Experiment: Cobalt chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS07480 and BWI76_RS07485 are separated by 153 nucleotides BWI76_RS07485 and BWI76_RS07490 overlap by 72 nucleotides BWI76_RS07490 and BWI76_RS07495 are separated by 492 nucleotides
BWI76_RS07480: BWI76_RS07480 - TonB-dependent siderophore receptor, at 1,577,213 to 1,579,357
_RS07480
BWI76_RS07485: BWI76_RS07485 - esterase, at 1,579,511 to 1,581,133
_RS07485
BWI76_RS07490: BWI76_RS07490 - hypothetical protein, at 1,581,062 to 1,581,310
_RS07490
BWI76_RS07495: BWI76_RS07495 - carbonate dehydratase, at 1,581,803 to 1,584,526
_RS07495
Position (kb)
1579
1580
1581
1582 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1578.516 kb on - strand, within BWI76_RS07480 at 1578.624 kb on - strand, within BWI76_RS07480 at 1578.655 kb on - strand, within BWI76_RS07480 at 1578.678 kb on + strand, within BWI76_RS07480 at 1578.983 kb on - strand, within BWI76_RS07480 at 1579.678 kb on + strand, within BWI76_RS07485 at 1579.876 kb on + strand, within BWI76_RS07485 at 1579.926 kb on + strand, within BWI76_RS07485 at 1580.167 kb on + strand, within BWI76_RS07485 at 1580.175 kb on - strand, within BWI76_RS07485 at 1580.238 kb on + strand, within BWI76_RS07485 at 1580.266 kb on - strand, within BWI76_RS07485 at 1580.279 kb on + strand, within BWI76_RS07485 at 1580.314 kb on + strand, within BWI76_RS07485 at 1580.436 kb on - strand, within BWI76_RS07485 at 1580.765 kb on - strand, within BWI76_RS07485 at 1580.970 kb on + strand, within BWI76_RS07485 at 1580.978 kb on - strand at 1580.978 kb on - strand at 1580.982 kb on + strand at 1580.992 kb on - strand at 1581.007 kb on + strand at 1581.015 kb on - strand at 1581.015 kb on - strand at 1581.029 kb on + strand at 1581.154 kb on + strand, within BWI76_RS07490 at 1581.156 kb on + strand, within BWI76_RS07490 at 1581.156 kb on + strand, within BWI76_RS07490 at 1581.156 kb on + strand, within BWI76_RS07490 at 1581.164 kb on - strand, within BWI76_RS07490 at 1581.164 kb on - strand, within BWI76_RS07490 at 1581.174 kb on - strand, within BWI76_RS07490 at 1581.184 kb on - strand, within BWI76_RS07490 at 1581.211 kb on + strand, within BWI76_RS07490 at 1581.213 kb on + strand, within BWI76_RS07490 at 1581.219 kb on - strand, within BWI76_RS07490 at 1581.225 kb on - strand, within BWI76_RS07490 at 1581.254 kb on - strand, within BWI76_RS07490 at 1581.259 kb on - strand, within BWI76_RS07490 at 1581.279 kb on + strand, within BWI76_RS07490 at 1581.279 kb on + strand, within BWI76_RS07490 at 1581.281 kb on + strand, within BWI76_RS07490 at 1581.287 kb on - strand at 1581.287 kb on - strand at 1581.287 kb on - strand at 1581.353 kb on - strand at 1581.361 kb on + strand at 1581.361 kb on + strand at 1581.369 kb on - strand at 1581.393 kb on + strand at 1581.503 kb on - strand at 1581.527 kb on - strand at 1581.527 kb on - strand at 1581.535 kb on - strand at 1581.565 kb on + strand at 1581.566 kb on + strand at 1581.574 kb on + strand at 1581.574 kb on + strand at 1581.575 kb on - strand at 1581.577 kb on - strand at 1581.578 kb on - strand at 1581.582 kb on - strand at 1581.582 kb on - strand at 1581.582 kb on - strand at 1581.582 kb on - strand at 1581.582 kb on - strand at 1581.582 kb on - strand at 1581.582 kb on - strand at 1581.597 kb on + strand at 1581.605 kb on - strand at 1581.605 kb on - strand at 1581.615 kb on - strand at 1581.615 kb on - strand at 1581.730 kb on + strand at 1581.750 kb on + strand at 1581.755 kb on + strand at 1581.759 kb on + strand at 1581.768 kb on - strand at 1581.795 kb on + strand at 1581.800 kb on + strand at 1581.837 kb on + strand at 1581.837 kb on + strand at 1581.837 kb on + strand at 1581.837 kb on + strand at 1581.845 kb on - strand at 1581.851 kb