Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS08135

Experiment: Cobalt chloride 0.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS08130 and BWI76_RS08135 are separated by 143 nucleotidesBWI76_RS08135 and BWI76_RS08140 are separated by 208 nucleotides BWI76_RS08130: BWI76_RS08130 - nucleoside triphosphate hydrolase, at 1,722,940 to 1,723,986 _RS08130 BWI76_RS08135: BWI76_RS08135 - tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB, at 1,724,130 to 1,725,554 _RS08135 BWI76_RS08140: BWI76_RS08140 - 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase, at 1,725,763 to 1,726,938 _RS08140 Position (kb) 1724 1725 1726Strain fitness (log2 ratio) -2 -1 0 1 2at 1723.209 kb on - strand, within BWI76_RS08130at 1723.998 kb on - strandat 1724.647 kb on - strand, within BWI76_RS08135at 1724.956 kb on - strand, within BWI76_RS08135at 1725.425 kb on - strandat 1725.491 kb on + strandat 1725.578 kb on - strandat 1725.662 kb on + strandat 1725.667 kb on - strandat 1725.688 kb on - strandat 1725.693 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Cobalt chloride 0.5 mM
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1,723,209 - BWI76_RS08130 0.26 -2.4
1,723,998 - -0.5
1,724,647 - BWI76_RS08135 0.36 +2.3
1,724,956 - BWI76_RS08135 0.58 +2.1
1,725,425 - +2.0
1,725,491 + -0.2
1,725,578 - -0.9
1,725,662 + +0.5
1,725,667 - -0.9
1,725,688 - +0.8
1,725,693 - -0.1

Or see this region's nucleotide sequence