Experiment: Survival; Treatment=FW300 (pH7.4) with 5.0 mM Glucose; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_18980 and IAI47_18985 overlap by 4 nucleotides IAI47_18985 and IAI47_18990 are separated by 137 nucleotides IAI47_18990 and IAI47_18995 are separated by 67 nucleotides IAI47_18995 and IAI47_19000 are separated by 33 nucleotides
IAI47_18980: IAI47_18980 - helix-turn-helix domain-containing protein, at 28,957 to 29,559
_18980
IAI47_18985: IAI47_18985 - MarR family transcriptional regulator, at 29,556 to 30,071
_18985
IAI47_18990: IAI47_18990 - MFS transporter, at 30,209 to 31,363
_18990
IAI47_18995: IAI47_18995 - aspartate/glutamate racemase family protein, at 31,431 to 32,123
_18995
IAI47_19000: IAI47_19000 - DHCW motif cupin fold protein, at 32,157 to 32,486
_19000
Position (kb) 30
31
32 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 29.313 kb on + strand, within IAI47_18980 at 29.325 kb on - strand, within IAI47_18980 at 29.325 kb on - strand, within IAI47_18980 at 29.484 kb on + strand, within IAI47_18980 at 29.484 kb on + strand, within IAI47_18980 at 29.484 kb on + strand, within IAI47_18980 at 29.484 kb on + strand, within IAI47_18980 at 29.485 kb on - strand, within IAI47_18980 at 29.485 kb on - strand, within IAI47_18980 at 29.497 kb on + strand, within IAI47_18980 at 29.497 kb on + strand, within IAI47_18980 at 29.497 kb on + strand, within IAI47_18980 at 29.498 kb on - strand, within IAI47_18980 at 29.498 kb on - strand, within IAI47_18980 at 29.498 kb on - strand, within IAI47_18980 at 29.498 kb on - strand, within IAI47_18980 at 29.523 kb on + strand at 29.579 kb on + strand at 29.580 kb on - strand at 29.580 kb on - strand at 29.580 kb on - strand at 29.580 kb on - strand at 29.750 kb on + strand, within IAI47_18985 at 29.750 kb on + strand, within IAI47_18985 at 29.751 kb on - strand, within IAI47_18985 at 29.751 kb on - strand, within IAI47_18985 at 29.751 kb on - strand, within IAI47_18985 at 29.751 kb on - strand, within IAI47_18985 at 29.751 kb on - strand, within IAI47_18985 at 29.830 kb on - strand, within IAI47_18985 at 29.845 kb on + strand, within IAI47_18985 at 29.901 kb on + strand, within IAI47_18985 at 29.901 kb on + strand, within IAI47_18985 at 29.902 kb on - strand, within IAI47_18985 at 29.902 kb on - strand, within IAI47_18985 at 29.902 kb on - strand, within IAI47_18985 at 29.902 kb on - strand, within IAI47_18985 at 29.902 kb on - strand, within IAI47_18985 at 29.902 kb on - strand, within IAI47_18985 at 30.059 kb on + strand at 30.071 kb on + strand at 30.071 kb on + strand at 30.071 kb on + strand at 30.071 kb on + strand at 30.071 kb on + strand at 30.071 kb on + strand at 30.072 kb on - strand at 30.072 kb on - strand at 30.082 kb on + strand