Experiment: Cobalt chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12225 and BWI76_RS12230 are separated by 481 nucleotides BWI76_RS12230 and BWI76_RS12235 are separated by 244 nucleotides BWI76_RS12235 and BWI76_RS12240 are separated by 3 nucleotides
BWI76_RS12225: BWI76_RS12225 - hypothetical protein, at 2,556,370 to 2,556,543
_RS12225
BWI76_RS12230: BWI76_RS12230 - hypothetical protein, at 2,557,025 to 2,557,270
_RS12230
BWI76_RS12235: BWI76_RS12235 - hypothetical protein, at 2,557,515 to 2,557,703
_RS12235
BWI76_RS12240: BWI76_RS12240 - terminase small subunit, at 2,557,707 to 2,558,699
_RS12240
Position (kb)
2557
2558 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6
7
8
9 at 2556.576 kb on + strand at 2556.576 kb on + strand at 2556.579 kb on + strand at 2556.579 kb on + strand at 2556.589 kb on + strand at 2556.589 kb on + strand at 2556.591 kb on - strand at 2556.591 kb on - strand at 2556.592 kb on - strand at 2556.592 kb on - strand at 2556.601 kb on - strand at 2556.621 kb on - strand at 2556.636 kb on + strand at 2556.636 kb on + strand at 2556.658 kb on - strand at 2556.662 kb on + strand at 2556.663 kb on - strand at 2556.686 kb on - strand at 2556.767 kb on + strand at 2556.806 kb on + strand at 2556.808 kb on + strand at 2556.808 kb on + strand at 2556.808 kb on + strand at 2556.808 kb on + strand at 2556.816 kb on - strand at 2556.816 kb on - strand at 2556.840 kb on + strand at 2556.841 kb on + strand at 2556.842 kb on + strand at 2556.843 kb on + strand at 2556.843 kb on - strand at 2556.848 kb on - strand at 2556.867 kb on - strand at 2556.867 kb on - strand at 2556.868 kb on - strand at 2556.870 kb on + strand at 2556.870 kb on + strand at 2556.870 kb on + strand at 2556.870 kb on + strand at 2556.870 kb on + strand at 2556.870 kb on + strand at 2556.878 kb on - strand at 2556.878 kb on - strand at 2556.878 kb on - strand at 2556.878 kb on - strand at 2556.878 kb on - strand at 2556.879 kb on - strand at 2556.886 kb on - strand at 2556.889 kb on - strand at 2556.900 kb on - strand at 2556.907 kb on + strand at 2557.064 kb on - strand, within BWI76_RS12230 at 2557.081 kb on + strand, within BWI76_RS12230 at 2557.118 kb on + strand, within BWI76_RS12230 at 2557.118 kb on + strand, within BWI76_RS12230 at 2557.123 kb on + strand, within BWI76_RS12230 at 2557.126 kb on - strand, within BWI76_RS12230 at 2557.126 kb on - strand, within BWI76_RS12230 at 2557.208 kb on - strand, within BWI76_RS12230 at 2557.211 kb on + strand, within BWI76_RS12230 at 2557.216 kb on + strand, within BWI76_RS12230 at 2557.219 kb on - strand, within BWI76_RS12230 at 2557.219 kb on - strand, within BWI76_RS12230 at 2557.236 kb on - strand, within BWI76_RS12230 at 2557.248 kb on - strand at 2557.256 kb on + strand at 2557.256 kb on + strand at 2557.256 kb on + strand at 2557.291 kb on - strand at 2557.331 kb on + strand at 2557.331 kb on + strand at 2557.331 kb on + strand at 2557.339 kb on - strand at 2557.346 kb on + strand at 2557.346 kb on + strand at 2557.346 kb on + strand at 