Experiment: Zinc chloride 0.0000015625 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_501 and Pf6N2E2_502 are separated by 239 nucleotides Pf6N2E2_502 and Pf6N2E2_503 are separated by 185 nucleotides Pf6N2E2_503 and Pf6N2E2_504 overlap by 23 nucleotides Pf6N2E2_504 and Pf6N2E2_505 are separated by 24 nucleotides Pf6N2E2_505 and Pf6N2E2_506 overlap by 35 nucleotides
Pf6N2E2_501 - Sensory box transcriptional regulator, LuxR family, at 570,546 to 572,030
_501
Pf6N2E2_502 - hypothetical protein, at 572,270 to 572,515
_502
Pf6N2E2_503 - sensor histidine kinase, at 572,701 to 574,086
_503
Pf6N2E2_504 - DNA-binding response regulator, at 574,064 to 574,747
_504
Pf6N2E2_505 - hypothetical protein, at 574,772 to 575,053
_505
Pf6N2E2_506 - Hydrogen cyanide synthase HcnA / Opine oxidase subunit C, at 575,019 to 575,342
_506
Position (kb) 572
573
574
575 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 571.908 kb on - strand at 571.929 kb on - strand at 571.935 kb on + strand at 571.996 kb on + strand at 572.177 kb on + strand at 572.178 kb on - strand at 572.185 kb on + strand at 572.186 kb on - strand at 572.186 kb on - strand at 572.205 kb on + strand at 572.205 kb on + strand at 572.205 kb on + strand at 572.206 kb on - strand at 572.364 kb on + strand, within Pf6N2E2_502 at 572.372 kb on + strand, within Pf6N2E2_502 at 572.373 kb on - strand, within Pf6N2E2_502 at 572.373 kb on - strand, within Pf6N2E2_502 at 572.474 kb on + strand, within Pf6N2E2_502 at 572.489 kb on - strand, within Pf6N2E2_502 at 572.561 kb on - strand at 572.561 kb on - strand at 572.561 kb on - strand at 572.637 kb on + strand at 572.703 kb on - strand at 572.761 kb on + strand at 572.761 kb on + strand at 572.761 kb on + strand at 572.855 kb on + strand, within Pf6N2E2_503 at 572.856 kb on - strand, within Pf6N2E2_503 at 573.025 kb on + strand, within Pf6N2E2_503 at 573.025 kb on + strand, within Pf6N2E2_503 at 573.025 kb on + strand, within Pf6N2E2_503 at 573.026 kb on - strand, within Pf6N2E2_503 at 573.110 kb on + strand, within Pf6N2E2_503 at 573.110 kb on + strand, within Pf6N2E2_503 at 573.133 kb on + strand, within Pf6N2E2_503 at 573.652 kb on + strand, within Pf6N2E2_503 at 573.967 kb on + strand at 573.968 kb on - strand at 573.968 kb on - strand at 573.974 kb on + strand at 573.974 kb on + strand at 573.974 kb on + strand at 573.975 kb on - strand at 573.975 kb on - strand at 573.977 kb on + strand at 573.977 kb on + strand at 573.977 kb on + strand at 573.977 kb on + strand at 573.977 kb on + strand at 573.978 kb on - strand at 573.978 kb on - strand at 573.978 kb on - strand at 573.978 kb on - strand at 573.978 kb on - strand at 574.025 kb on + strand at 574.026 kb on - strand at 574.026 kb on - strand at 574.026 kb on - strand at 574.197 kb on + strand, within Pf6N2E2_504 at 574.276 kb on - strand, within Pf6N2E2_504 at 