Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS19370

Experiment: Nickel (II) chloride 1.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS19365 and BWI76_RS19370 are separated by 438 nucleotidesBWI76_RS19370 and BWI76_RS19375 are separated by 148 nucleotidesBWI76_RS19375 and BWI76_RS19380 overlap by 4 nucleotides BWI76_RS19365: BWI76_RS19365 - ribulokinase, at 4,035,482 to 4,037,089 _RS19365 BWI76_RS19370: BWI76_RS19370 - fructose-bisphosphate aldolase, at 4,037,528 to 4,038,580 _RS19370 BWI76_RS19375: BWI76_RS19375 - bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase, at 4,038,729 to 4,039,529 _RS19375 BWI76_RS19380: BWI76_RS19380 - hydroxyethylthiazole kinase, at 4,039,526 to 4,040,299 _RS19380 Position (kb) 4037 4038 4039Strain fitness (log2 ratio) -3 -2 -1 0 1at 4036.947 kb on + strandat 4036.975 kb on + strandat 4037.014 kb on + strandat 4037.014 kb on + strandat 4037.014 kb on + strandat 4037.176 kb on - strandat 4037.176 kb on - strandat 4037.329 kb on - strandat 4037.397 kb on - strandat 4037.408 kb on + strandat 4037.571 kb on + strandat 4037.627 kb on + strandat 4037.820 kb on - strand, within BWI76_RS19370at 4038.028 kb on - strand, within BWI76_RS19370at 4038.091 kb on + strand, within BWI76_RS19370at 4038.126 kb on - strand, within BWI76_RS19370at 4038.128 kb on + strand, within BWI76_RS19370at 4038.197 kb on + strand, within BWI76_RS19370at 4038.347 kb on + strand, within BWI76_RS19370at 4038.407 kb on + strand, within BWI76_RS19370at 4038.413 kb on - strand, within BWI76_RS19370at 4038.645 kb on + strandat 4038.645 kb on + strandat 4038.645 kb on + strandat 4038.645 kb on + strandat 4038.653 kb on - strandat 4038.653 kb on - strandat 4038.818 kb on - strand, within BWI76_RS19375at 4038.976 kb on - strand, within BWI76_RS19375at 4039.044 kb on + strand, within BWI76_RS19375at 4039.493 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nickel (II) chloride 1.5 mM
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4,036,947 + +0.2
4,036,975 + -0.4
4,037,014 + -1.5
4,037,014 + -0.1
4,037,014 + -0.0
4,037,176 - -0.5
4,037,176 - +0.1
4,037,329 - -1.2
4,037,397 - +0.7
4,037,408 + +0.4
4,037,571 + -0.7
4,037,627 + -2.9
4,037,820 - BWI76_RS19370 0.28 -1.3
4,038,028 - BWI76_RS19370 0.47 -1.2
4,038,091 + BWI76_RS19370 0.53 -1.7
4,038,126 - BWI76_RS19370 0.57 -2.0
4,038,128 + BWI76_RS19370 0.57 -1.7
4,038,197 + BWI76_RS19370 0.64 +0.1
4,038,347 + BWI76_RS19370 0.78 -1.8
4,038,407 + BWI76_RS19370 0.83 -3.5
4,038,413 - BWI76_RS19370 0.84 -1.4
4,038,645 + -0.5
4,038,645 + -1.6
4,038,645 + -3.0
4,038,645 + -1.8
4,038,653 - -0.9
4,038,653 - -1.0
4,038,818 - BWI76_RS19375 0.11 -2.3
4,038,976 - BWI76_RS19375 0.31 +0.3
4,039,044 + BWI76_RS19375 0.39 +0.5
4,039,493 - +0.1

Or see this region's nucleotide sequence