Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_481

Experiment: Zinc chloride 0.0000015625 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_480 and Pf6N2E2_481 are separated by 1 nucleotidesPf6N2E2_481 and Pf6N2E2_482 are separated by 192 nucleotidesPf6N2E2_482 and Pf6N2E2_483 are separated by 151 nucleotides Pf6N2E2_480 - 3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) (EC 1.2.4.4) (from data), at 546,161 to 547,219 _480 Pf6N2E2_481 - branched-chain alpha-ketoacid dehydrogenase, E1 component alpha subunit (EC 1.2.4.4) (from data), at 547,221 to 548,456 _481 Pf6N2E2_482 - Transcriptional regulator BkdR of isoleucine and valine catabolism operon, at 548,649 to 549,137 _482 Pf6N2E2_483 - FIG146518: Zn-dependent hydrolases, including glyoxylases, at 549,289 to 550,152 _483 Position (kb) 547 548 549Strain fitness (log2 ratio) -2 -1 0 1 2 3at 546.483 kb on + strand, within Pf6N2E2_480at 546.483 kb on + strand, within Pf6N2E2_480at 546.483 kb on + strand, within Pf6N2E2_480at 546.483 kb on + strand, within Pf6N2E2_480at 546.483 kb on + strand, within Pf6N2E2_480at 546.484 kb on - strand, within Pf6N2E2_480at 546.484 kb on - strand, within Pf6N2E2_480at 546.484 kb on - strand, within Pf6N2E2_480at 546.484 kb on - strand, within Pf6N2E2_480at 546.484 kb on - strand, within Pf6N2E2_480at 546.484 kb on - strand, within Pf6N2E2_480at 546.484 kb on - strand, within Pf6N2E2_480at 546.484 kb on - strand, within Pf6N2E2_480at 546.484 kb on - strand, within Pf6N2E2_480at 546.484 kb on - strand, within Pf6N2E2_480at 546.484 kb on - strand, within Pf6N2E2_480at 546.498 kb on + strand, within Pf6N2E2_480at 546.498 kb on + strand, within Pf6N2E2_480at 546.498 kb on + strand, within Pf6N2E2_480at 546.499 kb on - strand, within Pf6N2E2_480at 546.499 kb on - strand, within Pf6N2E2_480at 546.499 kb on - strandat 546.561 kb on + strand, within Pf6N2E2_480at 546.561 kb on + strand, within Pf6N2E2_480at 546.562 kb on - strand, within Pf6N2E2_480at 546.562 kb on - strand, within Pf6N2E2_480at 546.562 kb on - strand, within Pf6N2E2_480at 546.565 kb on - strand, within Pf6N2E2_480at 546.645 kb on + strand, within Pf6N2E2_480at 546.645 kb on + strand, within Pf6N2E2_480at 546.645 kb on + strand, within Pf6N2E2_480at 546.646 kb on - strand, within Pf6N2E2_480at 546.646 kb on - strand, within Pf6N2E2_480at 546.721 kb on - strand, within Pf6N2E2_480at 546.804 kb on + strand, within Pf6N2E2_480at 546.804 kb on + strand, within Pf6N2E2_480at 546.804 kb on + strand, within Pf6N2E2_480at 546.804 kb on + strand, within Pf6N2E2_480at 546.804 kb on + strand, within Pf6N2E2_480at 546.805 kb on - strand, within Pf6N2E2_480at 546.805 kb on - strand, within