at 30.082 kb on + strand at 30.095 kb on + strand at 30.095 kb on + strand at 30.095 kb on + strand at 30.095 kb on + strand at 30.095 kb on + strand at 30.096 kb on - strand at 30.096 kb on - strand at 30.122 kb on + strand at 30.122 kb on + strand at 30.122 kb on + strand at 30.123 kb on - strand at 30.123 kb on - strand at 30.123 kb on - strand at 30.123 kb on - strand at 30.264 kb on + strand at 30.286 kb on + strand at 30.287 kb on - strand at 30.287 kb on - strand at 30.338 kb on - strand, within IAI47_18990 at 30.418 kb on + strand, within IAI47_18990 at 30.586 kb on + strand, within IAI47_18990 at 30.586 kb on + strand, within IAI47_18990 at 30.586 kb on + strand, within IAI47_18990 at 30.587 kb on - strand, within IAI47_18990 at 30.632 kb on - strand, within IAI47_18990 at 30.632 kb on - strand, within IAI47_18990 at 30.720 kb on + strand, within IAI47_18990 at 30.721 kb on - strand, within IAI47_18990 at 30.721 kb on - strand, within IAI47_18990 at 30.721 kb on - strand, within IAI47_18990 at 30.738 kb on + strand, within IAI47_18990 at 30.739 kb on - strand, within IAI47_18990 at 30.739 kb on - strand, within IAI47_18990 at 30.822 kb on + strand, within IAI47_18990 at 30.822 kb on + strand, within IAI47_18990 at 30.822 kb on + strand, within IAI47_18990 at 30.857 kb on + strand, within IAI47_18990 at 30.890 kb on + strand, within IAI47_18990 at 30.890 kb on + strand, within IAI47_18990 at 30.890 kb on + strand, within IAI47_18990 at 30.890 kb on + strand, within IAI47_18990 at 30.890 kb on + strand, within IAI47_18990 at 30.890 kb on + strand, within IAI47_18990 at 30.891 kb on - strand, within IAI47_18990 at 30.891 kb on - strand, within IAI47_18990 at 30.891 kb on - strand, within IAI47_18990 at 30.891 kb on - strand, within IAI47_18990 at 30.891 kb on - strand, within IAI47_18990 at 30.891 kb on - strand, within IAI47_18990 at 30.891 kb on - strand, within IAI47_18990 at 30.891 kb on - strand, within IAI47_18990 at 30.892 kb on + strand, within IAI47_18990 at 30.893 kb on - strand, within IAI47_18990 at 30.894 kb on + strand, within IAI47_18990 at 30.894 kb on + strand, within IAI47_18990 at 30.894 kb on + strand, within IAI47_18990 at 30.894 kb on + strand, within IAI47_18990 at 30.894 kb on + strand, within IAI47_18990 at 30.894 kb on + strand, within IAI47_18990 at 30.894 kb on + strand, within IAI47_18990 at 30.895 kb on - strand, within IAI47_18990 at 30.895 kb on - strand, within IAI47_18990 at 30.895 kb on - strand, within IAI47_18990 at 30.895 kb on - strand, within IAI47_18990 at 30.895 kb on - strand, within IAI47_18990 at 30.970 kb on + strand, within IAI47_18990 at 30.971 kb on - strand, within IAI47_18990 at 30.971 kb on - strand, within IAI47_18990 at 30.987 kb on + strand, within IAI47_18990 at 30.987 kb on + strand, within IAI47_18990 at 30.987 kb on + strand, within IAI47_18990 at 30.988 kb on - strand, within IAI47_18990 at 30.988 kb on - strand, within IAI47_18990 at 30.988 kb on - strand, within IAI47_18990 at 30.988 kb on - strand, within IAI47_18990 at 31.039 kb on - strand, within IAI47_18990 at 31.068 kb on - strand, within IAI47_18990 at 31.108 kb on + strand, within IAI47_18990 at 31.109 kb on - strand, within IAI47_18990 at 31.317 kb on + strand at 31.317 kb on + strand at 31.318 kb on - strand at 31.318 kb on - strand at 31.318 kb on - strand at 31.366 kb on - strand at 31.407 kb on - strand at 31.407 kb on - strand at 31.407 kb on - strand at 31.407 kb on - strand at 31.440 kb on - strand at 31.440 kb on - strand at 31.457 kb on + strand at 31.457 kb on + strand at 31.457 kb on + strand at 31.482 kb on + strand at 31.482 kb on + strand at 31.483 kb on - strand at 31.483 kb on - strand at 31.483 kb on - strand at 31.492 kb on + strand at 31.551 kb on + strand, within IAI47_18995 at 31.551 kb on + strand, within IAI47_18995 at 31.551 kb on + strand, within IAI47_18995 at 31.551 kb on + strand, within IAI47_18995 at 31.551 kb on + strand, within IAI47_18995 at 31.551 kb on + strand, within IAI47_18995 at 31.551 kb on + strand, within IAI47_18995 at 31.551 kb on + strand, within IAI47_18995 at 31.551 kb on + strand, within IAI47_18995 at 31.552 kb on - strand, within IAI47_18995 at 31.552 kb on - strand, within IAI47_18995 at 31.552 kb on - strand, within IAI47_18995 at 31.552 kb on - strand, within IAI47_18995 at 31.552 kb on - strand, within IAI47_18995 at 31.552 kb on - strand, within IAI47_18995 at 31.553 kb on + strand, within IAI47_18995 at 31.554 kb on - strand, within IAI47_18995 at 31.554 kb on - strand, within IAI47_18995 at 31.554 kb on - strand, within IAI47_18995 at 31.554 kb on - strand, within IAI47_18995 at 31.554 kb on - strand, within IAI47_18995 at 31.571 kb on + strand, within IAI47_18995 at 31.571 kb on + strand, within IAI47_18995 at 31.619 kb on - strand, within IAI47_18995 at 31.619 kb on - strand, within IAI47_18995 at 31.637 kb on + strand, within IAI47_18995 at 31.638 kb on - strand, within IAI47_18995 at 31.670 kb on - strand, within IAI47_18995 at 31.670 kb on - strand, within IAI47_18995 at 31.670 kb on - strand, within IAI47_18995 at 31.676 kb on + strand, within IAI47_18995 at 31.691 kb on + strand, within IAI47_18995 at 31.691 kb on + strand, within IAI47_18995 at 31.691 kb on + strand, within IAI47_18995 at 31.691 kb on + strand, within IAI47_18995 at 31.691 kb on + strand, within IAI47_18995 at 31.692 kb on - strand, within IAI47_18995 at 31.692 kb on - strand, within IAI47_18995 at 31.739 kb on + strand, within IAI47_18995 