Experiment: Cobalt chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05455 and BWI76_RS05460 are separated by 132 nucleotides BWI76_RS05460 and BWI76_RS05465 are separated by 245 nucleotides BWI76_RS05465 and BWI76_RS05470 are separated by 182 nucleotides
BWI76_RS05455: BWI76_RS05455 - DNA polymerase III subunit epsilon, at 1,144,495 to 1,145,226
_RS05455
BWI76_RS05460: BWI76_RS05460 - tRNA-Asp, at 1,145,359 to 1,145,435
_RS05460
BWI76_RS05465: BWI76_RS05465 - amidohydrolase, at 1,145,681 to 1,146,451
_RS05465
BWI76_RS05470: BWI76_RS05470 - acyl-CoA dehydrogenase, at 1,146,634 to 1,149,078
_RS05470
Position (kb)
1145
1146
1147 Strain fitness (log2 ratio)
-2
-1
0
1 at 1145.308 kb on + strand at 1145.520 kb on + strand at 1145.628 kb on - strand at 1145.997 kb on - strand, within BWI76_RS05465 at 1146.396 kb on - strand at 1146.490 kb on + strand at 1146.982 kb on - strand, within BWI76_RS05470 at 1147.030 kb on + strand, within BWI76_RS05470 at 1147.132 kb on - strand, within BWI76_RS05470 at 1147.231 kb on + strand, within BWI76_RS05470 at 1147.317 kb on + strand, within BWI76_RS05470 at 1147.347 kb on - strand, within BWI76_RS05470 at 1147.395 kb on - strand, within BWI76_RS05470 at 1147.421 kb on - strand, within BWI76_RS05470 at 1147.428 kb on + strand, within BWI76_RS05470
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.5 mM remove 1,145,308 + -0.1 1,145,520 + +0.8 1,145,628 - -0.4 1,145,997 - BWI76_RS05465 0.41 -2.1 1,146,396 - -1.5 1,146,490 + +0.6 1,146,982 - BWI76_RS05470 0.14 +0.1 1,147,030 + BWI76_RS05470 0.16 -0.1 1,147,132 - BWI76_RS05470 0.20 +0.4 1,147,231 + BWI76_RS05470 0.24 -1.4 1,147,317 + BWI76_RS05470 0.28 -0.3 1,147,347 - BWI76_RS05470 0.29 +1.0 1,147,395 - BWI76_RS05470 0.31 +0.5 1,147,421 - BWI76_RS05470 0.32 +0.1 1,147,428 + BWI76_RS05470 0.32 -1.2
Or see this region's nucleotide sequence