Pf6N2E2_480at 546.913 kb on - strand, within Pf6N2E2_480at 546.918 kb on + strand, within Pf6N2E2_480at 546.918 kb on + strand, within Pf6N2E2_480at 546.918 kb on + strand, within Pf6N2E2_480at 546.918 kb on + strand, within Pf6N2E2_480at 546.919 kb on - strand, within Pf6N2E2_480at 546.919 kb on - strand, within Pf6N2E2_480at 546.919 kb on - strand, within Pf6N2E2_480at 546.919 kb on - strand, within Pf6N2E2_480at 547.032 kb on + strand, within Pf6N2E2_480at 547.033 kb on - strand, within Pf6N2E2_480at 547.033 kb on - strand, within Pf6N2E2_480at 547.033 kb on - strand, within Pf6N2E2_480at 547.077 kb on + strand, within Pf6N2E2_480at 547.078 kb on - strandat 547.198 kb on + strandat 547.199 kb on - strandat 547.199 kb on - strandat 547.274 kb on - strandat 547.274 kb on - strandat 547.427 kb on + strand, within Pf6N2E2_481at 547.427 kb on + strand, within Pf6N2E2_481at 547.427 kb on + strand, within Pf6N2E2_481at 547.427 kb on + strand, within Pf6N2E2_481at 547.428 kb on - strand, within Pf6N2E2_481at 547.428 kb on - strand, within Pf6N2E2_481at 547.945 kb on + strand, within Pf6N2E2_481at 547.945 kb on + strand, within Pf6N2E2_481at 547.945 kb on + strand, within Pf6N2E2_481at 547.945 kb on + strand, within Pf6N2E2_481at 547.946 kb on - strand, within Pf6N2E2_481at 547.946 kb on - strand, within Pf6N2E2_481at 547.946 kb on - strand, within Pf6N2E2_481at 547.946 kb on - strand, within Pf6N2E2_481at 547.957 kb on - strand, within Pf6N2E2_481at 548.137 kb on + strand, within Pf6N2E2_481at 548.138 kb on - strand, within Pf6N2E2_481at 548.185 kb on + strand, within Pf6N2E2_481at 548.185 kb on + strandat 548.186 kb on - strand, within Pf6N2E2_481at 548.345 kb on + strandat 548.377 kb on + strandat 548.378 kb on - strandat 548.464 kb on + strandat 548.465 kb on - strandat 548.465 kb on - strandat 548.465 kb on - strandat 548.489 kb on + strandat 548.502 kb on - strandat 548.537 kb on + strandat 548.538 kb on - strandat 548.548 kb on + strandat 548.549 kb on - strandat 548.549 kb on - strandat 548.829 kb on - strand, within Pf6N2E2_482at 548.943 kb on + strand, within Pf6N2E2_482at 548.943 kb on + strand, within Pf6N2E2_482at 549.290 kb on + strandat 549.291 kb on - strandat 549.291 kb on - strandat 549.291 kb on - strandat 549.317 kb on + strandat 549.317 kb on + strandat 549.318 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Zinc chloride 0.0000015625 M
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546,483 + Pf6N2E2_480 0.30 +0.3
546,483 + Pf6N2E2_480 0.30 -2.0
546,483 + Pf6N2E2_480 0.30 +0.7
546,483 + Pf6N2E2_480 0.30 +1.3
546,483 + Pf6N2E2_480 0.30 +2.1
546,484 - Pf6N2E2_480 0.31 +0.5
546,484 - Pf6N2E2_480 0.31 +0.4
