Experiment: Zinc chloride 0.0000015625 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1957 and Pf6N2E2_1958 are separated by 170 nucleotides Pf6N2E2_1958 and Pf6N2E2_1959 are separated by 188 nucleotides
Pf6N2E2_1957 - putative inner membrane protein, at 2,321,749 to 2,323,065
_1957
Pf6N2E2_1958 - Predicted dinucleotide-binding enzymes, at 2,323,236 to 2,323,778
_1958
Pf6N2E2_1959 - D-sorbitol dehydrogenase (EC 1.1.1.14) (from data) , at 2,323,967 to 2,324,740
_1959
Position (kb)
2323
2324 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2322.344 kb on + strand, within Pf6N2E2_1957 at 2322.451 kb on + strand, within Pf6N2E2_1957 at 2322.484 kb on + strand, within Pf6N2E2_1957 at 2322.508 kb on + strand, within Pf6N2E2_1957 at 2322.508 kb on + strand, within Pf6N2E2_1957 at 2322.611 kb on + strand, within Pf6N2E2_1957 at 2322.807 kb on + strand, within Pf6N2E2_1957 at 2322.966 kb on + strand at 2322.966 kb on + strand at 2322.967 kb on - strand at 2322.967 kb on - strand at 2322.967 kb on - strand at 2323.029 kb on + strand at 2323.071 kb on - strand at 2323.201 kb on + strand at 2323.201 kb on + strand at 2323.202 kb on - strand at 2323.202 kb on - strand at 2323.459 kb on + strand, within Pf6N2E2_1958 at 2323.459 kb on + strand, within Pf6N2E2_1958 at 2323.459 kb on + strand, within Pf6N2E2_1958 at 2323.459 kb on + strand, within Pf6N2E2_1958 at 2323.462 kb on - strand, within Pf6N2E2_1958 at 2323.507 kb on - strand, within Pf6N2E2_1958 at 2323.777 kb on - strand at 2323.777 kb on - strand at 2323.800 kb on + strand at 2323.804 kb on + strand at 2323.805 kb on - strand at 2323.805 kb on - strand at 2323.805 kb on - strand at 2323.824 kb on + strand at 2323.999 kb on - strand at 2324.016 kb on + strand at 2324.123 kb on + strand, within Pf6N2E2_1959 at 2324.251 kb on + strand, within Pf6N2E2_1959 at 2324.578 kb on - strand, within Pf6N2E2_1959 at 2324.637 kb on - strand, within Pf6N2E2_1959 at 2324.664 kb on + strand at 2324.664 kb on + strand at 2324.664 kb on + strand at 2324.665 kb on - strand at 2324.665 kb on - strand at 2324.665 kb on - strand at 2324.665 kb on - strand at 2324.722 kb on + strand at 2324.748 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Zinc chloride 0.0000015625 M remove 2,322,344 + Pf6N2E2_1957 0.45 -1.0 2,322,451 + Pf6N2E2_1957 0.53 +0.3 2,322,484 + Pf6N2E2_1957 0.56 +0.4 2,322,508 + Pf6N2E2_1957 0.58 +1.1 2,322,508 + Pf6N2E2_1957 0.58 -1.0 2,322,611 + Pf6N2E2_1957 0.65 -0.1 2,322,807 + Pf6N2E2_1957 0.80 +0.5 2,322,966 + -2.0 2,322,966 + +1.4 2,322,967 - +0.2 2,322,967 - +2.6 2,322,967 - +2.4 2,323,029 + -0.0 2,323,071 - +1.2 2,323,201 + -1.5 2,323,201 + +0.4 2,323,202 - +0.2 2,323,202 - -2.7 2,323,459 + Pf6N2E2_1958 0.41 +2.0 2,323,459 + Pf6N2E2_1958 0.41 -2.6 2,323,459 + Pf6N2E2_1958 0.41 +1.6 2,323,459 + Pf6N2E2_1958 0.41 +1.3 2,323,462 - Pf6N2E2_1958 0.42 +1.6 2,323,507 - Pf6N2E2_1958 0.50 -3.5 2,323,777 - +2.0 2,323,777 - -0.9 2,323,800 + -0.2 2,323,804 + +2.1 2,323,805 - +1.8 2,323,805 - -0.0 2,323,805 - -2.2 2,323,824 + -0.5 2,323,999 - +0.2 2,324,016 + +0.8 2,324,123 + Pf6N2E2_1959 0.20 +0.6 2,324,251 + Pf6N2E2_1959 0.37 +0.4 2,324,578 - Pf6N2E2_1959 0.79 -0.7 2,324,637 - Pf6N2E2_1959 0.87 -3.3 2,324,664 + -0.5 2,324,664 + -0.9 2,324,664 + +0.8 2,324,665 - -0.8 2,324,665 - +0.5 2,324,665 - +1.3 2,324,665 - +1.8 2,324,722 + +0.4 2,324,748 - -0.5
Or see this region's nucleotide sequence