Experiment: Zinc chloride 0.0000015625 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_55 and Pf6N2E2_56 are separated by 45 nucleotides Pf6N2E2_56 and Pf6N2E2_57 overlap by 43 nucleotides Pf6N2E2_57 and Pf6N2E2_58 overlap by 6 nucleotides Pf6N2E2_58 and Pf6N2E2_59 are separated by 120 nucleotides Pf6N2E2_59 and Pf6N2E2_60 are separated by 193 nucleotides
Pf6N2E2_55 - Probable hydrolase, at 95,444 to 96,277
_55
Pf6N2E2_56 - FIG002571: 4-hydroxybenzoyl-CoA thioesterase domain protein, at 96,323 to 96,772
_56
Pf6N2E2_57 - hypothetical protein, at 96,730 to 96,891
_57
Pf6N2E2_58 - tRNA-dihydrouridine synthase C (EC 1.-.-.-), at 96,886 to 97,845
_58
Pf6N2E2_59 - 16 kDa heat shock protein A, at 97,966 to 98,418
_59
Pf6N2E2_60 - Sensory box/GGDEF family protein, at 98,612 to 101,890
_60
Position (kb) 96
97
98 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 96.352 kb on + strand at 96.367 kb on - strand at 96.411 kb on + strand, within Pf6N2E2_56 at 96.412 kb on - strand, within Pf6N2E2_56 at 96.413 kb on + strand, within Pf6N2E2_56 at 96.413 kb on + strand, within Pf6N2E2_56 at 96.413 kb on + strand, within Pf6N2E2_56 at 96.413 kb on + strand, within Pf6N2E2_56 at 96.413 kb on + strand, within Pf6N2E2_56 at 96.413 kb on + strand, within Pf6N2E2_56 at 96.413 kb on + strand, within Pf6N2E2_56 at 96.413 kb on + strand, within Pf6N2E2_56 at 96.414 kb on - strand, within Pf6N2E2_56 at 96.414 kb on - strand at 96.414 kb on - strand, within Pf6N2E2_56 at 96.414 kb on - strand, within Pf6N2E2_56 at 96.414 kb on - strand, within Pf6N2E2_56 at 96.543 kb on - strand, within Pf6N2E2_56 at 96.543 kb on - strand, within Pf6N2E2_56 at 96.706 kb on + strand, within Pf6N2E2_56 at 96.707 kb on - strand, within Pf6N2E2_56 at 96.707 kb on - strand, within Pf6N2E2_56 at 96.707 kb on - strand, within Pf6N2E2_56 at 97.377 kb on + strand, within Pf6N2E2_58 at 97.411 kb on + strand, within Pf6N2E2_58 at 97.411 kb on + strand, within Pf6N2E2_58 at 97.889 kb on + strand at 97.889 kb on + strand at 97.889 kb on + strand at 97.889 kb on + strand at 97.889 kb on + strand at 97.889 kb on + strand at 97.889 kb on + strand at 97.894 kb on - strand at 97.894 kb on - strand at 97.971 kb on + strand at 98.072 kb on - strand, within Pf6N2E2_59 at 98.072 kb on - strand, within Pf6N2E2_59 at 98.080 kb on + strand, within Pf6N2E2_59 at 98.080 kb on + strand, within Pf6N2E2_59 at 98.080 kb on + strand, within Pf6N2E2_59 at 98.080 kb on + strand, within Pf6N2E2_59 at 98.081 kb on - strand, within Pf6N2E2_59 at 98.227 kb on + strand, within Pf6N2E2_59 at 98.227 kb on + strand, within Pf6N2E2_59 at 98.228 kb on - strand, within Pf6N2E2_59 at 98.462 kb on + strand at 98.468 kb on + strand