Experiment: Zinc chloride 0.0000015625 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1952 and Pf6N2E2_1953 are separated by 100 nucleotides Pf6N2E2_1953 and Pf6N2E2_1954 are separated by 160 nucleotides
Pf6N2E2_1952 - Transcriptional regulator, LysR family, at 2,317,075 to 2,317,980
_1952
Pf6N2E2_1953 - Uncharacterized conserved protein, at 2,318,081 to 2,318,434
_1953
Pf6N2E2_1954 - Transcriptional regulator, LysR family, at 2,318,595 to 2,319,482
_1954
Position (kb)
2318
2319 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2317.143 kb on - strand at 2317.154 kb on + strand at 2317.155 kb on - strand at 2317.155 kb on - strand at 2317.155 kb on - strand at 2317.155 kb on - strand at 2317.155 kb on - strand at 2317.155 kb on - strand at 2317.159 kb on - strand at 2317.159 kb on - strand at 2317.159 kb on - strand at 2317.159 kb on - strand at 2317.205 kb on - strand, within Pf6N2E2_1952 at 2317.205 kb on - strand, within Pf6N2E2_1952 at 2317.220 kb on - strand, within Pf6N2E2_1952 at 2317.251 kb on - strand, within Pf6N2E2_1952 at 2317.251 kb on - strand, within Pf6N2E2_1952 at 2317.251 kb on - strand, within Pf6N2E2_1952 at 2317.251 kb on - strand, within Pf6N2E2_1952 at 2317.334 kb on - strand, within Pf6N2E2_1952 at 2317.367 kb on + strand, within Pf6N2E2_1952 at 2317.367 kb on + strand, within Pf6N2E2_1952 at 2317.368 kb on - strand, within Pf6N2E2_1952 at 2317.368 kb on - strand, within Pf6N2E2_1952 at 2317.368 kb on - strand, within Pf6N2E2_1952 at 2317.454 kb on + strand, within Pf6N2E2_1952 at 2317.469 kb on - strand, within Pf6N2E2_1952 at 2317.522 kb on + strand, within Pf6N2E2_1952 at 2317.523 kb on - strand, within Pf6N2E2_1952 at 2317.579 kb on - strand, within Pf6N2E2_1952 at 2317.579 kb on - strand, within Pf6N2E2_1952 at 2317.579 kb on - strand, within Pf6N2E2_1952 at 2317.690 kb on - strand, within Pf6N2E2_1952 at 2317.717 kb on - strand, within Pf6N2E2_1952 at 2317.717 kb on - strand, within Pf6N2E2_1952 at 2317.717 kb on - strand, within Pf6N2E2_1952 at 2317.885 kb on + strand, within Pf6N2E2_1952 at 2317.885 kb on + strand, within Pf6N2E2_1952 at 2317.885 kb on + strand, within Pf6N2E2_1952 at 2317.885 kb on + strand, within Pf6N2E2_1952 at 2318.007 kb on + strand at 2318.007 kb on + strand at 2318.008 kb on - strand at 2318.008 kb on - strand at 2318.132 kb on + strand, within Pf6N2E2_1953 at 2318.132 kb on + strand, within Pf6N2E2_1953 at 2318.132 kb on + strand, within Pf6N2E2_1953 at 2318.132 kb on + strand, within Pf6N2E2_1953 at 2318.132 kb on + strand, within Pf6N2E2_1953 at 2318.133 kb on - strand, within Pf6N2E2_1953 at 2318.133 kb on - strand, within Pf6N2E2_1953 at 2318.194 kb on + strand, within Pf6N2E2_1953 at 2318.226 kb on + strand, within Pf6N2E2_1953 at 2318.226 kb on + strand, within Pf6N2E2_1953 at 2318.227 kb on - strand, within Pf6N2E2_1953 at 2318.227 kb on - strand, within Pf6N2E2_1953 at 2318.227 kb on - strand, within Pf6N2E2_1953 at 2318.366 kb on + strand, within Pf6N2E2_1953 at 2318.434 kb on + strand at 2318.555 kb on + strand at 2318.583 kb on + strand at 2318.656 kb on + strand at 2318.659 kb on + strand at 2318.660 kb on - strand at 2318.755 kb on + strand, within Pf6N2E2_1954 at 2318.755 kb on + strand, within Pf6N2E2_1954 at 2318.767 kb on - strand, within Pf6N2E2_1954 at 2318.873 kb on - strand, within Pf6N2E2_1954 at 2318.873 kb on - strand, within Pf6N2E2_1954 at 2318.906 kb on + strand, within Pf6N2E2_1954 at 2318.906 kb on + strand, within Pf6N2E2_1954 at 2318.907 kb on - strand, within Pf6N2E2_1954 at 2318.920 kb on - strand, within