at 31.739 kb on + strand, within IAI47_18995 at 31.740 kb on - strand, within IAI47_18995 at 31.740 kb on - strand, within IAI47_18995 at 31.764 kb on - strand, within IAI47_18995 at 31.764 kb on - strand, within IAI47_18995 at 31.784 kb on + strand, within IAI47_18995 at 31.784 kb on + strand, within IAI47_18995 at 31.784 kb on + strand, within IAI47_18995 at 31.785 kb on - strand, within IAI47_18995 at 31.785 kb on - strand, within IAI47_18995 at 31.785 kb on - strand, within IAI47_18995 at 31.785 kb on - strand, within IAI47_18995 at 31.914 kb on + strand, within IAI47_18995 at 31.915 kb on - strand, within IAI47_18995 at 31.915 kb on - strand, within IAI47_18995 at 32.030 kb on + strand, within IAI47_18995 at 32.031 kb on - strand, within IAI47_18995 at 32.075 kb on + strand at 32.075 kb on + strand at 32.075 kb on + strand at 32.076 kb on - strand at 32.076 kb on - strand at 32.076 kb on - strand at 32.076 kb on - strand at 32.076 kb on - strand at 32.076 kb on - strand at 32.078 kb on + strand at 32.078 kb on + strand at 32.078 kb on + strand at 32.079 kb on - strand at 32.079 kb on - strand at 32.079 kb on - strand at 32.079 kb on - strand at 32.121 kb on + strand at 32.121 kb on + strand at 32.164 kb on + strand at 32.165 kb on - strand at 32.169 kb on - strand at 32.289 kb on + strand, within IAI47_19000 at 32.301 kb on + strand, within IAI47_19000 at 32.301 kb on + strand, within IAI47_19000 at 32.301 kb on + strand, within IAI47_19000 at 32.301 kb on + strand, within IAI47_19000 at 32.301 kb on + strand, within IAI47_19000 at 32.333 kb on + strand, within IAI47_19000 at 32.333 kb on + strand, within IAI47_19000 at 32.333 kb on + strand, within IAI47_19000 at 32.333 kb on + strand, within IAI47_19000 at 32.334 kb on - strand, within IAI47_19000 at 32.334 kb on - strand, within IAI47_19000 at 32.345 kb on + strand, within IAI47_19000 at 32.346 kb on - strand, within IAI47_19000
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 5.0 mM Glucose; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 29,313 + IAI47_18980 0.59 -1.1 29,325 - IAI47_18980 0.61 +1.1 29,325 - IAI47_18980 0.61 -0.2 29,484 + IAI47_18980 0.87 -1.7 29,484 + IAI47_18980 0.87 +1.6 29,484 + IAI47_18980 0.87 -0.2 29,484 + IAI47_18980 0.87 -0.9 29,485 - IAI47_18980 0.88 +0.2 29,485 - IAI47_18980 0.88 +0.7 29,497 + IAI47_18980 0.90 +0.6 29,497 + IAI47_18980 0.90 +0.6 29,497 + IAI47_18980 0.90 +0.8 29,498 - IAI47_18980 0.90 -1.1 29,498 - IAI47_18980 0.90 -0.4 29,498 - IAI47_18980 0.90 +0.3 29,498 - IAI47_18980 0.90 +0.4 29,523 + +0.9 29,579 + +0.1 29,580 - -0.9 29,580 - -1.2 29,580 - +0.2 29,580 - +0.6 29,750 + IAI47_18985 0.38 -1.7 29,750 + IAI47_18985 0.38 +1.6 29,751 - IAI47_18985 0.38 +1.8 29,751 - IAI47_18985 0.38 +0.5 