Experiment: Survival; Treatment=FW300 (pH7.4) with 5.0 mM Glucose; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_04055 and IAI47_04060 are separated by 587 nucleotides IAI47_04060 and IAI47_04065 overlap by 1 nucleotides IAI47_04065 and IAI47_04070 are separated by 12 nucleotides IAI47_04070 and IAI47_04075 are separated by 67 nucleotides
IAI47_04055: IAI47_04055 - SecY-interacting protein, at 847,690 to 848,238
_04055
IAI47_04060: IAI47_04060 - YqcC family protein, at 848,826 to 849,155
_04060
IAI47_04065: IAI47_04065 - tRNA pseudouridine(65) synthase TruC, at 849,155 to 849,934
_04065
IAI47_04070: IAI47_04070 - flavodoxin, at 849,947 to 850,393
_04070
IAI47_04075: IAI47_04075 - galactarate dehydratase, at 850,461 to 852,020
_04075
Position (kb) 849
850 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 848.219 kb on + strand at 848.219 kb on + strand at 848.258 kb on + strand at 848.258 kb on + strand at 848.259 kb on - strand at 848.259 kb on - strand at 848.270 kb on + strand at 848.270 kb on + strand at 848.270 kb on + strand at 848.270 kb on + strand at 848.271 kb on - strand at 848.277 kb on + strand at 848.277 kb on + strand at 848.277 kb on + strand at 848.277 kb on + strand at 848.278 kb on - strand at 848.278 kb on - strand at 848.301 kb on + strand at 848.301 kb on + strand at 848.301 kb on + strand at 848.301 kb on + strand at 848.302 kb on - strand at 848.302 kb on - strand at 848.303 kb on + strand at 848.303 kb on + strand at 848.303 kb on + strand at 848.303 kb on + strand at 848.303 kb on + strand at 848.303 kb on + strand at 848.303 kb on + strand at 848.303 kb on + strand at 848.304 kb on - strand at 848.304 kb on - strand at 848.304 kb on - strand at 848.304 kb on - strand at 848.310 kb on + strand at 848.310 kb on + strand at 848.310 kb on + strand at 848.311 kb on - strand at 848.311 kb on - strand at 848.315 kb on + strand at 848.316 kb on - strand at 848.316 kb on - strand at 848.358 kb on + strand at 848.358 kb on + strand at 848.359 kb on - strand at 848.359 kb on - strand at 848.368 kb on + strand at 848.376 kb on + strand at 848.411 kb on + strand at 848.411 kb on + strand at 848.412 kb on - strand at 848.412 kb on - strand at 848.412 kb on - strand at 848.474 kb on + strand at 848.474 kb on + strand at 848.474 kb on + strand at 848.475 kb on - strand at 848.491 kb on + strand at 848.491 kb on + strand at 848.491 kb on + strand at 848.491 kb on + strand at 848.492 kb on - strand at 848.492 kb on - strand at 848.492 kb on - strand at 848.492 kb on - strand at 848.492 kb on - strand at 848.511 kb on + strand at 848.512 kb on - strand at 848.521 kb on + strand at 848.570 kb on + strand at 848.571 kb on - strand at 848.631 kb on + strand at 848.637 kb on + strand at 848.637 kb on + strand at 848.637 kb on + strand at 848.637 kb on + strand at 848.637 kb on + strand at 848.637 kb on + strand at 848.637 kb on + strand at 848.637 kb on + strand at 848.637 kb on + strand at 848.637 kb on + strand at 848.638 kb on - strand at 848.638 kb on - strand at 848.638 kb on - strand at 848.701 kb on + strand at 848.701 kb on + strand at 848.701 kb on + strand at 848.701 kb on + strand at 848.701 kb on + strand at 848.702 kb on - strand at 848.702 kb on - strand at 848.707 kb on + strand at 848.708 kb on - strand at 848.715 kb on + strand at 848.716 kb on - strand at 848.743 kb on + strand at 848.743 kb on + strand at 848.744 kb on - strand at 848.745 kb on + strand at 848.745 kb on + strand at 848.745 kb on + strand at 848.745 kb on + strand at 848.745 kb on + strand at 848.745 kb on + strand at 848.745 kb on + strand at 848.746 kb on - strand at 848.746 kb on - strand at 848.746 kb on - strand at 848.746 kb on - strand at 848.783 kb on + strand at 848.784 kb on + strand at 848.784 kb on + strand at 848.784 kb on + strand at 848.784 kb on + strand at 848.784 kb on + strand at 848.784 kb on + strand at 848.785 kb on - strand at 848.785 kb on - strand at 848.842 kb on + strand at 848.843 kb on - strand at 848.843 kb on - strand at 848.843 kb on - strand at 848.843 kb on - strand at 848.846 kb on + strand at 848.846 kb on + strand at 848.846 kb on + strand at 848.846 kb on + strand at 848.903 kb on + strand, within IAI47_04060 at 848.903 kb on + strand, within IAI47_04060 at 848.903 kb on + strand, within IAI47_04060 at 848.903 kb on + strand, within IAI47_04060 at 848.903 kb on + strand, within IAI47_04060 at 848.903 kb on + strand, within IAI47_04060 at 848.904 kb on - strand, within IAI47_04060 at 848.928 kb on - strand, within IAI47_04060 at 848.928 kb on - strand, within IAI47_04060 at 848.954 kb on + strand, within IAI47_04060 at 848.954 kb on + strand, within IAI47_04060 at 848.954 kb on + strand, within IAI47_04060 at 848.954 kb on - strand, within IAI47_04060 at 848.955 kb on - strand, within IAI47_04060 at 848.955 kb on - strand, within IAI47_04060 at 848.989 kb on + strand, within IAI47_04060 at 848.990 kb on - strand, within IAI47_04060 at 848.990 kb on - strand, within IAI47_04060 at 848.990 kb on - strand, within IAI47_04060 at 848.990 kb on - strand, within IAI47_04060 at 849.057 kb on + strand, within IAI47_04060 at 849.057 kb on + strand, within IAI47_04060 at 849.058 kb on - strand, within IAI47_04060 at 849.058 kb on - strand, within IAI47_04060 at 849.060 kb on + strand, within IAI47_04060 at 849.060 kb on + strand, within IAI47_04060 at 849.060 kb on + strand, within IAI47_04060 at 849.060 kb on + strand, within IAI47_04060 at 849.061 kb on - strand, within IAI47_04060 at 849.061 kb on - strand, within IAI47_04060 at 849.061 kb on - strand, within IAI47_04060 at 849.061 kb on - strand, within IAI47_04060 at 849.165 kb on + strand at 849.165 kb on + strand at 849.165 kb on + strand at 849.165 kb on + strand at 849.165 kb on + strand at 849.165 kb on + strand at 849.165 kb on + strand at 849.165 