Experiment: Zinc chloride 0.0000015625 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2966 and Pf6N2E2_2967 are separated by 354 nucleotides Pf6N2E2_2967 and Pf6N2E2_2968 are separated by 31 nucleotides Pf6N2E2_2968 and Pf6N2E2_2969 are separated by 35 nucleotides Pf6N2E2_2969 and Pf6N2E2_2970 are separated by 157 nucleotides Pf6N2E2_2970 and Pf6N2E2_2971 are separated by 44 nucleotides
Pf6N2E2_2966 - Deoxycytidine triphosphate deaminase (EC 3.5.4.13), at 3,457,961 to 3,458,527
_2966
Pf6N2E2_2967 - Cold shock protein CspA, at 3,458,882 to 3,459,091
_2967
Pf6N2E2_2968 - hypothetical protein, at 3,459,123 to 3,459,251
_2968
Pf6N2E2_2969 - Peptide chain release factor RF-3, at 3,459,287 to 3,460,294
_2969
Pf6N2E2_2970 - methylated-DNA--protein-cysteine methyltransferase-related protein, at 3,460,452 to 3,460,805
_2970
Pf6N2E2_2971 - AmpG permease, at 3,460,850 to 3,462,409
_2971
Position (kb)
3459
3460
3461 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3458.575 kb on - strand at 3458.575 kb on - strand at 3458.678 kb on - strand at 3458.725 kb on - strand at 3458.772 kb on + strand at 3458.772 kb on + strand at 3458.772 kb on + strand at 3458.772 kb on + strand at 3458.772 kb on + strand at 3458.773 kb on - strand at 3458.773 kb on - strand at 3458.773 kb on - strand at 3458.797 kb on + strand at 3458.858 kb on + strand at 3458.858 kb on + strand at 3458.908 kb on + strand, within Pf6N2E2_2967 at 3458.908 kb on + strand, within Pf6N2E2_2967 at 3458.908 kb on + strand, within Pf6N2E2_2967 at 3458.908 kb on + strand, within Pf6N2E2_2967 at 3459.085 kb on + strand at 3459.089 kb on + strand at 3459.089 kb on + strand at 3459.089 kb on + strand at 3459.108 kb on + strand at 3459.430 kb on - strand, within Pf6N2E2_2969 at 3459.435 kb on + strand, within Pf6N2E2_2969 at 3459.436 kb on - strand, within Pf6N2E2_2969 at 3459.436 kb on - strand, within Pf6N2E2_2969 at 3459.516 kb on + strand, within Pf6N2E2_2969 at 3459.517 kb on - strand, within Pf6N2E2_2969 at 3459.517 kb on - strand, within Pf6N2E2_2969 at 3459.517 kb on - strand, within Pf6N2E2_2969 at 3459.517 kb on - strand, within Pf6N2E2_2969 at 3459.517 kb on - strand, within Pf6N2E2_2969 at 3459.541 kb on - strand, within Pf6N2E2_2969 at 3459.541 kb on - strand, within Pf6N2E2_2969 at 3459.541 kb on - strand, within Pf6N2E2_2969 at 3459.568 kb on - strand, within Pf6N2E2_2969 at 3459.573 kb on + strand, within Pf6N2E2_2969 at 3459.574 kb on - strand, within Pf6N2E2_2969 at 3459.696 kb on + strand, within Pf6N2E2_2969 at 3459.696 kb on + strand, within Pf6N2E2_2969 at 3459.697 kb on - strand, within Pf6N2E2_2969 at 3459.697 kb on - strand, within Pf6N2E2_2969 at 3459.804 kb on + strand, within Pf6N2E2_2969 at 3459.804 kb on + strand, within Pf6N2E2_2969 at 3459.804 kb on + strand, within Pf6N2E2_2969 at 3459.804 kb on + strand, within Pf6N2E2_2969 at 3459.804 kb on + strand, within Pf6N2E2_2969 at 3459.804 kb on + strand, within Pf6N2E2_2969 at 3459.805 kb on - strand, within Pf6N2E2_2969 at 3459.805 kb on - strand, within Pf6N2E2_2969 at 3459.821 kb on - strand, within Pf6N2E2_2969 at 3460.068 kb on + strand, within Pf6N2E2_2969 at 3460.068 kb on + strand, within Pf6N2E2_2969 at 3460.068 kb on + strand, within Pf6N2E2_2969 at 3460.069 kb on - strand, within Pf6N2E2_2969 at 3460.069 kb on - strand, within Pf6N2E2_2969 at 3460.069 kb on - strand, within Pf6N2E2_2969 at 3460.069 kb on - strand, within Pf6N2E2_2969 at 3460.069 kb on - strand, within Pf6N2E2_2969 at 3460.069 kb on - strand, within Pf6N2E2_2969 at 3460.069 kb on - strand, within Pf6N2E2_2969 at 3460.069 kb on - strand, within Pf6N2E2_2969 at 3460.069 kb on - strand, within Pf6N2E2_2969 at 3460.069 kb on - strand, within Pf6N2E2_2969 at 3460.107 kb on - strand, within Pf6N2E2_2969 at 3460.149 kb on + strand, within Pf6N2E2_2969 at 3460.286 kb on - strand at 3460.294 kb on + strand at 3460.294 kb on + strand at 3460.295 kb on - strand at 3460.295 kb on - strand at 3460.295 kb on - strand at 3460.295 kb on - strand at 3460.408 kb on + strand at 3460.409 kb on - strand at 3460.415 kb on + strand at 3460.415 kb on + strand at 3460.415 kb on + strand at 3460.415 kb on + strand at 3460.416 kb on - strand at 3460.416 kb on - strand at 3460.420 kb on + strand at 3460.420 kb on + strand at 3460.420 kb on + strand at 3460.449 kb on + strand at 3460.449 kb on + strand at 3460.450 kb on - strand at 3460.450 kb on - strand at 3460.510 kb on - strand, within Pf6N2E2_2970 at 3460.510 kb on - strand, within Pf6N2E2_2970 at 3460.763 kb on + strand, within Pf6N2E2_2970 at 3460.763 kb on + strand, within Pf6N2E2_2970 at 3460.764 kb on - strand, within Pf6N2E2_2970 at 3460.859 kb on - strand at 3460.887 kb on - strand at 3460.943 kb on + strand at 3460.943 kb on + strand at 3460.943 kb on + strand at 3460.943 kb on + strand at 3460.944 kb on - strand at 3460.944 kb on - strand at 3460.944 kb on - strand at 3460.944 kb on - strand at 3460.950 kb on + strand at 3460.951 kb on - strand at 3460.951 kb on - strand at 3460.951 kb on - strand at 3460.951 kb on - strand at 3460.951 kb on - strand at 3461.036 kb on + strand, within Pf6N2E2_2971 at 3461.036 kb on + strand, within Pf6N2E2_2971 at 3461.036 kb on + strand, within Pf6N2E2_2971 at 3461.037 kb on - strand, within Pf6N2E2_2971 at 3461.037 kb on - strand, within Pf6N2E2_2971 at 3461.037 kb on - strand, within Pf6N2E2_2971 at 3461.044 kb on + strand, within Pf6N2E2_2971 at 3461.044 kb on + strand, within Pf6N2E2_2971 at 3461.044 kb on + strand, within Pf6N2E2_2971 at 3461.044 kb on + strand, within Pf6N2E2_2971 at 3461.044 kb on + strand, within Pf6N2E2_2971 at 3461.044 kb on + strand, within Pf6N2E2_2971 at 3461.044 kb on + strand, within Pf6N2E2_2971 at 3461.044 kb on + strand, within Pf6N2E2_2971 at 3461.045 kb on - strand, within Pf6N2E2_2971 at 3461.142 kb on + strand, within Pf6N2E2_2971 at 3461.142 kb on + strand, within Pf6N2E2_2971 at 3461.143 kb on - strand, within Pf6N2E2_2971 at 3461.143 kb on - strand, within Pf6N2E2_2971
Per-strain Table
