Experiment: Cobalt chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13570 and BWI76_RS13575 are separated by 304 nucleotides BWI76_RS13575 and BWI76_RS13580 are separated by 100 nucleotides BWI76_RS13580 and BWI76_RS13585 are separated by 68 nucleotides
BWI76_RS13570: BWI76_RS13570 - mandelate racemase/muconate lactonizing protein, at 2,829,188 to 2,830,384
_RS13570
BWI76_RS13575: BWI76_RS13575 - sugar binding transcriptional regulator, LacI family protein, at 2,830,689 to 2,831,693
_RS13575
BWI76_RS13580: BWI76_RS13580 - glutathione S-transferase domain protein, at 2,831,794 to 2,832,411
_RS13580
BWI76_RS13585: BWI76_RS13585 - 4-oxalocrotonate tautomerase, at 2,832,480 to 2,832,707
_RS13585
Position (kb)
2830
2831
2832 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2829.695 kb on + strand, within BWI76_RS13570 at 2829.800 kb on - strand, within BWI76_RS13570 at 2829.906 kb on - strand, within BWI76_RS13570 at 2829.995 kb on - strand, within BWI76_RS13570 at 2829.997 kb on + strand, within BWI76_RS13570 at 2830.087 kb on + strand, within BWI76_RS13570 at 2830.957 kb on + strand, within BWI76_RS13575 at 2831.040 kb on + strand, within BWI76_RS13575 at 2831.250 kb on + strand, within BWI76_RS13575 at 2831.250 kb on + strand, within BWI76_RS13575 at 2831.302 kb on + strand, within BWI76_RS13575 at 2831.722 kb on + strand at 2831.739 kb on + strand at 2832.196 kb on - strand, within BWI76_RS13580 at 2832.609 kb on - strand, within BWI76_RS13585 at 2832.621 kb on + strand, within BWI76_RS13585 at 2832.631 kb on + strand, within BWI76_RS13585
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.5 mM remove 2,829,695 + BWI76_RS13570 0.42 +0.3 2,829,800 - BWI76_RS13570 0.51 +1.1 2,829,906 - BWI76_RS13570 0.60 -2.0 2,829,995 - BWI76_RS13570 0.67 +1.0 2,829,997 + BWI76_RS13570 0.68 +0.4 2,830,087 + BWI76_RS13570 0.75 +0.0 2,830,957 + BWI76_RS13575 0.27 -3.4 2,831,040 + BWI76_RS13575 0.35 -2.4 2,831,250 + BWI76_RS13575 0.56 -1.3 2,831,250 + BWI76_RS13575 0.56 -1.2 2,831,302 + BWI76_RS13575 0.61 -1.8 2,831,722 + +0.7 2,831,739 + +0.1 2,832,196 - BWI76_RS13580 0.65 +1.5 2,832,609 - BWI76_RS13585 0.57 +0.8 2,832,621 + BWI76_RS13585 0.62 +0.3 2,832,631 + BWI76_RS13585 0.66 +0.3
Or see this region's nucleotide sequence