Experiment: Nickel (II) chloride 1.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11930 and BWI76_RS11935 are separated by 245 nucleotides BWI76_RS11935 and BWI76_RS11940 are separated by 394 nucleotides BWI76_RS11940 and BWI76_RS11945 are separated by 56 nucleotides BWI76_RS11945 and BWI76_RS11950 are separated by 161 nucleotides
BWI76_RS11930: BWI76_RS11930 - LysR family transcriptional regulator CysB, at 2,511,557 to 2,512,531
_RS11930
BWI76_RS11935: BWI76_RS11935 - YmiA family putative membrane protein, at 2,512,777 to 2,512,944
_RS11935
BWI76_RS11940: BWI76_RS11940 - aconitate hydratase 1, at 2,513,339 to 2,516,011
_RS11940
BWI76_RS11945: BWI76_RS11945 - GTP cyclohydrolase II, at 2,516,068 to 2,516,658
_RS11945
BWI76_RS11950: BWI76_RS11950 - phosphatidylglycerophosphatase B, at 2,516,820 to 2,517,587
_RS11950
Position (kb)
2513
2514
2515
2516
2517 Strain fitness (log2 ratio)
-2
-1
0
1 at 2512.580 kb on - strand at 2512.681 kb on + strand at 2512.860 kb on + strand, within BWI76_RS11935 at 2512.871 kb on + strand, within BWI76_RS11935 at 2512.893 kb on + strand, within BWI76_RS11935 at 2512.904 kb on + strand, within BWI76_RS11935 at 2512.904 kb on + strand at 2512.910 kb on + strand, within BWI76_RS11935 at 2512.949 kb on + strand at 2513.077 kb on + strand at 2513.131 kb on - strand at 2513.148 kb on + strand at 2513.151 kb on + strand at 2513.156 kb on - strand at 2513.159 kb on - strand at 2513.159 kb on - strand at 2513.159 kb on - strand at 2513.159 kb on - strand at 2513.159 kb on - strand at 2513.164 kb on - strand at 2513.173 kb on - strand at 2513.174 kb on + strand at 2513.174 kb on - strand at 2513.174 kb on - strand at 2513.174 kb on - strand at 2513.220 kb on + strand at 2513.234 kb on - strand at 2513.301 kb on + strand at 2513.310 kb on - strand at 2513.324 kb on + strand at 2513.326 kb on - strand at 2513.331 kb on - strand at 2513.332 kb on - strand at 2513.357 kb on - strand at 2513.380 kb on + strand at 2513.468 kb on + strand at 2513.889 kb on + strand, within BWI76_RS11940 at 2514.098 kb on - strand, within BWI76_RS11940 at 2514.098 kb on - strand at 2514.210 kb on + strand, within BWI76_RS11940 at 2514.363 kb on - strand, within BWI76_RS11940 at 2514.446 kb on + strand, within BWI76_RS11940 at 2514.819 kb on - strand, within BWI76_RS11940 at 2515.148 kb on + strand, within BWI76_RS11940 at 2515.153 kb on - strand, within BWI76_RS11940 at 2515.326 kb on - strand, within BWI76_RS11940 at 2515.761 kb on + strand at 2516.688 kb on - strand at 2516.753 kb on - strand at 2516.942 kb on + strand, within BWI76_RS11950 at 2516.947 kb on - strand, within BWI76_RS11950
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1.5 mM remove 2,512,580 - +0.3 2,512,681 + +0.7 2,512,860 + BWI76_RS11935 0.49 +0.9 2,512,871 + BWI76_RS11935 0.56 -1.9 2,512,893 + BWI76_RS11935 0.69 -0.4 2,512,904 + BWI76_RS11935 0.76 -0.6 2,512,904 + +0.6 2,512,910 + BWI76_RS11935 0.79 -1.8 2,512,949 + +0.3 2,513,077 + -0.4 2,513,131 - -0.5 2,513,148 + +0.7 2,513,151 + -0.5 2,513,156 - -1.0 2,513,159 - -0.4 2,513,159 - -0.1 2,513,159 - +0.2 2,513,159 - +0.1 2,513,159 - -0.9 2,513,164 - +0.5 2,513,173 - -0.2 2,513,174 + -1.0 2,513,174 - -0.7 2,513,174 - +0.1 2,513,174 - -0.1 2,513,220 + -0.0 2,513,234 - -0.3 2,513,301 + +1.1 2,513,310 - -0.8 2,513,324 + -0.5 2,513,326 - -1.2 2,513,331 - -0.7 2,513,332 - -1.2 2,513,357 - -1.4 2,513,380 + -2.7 2,513,468 + -1.6 2,513,889 + BWI76_RS11940 0.21 -1.4 2,514,098 - BWI76_RS11940 0.28 -0.9 2,514,098 - +0.3 2,514,210 + BWI76_RS11940 0.33 -0.2 2,514,363 - BWI76_RS11940 0.38 +0.7 2,514,446 + BWI76_RS11940 0.41 -1.0 2,514,819 - BWI76_RS11940 0.55 -2.1 2,515,148 + BWI76_RS11940 0.68 -2.6 2,515,153 - BWI76_RS11940 0.68 -2.1 2,515,326 - BWI76_RS11940 0.74 -1.6 2,515,761 + -0.4 2,516,688 - +1.3 2,516,753 - -0.3 2,516,942 + BWI76_RS11950 0.16 +0.3 2,516,947 - BWI76_RS11950 0.17 +0.3
Or see this region's nucleotide sequence