on + strand at 1581.851 kb on + strand at 1581.859 kb on - strand at 1581.859 kb on - strand at 1581.861 kb on + strand at 1581.864 kb on + strand at 1581.864 kb on + strand at 1581.864 kb on + strand at 1581.864 kb on + strand at 1581.864 kb on + strand at 1581.866 kb on + strand at 1581.866 kb on + strand at 1581.869 kb on - strand at 1581.872 kb on - strand at 1581.872 kb on - strand at 1581.872 kb on - strand at 1581.874 kb on - strand at 1581.874 kb on - strand at 1581.876 kb on + strand at 1581.876 kb on + strand at 1581.879 kb on - strand at 1581.884 kb on - strand at 1581.884 kb on - strand at 1581.885 kb on - strand at 1581.887 kb on + strand at 1581.895 kb on - strand at 1581.895 kb on - strand at 1581.898 kb on + strand at 1581.906 kb on - strand at 1581.906 kb on - strand at 1581.906 kb on - strand at 1581.906 kb on - strand at 1581.906 kb on - strand at 1581.908 kb on - strand at 1581.935 kb on + strand at 1582.018 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.5 mM remove 1,578,516 - BWI76_RS07480 0.61 -0.4 1,578,624 - BWI76_RS07480 0.66 -1.9 1,578,655 - BWI76_RS07480 0.67 -0.1 1,578,678 + BWI76_RS07480 0.68 -2.1 1,578,983 - BWI76_RS07480 0.83 -1.0 1,579,678 + BWI76_RS07485 0.10 -2.1 1,579,876 + BWI76_RS07485 0.22 -0.1 1,579,926 + BWI76_RS07485 0.26 +0.1 1,580,167 + BWI76_RS07485 0.40 -0.5 1,580,175 - BWI76_RS07485 0.41 -0.5 1,580,238 + BWI76_RS07485 0.45 -0.7 1,580,266 - BWI76_RS07485 0.47 -1.3 1,580,279 + BWI76_RS07485 0.47 -0.3 1,580,314 + BWI76_RS07485 0.49 -0.8 1,580,436 - BWI76_RS07485 0.57 +0.9 1,580,765 - BWI76_RS07485 0.77 -0.3 1,580,970 + BWI76_RS07485 0.90 -0.5 1,580,978 - +0.8 1,580,978 - +0.4 1,580,982 + -0.6 1,580,992 - -0.3 1,581,007 + +0.7 1,581,015 - +0.0 1,581,015 - +1.1 1,581,029 + +0.1 1,581,154 + BWI76_RS07490 0.37 -0.3 1,581,156 + BWI76_RS07490 0.38 -0.4 1,581,156 + BWI76_RS07490 0.38 +0.5 1,581,156 + BWI76_RS07490 0.38 +1.5 1,581,164 - BWI76_RS07490 0.41 -1.5 1,581,164 - BWI76_RS07490 0.41 -0.7 1,581,174 - BWI76_RS07490 0.45 +1.2 1,581,184 - BWI76_RS07490 0.49 -0.2 1,581,211 + BWI76_RS07490 0.60 +2.2 1,581,213 + BWI76_RS07490 0.61 -1.1 1,581,219 - BWI76_RS07490 0.63 -0.5 1,581,225 - BWI76_RS07490 0.65 +1.8 1,581,254 - BWI76_RS07490 0.77 -0.3 1,581,259 - BWI76_RS07490 0.79 +1.5 1,581,279 + BWI76_RS07490 0.87 +0.2 1,581,279 + BWI76_RS07490 0.87 +0.1 1,581,281 + BWI76_RS07490 0.88 +0.0 1,581,287 - -0.0 1,581,287 - -0.5 1,581,287 - +1.0 1,581,353 - -2.9 1,581,361 + +1.4 1,581,361 + +1.3 1,581,369 - -1.1 1,581,393 + +0.8 1,581,503 - +0.6 1,581,527 - -1.1 1,581,527 - -1.0 1,581,535 - +0.2 1,581,565 + -1.7 1,581,566 + -2.3 1,581,574 + +1.6 1,581,574 + -2.5 1,581,575 - -0.1 1,581,577 - -0.7 1,581,578 - +2.8 1,581,582 - -1.5 1,581,582 - +0.1 1,581,582 - +1.0 1,581,582 - +0.8 1,581,582 - +0.6 1,581,582 - +0.9 1,581,582 - +0.8 1,581,597 + +1.2 1,581,605 - -0.2 1,581,605 - -0.5 1,581,615 - +1.6 1,581,615 - -2.0 1,581,730 + -0.4 1,581,750 + +0.4 1,581,755 + +0.3 1,581,759 + -1.1 1,581,768 - -0.7 1,581,795 + -2.0 1,581,800 + -0.7 1,581,837 + -2.5 1,581,837 + -1.1 1,581,837 + -3.5 1,581,837 + +0.7 1,581,845 - +0.8 1,581,851 + -0.1 1,581,851 + -1.1 1,581,859 - -1.3 1,581,859 - -2.5 1,581,861 + +2.2 1,581,864 + -0.7 1,581,864 + +0.8 1,581,864 + -1.1 1,581,864 + -0.8 1,581,864 + -2.0 1,581,866 + -1.5 1,581,866 + -0.5 1,581,869 - +1.5 1,581,872 - -0.7 1,581,872 - +1.1 1,581,872 - +0.2 1,581,874 - -1.7 1,581,874 - +0.7 1,581,876 + +1.8 1,581,876 + -0.8 1,581,879 - +1.0 1,581,884 - -0.4 1,581,884 - -0.1 1,581,885 - -0.9 1,581,887 + -0.1 1,581,895 - -0.4 1,581,895 - -0.9 1,581,898 + -1.2 1,581,906 - +1.1 1,581,906 - +2.0 1,581,906 - -1.7 1,581,906 - -0.3 1,581,906 - -2.0 1,581,908 - -1.2 1,581,935 + -0.4 1,582,018 + +1.3
Or see this region's nucleotide sequence