546,484 - Pf6N2E2_480 0.31 -0.1
546,484 - Pf6N2E2_480 0.31 -0.4
546,484 - Pf6N2E2_480 0.31 +1.9
546,484 - Pf6N2E2_480 0.31 -1.0
546,484 - Pf6N2E2_480 0.31 -0.6
546,484 - Pf6N2E2_480 0.31 +1.0
546,484 - Pf6N2E2_480 0.31 -1.0
546,484 - Pf6N2E2_480 0.31 +0.7
546,484 - Pf6N2E2_480 0.31 +1.1
546,498 + Pf6N2E2_480 0.32 -0.8
546,498 + Pf6N2E2_480 0.32 +1.0
546,498 + Pf6N2E2_480 0.32 -0.2
546,499 - Pf6N2E2_480 0.32 -0.5
546,499 - Pf6N2E2_480 0.32 +1.2
546,499 - +0.7
546,561 + Pf6N2E2_480 0.38 -1.2
546,561 + Pf6N2E2_480 0.38 +1.6
546,562 - Pf6N2E2_480 0.38 +1.8
546,562 - Pf6N2E2_480 0.38 -0.2
546,562 - Pf6N2E2_480 0.38 -0.8
546,565 - Pf6N2E2_480 0.38 -1.3
546,645 + Pf6N2E2_480 0.46 +1.2
546,645 + Pf6N2E2_480 0.46 +0.2
546,645 + Pf6N2E2_480 0.46 +2.0
546,646 - Pf6N2E2_480 0.46 -1.3
546,646 - Pf6N2E2_480 0.46 -0.4
546,721 - Pf6N2E2_480 0.53 +1.2
546,804 + Pf6N2E2_480 0.61 +0.1
546,804 + Pf6N2E2_480 0.61 +0.4
546,804 + Pf6N2E2_480 0.61 +0.8
546,804 + Pf6N2E2_480 0.61 -1.5
546,804 + Pf6N2E2_480 0.61 +0.3
546,805 - Pf6N2E2_480 0.61 +0.2
546,805 - Pf6N2E2_480 0.61 +1.9
546,913 - Pf6N2E2_480 0.71 -1.7
546,918 + Pf6N2E2_480 0.71 -1.6
546,918 + Pf6N2E2_480 0.71 -1.5
546,918 + Pf6N2E2_480 0.71 +1.1
546,918 + Pf6N2E2_480 0.71 -1.8
546,919 - Pf6N2E2_480 0.72 +1.7
546,919 - Pf6N2E2_480 0.72 -2.1
546,919 - Pf6N2E2_480 0.72 -1.3
546,919 - Pf6N2E2_480 0.72 +0.4
547,032 + Pf6N2E2_480 0.82 -0.4
547,033 - Pf6N2E2_480 0.82 -1.1
547,033 - Pf6N2E2_480 0.82 -0.6
547,033 - Pf6N2E2_480 0.82 +1.8
547,077 + Pf6N2E2_480 0.86 -0.9
547,078 - +0.7
547,198 + +1.6
547,199 - -1.3
547,199 - -0.9
547,274 - +1.3
547,274 - -0.3
547,427 + Pf6N2E2_481 0.17 -1.4
547,427 + Pf6N2E2_481 0.17 -0.1
547,427 + Pf6N2E2_481 0.17 -1.5
547,427 + Pf6N2E2_481 0.17 +0.4
547,428 - Pf6N2E2_481 0.17 -1.3
547,428 - Pf6N2E2_481 0.17 +2.3
547,945 + Pf6N2E2_481 0.59 -1.1
547,945 + Pf6N2E2_481 0.59 -1.4
547,945 + Pf6N2E2_481 0.59 -0.4
547,945 + Pf6N2E2_481 0.59 +3.3
547,946 - Pf6N2E2_481 0.59 -2.1
547,946 - Pf6N2E2_481 0.59 +0.3
547,946 - Pf6N2E2_481 0.59 -0.0
547,946 - Pf6N2E2_481 0.59 -1.3
547,957 - Pf6N2E2_481 0.60 +0.3
548,137 + Pf6N2E2_481 0.74 +0.2
548,138 - Pf6N2E2_481 0.74 -1.1
548,185 + Pf6N2E2_481 0.78 +1.3
548,185 + +0.7
548,186 - Pf6N2E2_481 0.78 -0.3
548,345 + -1.9
548,377 + +1.0
548,378 - +0.5
548,464 + +0.6
548,465 - -1.7
548,465 - -0.4
548,465 - -0.3
548,489 + -0.7
548,502 - +0.5
548,537 + -1.2
548,538 - -0.3
548,548 + +2.1
548,549 - +3.1
548,549 - +1.2
548,829 - Pf6N2E2_482 0.37 -1.9
548,943 + Pf6N2E2_482 0.60 -2.6
548,943 + Pf6N2E2_482 0.60 -0.5
549,290 + -1.1
549,291 - +0.3
549,291 - +0.6
549,291 - -0.8
549,317 + +2.3
549,317 + -0.5
549,318 - -0.2

Or see this region's nucleotide sequence