Position Strand Gene LocusTag Fraction Zinc chloride 0.0000015625 M remove 3,458,575 - +0.2 3,458,575 - -0.4 3,458,678 - +0.0 3,458,725 - -1.8 3,458,772 + -1.6 3,458,772 + +0.8 3,458,772 + +0.6 3,458,772 + -0.4 3,458,772 + +0.0 3,458,773 - -0.5 3,458,773 - +1.1 3,458,773 - -1.2 3,458,797 + +1.8 3,458,858 + -0.8 3,458,858 + +0.6 3,458,908 + Pf6N2E2_2967 0.12 -0.8 3,458,908 + Pf6N2E2_2967 0.12 +1.5 3,458,908 + Pf6N2E2_2967 0.12 -2.1 3,458,908 + Pf6N2E2_2967 0.12 +0.0 3,459,085 + -0.3 3,459,089 + -1.3 3,459,089 + -2.6 3,459,089 + +0.5 3,459,108 + +3.0 3,459,430 - Pf6N2E2_2969 0.14 +1.7 3,459,435 + Pf6N2E2_2969 0.15 +1.9 3,459,436 - Pf6N2E2_2969 0.15 -1.8 3,459,436 - Pf6N2E2_2969 0.15 +0.1 3,459,516 + Pf6N2E2_2969 0.23 +0.7 3,459,517 - Pf6N2E2_2969 0.23 +1.7 3,459,517 - Pf6N2E2_2969 0.23 +1.5 3,459,517 - Pf6N2E2_2969 0.23 +0.2 3,459,517 - Pf6N2E2_2969 0.23 +0.7 3,459,517 - Pf6N2E2_2969 0.23 -0.3 3,459,541 - Pf6N2E2_2969 0.25 +1.8 3,459,541 - Pf6N2E2_2969 0.25 +0.5 3,459,541 - Pf6N2E2_2969 0.25 +1.0 3,459,568 - Pf6N2E2_2969 0.28 +0.3 3,459,573 + Pf6N2E2_2969 0.28 +2.4 3,459,574 - Pf6N2E2_2969 0.28 +1.1 3,459,696 + Pf6N2E2_2969 0.41 +1.5 3,459,696 + Pf6N2E2_2969 0.41 +0.8 3,459,697 - Pf6N2E2_2969 0.41 +0.6 3,459,697 - Pf6N2E2_2969 0.41 +2.3 3,459,804 + Pf6N2E2_2969 0.51 +0.2 3,459,804 + Pf6N2E2_2969 0.51 -0.8 3,459,804 + Pf6N2E2_2969 0.51 +1.3 3,459,804 + Pf6N2E2_2969 0.51 +1.2 3,459,804 + Pf6N2E2_2969 0.51 +1.9 3,459,804 + Pf6N2E2_2969 0.51 -1.6 3,459,805 - Pf6N2E2_2969 0.51 +3.2 3,459,805 - Pf6N2E2_2969 0.51 -1.2 3,459,821 - Pf6N2E2_2969 0.53 +0.3 3,460,068 + Pf6N2E2_2969 0.77 -2.3 3,460,068 + Pf6N2E2_2969 0.77 +1.3 3,460,068 + Pf6N2E2_2969 0.77 +2.4 3,460,069 - Pf6N2E2_2969 0.78 +2.2 3,460,069 - Pf6N2E2_2969 0.78 +0.5 3,460,069 - Pf6N2E2_2969 0.78 -0.2 3,460,069 - Pf6N2E2_2969 0.78 +2.5 3,460,069 - Pf6N2E2_2969 0.78 +3.0 3,460,069 - Pf6N2E2_2969 0.78 +1.8 3,460,069 - Pf6N2E2_2969 0.78 +3.2 3,460,069 - Pf6N2E2_2969 0.78 -0.5 3,460,069 - Pf6N2E2_2969 0.78 +0.4 3,460,069 - Pf6N2E2_2969 0.78 -0.4 3,460,107 - Pf6N2E2_2969 0.81 +3.9 3,460,149 + Pf6N2E2_2969 0.86 +2.2 3,460,286 - -2.0 3,460,294 + -0.7 3,460,294 + +1.3 3,460,295 - -0.7 3,460,295 - +1.5 3,460,295 - -1.9 3,460,295 - -0.9 3,460,408 + +0.6 3,460,409 - +1.4 3,460,415 + -1.9 3,460,415 + +0.0 3,460,415 + +1.4 3,460,415 + +1.0 3,460,416 - +0.7 3,460,416 - -1.3 3,460,420 + +1.6 3,460,420 + -0.9 3,460,420 + +1.1 3,460,449 + +0.6 3,460,449 + +0.4 3,460,450 - +0.2 3,460,450 - +1.1 3,460,510 - Pf6N2E2_2970 0.16 +1.4 3,460,510 - Pf6N2E2_2970 0.16 +1.7 3,460,763 + Pf6N2E2_2970 0.88 -0.6 3,460,763 + Pf6N2E2_2970 0.88 +0.6 3,460,764 - Pf6N2E2_2970 0.88 -0.1 3,460,859 - -2.2 3,460,887 - -2.5 3,460,943 + -2.9 3,460,943 + -3.0 3,460,943 + -2.7 3,460,943 + -2.3 3,460,944 - +1.2 3,460,944 - -2.8 3,460,944 - -1.7 3,460,944 - -3.5 3,460,950 + -1.8 3,460,951 - -1.5 3,460,951 - -1.9 3,460,951 - -1.2 3,460,951 - -3.2 3,460,951 - -3.0 3,461,036 + Pf6N2E2_2971 0.12 -2.4 3,461,036 + Pf6N2E2_2971 0.12 -2.8 3,461,036 + Pf6N2E2_2971 0.12 -2.9 3,461,037 - Pf6N2E2_2971 0.12 -2.1 3,461,037 - Pf6N2E2_2971 0.12 -3.0 3,461,037 - Pf6N2E2_2971 0.12 -1.3 3,461,044 + Pf6N2E2_2971 0.12 -2.0 3,461,044 + Pf6N2E2_2971 0.12 -3.1 3,461,044 + Pf6N2E2_2971 0.12 -2.4 3,461,044 + Pf6N2E2_2971 0.12 -2.9 3,461,044 + Pf6N2E2_2971 0.12 -2.1 3,461,044 + Pf6N2E2_2971 0.12 -2.1 3,461,044 + Pf6N2E2_2971 0.12 -2.5 3,461,044 + Pf6N2E2_2971 0.12 -0.4 3,461,045 - Pf6N2E2_2971 0.12 -1.6 3,461,142 + Pf6N2E2_2971 0.19 -1.4 3,461,142 + Pf6N2E2_2971 0.19 -3.1 3,461,143 - Pf6N2E2_2971 0.19 -0.6 3,461,143 - Pf6N2E2_2971 0.19 -2.8
Or see this region's nucleotide sequence