29,751 - IAI47_18985 0.38 +1.9 29,751 - IAI47_18985 0.38 +0.5 29,751 - IAI47_18985 0.38 -0.1 29,830 - IAI47_18985 0.53 +1.2 29,845 + IAI47_18985 0.56 -0.7 29,901 + IAI47_18985 0.67 -0.5 29,901 + IAI47_18985 0.67 +1.2 29,902 - IAI47_18985 0.67 +1.6 29,902 - IAI47_18985 0.67 -0.1 29,902 - IAI47_18985 0.67 +0.6 29,902 - IAI47_18985 0.67 -2.0 29,902 - IAI47_18985 0.67 +0.2 29,902 - IAI47_18985 0.67 -0.6 30,059 + -0.8 30,071 + -0.2 30,071 + +0.5 30,071 + +1.2 30,071 + +0.8 30,071 + +0.7 30,071 + -1.5 30,072 - -0.5 30,072 - -0.5 30,082 + +3.5 30,082 + -1.1 30,095 + +0.1 30,095 + +0.8 30,095 + -0.6 30,095 + +0.1 30,095 + +2.4 30,096 - -0.4 30,096 - +0.5 30,122 + +0.8 30,122 + -0.0 30,122 + +0.2 30,123 - +0.9 30,123 - +1.2 30,123 - +0.9 30,123 - +1.8 30,264 + +0.5 30,286 + +0.7 30,287 - +1.0 30,287 - -0.7 30,338 - IAI47_18990 0.11 +1.1 30,418 + IAI47_18990 0.18 +0.6 30,586 + IAI47_18990 0.33 -0.8 30,586 + IAI47_18990 0.33 -0.1 30,586 + IAI47_18990 0.33 +1.3 30,587 - IAI47_18990 0.33 +0.2 30,632 - IAI47_18990 0.37 +1.2 30,632 - IAI47_18990 0.37 +0.5 30,720 + IAI47_18990 0.44 +1.6 30,721 - IAI47_18990 0.44 +0.5 30,721 - IAI47_18990 0.44 +0.8 30,721 - IAI47_18990 0.44 +0.1 30,738 + IAI47_18990 0.46 +1.1 30,739 - IAI47_18990 0.46 -0.0 30,739 - IAI47_18990 0.46 -0.9 30,822 + IAI47_18990 0.53 -0.5 30,822 + IAI47_18990 0.53 -0.9 30,822 + IAI47_18990 0.53 +1.6 30,857 + IAI47_18990 0.56 +1.8 30,890 + IAI47_18990 0.59 -1.0 30,890 + IAI47_18990 0.59 +2.0 30,890 + IAI47_18990 0.59 +1.0 30,890 + IAI47_18990 0.59 +2.0 30,890 + IAI47_18990 0.59 +1.5 30,890 + IAI47_18990 0.59 -0.3 30,891 - IAI47_18990 0.59 +0.9 30,891 - IAI47_18990 0.59 -1.3 30,891 - IAI47_18990 0.59 -0.2 30,891 - IAI47_18990 0.59 +2.8 30,891 - IAI47_18990 0.59 +0.5 30,891 - IAI47_18990 0.59 +0.9 30,891 - IAI47_18990 0.59 +0.6 30,891 - IAI47_18990 0.59 +0.3 30,892 + IAI47_18990 0.59 +1.6 30,893 - IAI47_18990 0.59 +1.2 30,894 + IAI47_18990 0.59 +0.1 30,894 + IAI47_18990 0.59 +0.6 30,894 + IAI47_18990 0.59 +0.4 30,894 + IAI47_18990 0.59 +0.3 30,894 + IAI47_18990 0.59 -1.1 30,894 + IAI47_18990 0.59 +1.2 30,894 + IAI47_18990 0.59 +2.5 30,895 - IAI47_18990 0.59 +0.8 30,895 - IAI47_18990 0.59 +1.3 30,895 - IAI47_18990 0.59 +0.7 30,895 - IAI47_18990 0.59 +0.2 30,895 - IAI47_18990 0.59 +0.4 30,970 + IAI47_18990 0.66 +0.7 30,971 - IAI47_18990 0.66 -0.5 30,971 - IAI47_18990 0.66 +0.5 30,987 + IAI47_18990 0.67 +0.5 30,987 + IAI47_18990 0.67 -1.3 30,987 + IAI47_18990 0.67 +1.0 30,988 - IAI47_18990 0.67 -1.1 30,988 - IAI47_18990 0.67 -0.2 30,988 - IAI47_18990 0.67 -0.0 30,988 - IAI47_18990 0.67 +0.2 31,039 - IAI47_18990 0.72 +0.5 31,068 - IAI47_18990 0.74 +1.3 31,108 + IAI47_18990 0.78 +1.2 31,109 - IAI47_18990 0.78 +2.8 31,317 + +1.1 31,317 + -0.4 31,318 - -0.0 31,318 - -0.4 31,318 - -0.5 31,366 - +1.5 31,407 - +0.2 31,407 - -0.7 31,407 - -0.7 31,407 - +0.5 31,440 - +0.2 31,440 - -0.7 31,457 + +0.5 31,457 + +0.4 31,457 + +3.8 31,482 + +0.7 31,482 + -1.5 31,483 - -0.7 31,483 - +0.7 31,483 - -0.4 31,492 + -0.0 31,551 + IAI47_18995 0.17 -2.0 31,551 + IAI47_18995 0.17 +0.2 31,551 + IAI47_18995 0.17 +0.3 31,551 + IAI47_18995 0.17 -2.0 31,551 + IAI47_18995 0.17 -1.1 31,551 + IAI47_18995 0.17 +0.1 31,551 + IAI47_18995 0.17 +2.2 31,551 + IAI47_18995 0.17 -1.3 31,551 + IAI47_18995 0.17 -0.0 31,552 - IAI47_18995 0.17 +0.8 31,552 - IAI47_18995 0.17 -1.0 31,552 - IAI47_18995 0.17 +2.0 31,552 - IAI47_18995 0.17 -0.1 31,552 - IAI47_18995 0.17 -0.4 31,552 - IAI47_18995 0.17 -0.5 31,553 + IAI47_18995 0.18 +0.8 31,554 - IAI47_18995 0.18 +1.0 31,554 - IAI47_18995 0.18 +0.1 31,554 - IAI47_18995 0.18 -1.0 31,554 - IAI47_18995 0.18 +0.3 31,554 - IAI47_18995 0.18 -0.5 31,571 + IAI47_18995 0.20 -0.5 31,571 + IAI47_18995 0.20 +0.6 31,619 - IAI47_18995 0.27 -0.4 31,619 - IAI47_18995 0.27 -1.0 31,637 + IAI47_18995 0.30 +0.2 31,638 - IAI47_18995 0.30 +0.2 31,670 - IAI47_18995 0.34 -1.2 31,670 - IAI47_18995 0.34 +1.4 31,670 - IAI47_18995 0.34 -0.7 31,676 + IAI47_18995 0.35 -0.5 31,691 + IAI47_18995 0.38 -0.5 31,691 + IAI47_18995 0.38 +1.6 31,691 + IAI47_18995 0.38 +0.9 31,691 + IAI47_18995 0.38 -2.4 31,691 + IAI47_18995 0.38 -0.0 31,692 - IAI47_18995 0.38 -0.7 31,692 - IAI47_18995 0.38 -2.0 31,739 + IAI47_18995 0.44 +0.7 31,739 + IAI47_18995 0.44 +0.4 31,740 - IAI47_18995 0.45 -0.7 31,740 - IAI47_18995 0.45 -1.4 31,764 - IAI47_18995 0.48 +0.1 31,764 - IAI47_18995 0.48 -0.0 31,784 + IAI47_18995 0.51 -0.1 31,784 + IAI47_18995 0.51 -0.2 31,784 + IAI47_18995 0.51 +2.5 31,785 - IAI47_18995 0.51 +1.4 31,785 - IAI47_18995 0.51 -0.6 31,785 - IAI47_18995 0.51 -1.4 31,785 - IAI47_18995 0.51 -0.8 31,914 + IAI47_18995 0.70 +0.8 31,915 - IAI47_18995 0.70 -1.5 31,915 - IAI47_18995 0.70 -1.7 32,030 + IAI47_18995 0.86 -0.3 32,031 - IAI47_18995 0.87 +1.1 32,075 + +0.7 32,075 + -0.0 32,075 + -0.3 32,076 - -0.3 32,076 - -0.1 32,076 - -1.7 32,076 - +1.1 32,076 - -0.4 32,076 - +0.9 32,078 + +2.8 32,078 + -1.8 32,078 + +0.4 32,079 - +1.1 32,079 - +0.3 32,079 - -0.7 32,079 - -0.3 32,121 + +0.5 32,121 + -1.0 32,164 + +0.3 32,165 - +2.1 32,169 - -1.4 32,289 + IAI47_19000 0.40 +0.8 32,301 + IAI47_19000 0.44 +1.0 32,301 + IAI47_19000 0.44 +0.5 32,301 + IAI47_19000 0.44 +0.8 32,301 + IAI47_19000 0.44 +1.5 32,301 + IAI47_19000 0.44 -1.9 32,333 + IAI47_19000 0.53 +1.0 32,333 + IAI47_19000 0.53 -0.6 32,333 + IAI47_19000 0.53 +1.3 32,333 + IAI47_19000 0.53 -0.7 32,334 - IAI47_19000 0.54 -0.1 32,334 - IAI47_19000 0.54 +0.2 32,345 + IAI47_19000 0.57 +1.2 32,346 - IAI47_19000 0.57 +1.0
Or see this region's nucleotide sequence