Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_648

Experiment: Zinc chloride 0.0000015625 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_646 and Pf6N2E2_647 are separated by 147 nucleotidesPf6N2E2_647 and Pf6N2E2_648 are separated by 47 nucleotidesPf6N2E2_648 and Pf6N2E2_649 overlap by 4 nucleotides Pf6N2E2_646 - General secretion pathway protein I, at 735,700 to 736,074 _646 Pf6N2E2_647 - General secretion pathway protein G, at 736,222 to 736,671 _647 Pf6N2E2_648 - General secretion pathway protein K, at 736,719 to 737,666 _648 Pf6N2E2_649 - General secretion pathway protein L, at 737,663 to 738,835 _649 Position (kb) 736 737 738Strain fitness (log2 ratio) -2 -1 0 1at 736.159 kb on + strandat 736.171 kb on - strandat 736.171 kb on - strandat 736.171 kb on - strandat 736.519 kb on + strand, within Pf6N2E2_647at 736.559 kb on - strand, within Pf6N2E2_647at 736.564 kb on + strand, within Pf6N2E2_647at 736.565 kb on - strand, within Pf6N2E2_647at 736.670 kb on - strandat 736.670 kb on - strandat 737.400 kb on + strand, within Pf6N2E2_648at 737.468 kb on + strand, within Pf6N2E2_648at 737.618 kb on + strandat 737.662 kb on + strandat 738.077 kb on + strand, within Pf6N2E2_649at 738.113 kb on - strand, within Pf6N2E2_649at 738.413 kb on + strand, within Pf6N2E2_649

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Per-strain Table

Position Strand Gene LocusTag Fraction Zinc chloride 0.0000015625 M
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736,159 + -1.4
736,171 - -2.6
736,171 - +0.3
736,171 - -1.0
736,519 + Pf6N2E2_647 0.66 +1.5
736,559 - Pf6N2E2_647 0.75 -0.9
736,564 + Pf6N2E2_647 0.76 +0.9
736,565 - Pf6N2E2_647 0.76 -0.3
736,670 - -0.6
736,670 - -1.2
737,400 + Pf6N2E2_648 0.72 -0.7
737,468 + Pf6N2E2_648 0.79 -2.5
737,618 + -1.1
737,662 + +0.0
738,077 + Pf6N2E2_649 0.35 +1.4
738,113 - Pf6N2E2_649 0.38 -1.6
738,413 + Pf6N2E2_649 0.64 +0.7

Or see this region's nucleotide sequence