Experiment: Cobalt chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08590 and BWI76_RS08595 are separated by 118 nucleotides BWI76_RS08595 and BWI76_RS08600 are separated by 315 nucleotides BWI76_RS08600 and BWI76_RS08605 are separated by 68 nucleotides
BWI76_RS08590: BWI76_RS08590 - zinc transporter ZitB, at 1,812,132 to 1,813,076
_RS08590
BWI76_RS08595: BWI76_RS08595 - hypothetical protein, at 1,813,195 to 1,813,566
_RS08595
BWI76_RS08600: BWI76_RS08600 - 3-deoxy-7-phosphoheptulonate synthase, at 1,813,882 to 1,814,934
_RS08600
BWI76_RS08605: BWI76_RS08605 - phosphoglyceromutase, at 1,815,003 to 1,815,755
_RS08605
Position (kb)
1813
1814
1815 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1812.988 kb on - strand at 1813.082 kb on + strand at 1813.168 kb on - strand at 1813.170 kb on + strand at 1813.178 kb on - strand at 1813.208 kb on - strand at 1813.326 kb on - strand, within BWI76_RS08595 at 1813.337 kb on + strand, within BWI76_RS08595 at 1813.395 kb on - strand, within BWI76_RS08595 at 1813.422 kb on + strand, within BWI76_RS08595 at 1813.454 kb on + strand, within BWI76_RS08595 at 1813.518 kb on + strand, within BWI76_RS08595 at 1813.524 kb on + strand, within BWI76_RS08595 at 1813.634 kb on - strand at 1813.641 kb on + strand at 1813.641 kb on + strand at 1813.721 kb on - strand at 1814.066 kb on - strand, within BWI76_RS08600 at 1814.104 kb on + strand, within BWI76_RS08600 at 1814.266 kb on - strand, within BWI76_RS08600 at 1814.609 kb on - strand, within BWI76_RS08600 at 1814.653 kb on - strand, within BWI76_RS08600 at 1815.018 kb on - strand at 1815.048 kb on + strand at 1815.204 kb on - strand, within BWI76_RS08605 at 1815.288 kb on - strand, within BWI76_RS08605 at 1815.423 kb on - strand, within BWI76_RS08605 at 1815.890 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.5 mM remove 1,812,988 - +0.3 1,813,082 + -1.4 1,813,168 - -2.2 1,813,170 + -0.7 1,813,178 - -0.1 1,813,208 - +0.4 1,813,326 - BWI76_RS08595 0.35 +0.4 1,813,337 + BWI76_RS08595 0.38 +0.6 1,813,395 - BWI76_RS08595 0.54 -0.4 1,813,422 + BWI76_RS08595 0.61 -4.0 1,813,454 + BWI76_RS08595 0.70 -0.3 1,813,518 + BWI76_RS08595 0.87 -0.5 1,813,524 + BWI76_RS08595 0.88 -1.1 1,813,634 - -0.4 1,813,641 + -2.1 1,813,641 + -0.7 1,813,721 - -1.2 1,814,066 - BWI76_RS08600 0.17 +1.2 1,814,104 + BWI76_RS08600 0.21 +0.6 1,814,266 - BWI76_RS08600 0.36 -2.5 1,814,609 - BWI76_RS08600 0.69 +0.9 1,814,653 - BWI76_RS08600 0.73 +0.4 1,815,018 - -2.2 1,815,048 + +0.7 1,815,204 - BWI76_RS08605 0.27 +0.2 1,815,288 - BWI76_RS08605 0.38 -0.2 1,815,423 - BWI76_RS08605 0.56 +0.9 1,815,890 + -2.3
Or see this region's nucleotide sequence