Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS21275

Experiment: Cobalt chloride 0.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS21265 and BWI76_RS21270 are separated by 53 nucleotidesBWI76_RS21270 and BWI76_RS21275 overlap by 11 nucleotidesBWI76_RS21275 and BWI76_RS21280 overlap by 4 nucleotidesBWI76_RS21280 and BWI76_RS21285 are separated by 19 nucleotides BWI76_RS21265: BWI76_RS21265 - phosphoribosylformylglycinamidine synthase, at 4,452,929 to 4,456,816 _RS21265 BWI76_RS21270: BWI76_RS21270 - phosphoribosylformyl-glycineamide synthetase, at 4,456,870 to 4,457,001 _RS21270 BWI76_RS21275: BWI76_RS21275 - lytic transglycosylase F, at 4,456,991 to 4,458,607 _RS21275 BWI76_RS21280: BWI76_RS21280 - tRNA adenosine(34) deaminase TadA, at 4,458,604 to 4,459,149 _RS21280 BWI76_RS21285: BWI76_RS21285 - HAD family hydrolase, at 4,459,169 to 4,459,804 _RS21285 Position (kb) 4456 4457 4458 4459Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4at 4455.995 kb on - strand, within BWI76_RS21265at 4456.010 kb on + strand, within BWI76_RS21265at 4456.176 kb on + strand, within BWI76_RS21265at 4456.509 kb on + strandat 4456.517 kb on - strandat 4456.534 kb on - strandat 4456.609 kb on + strandat 4456.609 kb on + strandat 4456.737 kb on - strandat 4456.875 kb on - strandat 4457.009 kb on - strandat 4457.480 kb on + strand, within BWI76_RS21275at 4457.524 kb on + strand, within BWI76_RS21275at 4457.617 kb on - strand, within BWI76_RS21275at 4457.684 kb on + strand, within BWI76_RS21275at 4457.738 kb on - strand, within BWI76_RS21275at 4458.374 kb on + strand, within BWI76_RS21275at 4458.380 kb on + strand, within BWI76_RS21275at 4458.382 kb on - strand, within BWI76_RS21275at 4458.492 kb on - strandat 4458.607 kb on + strandat 4459.171 kb on + strandat 4459.398 kb on + strand, within BWI76_RS21285

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Per-strain Table

Position Strand Gene LocusTag Fraction Cobalt chloride 0.5 mM
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4,455,995 - BWI76_RS21265 0.79 -0.2
4,456,010 + BWI76_RS21265 0.79 -1.9
4,456,176 + BWI76_RS21265 0.84 +0.1
4,456,509 + +0.5
4,456,517 - +0.3
4,456,534 - -2.6
4,456,609 + -0.2
4,456,609 + +0.3
4,456,737 - -0.8
4,456,875 - -0.4
4,457,009 - -0.8
4,457,480 + BWI76_RS21275 0.30 -3.8
4,457,524 + BWI76_RS21275 0.33 +0.4
4,457,617 - BWI76_RS21275 0.39 -3.3
4,457,684 + BWI76_RS21275 0.43 -2.2
4,457,738 - BWI76_RS21275 0.46 -2.3
4,458,374 + BWI76_RS21275 0.86 -3.8
4,458,380 + BWI76_RS21275 0.86 -0.4
4,458,382 - BWI76_RS21275 0.86 -2.5
4,458,492 - -0.6
4,458,607 + +4.5
4,459,171 + -2.3
4,459,398 + BWI76_RS21285 0.36 -1.2

Or see this region's nucleotide sequence