Experiment: Zinc chloride 0.0000015625 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2372 and Pf6N2E2_2373 are separated by 4 nucleotides Pf6N2E2_2373 and Pf6N2E2_2374 are separated by 243 nucleotides
Pf6N2E2_2372 - Type III secretion outermembrane pore forming protein (YscC,MxiD,HrcC, InvG), at 2,806,770 to 2,808,425
_2372
Pf6N2E2_2373 - Type III secretion thermoregulatory protein (LcrF,VirF,transcription regulation of virulence plasmid), at 2,808,430 to 2,809,167
_2373
Pf6N2E2_2374 - Nitrate ABC transporter, nitrate-binding protein, at 2,809,411 to 2,810,622
_2374
Position (kb)
2808
2809
2810 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2807.852 kb on - strand, within Pf6N2E2_2372 at 2807.971 kb on + strand, within Pf6N2E2_2372 at 2807.971 kb on + strand, within Pf6N2E2_2372 at 2807.972 kb on - strand, within Pf6N2E2_2372 at 2807.972 kb on - strand, within Pf6N2E2_2372 at 2807.972 kb on - strand, within Pf6N2E2_2372 at 2808.128 kb on - strand, within Pf6N2E2_2372 at 2808.128 kb on - strand, within Pf6N2E2_2372 at 2808.128 kb on - strand, within Pf6N2E2_2372 at 2808.133 kb on + strand, within Pf6N2E2_2372 at 2808.133 kb on + strand, within Pf6N2E2_2372 at 2808.133 kb on + strand, within Pf6N2E2_2372 at 2808.133 kb on + strand, within Pf6N2E2_2372 at 2808.133 kb on + strand, within Pf6N2E2_2372 at 2808.134 kb on - strand, within Pf6N2E2_2372 at 2808.134 kb on - strand, within Pf6N2E2_2372 at 2808.134 kb on - strand, within Pf6N2E2_2372 at 2808.154 kb on + strand, within Pf6N2E2_2372 at 2808.334 kb on + strand at 2808.334 kb on + strand at 2808.334 kb on + strand at 2808.334 kb on + strand at 2808.335 kb on - strand at 2808.377 kb on + strand at 2808.447 kb on - strand at 2808.447 kb on - strand at 2808.447 kb on - strand at 2808.447 kb on - strand at 2808.727 kb on + strand, within Pf6N2E2_2373 at 2808.736 kb on + strand, within Pf6N2E2_2373 at 2808.786 kb on - strand, within Pf6N2E2_2373 at 2809.183 kb on + strand at 2809.183 kb on + strand at 2809.184 kb on - strand at 2809.184 kb on - strand at 2809.187 kb on + strand at 2809.187 kb on + strand at 2809.187 kb on + strand at 2809.187 kb on + strand at 2809.187 kb on + strand at 2809.188 kb on - strand at 2809.265 kb on + strand at 2809.265 kb on + strand at 2809.265 kb on + strand at 2809.266 kb on - strand at 2809.266 kb on - strand at 2809.266 kb on - strand at 2809.331 kb on - strand at 2809.657 kb on + strand, within Pf6N2E2_2374 at 2809.657 kb on + strand, within Pf6N2E2_2374 at 2809.658 kb on - strand, within Pf6N2E2_2374 at 2809.658 kb on - strand, within Pf6N2E2_2374 at 2809.870 kb on + strand, within Pf6N2E2_2374 at 2809.870 kb on + strand, within Pf6N2E2_2374 at 2809.870 kb on + strand, within Pf6N2E2_2374 at 2809.870 kb on + strand, within Pf6N2E2_2374 at 2809.870 kb on + strand, within Pf6N2E2_2374 at 2809.870 kb on + strand, within Pf6N2E2_2374 at 2809.871 kb on - strand, within Pf6N2E2_2374 at 2809.871 kb on - strand, within Pf6N2E2_2374 at 2809.871 kb on - strand, within Pf6N2E2_2374 at 2809.871 kb on - strand, within Pf6N2E2_2374 at 2809.873 kb on + strand, within Pf6N2E2_2374 at 2809.874 kb on - strand, within Pf6N2E2_2374 at 2809.969 kb on - strand, within Pf6N2E2_2374
Per-strain Table
Position Strand Gene LocusTag Fraction Zinc chloride 0.0000015625 M remove 2,807,852 - Pf6N2E2_2372 0.65 -1.3 2,807,971 + Pf6N2E2_2372 0.73 -0.1 2,807,971 + Pf6N2E2_2372 0.73 +0.7 2,807,972 - Pf6N2E2_2372 0.73 +0.7 2,807,972 - Pf6N2E2_2372 0.73 -1.8 2,807,972 - Pf6N2E2_2372 0.73 +0.7 2,808,128 - Pf6N2E2_2372 0.82 +0.8 2,808,128 - Pf6N2E2_2372 0.82 -2.1 2,808,128 - Pf6N2E2_2372 0.82 -1.1 2,808,133 + Pf6N2E2_2372 0.82 -0.4 2,808,133 + Pf6N2E2_2372 0.82 +1.0 2,808,133 + Pf6N2E2_2372 0.82 +0.2 2,808,133 + Pf6N2E2_2372 0.82 -2.2 2,808,133 + Pf6N2E2_2372 0.82 +1.2 2,808,134 - Pf6N2E2_2372 0.82 -2.6 2,808,134 - Pf6N2E2_2372 0.82 -0.8 2,808,134 - Pf6N2E2_2372 0.82 +0.3 2,808,154 + Pf6N2E2_2372 0.84 -0.4 2,808,334 + -1.1 2,808,334 + +0.3 2,808,334 + +0.4 2,808,334 + +1.5 2,808,335 - +1.4 2,808,377 + +1.8 2,808,447 - -0.6 2,808,447 - +0.3 2,808,447 - +0.8 2,808,447 - -1.3 2,808,727 + Pf6N2E2_2373 0.40 -3.0 2,808,736 + Pf6N2E2_2373 0.41 -0.8 2,808,786 - Pf6N2E2_2373 0.48 -0.9 2,809,183 + -0.3 2,809,183 + +0.6 2,809,184 - +0.2 2,809,184 - -1.7 2,809,187 + +0.9 2,809,187 + -3.2 2,809,187 + +1.5 2,809,187 + +0.7 2,809,187 + -0.9 2,809,188 - -2.4 2,809,265 + +1.7 2,809,265 + -0.8 2,809,265 + -1.9 2,809,266 - -2.2 2,809,266 - +1.6 2,809,266 - +0.1 2,809,331 - -2.6 2,809,657 + Pf6N2E2_2374 0.20 +0.9 2,809,657 + Pf6N2E2_2374 0.20 -2.4 2,809,658 - Pf6N2E2_2374 0.20 +0.1 2,809,658 - Pf6N2E2_2374 0.20 -0.8 2,809,870 + Pf6N2E2_2374 0.38 +1.3 2,809,870 + Pf6N2E2_2374 0.38 +1.2 2,809,870 + Pf6N2E2_2374 0.38 -0.5 2,809,870 + Pf6N2E2_2374 0.38 +1.3 2,809,870 + Pf6N2E2_2374 0.38 +0.9 2,809,870 + Pf6N2E2_2374 0.38 -0.7 2,809,871 - Pf6N2E2_2374 0.38 -1.0 2,809,871 - Pf6N2E2_2374 0.38 +0.9 2,809,871 - Pf6N2E2_2374 0.38 -0.8 2,809,871 - Pf6N2E2_2374 0.38 -1.2 2,809,873 + Pf6N2E2_2374 0.38 +0.3 2,809,874 - Pf6N2E2_2374 0.38 +2.1 2,809,969 - Pf6N2E2_2374 0.46 +0.4
Or see this region's nucleotide sequence