Experiment: Zinc chloride 0.0000015625 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1484 and Pf6N2E2_1485 are separated by 113 nucleotides Pf6N2E2_1485 and Pf6N2E2_1486 are separated by 147 nucleotides
Pf6N2E2_1484 - N-acetyl-gamma-glutamyl-phosphate reductase (EC 1.2.1.38), at 1,733,849 to 1,734,778
_1484
Pf6N2E2_1485 - Transcriptional regulator, at 1,734,892 to 1,735,776
_1485
Pf6N2E2_1486 - Multidrug resistance ABC transporter ATP-binding and permease protein, at 1,735,924 to 1,737,783
_1486
Position (kb)
1734
1735
1736 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1733.957 kb on - strand, within Pf6N2E2_1484 at 1734.050 kb on + strand, within Pf6N2E2_1484 at 1734.050 kb on + strand, within Pf6N2E2_1484 at 1734.050 kb on + strand, within Pf6N2E2_1484 at 1734.050 kb on + strand, within Pf6N2E2_1484 at 1734.051 kb on - strand, within Pf6N2E2_1484 at 1734.051 kb on - strand, within Pf6N2E2_1484 at 1734.051 kb on - strand, within Pf6N2E2_1484 at 1734.259 kb on - strand, within Pf6N2E2_1484 at 1734.501 kb on + strand, within Pf6N2E2_1484 at 1734.761 kb on + strand at 1734.761 kb on + strand at 1734.761 kb on + strand at 1734.761 kb on + strand at 1734.762 kb on - strand at 1734.762 kb on - strand at 1734.762 kb on - strand at 1735.094 kb on + strand, within Pf6N2E2_1485 at 1735.094 kb on + strand, within Pf6N2E2_1485 at 1735.095 kb on - strand, within Pf6N2E2_1485 at 1735.095 kb on - strand, within Pf6N2E2_1485 at 1735.112 kb on + strand, within Pf6N2E2_1485 at 1735.112 kb on + strand, within Pf6N2E2_1485 at 1735.113 kb on - strand, within Pf6N2E2_1485 at 1735.265 kb on + strand, within Pf6N2E2_1485 at 1735.265 kb on + strand, within Pf6N2E2_1485 at 1735.265 kb on + strand, within Pf6N2E2_1485 at 1735.265 kb on + strand, within Pf6N2E2_1485 at 1735.265 kb on + strand at 1735.265 kb on + strand, within Pf6N2E2_1485 at 1735.564 kb on + strand, within Pf6N2E2_1485 at 1735.565 kb on - strand, within Pf6N2E2_1485 at 1735.600 kb on + strand, within Pf6N2E2_1485 at 1735.863 kb on - strand at 1735.863 kb on - strand at 1735.882 kb on - strand at 1735.882 kb on - strand at 1736.035 kb on + strand at 1736.036 kb on - strand at 1736.078 kb on - strand at 1736.226 kb on + strand, within Pf6N2E2_1486 at 1736.227 kb on - strand, within Pf6N2E2_1486 at 1736.246 kb on + strand, within Pf6N2E2_1486 at 1736.246 kb on + strand, within Pf6N2E2_1486 at 1736.387 kb on + strand, within Pf6N2E2_1486 at 1736.387 kb on + strand, within Pf6N2E2_1486 at 1736.387 kb on + strand, within Pf6N2E2_1486 at 1736.387 kb on + strand, within Pf6N2E2_1486 at 1736.387 kb on + strand, within Pf6N2E2_1486 at 1736.388 kb on - strand, within Pf6N2E2_1486 at 1736.388 kb on - strand, within Pf6N2E2_1486 at 1736.428 kb on + strand, within Pf6N2E2_1486 at 1736.524 kb on + strand, within Pf6N2E2_1486 at 1736.524 kb on + strand, within Pf6N2E2_1486 at 1736.525 kb on - strand, within Pf6N2E2_1486 at 1736.525 kb on - strand, within Pf6N2E2_1486 at 1736.606 kb on - strand, within Pf6N2E2_1486 at 1736.606 kb on - strand, within Pf6N2E2_1486
Per-strain Table
Position Strand Gene LocusTag Fraction Zinc chloride 0.0000015625 M remove 1,733,957 - Pf6N2E2_1484 0.12 -0.9 1,734,050 + Pf6N2E2_1484 0.22 -1.3 1,734,050 + Pf6N2E2_1484 0.22 -1.7 1,734,050 + Pf6N2E2_1484 0.22 +0.0 1,734,050 + Pf6N2E2_1484 0.22 +1.1 1,734,051 - Pf6N2E2_1484 0.22 +1.3 1,734,051 - Pf6N2E2_1484 0.22 +0.5 1,734,051 - Pf6N2E2_1484 0.22 +0.9 1,734,259 - Pf6N2E2_1484 0.44 +1.6 1,734,501 + Pf6N2E2_1484 0.70 -0.4 1,734,761 + +2.5 1,734,761 + +0.3 1,734,761 + +1.7 1,734,761 + +2.0 1,734,762 - +1.8 1,734,762 - -1.2 1,734,762 - -1.0 1,735,094 + Pf6N2E2_1485 0.23 -1.4 1,735,094 + Pf6N2E2_1485 0.23 +1.2 1,735,095 - Pf6N2E2_1485 0.23 -1.1 1,735,095 - Pf6N2E2_1485 0.23 -0.3 1,735,112 + Pf6N2E2_1485 0.25 -2.3 1,735,112 + Pf6N2E2_1485 0.25 -2.9 1,735,113 - Pf6N2E2_1485 0.25 -0.7 1,735,265 + Pf6N2E2_1485 0.42 -1.0 1,735,265 + Pf6N2E2_1485 0.42 +0.8 1,735,265 + Pf6N2E2_1485 0.42 +0.0 1,735,265 + Pf6N2E2_1485 0.42 -2.2 1,735,265 + +0.1 1,735,265 + Pf6N2E2_1485 0.42 -1.1 1,735,564 + Pf6N2E2_1485 0.76 +0.6 1,735,565 - Pf6N2E2_1485 0.76 +1.6 1,735,600 + Pf6N2E2_1485 0.80 +0.3 1,735,863 - -1.9 1,735,863 - -1.2 1,735,882 - -3.5 1,735,882 - +0.3 1,736,035 + +2.1 1,736,036 - +1.1 1,736,078 - +0.4 1,736,226 + Pf6N2E2_1486 0.16 +0.6 1,736,227 - Pf6N2E2_1486 0.16 -1.0 1,736,246 + Pf6N2E2_1486 0.17 +0.9 1,736,246 + Pf6N2E2_1486 0.17 -1.2 1,736,387 + Pf6N2E2_1486 0.25 -0.5 1,736,387 + Pf6N2E2_1486 0.25 -1.4 1,736,387 + Pf6N2E2_1486 0.25 -2.1 1,736,387 + Pf6N2E2_1486 0.25 +0.6 1,736,387 + Pf6N2E2_1486 0.25 +0.1 1,736,388 - Pf6N2E2_1486 0.25 +2.8 1,736,388 - Pf6N2E2_1486 0.25 +1.1 1,736,428 + Pf6N2E2_1486 0.27 -2.0 1,736,524 + Pf6N2E2_1486 0.32 +1.3 1,736,524 + Pf6N2E2_1486 0.32 +2.3 1,736,525 - Pf6N2E2_1486 0.32 -0.2 1,736,525 - Pf6N2E2_1486 0.32 -0.8 1,736,606 - Pf6N2E2_1486 0.37 +2.9 1,736,606 - Pf6N2E2_1486 0.37 +0.9
Or see this region's nucleotide sequence