2557.346 kb on + strand at 2557.354 kb on - strand at 2557.354 kb on - strand at 2557.354 kb on - strand at 2557.436 kb on + strand at 2557.454 kb on + strand at 2557.454 kb on + strand at 2557.462 kb on - strand at 2557.462 kb on - strand at 2557.462 kb on - strand at 2557.504 kb on + strand at 2557.592 kb on - strand, within BWI76_RS12235 at 2557.607 kb on + strand, within BWI76_RS12235 at 2557.698 kb on + strand at 2558.012 kb on - strand, within BWI76_RS12240 at 2558.021 kb on - strand, within BWI76_RS12240 at 2558.146 kb on + strand, within BWI76_RS12240 at 2558.232 kb on + strand, within BWI76_RS12240 at 2558.335 kb on - strand, within BWI76_RS12240 at 2558.356 kb on - strand, within BWI76_RS12240 at 2558.412 kb on - strand, within BWI76_RS12240 at 2558.594 kb on + strand, within BWI76_RS12240 at 2558.687 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.5 mM remove 2,556,576 + +0.8 2,556,576 + +0.4 2,556,579 + -0.6 2,556,579 + +1.0 2,556,589 + -0.9 2,556,589 + +0.2 2,556,591 - +0.7 2,556,591 - +9.4 2,556,592 - +0.4 2,556,592 - +1.0 2,556,601 - -0.1 2,556,621 - +1.7 2,556,636 + -0.5 2,556,636 + -3.1 2,556,658 - +0.7 2,556,662 + -0.5 2,556,663 - -0.1 2,556,686 - -0.3 2,556,767 + +1.7 2,556,806 + -1.2 2,556,808 + +0.4 2,556,808 + -1.1 2,556,808 + -1.0 2,556,808 + -0.4 2,556,816 - +0.7 2,556,816 - -2.9 2,556,840 + +1.1 2,556,841 + -0.0 2,556,842 + -1.5 2,556,843 + +0.3 2,556,843 - -0.4 2,556,848 - +0.9 2,556,867 - -0.8 2,556,867 - +1.1 2,556,868 - -2.2 2,556,870 + +0.6 2,556,870 + +2.7 2,556,870 + -0.0 2,556,870 + -1.8 2,556,870 + -1.6 2,556,870 + -0.9 2,556,878 - -0.3 2,556,878 - -0.6 2,556,878 - +0.4 2,556,878 - -0.2 2,556,878 - -0.5 2,556,879 - -2.0 2,556,886 - +0.5 2,556,889 - -2.2 2,556,900 - -2.5 2,556,907 + -0.9 2,557,064 - BWI76_RS12230 0.16 +2.1 2,557,081 + BWI76_RS12230 0.23 -2.4 2,557,118 + BWI76_RS12230 0.38 -1.2 2,557,118 + BWI76_RS12230 0.38 -0.9 2,557,123 + BWI76_RS12230 0.40 -2.1 2,557,126 - BWI76_RS12230 0.41 -0.8 2,557,126 - BWI76_RS12230 0.41 -2.5 2,557,208 - BWI76_RS12230 0.74 +0.8 2,557,211 + BWI76_RS12230 0.76 -1.3 2,557,216 + BWI76_RS12230 0.78 -0.4 2,557,219 - BWI76_RS12230 0.79 -0.5 2,557,219 - BWI76_RS12230 0.79 -2.0 2,557,236 - BWI76_RS12230 0.86 +1.4 2,557,248 - +0.5 2,557,256 + -1.3 2,557,256 + +0.0 2,557,256 + -2.8 2,557,291 - -2.0 2,557,331 + -0.8 2,557,331 + +0.7 2,557,331 + -0.6 2,557,339 - -1.0 2,557,346 + +0.4 2,557,346 + +0.3 2,557,346 + +0.5 2,557,346 + +0.3 2,557,354 - +0.8 2,557,354 - +0.4 2,557,354 - -2.5 2,557,436 + +0.4 2,557,454 + -0.0 2,557,454 + -1.1 2,557,462 - +0.6 2,557,462 - -0.1 2,557,462 - -0.1 2,557,504 + +0.4 2,557,592 - BWI76_RS12235 0.41 -1.2 2,557,607 + BWI76_RS12235 0.49 +0.1 2,557,698 + +2.0 2,558,012 - BWI76_RS12240 0.31 -2.7 2,558,021 - BWI76_RS12240 0.32 +0.1 2,558,146 + BWI76_RS12240 0.44 +1.7 2,558,232 + BWI76_RS12240 0.53 +0.4 2,558,335 - BWI76_RS12240 0.63 +1.6 2,558,356 - BWI76_RS12240 0.65 +0.0 2,558,412 - BWI76_RS12240 0.71 -2.2 2,558,594 + BWI76_RS12240 0.89 -2.0 2,558,687 + -0.7
Or see this region's nucleotide sequence