574.361 kb on + strand, within Pf6N2E2_504 at 574.612 kb on - strand, within Pf6N2E2_504 at 574.776 kb on + strand at 574.776 kb on + strand at 574.802 kb on - strand, within Pf6N2E2_505 at 574.878 kb on + strand, within Pf6N2E2_505 at 574.878 kb on + strand, within Pf6N2E2_505 at 574.878 kb on + strand, within Pf6N2E2_505 at 574.890 kb on + strand, within Pf6N2E2_505 at 574.932 kb on + strand, within Pf6N2E2_505 at 574.933 kb on - strand, within Pf6N2E2_505 at 575.015 kb on + strand, within Pf6N2E2_505 at 575.015 kb on + strand, within Pf6N2E2_505 at 575.015 kb on + strand, within Pf6N2E2_505 at 575.015 kb on + strand, within Pf6N2E2_505 at 575.016 kb on - strand, within Pf6N2E2_505 at 575.016 kb on - strand, within Pf6N2E2_505 at 575.016 kb on - strand, within Pf6N2E2_505 at 575.016 kb on - strand, within Pf6N2E2_505 at 575.038 kb on + strand at 575.038 kb on + strand at 575.039 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Zinc chloride 0.0000015625 M remove 571,908 - -1.3 571,929 - +0.7 571,935 + +2.4 571,996 + -2.0 572,177 + +1.7 572,178 - +0.8 572,185 + -0.6 572,186 - +1.3 572,186 - +1.4 572,205 + +0.8 572,205 + -1.0 572,205 + -1.0 572,206 - +0.6 572,364 + Pf6N2E2_502 0.38 -1.9 572,372 + Pf6N2E2_502 0.41 +1.7 572,373 - Pf6N2E2_502 0.42 +1.4 572,373 - Pf6N2E2_502 0.42 -0.9 572,474 + Pf6N2E2_502 0.83 -0.7 572,489 - Pf6N2E2_502 0.89 -0.6 572,561 - -0.2 572,561 - +0.7 572,561 - -2.0 572,637 + -1.1 572,703 - -1.6 572,761 + +0.4 572,761 + -0.9 572,761 + -1.4 572,855 + Pf6N2E2_503 0.11 +0.4 572,856 - Pf6N2E2_503 0.11 +0.3 573,025 + Pf6N2E2_503 0.23 -1.9 573,025 + Pf6N2E2_503 0.23 +1.7 573,025 + Pf6N2E2_503 0.23 -1.3 573,026 - Pf6N2E2_503 0.23 +0.7 573,110 + Pf6N2E2_503 0.30 -2.5 573,110 + Pf6N2E2_503 0.30 -0.4 573,133 + Pf6N2E2_503 0.31 +0.9 573,652 + Pf6N2E2_503 0.69 +1.3 573,967 + -1.4 573,968 - -2.1 573,968 - +1.2 573,974 + -1.6 573,974 + +1.8 573,974 + -0.9 573,975 - -2.8 573,975 - +1.9 573,977 + +0.4 573,977 + -0.3 573,977 + +2.0 573,977 + -1.1 573,977 + +0.6 573,978 - -0.4 573,978 - -0.7 573,978 - -1.0 573,978 - -1.1 573,978 - -1.4 574,025 + -0.9 574,026 - +0.3 574,026 - -0.0 574,026 - -0.6 574,197 + Pf6N2E2_504 0.19 +2.4 574,276 - Pf6N2E2_504 0.31 -0.2 574,361 + Pf6N2E2_504 0.43 +1.3 574,612 - Pf6N2E2_504 0.80 -1.5 574,776 + -0.1 574,776 + +0.2 574,802 - Pf6N2E2_505 0.11 +1.9 574,878 + Pf6N2E2_505 0.38 +1.5 574,878 + Pf6N2E2_505 0.38 -1.8 574,878 + Pf6N2E2_505 0.38 +1.1 574,890 + Pf6N2E2_505 0.42 -0.8 574,932 + Pf6N2E2_505 0.57 -0.0 574,933 - Pf6N2E2_505 0.57 +1.1 575,015 + Pf6N2E2_505 0.86 -2.0 575,015 + Pf6N2E2_505 0.86 +2.3 575,015 + Pf6N2E2_505 0.86 +1.4 575,015 + Pf6N2E2_505 0.86 -0.3 575,016 - Pf6N2E2_505 0.87 +2.0 575,016 - Pf6N2E2_505 0.87 -0.4 575,016 - Pf6N2E2_505 0.87 +0.1 575,016 - Pf6N2E2_505 0.87 +0.8 575,038 + -0.2 575,038 + -0.2 575,039 - +1.1
Or see this region's nucleotide sequence