Experiment: Cobalt chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13260 and BWI76_RS13265 are separated by 51 nucleotides BWI76_RS13265 and BWI76_RS13270 are separated by 173 nucleotides BWI76_RS13270 and BWI76_RS13275 are separated by 38 nucleotides BWI76_RS13275 and BWI76_RS13280 are separated by 68 nucleotides
BWI76_RS13260: BWI76_RS13260 - S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase, at 2,764,618 to 2,765,736
_RS13260
BWI76_RS13265: BWI76_RS13265 - hypothetical protein, at 2,765,788 to 2,766,066
_RS13265
BWI76_RS13270: BWI76_RS13270 - membrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family, at 2,766,240 to 2,768,612
_RS13270
BWI76_RS13275: BWI76_RS13275 - hypothetical protein, at 2,768,651 to 2,768,836
_RS13275
BWI76_RS13280: BWI76_RS13280 - LysR family transcriptional regulator, at 2,768,905 to 2,769,828
_RS13280
Position (kb)
2766
2767
2768
2769 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2765.249 kb on - strand, within BWI76_RS13260 at 2765.256 kb on - strand, within BWI76_RS13260 at 2765.340 kb on + strand, within BWI76_RS13260 at 2765.572 kb on + strand, within BWI76_RS13260 at 2765.654 kb on - strand at 2765.840 kb on - strand, within BWI76_RS13265 at 2765.883 kb on - strand, within BWI76_RS13265 at 2765.883 kb on - strand, within BWI76_RS13265 at 2765.998 kb on + strand, within BWI76_RS13265 at 2767.015 kb on + strand, within BWI76_RS13270 at 2767.023 kb on - strand, within BWI76_RS13270 at 2767.133 kb on + strand, within BWI76_RS13270 at 2767.297 kb on + strand, within BWI76_RS13270 at 2767.655 kb on + strand, within BWI76_RS13270 at 2767.984 kb on - strand, within BWI76_RS13270 at 2768.034 kb on - strand, within BWI76_RS13270 at 2768.044 kb on - strand, within BWI76_RS13270 at 2768.068 kb on - strand, within BWI76_RS13270 at 2768.281 kb on + strand, within BWI76_RS13270 at 2768.573 kb on - strand at 2768.763 kb on + strand, within BWI76_RS13275 at 2769.007 kb on - strand, within BWI76_RS13280 at 2769.080 kb on - strand, within BWI76_RS13280 at 2769.505 kb on - strand, within BWI76_RS13280 at 2769.507 kb on - strand, within BWI76_RS13280 at 2769.574 kb on - strand, within BWI76_RS13280 at 2769.577 kb on - strand, within BWI76_RS13280
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.5 mM remove 2,765,249 - BWI76_RS13260 0.56 -0.9 2,765,256 - BWI76_RS13260 0.57 -1.9 2,765,340 + BWI76_RS13260 0.65 +0.4 2,765,572 + BWI76_RS13260 0.85 +1.6 2,765,654 - +1.0 2,765,840 - BWI76_RS13265 0.19 +2.5 2,765,883 - BWI76_RS13265 0.34 -0.5 2,765,883 - BWI76_RS13265 0.34 +0.6 2,765,998 + BWI76_RS13265 0.75 +1.2 2,767,015 + BWI76_RS13270 0.33 +0.2 2,767,023 - BWI76_RS13270 0.33 -0.5 2,767,133 + BWI76_RS13270 0.38 -0.3 2,767,297 + BWI76_RS13270 0.45 -1.4 2,767,655 + BWI76_RS13270 0.60 -0.1 2,767,984 - BWI76_RS13270 0.73 +0.0 2,768,034 - BWI76_RS13270 0.76 -0.2 2,768,044 - BWI76_RS13270 0.76 -0.1 2,768,068 - BWI76_RS13270 0.77 +1.6 2,768,281 + BWI76_RS13270 0.86 +1.6 2,768,573 - +1.1 2,768,763 + BWI76_RS13275 0.60 +0.1 2,769,007 - BWI76_RS13280 0.11 -0.4 2,769,080 - BWI76_RS13280 0.19 +0.8 2,769,505 - BWI76_RS13280 0.65 -0.2 2,769,507 - BWI76_RS13280 0.65 +0.2 2,769,574 - BWI76_RS13280 0.72 -0.6 2,769,577 - BWI76_RS13280 0.73 +0.1
Or see this region's nucleotide sequence