Experiment: Zinc chloride 0.0000015625 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3191 and Pf6N2E2_3192 are separated by 1 nucleotides Pf6N2E2_3192 and Pf6N2E2_3193 are separated by 144 nucleotides
Pf6N2E2_3191 - Serine acetyltransferase (EC 2.3.1.30), at 3,684,848 to 3,685,624
_3191
Pf6N2E2_3192 - tRNA:Cm32/Um32 methyltransferase, at 3,685,626 to 3,686,396
_3192
Pf6N2E2_3193 - Inositol-1-monophosphatase (EC 3.1.3.25), at 3,686,541 to 3,687,359
_3193
Position (kb)
3685
3686
3687 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3684.697 kb on + strand at 3684.780 kb on + strand at 3684.780 kb on + strand at 3684.781 kb on - strand at 3684.781 kb on - strand at 3684.795 kb on + strand at 3684.849 kb on + strand at 3684.927 kb on + strand, within Pf6N2E2_3191 at 3684.928 kb on - strand, within Pf6N2E2_3191 at 3684.928 kb on - strand, within Pf6N2E2_3191 at 3684.931 kb on + strand, within Pf6N2E2_3191 at 3684.931 kb on + strand, within Pf6N2E2_3191 at 3684.932 kb on - strand, within Pf6N2E2_3191 at 3684.932 kb on - strand, within Pf6N2E2_3191 at 3684.932 kb on - strand, within Pf6N2E2_3191 at 3684.932 kb on - strand, within Pf6N2E2_3191 at 3685.054 kb on - strand, within Pf6N2E2_3191 at 3685.641 kb on - strand at 3685.693 kb on - strand at 3685.693 kb on - strand at 3685.694 kb on + strand at 3685.694 kb on + strand at 3685.694 kb on + strand at 3685.695 kb on - strand at 3685.695 kb on - strand at 3685.695 kb on - strand at 3685.695 kb on - strand at 3685.695 kb on - strand at 3685.695 kb on - strand at 3685.695 kb on - strand at 3685.695 kb on - strand at 3685.695 kb on - strand at 3685.695 kb on - strand at 3685.730 kb on - strand, within Pf6N2E2_3192 at 3685.816 kb on + strand, within Pf6N2E2_3192 at 3685.817 kb on - strand, within Pf6N2E2_3192 at 3685.817 kb on - strand, within Pf6N2E2_3192 at 3685.817 kb on - strand, within Pf6N2E2_3192 at 3685.933 kb on + strand, within Pf6N2E2_3192 at 3685.934 kb on - strand, within Pf6N2E2_3192 at 3685.934 kb on - strand, within Pf6N2E2_3192 at 3685.934 kb on - strand, within Pf6N2E2_3192 at 3686.141 kb on + strand, within Pf6N2E2_3192 at 3686.364 kb on - strand at 3686.418 kb on + strand at 3686.446 kb on + strand at 3686.446 kb on + strand at 3686.457 kb on + strand at 3687.358 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Zinc chloride 0.0000015625 M remove 3,684,697 + -0.8 3,684,780 + +2.0 3,684,780 + -0.6 3,684,781 - -0.8 3,684,781 - -0.1 3,684,795 + -1.7 3,684,849 + -0.9 3,684,927 + Pf6N2E2_3191 0.10 -1.2 3,684,928 - Pf6N2E2_3191 0.10 -1.5 3,684,928 - Pf6N2E2_3191 0.10 -1.1 3,684,931 + Pf6N2E2_3191 0.11 +0.6 3,684,931 + Pf6N2E2_3191 0.11 -1.3 3,684,932 - Pf6N2E2_3191 0.11 -1.7 3,684,932 - Pf6N2E2_3191 0.11 -0.9 3,684,932 - Pf6N2E2_3191 0.11 +1.0 3,684,932 - Pf6N2E2_3191 0.11 -0.4 3,685,054 - Pf6N2E2_3191 0.27 -2.5 3,685,641 - -1.4 3,685,693 - -0.9 3,685,693 - -2.3 3,685,694 + -0.8 3,685,694 + -1.2 3,685,694 + +1.2 3,685,695 - -3.2 3,685,695 - -1.5 3,685,695 - -1.5 3,685,695 - -2.5 3,685,695 - -2.6 3,685,695 - -3.0 3,685,695 - -2.9 3,685,695 - -3.0 3,685,695 - -0.2 3,685,695 - -3.0 3,685,730 - Pf6N2E2_3192 0.13 +0.3 3,685,816 + Pf6N2E2_3192 0.25 -1.2 3,685,817 - Pf6N2E2_3192 0.25 -0.9 3,685,817 - Pf6N2E2_3192 0.25 -0.9 3,685,817 - Pf6N2E2_3192 0.25 -1.9 3,685,933 + Pf6N2E2_3192 0.40 -0.7 3,685,934 - Pf6N2E2_3192 0.40 -1.6 3,685,934 - Pf6N2E2_3192 0.40 -2.7 3,685,934 - Pf6N2E2_3192 0.40 -2.3 3,686,141 + Pf6N2E2_3192 0.67 +0.0 3,686,364 - -1.3 3,686,418 + -1.7 3,686,446 + -2.0 3,686,446 + +1.2 3,686,457 + +1.6 3,687,358 - -2.1
Or see this region's nucleotide sequence