Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS15125

Experiment: Cobalt chloride 0.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS15115 and BWI76_RS15120 are separated by 677 nucleotidesBWI76_RS15120 and BWI76_RS15125 overlap by 7 nucleotidesBWI76_RS15125 and BWI76_RS15130 overlap by 4 nucleotidesBWI76_RS15130 and BWI76_RS15135 are separated by 300 nucleotides BWI76_RS15115: BWI76_RS15115 - MFS transporter, at 3,168,495 to 3,169,727 _RS15115 BWI76_RS15120: BWI76_RS15120 - hypothetical protein, at 3,170,405 to 3,170,623 _RS15120 BWI76_RS15125: BWI76_RS15125 - acetyltransferase, at 3,170,617 to 3,171,195 _RS15125 BWI76_RS15130: BWI76_RS15130 - hypothetical protein, at 3,171,192 to 3,171,638 _RS15130 BWI76_RS15135: BWI76_RS15135 - hypothetical protein, at 3,171,939 to 3,172,229 _RS15135 Position (kb) 3170 3171 3172Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 3169.629 kb on - strandat 3169.629 kb on - strandat 3169.629 kb on - strandat 3169.629 kb on - strandat 3169.629 kb on - strandat 3169.629 kb on - strandat 3169.629 kb on - strandat 3169.640 kb on - strandat 3169.640 kb on - strandat 3169.640 kb on - strandat 3169.678 kb on + strandat 3169.684 kb on - strandat 3169.686 kb on - strandat 3169.686 kb on - strandat 3169.741 kb on - strandat 3169.741 kb on - strandat 3169.977 kb on - strandat 3170.019 kb on + strandat 3170.019 kb on + strandat 3170.019 kb on + strandat 3170.019 kb on + strandat 3170.019 kb on + strandat 3170.019 kb on + strandat 3170.019 kb on + strandat 3170.019 kb on - strandat 3170.026 kb on + strandat 3170.026 kb on + strandat 3170.031 kb on + strandat 3170.031 kb on + strandat 3170.034 kb on - strandat 3170.034 kb on - strandat 3170.039 kb on - strandat 3170.051 kb on - strandat 3170.213 kb on + strandat 3170.300 kb on + strandat 3170.346 kb on + strandat 3170.402 kb on + strandat 3170.402 kb on + strandat 3170.402 kb on + strandat 3170.410 kb on - strandat 3170.410 kb on - strandat 3170.410 kb on - strandat 3170.410 kb on - strandat 3170.410 kb on - strandat 3170.418 kb on - strandat 3170.422 kb on + strandat 3170.422 kb on + strandat 3170.423 kb on - strandat 3170.430 kb on - strand, within BWI76_RS15120at 3170.439 kb on - strand, within BWI76_RS15120at 3170.445 kb on - strandat 3170.496 kb on - strand, within BWI76_RS15120at 3170.511 kb on + strand, within BWI76_RS15120at 3170.519 kb on - strandat 3170.548 kb on - strand, within BWI76_RS15120at 3170.625 kb on + strandat 3170.633 kb on - strandat 3170.655 kb on - strandat 3170.707 kb on - strand, within BWI76_RS15125at 3170.814 kb on - strand, within BWI76_RS15125at 3170.822 kb on - strand, within BWI76_RS15125at 3170.880 kb on + strand, within BWI76_RS15125at 3170.991 kb on + strand, within BWI76_RS15125at 3171.183 kb on - strandat 3171.359 kb on + strand, within BWI76_RS15130at 3171.413 kb on - strand, within BWI76_RS15130at 3171.745 kb on - strandat 3171.904 kb on - strandat 3172.060 kb on - strand, within BWI76_RS15135at 3172.090 kb on - strand, within BWI76_RS15135at 3172.159 kb on + strand, within BWI76_RS15135at 3172.167 kb on - strand, within BWI76_RS15135

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Per-strain Table

Position Strand Gene LocusTag Fraction Cobalt chloride 0.5 mM
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3,169,629 - -0.2
3,169,629 - +0.8
3,169,629 - +1.6
3,169,629 - -0.7
3,169,629 - -0.4
3,169,629 - -1.6
3,169,629 - -0.1
3,169,640 - -1.4
3,169,640 - -1.2
3,169,640 - -1.6
3,169,678 + +0.2
3,169,684 - -1.4
3,169,686 - +0.3
3,169,686 - +0.7
3,169,741 - +1.2
3,169,741 - +0.8
3,169,977 - -1.1
3,170,019 + +1.3
3,170,019 + -1.9
3,170,019 + -0.5
3,170,019 + -0.4
3,170,019 + +1.2
3,170,019 + -2.6
3,170,019 + -0.4
3,170,019 - -0.0
3,170,026 + +1.1
3,170,026 + +1.4
3,170,031 + +0.8
3,170,031 + +0.4
3,170,034 - -0.6
3,170,034 - -2.1
3,170,039 - -1.7
3,170,051 - +0.8
3,170,213 + +1.8
3,170,300 + +0.3
3,170,346 + +0.2
3,170,402 + -1.6
3,170,402 + -1.9
3,170,402 + -1.1
3,170,410 - +0.9
3,170,410 - -0.8
3,170,410 - -0.2
3,170,410 - -0.8
3,170,410 - -1.8
3,170,418 - -0.6
3,170,422 + +1.2
3,170,422 + -0.4
3,170,423 - +1.0
3,170,430 - BWI76_RS15120 0.11 +0.6
3,170,439 - BWI76_RS15120 0.16 +1.5
3,170,445 - +2.8
3,170,496 - BWI76_RS15120 0.42 -0.7
3,170,511 + BWI76_RS15120 0.48 +3.2
3,170,519 - +4.4
3,170,548 - BWI76_RS15120 0.65 -1.0
3,170,625 + -1.0
3,170,633 - -0.5
3,170,655 - +1.0
3,170,707 - BWI76_RS15125 0.16 +0.1
3,170,814 - BWI76_RS15125 0.34 -0.5
3,170,822 - BWI76_RS15125 0.35 +0.8
3,170,880 + BWI76_RS15125 0.45 +0.7
3,170,991 + BWI76_RS15125 0.65 +1.6
3,171,183 - -1.5
3,171,359 + BWI76_RS15130 0.37 -0.8
3,171,413 - BWI76_RS15130 0.49 +0.3
3,171,745 - -1.3
3,171,904 - -1.0
3,172,060 - BWI76_RS15135 0.42 +1.0
3,172,090 - BWI76_RS15135 0.52 +1.8
3,172,159 + BWI76_RS15135 0.76 -0.0
3,172,167 - BWI76_RS15135 0.78 +0.4

Or see this region's nucleotide sequence