Pf6N2E2_1954 at 2319.091 kb on + strand, within Pf6N2E2_1954 at 2319.189 kb on - strand, within Pf6N2E2_1954 at 2319.191 kb on + strand, within Pf6N2E2_1954 at 2319.192 kb on - strand, within Pf6N2E2_1954 at 2319.279 kb on + strand, within Pf6N2E2_1954 at 2319.297 kb on + strand, within Pf6N2E2_1954 at 2319.302 kb on + strand, within Pf6N2E2_1954 at 2319.302 kb on + strand, within Pf6N2E2_1954 at 2319.303 kb on - strand, within Pf6N2E2_1954 at 2319.303 kb on - strand, within Pf6N2E2_1954 at 2319.303 kb on - strand, within Pf6N2E2_1954 at 2319.309 kb on + strand, within Pf6N2E2_1954 at 2319.310 kb on - strand, within Pf6N2E2_1954 at 2319.330 kb on - strand, within Pf6N2E2_1954 at 2319.399 kb on + strand at 2319.399 kb on + strand at 2319.403 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Zinc chloride 0.0000015625 M remove 2,317,143 - -1.6 2,317,154 + +3.0 2,317,155 - +1.3 2,317,155 - +2.0 2,317,155 - -0.4 2,317,155 - -0.0 2,317,155 - -0.5 2,317,155 - -3.0 2,317,159 - +1.0 2,317,159 - +1.0 2,317,159 - -1.0 2,317,159 - +1.2 2,317,205 - Pf6N2E2_1952 0.14 -0.6 2,317,205 - Pf6N2E2_1952 0.14 +0.1 2,317,220 - Pf6N2E2_1952 0.16 -1.4 2,317,251 - Pf6N2E2_1952 0.19 -1.5 2,317,251 - Pf6N2E2_1952 0.19 -0.9 2,317,251 - Pf6N2E2_1952 0.19 -0.8 2,317,251 - Pf6N2E2_1952 0.19 -0.6 2,317,334 - Pf6N2E2_1952 0.29 -0.8 2,317,367 + Pf6N2E2_1952 0.32 +0.5 2,317,367 + Pf6N2E2_1952 0.32 +0.1 2,317,368 - Pf6N2E2_1952 0.32 +1.9 2,317,368 - Pf6N2E2_1952 0.32 +1.5 2,317,368 - Pf6N2E2_1952 0.32 -1.1 2,317,454 + Pf6N2E2_1952 0.42 -1.5 2,317,469 - Pf6N2E2_1952 0.43 -1.4 2,317,522 + Pf6N2E2_1952 0.49 +1.0 2,317,523 - Pf6N2E2_1952 0.49 -0.0 2,317,579 - Pf6N2E2_1952 0.56 +1.3 2,317,579 - Pf6N2E2_1952 0.56 +1.7 2,317,579 - Pf6N2E2_1952 0.56 -0.3 2,317,690 - Pf6N2E2_1952 0.68 +0.5 2,317,717 - Pf6N2E2_1952 0.71 +2.9 2,317,717 - Pf6N2E2_1952 0.71 -0.2 2,317,717 - Pf6N2E2_1952 0.71 +0.7 2,317,885 + Pf6N2E2_1952 0.89 -0.9 2,317,885 + Pf6N2E2_1952 0.89 -0.2 2,317,885 + Pf6N2E2_1952 0.89 -0.8 2,317,885 + Pf6N2E2_1952 0.89 +0.5 2,318,007 + -1.8 2,318,007 + -2.0 2,318,008 - +1.3 2,318,008 - -0.2 2,318,132 + Pf6N2E2_1953 0.14 +1.4 2,318,132 + Pf6N2E2_1953 0.14 +1.2 2,318,132 + Pf6N2E2_1953 0.14 +1.6 2,318,132 + Pf6N2E2_1953 0.14 -0.2 2,318,132 + Pf6N2E2_1953 0.14 +2.8 2,318,133 - Pf6N2E2_1953 0.15 +1.0 2,318,133 - Pf6N2E2_1953 0.15 +0.7 2,318,194 + Pf6N2E2_1953 0.32 +1.2 2,318,226 + Pf6N2E2_1953 0.41 -1.8 2,318,226 + Pf6N2E2_1953 0.41 -2.1 2,318,227 - Pf6N2E2_1953 0.41 -1.2 2,318,227 - Pf6N2E2_1953 0.41 -1.8 2,318,227 - Pf6N2E2_1953 0.41 +0.1 2,318,366 + Pf6N2E2_1953 0.81 -0.9 2,318,434 + -2.5 2,318,555 + +0.5 2,318,583 + -2.0 2,318,656 + +0.5 2,318,659 + +2.6 2,318,660 - -0.4 2,318,755 + Pf6N2E2_1954 0.18 -0.9 2,318,755 + Pf6N2E2_1954 0.18 -1.3 2,318,767 - Pf6N2E2_1954 0.19 -0.3 2,318,873 - Pf6N2E2_1954 0.31 -0.0 2,318,873 - Pf6N2E2_1954 0.31 -0.6 2,318,906 + Pf6N2E2_1954 0.35 +0.6 2,318,906 + Pf6N2E2_1954 0.35 +0.9 2,318,907 - Pf6N2E2_1954 0.35 +0.8 2,318,920 - Pf6N2E2_1954 0.37 -0.2 2,319,091 + Pf6N2E2_1954 0.56 -1.3 2,319,189 - Pf6N2E2_1954 0.67 -1.4 2,319,191 + Pf6N2E2_1954 0.67 +0.7 2,319,192 - Pf6N2E2_1954 0.67 -0.0 2,319,279 + Pf6N2E2_1954 0.77 -1.4 2,319,297 + Pf6N2E2_1954 0.79 +0.1 2,319,302 + Pf6N2E2_1954 0.80 -1.7 2,319,302 + Pf6N2E2_1954 0.80 -0.0 2,319,303 - Pf6N2E2_1954 0.80 -0.9 2,319,303 - Pf6N2E2_1954 0.80 +0.3 2,319,303 - Pf6N2E2_1954 0.80 +0.3 2,319,309 + Pf6N2E2_1954 0.80 +1.2 2,319,310 - Pf6N2E2_1954 0.81 +0.6 2,319,330 - Pf6N2E2_1954 0.83 -1.5 2,319,399 + -0.4 2,319,399 + +1.3 2,319,403 - -1.9
Or see this region's nucleotide sequence