Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_3344

Experiment: Zinc chloride 0.0000015625 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_3343 and Pf6N2E2_3344 overlap by 4 nucleotidesPf6N2E2_3344 and Pf6N2E2_3345 are separated by 64 nucleotides Pf6N2E2_3343 - AmpE protein, at 3,870,841 to 3,871,677 _3343 Pf6N2E2_3344 - N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28) AmpD, at 3,871,674 to 3,872,234 _3344 Pf6N2E2_3345 - Thymidine phosphorylase (EC 2.4.2.4), at 3,872,299 to 3,874,512 _3345 Position (kb) 3871 3872 3873Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3870.690 kb on + strandat 3870.690 kb on + strandat 3870.690 kb on + strandat 3870.690 kb on + strandat 3870.690 kb on + strandat 3870.691 kb on - strandat 3870.691 kb on - strandat 3870.691 kb on - strandat 3870.691 kb on - strandat 3870.875 kb on + strandat 3870.991 kb on - strand, within Pf6N2E2_3343at 3871.008 kb on + strand, within Pf6N2E2_3343at 3871.009 kb on - strand, within Pf6N2E2_3343at 3871.009 kb on - strand, within Pf6N2E2_3343at 3871.250 kb on + strand, within Pf6N2E2_3343at 3871.251 kb on - strand, within Pf6N2E2_3343at 3871.421 kb on + strand, within Pf6N2E2_3343at 3871.421 kb on + strand, within Pf6N2E2_3343at 3871.464 kb on - strand, within Pf6N2E2_3343at 3871.464 kb on - strand, within Pf6N2E2_3343at 3871.778 kb on + strand, within Pf6N2E2_3344at 3871.779 kb on - strand, within Pf6N2E2_3344at 3871.843 kb on + strand, within Pf6N2E2_3344at 3871.844 kb on - strand, within Pf6N2E2_3344at 3871.844 kb on - strand, within Pf6N2E2_3344at 3871.844 kb on - strand, within Pf6N2E2_3344at 3871.907 kb on + strand, within Pf6N2E2_3344at 3871.908 kb on - strand, within Pf6N2E2_3344at 3871.908 kb on - strand, within Pf6N2E2_3344at 3872.045 kb on - strand, within Pf6N2E2_3344at 3872.126 kb on + strand, within Pf6N2E2_3344at 3872.234 kb on + strandat 3872.235 kb on - strandat 3872.271 kb on + strandat 3872.272 kb on - strandat 3872.272 kb on - strandat 3872.465 kb on + strandat 3872.465 kb on + strandat 3872.465 kb on + strandat 3872.465 kb on + strandat 3872.466 kb on - strandat 3872.466 kb on - strandat 3872.466 kb on - strandat 3872.830 kb on + strand, within Pf6N2E2_3345at 3872.931 kb on - strand, within Pf6N2E2_3345at 3873.205 kb on + strand, within Pf6N2E2_3345

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Per-strain Table

Position Strand Gene LocusTag Fraction Zinc chloride 0.0000015625 M
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3,870,690 + -0.2
3,870,690 + -2.2
3,870,690 + +0.2
3,870,690 + -0.0
3,870,690 + -0.5
3,870,691 - -1.4
3,870,691 - -1.7
3,870,691 - -0.1
3,870,691 - -1.2
3,870,875 + -0.5
3,870,991 - Pf6N2E2_3343 0.18 -2.3
3,871,008 + Pf6N2E2_3343 0.20 -0.9
3,871,009 - Pf6N2E2_3343 0.20 +0.1
3,871,009 - Pf6N2E2_3343 0.20 -2.4
3,871,250 + Pf6N2E2_3343 0.49 -3.2
3,871,251 - Pf6N2E2_3343 0.49 -0.2
3,871,421 + Pf6N2E2_3343 0.69 -1.4
3,871,421 + Pf6N2E2_3343 0.69 +0.1
3,871,464 - Pf6N2E2_3343 0.74 +0.1
3,871,464 - Pf6N2E2_3343 0.74 -1.9
3,871,778 + Pf6N2E2_3344 0.19 -0.7
3,871,779 - Pf6N2E2_3344 0.19 -2.2
3,871,843 + Pf6N2E2_3344 0.30 +0.0
3,871,844 - Pf6N2E2_3344 0.30 +0.4
3,871,844 - Pf6N2E2_3344 0.30 +0.1
3,871,844 - Pf6N2E2_3344 0.30 -1.4
3,871,907 + Pf6N2E2_3344 0.42 -2.0
3,871,908 - Pf6N2E2_3344 0.42 -1.9
3,871,908 - Pf6N2E2_3344 0.42 -0.7
3,872,045 - Pf6N2E2_3344 0.66 -0.8
3,872,126 + Pf6N2E2_3344 0.81 -2.3
3,872,234 + +0.3
3,872,235 - +1.4
3,872,271 + +2.1
3,872,272 - +1.0
3,872,272 - +1.1
3,872,465 + -0.3
3,872,465 + -0.5
3,872,465 + -0.9
3,872,465 + -1.5
3,872,466 - +2.4
3,872,466 - +0.2
3,872,466 - +0.8
3,872,830 + Pf6N2E2_3345 0.24 -2.4
3,872,931 - Pf6N2E2_3345 0.29 +0.9
3,873,205 + Pf6N2E2_3345 0.41 +0.0

Or see this region's nucleotide sequence