kb on + strand at 849.166 kb on - strand at 849.166 kb on - strand at 849.166 kb on - strand at 849.166 kb on - strand at 849.166 kb on - strand at 849.166 kb on - strand at 849.166 kb on - strand at 849.166 kb on - strand at 849.170 kb on + strand at 849.170 kb on + strand at 849.170 kb on + strand at 849.170 kb on + strand at 849.171 kb on - strand at 849.171 kb on - strand at 849.171 kb on - strand at 849.171 kb on - strand at 849.171 kb on - strand at 849.197 kb on - strand at 849.316 kb on + strand, within IAI47_04065 at 849.317 kb on - strand, within IAI47_04065 at 849.539 kb on + strand, within IAI47_04065 at 849.540 kb on - strand, within IAI47_04065 at 849.599 kb on + strand, within IAI47_04065 at 849.600 kb on - strand, within IAI47_04065 at 849.609 kb on + strand, within IAI47_04065 at 849.610 kb on - strand, within IAI47_04065 at 849.610 kb on - strand, within IAI47_04065 at 849.610 kb on - strand, within IAI47_04065 at 849.610 kb on - strand, within IAI47_04065 at 849.610 kb on - strand, within IAI47_04065 at 849.610 kb on - strand, within IAI47_04065 at 849.622 kb on + strand, within IAI47_04065 at 849.634 kb on + strand, within IAI47_04065 at 849.634 kb on + strand, within IAI47_04065 at 849.634 kb on + strand, within IAI47_04065 at 849.634 kb on + strand, within IAI47_04065 at 849.634 kb on + strand, within IAI47_04065 at 849.635 kb on - strand, within IAI47_04065 at 849.635 kb on - strand, within IAI47_04065 at 849.635 kb on - strand, within IAI47_04065 at 849.655 kb on + strand, within IAI47_04065 at 849.655 kb on + strand, within IAI47_04065 at 849.655 kb on + strand, within IAI47_04065 at 849.655 kb on + strand, within IAI47_04065 at 849.655 kb on + strand, within IAI47_04065 at 849.655 kb on + strand, within IAI47_04065 at 849.655 kb on + strand, within IAI47_04065 at 849.655 kb on + strand, within IAI47_04065 at 849.655 kb on + strand, within IAI47_04065 at 849.655 kb on + strand, within IAI47_04065 at 849.655 kb on + strand, within IAI47_04065 at 849.655 kb on + strand, within IAI47_04065 at 849.655 kb on + strand, within IAI47_04065 at 849.656 kb on - strand, within IAI47_04065 at 849.656 kb on - strand, within IAI47_04065 at 849.656 kb on - strand, within IAI47_04065 at 849.656 kb on - strand, within IAI47_04065 at 849.656 kb on - strand, within IAI47_04065 at 849.656 kb on - strand, within IAI47_04065 at 849.656 kb on - strand, within IAI47_04065 at 849.656 kb on - strand, within IAI47_04065 at 849.656 kb on - strand, within IAI47_04065 at 849.656 kb on - strand, within IAI47_04065 at 849.656 kb on - strand, within IAI47_04065 at 849.656 kb on - strand, within IAI47_04065 at 849.679 kb on + strand, within IAI47_04065 at 849.810 kb on + strand, within IAI47_04065 at 849.810 kb on + strand, within IAI47_04065 at 849.932 kb on + strand at 849.933 kb on - strand at 849.969 kb on + strand at 849.973 kb on + strand at 849.973 kb on + strand at 