at 98.468 kb on + strand at 98.469 kb on - strand at 98.469 kb on - strand at 98.531 kb on - strand at 98.531 kb on - strand at 98.531 kb on - strand at 98.531 kb on - strand at 98.546 kb on + strand at 98.547 kb on - strand at 98.547 kb on - strand at 98.547 kb on - strand at 98.614 kb on - strand at 98.664 kb on + strand at 98.664 kb on + strand at 98.665 kb on - strand at 98.673 kb on + strand at 98.674 kb on - strand at 98.674 kb on - strand at 98.674 kb on - strand at 98.675 kb on + strand at 98.675 kb on + strand at 98.675 kb on + strand at 98.675 kb on + strand at 98.675 kb on + strand at 98.675 kb on + strand at 98.675 kb on + strand at 98.675 kb on + strand at 98.675 kb on + strand at 98.675 kb on + strand at 98.675 kb on + strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Zinc chloride 0.0000015625 M remove 96,352 + +1.0 96,367 - +1.3 96,411 + Pf6N2E2_56 0.20 -0.4 96,412 - Pf6N2E2_56 0.20 -0.2 96,413 + Pf6N2E2_56 0.20 +1.4 96,413 + Pf6N2E2_56 0.20 +0.9 96,413 + Pf6N2E2_56 0.20 -2.7 96,413 + Pf6N2E2_56 0.20 -1.7 96,413 + Pf6N2E2_56 0.20 +1.2 96,413 + Pf6N2E2_56 0.20 -0.7 96,413 + Pf6N2E2_56 0.20 -1.0 96,413 + Pf6N2E2_56 0.20 -1.1 96,414 - Pf6N2E2_56 0.20 +2.1 96,414 - +0.9 96,414 - Pf6N2E2_56 0.20 +1.2 96,414 - Pf6N2E2_56 0.20 -0.1 96,414 - Pf6N2E2_56 0.20 -1.0 96,543 - Pf6N2E2_56 0.49 -1.3 96,543 - Pf6N2E2_56 0.49 +0.5 96,706 + Pf6N2E2_56 0.85 +0.5 96,707 - Pf6N2E2_56 0.85 -0.3 96,707 - Pf6N2E2_56 0.85 -0.1 96,707 - Pf6N2E2_56 0.85 -1.1 97,377 + Pf6N2E2_58 0.51 -0.6 97,411 + Pf6N2E2_58 0.55 +0.8 97,411 + Pf6N2E2_58 0.55 +1.1 97,889 + -0.1 97,889 + +1.3 97,889 + +2.5 97,889 + -0.9 97,889 + -0.7 97,889 + -3.1 97,889 + -0.8 97,894 - +0.9 97,894 - +1.4 97,971 + +1.4 98,072 - Pf6N2E2_59 0.23 +0.2 98,072 - Pf6N2E2_59 0.23 +1.2 98,080 + Pf6N2E2_59 0.25 -2.8 98,080 + Pf6N2E2_59 0.25 -0.2 98,080 + Pf6N2E2_59 0.25 +2.1 98,080 + Pf6N2E2_59 0.25 +1.1 98,081 - Pf6N2E2_59 0.25 +1.0 98,227 + Pf6N2E2_59 0.58 +0.1 98,227 + Pf6N2E2_59 0.58 +1.3 98,228 - Pf6N2E2_59 0.58 +1.5 98,462 + +2.3 98,468 + +3.0 98,468 + -0.7 98,469 - +0.5 98,469 - -1.8 98,531 - -0.0 98,531 - -0.7 98,531 - +1.1 98,531 - -2.5 98,546 + -0.2 98,547 - +2.0 98,547 - -0.2 98,547 - +0.3 98,614 - +0.8 98,664 + +0.1 98,664 + +0.2 98,665 - -0.4 98,673 + +1.4 98,674 - +1.9 98,674 - +0.3 98,674 - -0.3 98,675 + -0.8 98,675 + +1.3 98,675 + -2.7 98,675 + -0.2 98,675 + +2.0 98,675 + -1.7 98,675 + -0.4 98,675 + +1.0 98,675 + -1.5 98,675 + +0.1 98,675 + +1.3 98,676 - +2.5 98,676 - +2.5 98,676 - +0.2 98,676 - +1.2 98,676 - -2.7 98,676 - -2.7 98,676 - -0.3 98,676 - -0.2 98,676 - +0.9 98,676 - -1.6 98,676 - -0.7 98,676 - -2.5 98,676 - +2.6 98,676 - +0.8
Or see this region's nucleotide sequence