Experiment: Zinc chloride 0.0000015625 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_46 and Pf6N2E2_47 overlap by 4 nucleotides Pf6N2E2_47 and Pf6N2E2_48 are separated by 89 nucleotides Pf6N2E2_48 and Pf6N2E2_6095 are separated by 251 nucleotides Pf6N2E2_6095 and Pf6N2E2_49 are separated by 235 nucleotides
Pf6N2E2_46 - membrane protein, putative, at 86,264 to 87,325
_46
Pf6N2E2_47 - FIG00961567: hypothetical protein, at 87,322 to 87,504
_47
Pf6N2E2_48 - FIG00955661: hypothetical protein, at 87,594 to 87,788
_48
Pf6N2E2_6095 - tRNA-Asn-GTT, at 88,040 to 88,112
_6095
Pf6N2E2_49 - FIG00967200: hypothetical protein, at 88,348 to 88,575
_49
Position (kb) 87
88 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 86.506 kb on + strand, within Pf6N2E2_46 at 86.506 kb on + strand, within Pf6N2E2_46 at 86.537 kb on + strand, within Pf6N2E2_46 at 86.537 kb on + strand, within Pf6N2E2_46 at 86.538 kb on - strand, within Pf6N2E2_46 at 86.538 kb on - strand, within Pf6N2E2_46 at 86.771 kb on + strand, within Pf6N2E2_46 at 86.771 kb on + strand, within Pf6N2E2_46 at 86.772 kb on - strand, within Pf6N2E2_46 at 86.772 kb on - strand, within Pf6N2E2_46 at 86.816 kb on - strand, within Pf6N2E2_46 at 87.059 kb on + strand, within Pf6N2E2_46 at 87.060 kb on - strand, within Pf6N2E2_46 at 87.185 kb on + strand, within Pf6N2E2_46 at 87.185 kb on + strand, within Pf6N2E2_46 at 87.185 kb on + strand, within Pf6N2E2_46 at 87.185 kb on + strand, within Pf6N2E2_46 at 87.185 kb on + strand, within Pf6N2E2_46 at 87.186 kb on - strand, within Pf6N2E2_46 at 87.186 kb on - strand, within Pf6N2E2_46 at 87.186 kb on - strand, within Pf6N2E2_46 at 87.323 kb on + strand at 87.338 kb on + strand at 87.339 kb on - strand at 87.531 kb on + strand at 87.531 kb on + strand at 87.536 kb on + strand at 87.536 kb on + strand at 87.536 kb on + strand at 87.575 kb on + strand at 87.575 kb on + strand at 87.575 kb on + strand at 87.576 kb on - strand at 87.578 kb on + strand at 87.579 kb on - strand at 87.733 kb on + strand, within Pf6N2E2_48 at 87.734 kb on - strand, within Pf6N2E2_48 at 87.734 kb on - strand, within Pf6N2E2_48 at 87.788 kb on + strand at 87.788 kb on + strand at 87.806 kb on + strand at 87.806 kb on + strand at 87.807 kb on - strand at 87.906 kb on + strand at 87.928 kb on + strand at 87.929 kb on - strand at 87.975 kb on + strand at 88.034 kb on - strand at 88.077 kb on - strand, within Pf6N2E2_6095 at 88.077 kb on - strand, within Pf6N2E2_6095 at 88.093 kb on - strand, within Pf6N2E2_6095 at 88.093 kb on - strand, within Pf6N2E2_6095 at 88.126 kb on + strand at 88.233 kb on + strand at 88.234 kb on - strand at 88.285 kb on + strand at 88.286 kb on - strand at 88.286 kb on - strand at 88.286 kb on - strand at 88.286 kb on - strand at 88.291 kb on - strand at 88.291 kb on - strand at 88.311 kb on + strand at 88.312 kb on - strand at 88.405 kb on + strand, within Pf6N2E2_49 at 88.406 kb on - strand, within Pf6N2E2_49 at 88.406 kb on - strand, within Pf6N2E2_49 at 88.491 kb on + strand, within Pf6N2E2_49
Per-strain Table
Position Strand Gene LocusTag Fraction Zinc chloride 0.0000015625 M remove 86,506 + Pf6N2E2_46 0.23 +1.0 86,506 + Pf6N2E2_46 0.23 +1.5 86,537 + Pf6N2E2_46 0.26 -0.3 86,537 + Pf6N2E2_46 0.26 +0.0 86,538 - Pf6N2E2_46 0.26 +1.0 86,538 - Pf6N2E2_46 0.26 +2.0 86,771 + Pf6N2E2_46 0.48 +0.1 86,771 + Pf6N2E2_46 0.48 -1.3 86,772 - Pf6N2E2_46 0.48 -1.7 86,772 - Pf6N2E2_46 0.48 -0.7 86,816 - Pf6N2E2_46 0.52 +0.3 87,059 + Pf6N2E2_46 0.75 -2.4 87,060 - Pf6N2E2_46 0.75 -0.8 87,185 + Pf6N2E2_46 0.87 +0.7 87,185 + Pf6N2E2_46 0.87 -2.0 87,185 + Pf6N2E2_46 0.87 +0.5 87,185 + Pf6N2E2_46 0.87 +0.8 87,185 + Pf6N2E2_46 0.87 +1.0 87,186 - Pf6N2E2_46 0.87 -1.2 87,186 - Pf6N2E2_46 0.87 -0.2 87,186 - Pf6N2E2_46 0.87 +0.3 87,323 + +0.1 87,338 + -2.2 87,339 - +2.9 87,531 + -0.1 87,531 + -1.4 87,536 + -2.3 87,536 + +0.8 87,536 + +0.0 87,575 + +0.4 87,575 + +0.2 87,575 + -2.1 87,576 - -0.0 87,578 + +0.8 87,579 - +1.5 87,733 + Pf6N2E2_48 0.71 -1.0 87,734 - Pf6N2E2_48 0.72 +2.0 87,734 - Pf6N2E2_48 0.72 +0.3 87,788 + -0.4 87,788 + +2.0 87,806 + +0.8 87,806 + -0.7 87,807 - -0.8 87,906 + +0.6 87,928 + +1.0 87,929 - +0.4 87,975 + +2.6 88,034 - +0.6 88,077 - Pf6N2E2_6095 0.51 -2.4 88,077 - Pf6N2E2_6095 0.51 -0.3 88,093 - Pf6N2E2_6095 0.73 -0.0 88,093 - Pf6N2E2_6095 0.73 +0.7 88,126 + -2.7 88,233 + -1.0 88,234 - +0.1 88,285 + +0.8 88,286 - +1.1 88,286 - +1.7 88,286 - +0.8 88,286 - +0.3 88,291 - -2.0 88,291 - -1.7 88,311 + -3.2 88,312 - +1.1 88,405 + Pf6N2E2_49 0.25 +1.2 88,406 - Pf6N2E2_49 0.25 -0.6 88,406 - Pf6N2E2_49 0.25 -1.1 88,491 + Pf6N2E2_49 0.63 -1.6
Or see this region's nucleotide sequence