849.973 kb on + strand at 849.973 kb on - strand at 849.974 kb on - strand at 849.974 kb on - strand at 849.974 kb on - strand at 849.974 kb on - strand at 849.974 kb on - strand at 849.974 kb on - strand at 849.974 kb on - strand at 849.974 kb on - strand at 849.974 kb on - strand at 849.978 kb on + strand at 849.978 kb on + strand at 849.979 kb on - strand at 849.979 kb on - strand at 849.980 kb on + strand at 849.980 kb on + strand at 849.980 kb on + strand at 849.981 kb on - strand at 850.020 kb on + strand, within IAI47_04070 at 850.050 kb on + strand, within IAI47_04070 at 850.050 kb on + strand, within IAI47_04070 at 850.050 kb on + strand, within IAI47_04070 at 850.050 kb on + strand, within IAI47_04070 at 850.050 kb on + strand, within IAI47_04070 at 850.050 kb on + strand, within IAI47_04070 at 850.051 kb on - strand, within IAI47_04070 at 850.051 kb on - strand, within IAI47_04070 at 850.178 kb on - strand, within IAI47_04070 at 850.178 kb on - strand, within IAI47_04070 at 850.178 kb on - strand, within IAI47_04070 at 850.235 kb on + strand, within IAI47_04070 at 850.235 kb on + strand, within IAI47_04070 at 850.235 kb on + strand, within IAI47_04070 at 850.314 kb on + strand, within IAI47_04070 at 850.314 kb on + strand, within IAI47_04070 at 850.315 kb on - strand, within IAI47_04070 at 850.409 kb on + strand at 850.409 kb on + strand at 850.409 kb on + strand at 850.409 kb on + strand at 850.409 kb on + strand at 850.409 kb on + strand at 850.410 kb on - strand at 850.410 kb on - strand at 850.410 kb on - strand at 850.410 kb on - strand at 850.428 kb on + strand at 850.429 kb on - strand at 850.429 kb on - strand at 850.434 kb on + strand at 850.434 kb on + strand at 850.434 kb on + strand at 850.434 kb on + strand at 850.434 kb on + strand at 850.435 kb on - strand at 850.589 kb on + strand at 850.589 kb on + strand at 850.589 kb on + strand at 850.590 kb on - strand at 850.590 kb on - strand at 850.702 kb on + strand, within IAI47_04075 at 850.735 kb on - strand, within IAI47_04075 at 850.735 kb on - strand, within IAI47_04075 at 850.735 kb on - strand, within IAI47_04075 at 850.798 kb on + strand, within IAI47_04075 at 850.798 kb on + strand, within IAI47_04075 at 850.803 kb on + strand, within IAI47_04075 at 850.818 kb on - strand, within IAI47_04075 at 850.896 kb on + strand, within IAI47_04075 at 850.897 kb on - strand, within IAI47_04075 at 850.897 kb on - strand, within IAI47_04075
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 5.0 mM Glucose; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 848,219 + -1.1 848,219 + -0.6 848,258 + -2.4 848,258 + +0.1 848,259 - +0.1 848,259 - -0.3 848,270 + -1.0 848,270 + +0.4 848,270 + -1.3 848,270 + -1.6 848,271 - +0.5 848,277 + -0.8 848,277 + -0.4 848,277 + -0.3 848,277 + +0.4 848,278 - -2.5 848,278 - -3.1 848,301 + -0.6 848,301 + -0.7 848,301 + -1.4 848,301 + -2.4 848,302 - -0.2 848,302 - -1.4 848,303 + -0.5 848,303 + -0.9 848,303 + -1.3 848,303 + -1.0 848,303 + -0.6 848,303 + -0.6 848,303 + +0.3 848,303 + -1.7 848,304 - -3.1 848,304 - -1.8 848,304 - -0.8 848,304 - -1.1 848,310 + -0.8 848,310 + -1.6 848,310 + -0.4 848,311 - -0.6 848,311 - +0.2 848,315 + -0.9 848,316 - -2.3 848,316 - -1.7 848,358 + +1.0 848,358 + -1.1 848,359 - -1.3 848,359 - -0.2 848,368 + -0.0 848,376 + +0.2 848,411 + +0.7 848,411 + +1.3 848,412 - -0.4 848,412 - -0.5 848,412 - -1.3 848,474 + +1.1 848,474 + +0.9 848,474 + -0.5 848,475 - -2.7 848,491 + +1.1 848,491 + +0.0 848,491 + +0.4 848,491 + +0.5 848,492 - -3.8 848,492 - -2.0 848,492 - -1.7 848,492 - -2.9 848,492 - -3.9 848,511 + +0.3 848,512 - -2.3 848,521 + -0.3 848,570 + +2.4 848,571 - -3.3 848,631 + -0.3 848,637 + +0.5 848,637 + -0.6 848,637 + +0.5 848,637 + -0.6 848,637 + +0.2 848,637 + +1.0 848,637 + +0.2 848,637 + +0.6 848,637 + -2.9 848,637 + +1.5 848,638 - -3.6 848,638 - -1.0 848,638 - -1.9 848,701 + -1.3 848,701 + +1.0 848,701 + -0.8 848,701 + -0.3 848,701 + -0.4 848,702 - -4.3 848,702 - -2.3 848,707 + +0.9 848,708 - -2.1 848,715 + +0.3 848,716 - -1.7 848,743 + -0.1 848,743 + -1.0 848,744 - -2.8 848,745 + -0.1 848,745 + +1.0 848,745 + -1.3 848,745 + +0.5 848,745 + -1.1 848,745 + -0.7 848,745 + +0.0 848,746 - -2.8 848,746 - -2.4 848,746 - -1.2 848,746 - -3.1 848,783 + +0.1 848,784 + +0.5 848,784 + -0.2 848,784 + +0.1 848,784 + +1.5 848,784 + -1.0 848,784 + +0.5 848,785 - -1.1 848,785 - +2.4 848,842 + +0.9 848,843 - -0.9 848,843 - +0.6 848,843 - +3.3 848,843 - +0.4 848,846 + -0.8 848,846 + -0.0 848,846 + +0.9 848,846 + +0.3 848,903 + IAI47_04060 0.23 -0.6 848,903 + IAI47_04060 0.23 +0.2 848,903 + IAI47_04060 0.23 +0.9 848,903 + IAI47_04060 0.23 -0.0 848,903 + IAI47_04060 0.23 +0.6 848,903 + IAI47_04060 0.23 +1.5 848,904 - IAI47_04060 0.24 -0.3 848,928 - IAI47_04060 0.31 +0.5 848,928 - IAI47_04060 0.31 +1.7 848,954 + IAI47_04060 0.39 +0.1 848,954 + IAI47_04060 0.39 +2.2 848,954 + IAI47_04060 0.39 +1.2 848,954 - IAI47_04060 0.39 +1.2 848,955 - IAI47_04060 0.39 -1.7 848,955 - IAI47_04060 0.39 -0.1 848,989 + IAI47_04060 0.49 -1.5 848,990 - IAI47_04060 0.50 +0.6 848,990 - IAI47_04060 0.50 +0.2 848,990 - IAI47_04060 0.50 -0.7 848,990 - IAI47_04060 0.50 -1.0 849,057 + IAI47_04060 0.70 -0.0 849,057 + IAI47_04060 0.70 +0.8 849,058 - IAI47_04060 0.70 +1.2 849,058 - IAI47_04060 0.70 +0.5 849,060 + IAI47_04060 0.71 +2.5 849,060 + IAI47_04060 0.71 +1.7 849,060 + IAI47_04060 0.71 +0.5 849,060 + IAI47_04060 0.71 -0.9 849,061 - IAI47_04060 0.71 -0.6 849,061 - IAI47_04060 0.71 -0.3 849,061 - IAI47_04060 0.71 -1.9 849,061 - IAI47_04060 0.71 +0.9 849,165 + +0.4 849,165 + -0.8 849,165 + +0.3 849,165 + +1.0 849,165 + -2.0 849,165 + -0.9 849,165 + -0.1 849,165 + -2.3 849,166 - -0.9 849,166 - -0.4 849,166 - +0.4 849,166 - -1.5 849,166 - -0.5 849,166 - -0.6 849,166 - -0.0 849,166 - +0.7 849,170 + +0.8 849,170 + +0.2 849,170 + +1.3 849,170 + +0.7 849,171 - -0.7 