Experiment: Cobalt chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00220 and BWI76_RS00225 are separated by 616 nucleotides BWI76_RS00225 and BWI76_RS00230 are separated by 112 nucleotides
BWI76_RS00220: BWI76_RS00220 - F0F1 ATP synthase subunit I, at 44,948 to 45,328
_RS00220
BWI76_RS00225: BWI76_RS00225 - 16S rRNA methyltransferase G, at 45,945 to 46,568
_RS00225
BWI76_RS00230: BWI76_RS00230 - tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA, at 46,681 to 48,570
_RS00230
Position (kb) 45
46
47 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 45.090 kb on - strand, within BWI76_RS00220 at 45.115 kb on - strand, within BWI76_RS00220 at 45.518 kb on - strand at 45.684 kb on + strand at 45.818 kb on - strand at 45.858 kb on + strand at 45.968 kb on + strand at 45.968 kb on + strand at 45.981 kb on - strand at 45.985 kb on + strand at 45.985 kb on + strand at 45.985 kb on + strand at 45.993 kb on - strand at 45.997 kb on + strand at 46.005 kb on - strand at 46.064 kb on + strand, within BWI76_RS00225 at 46.064 kb on + strand, within BWI76_RS00225 at 46.077 kb on - strand, within BWI76_RS00225 at 46.203 kb on + strand, within BWI76_RS00225 at 46.208 kb on + strand, within BWI76_RS00225 at 46.211 kb on - strand, within BWI76_RS00225 at 46.211 kb on - strand, within BWI76_RS00225 at 46.271 kb on + strand, within BWI76_RS00225 at 46.279 kb on - strand, within BWI76_RS00225 at 46.290 kb on + strand, within BWI76_RS00225 at 46.337 kb on + strand, within BWI76_RS00225 at 46.372 kb on + strand, within BWI76_RS00225 at 46.380 kb on - strand, within BWI76_RS00225 at 46.427 kb on + strand, within BWI76_RS00225 at 46.427 kb on + strand, within BWI76_RS00225 at 46.471 kb on - strand, within BWI76_RS00225 at 46.523 kb on + strand at 46.531 kb on - strand at 46.578 kb on - strand at 46.595 kb on + strand at 46.641 kb on + strand at 46.681 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.5 mM remove 45,090 - BWI76_RS00220 0.37 +0.8 45,115 - BWI76_RS00220 0.44 +0.5 45,518 - +1.7 45,684 + -0.8 45,818 - +0.2 45,858 + -1.1 45,968 + -0.3 45,968 + +0.2 45,981 - +0.3 45,985 + -2.3 45,985 + -3.9 45,985 + +2.7 45,993 - -2.4 45,997 + -1.6 46,005 - -2.8 46,064 + BWI76_RS00225 0.19 -1.8 46,064 + BWI76_RS00225 0.19 -1.2 46,077 - BWI76_RS00225 0.21 -0.5 46,203 + BWI76_RS00225 0.41 -1.2 46,208 + BWI76_RS00225 0.42 +0.6 46,211 - BWI76_RS00225 0.43 -1.1 46,211 - BWI76_RS00225 0.43 -1.0 46,271 + BWI76_RS00225 0.52 -3.0 46,279 - BWI76_RS00225 0.54 -0.6 46,290 + BWI76_RS00225 0.55 -0.1 46,337 + BWI76_RS00225 0.63 -1.6 46,372 + BWI76_RS00225 0.68 -0.3 46,380 - BWI76_RS00225 0.70 -1.7 46,427 + BWI76_RS00225 0.77 -4.5 46,427 + BWI76_RS00225 0.77 -0.6 46,471 - BWI76_RS00225 0.84 -1.8 46,523 + +1.1 46,531 - -3.8 46,578 - -1.1 46,595 + -1.2 46,641 + -1.0 46,681 - +4.8
Or see this region's nucleotide sequence