457,617 - BWI76_RS21275 0.39 -3.3 4,457,684 + BWI76_RS21275 0.43 -2.2 4,457,738 - BWI76_RS21275 0.46 -2.3 4,458,374 + BWI76_RS21275 0.86 -3.8 4,458,380 + BWI76_RS21275 0.86 -0.4 4,458,382 - BWI76_RS21275 0.86 -2.5 4,458,492 - -0.6 4,458,607 + +4.5 4,459,171 + -2.3 4,459,398 + BWI76_RS21285 0.36 -1.2
Or see this region's nucleotide sequence