849,171 - +0.7 849,171 - -0.0 849,171 - -0.0 849,171 - +0.7 849,197 - +0.4 849,316 + IAI47_04065 0.21 +2.2 849,317 - IAI47_04065 0.21 +1.2 849,539 + IAI47_04065 0.49 +0.2 849,540 - IAI47_04065 0.49 -0.3 849,599 + IAI47_04065 0.57 +1.0 849,600 - IAI47_04065 0.57 -0.2 849,609 + IAI47_04065 0.58 -0.2 849,610 - IAI47_04065 0.58 +0.1 849,610 - IAI47_04065 0.58 -0.3 849,610 - IAI47_04065 0.58 -1.0 849,610 - IAI47_04065 0.58 -0.1 849,610 - IAI47_04065 0.58 -0.6 849,610 - IAI47_04065 0.58 -0.4 849,622 + IAI47_04065 0.60 +1.3 849,634 + IAI47_04065 0.61 -0.3 849,634 + IAI47_04065 0.61 -1.0 849,634 + IAI47_04065 0.61 +0.2 849,634 + IAI47_04065 0.61 +0.1 849,634 + IAI47_04065 0.61 +0.8 849,635 - IAI47_04065 0.62 -0.2 849,635 - IAI47_04065 0.62 -0.4 849,635 - IAI47_04065 0.62 -0.2 849,655 + IAI47_04065 0.64 +2.5 849,655 + IAI47_04065 0.64 +0.5 849,655 + IAI47_04065 0.64 -1.9 849,655 + IAI47_04065 0.64 +0.7 849,655 + IAI47_04065 0.64 +1.1 849,655 + IAI47_04065 0.64 -0.5 849,655 + IAI47_04065 0.64 +4.0 849,655 + IAI47_04065 0.64 +1.7 849,655 + IAI47_04065 0.64 +3.4 849,655 + IAI47_04065 0.64 -0.0 849,655 + IAI47_04065 0.64 +0.1 849,655 + IAI47_04065 0.64 +1.4 849,655 + IAI47_04065 0.64 +3.6 849,656 - IAI47_04065 0.64 -0.3 849,656 - IAI47_04065 0.64 +0.1 849,656 - IAI47_04065 0.64 +0.2 849,656 - IAI47_04065 0.64 -1.0 849,656 - IAI47_04065 0.64 +0.6 849,656 - IAI47_04065 0.64 +0.0 849,656 - IAI47_04065 0.64 +0.9 849,656 - IAI47_04065 0.64 +0.3 849,656 - IAI47_04065 0.64 -0.1 849,656 - IAI47_04065 0.64 -0.6 849,656 - IAI47_04065 0.64 -0.2 849,656 - IAI47_04065 0.64 -0.2 849,679 + IAI47_04065 0.67 +4.2 849,810 + IAI47_04065 0.84 -0.6 849,810 + IAI47_04065 0.84 -0.1 849,932 + +0.8 849,933 - -0.5 849,969 + +0.4 849,973 + +0.4 849,973 + +0.3 849,973 + +0.3 849,973 - -0.1 849,974 - +0.3 849,974 - -1.2 849,974 - -1.8 849,974 - -2.2 849,974 - -0.6 849,974 - -1.7 849,974 - -2.1 849,974 - -1.5 849,974 - -1.4 849,978 + -0.1 849,978 + +0.7 849,979 - -1.1 849,979 - +0.1 849,980 + +0.0 849,980 + -0.6 849,980 + +0.9 849,981 - -0.5 850,020 + IAI47_04070 0.16 +2.7 850,050 + IAI47_04070 0.23 -0.2 850,050 + IAI47_04070 0.23 +1.3 850,050 + IAI47_04070 0.23 +0.9 850,050 + IAI47_04070 0.23 -1.8 850,050 + IAI47_04070 0.23 -0.1 850,050 + IAI47_04070 0.23 -1.0 850,051 - IAI47_04070 0.23 -1.1 850,051 - IAI47_04070 0.23 -0.1 850,178 - IAI47_04070 0.52 -0.9 850,178 - IAI47_04070 0.52 +0.3 850,178 - IAI47_04070 0.52 +0.3 850,235 + IAI47_04070 0.64 -0.0 850,235 + IAI47_04070 0.64 +2.9 850,235 + IAI47_04070 0.64 +1.3 850,314 + IAI47_04070 0.82 +0.5 850,314 + IAI47_04070 0.82 +1.5 850,315 - IAI47_04070 0.82 -1.7 850,409 + -0.3 850,409 + -0.5 850,409 + +0.2 850,409 + -0.2 850,409 + +0.6 850,409 + -0.5 850,410 - -1.2 850,410 - -2.0 850,410 - -0.3 850,410 - +1.2 850,428 + -1.2 850,429 - +1.9 850,429 - -1.7 850,434 + -0.5 850,434 + +0.2 850,434 + -0.7 850,434 + +0.9 850,434 + +0.2 850,435 - -2.1 850,589 + -0.1 850,589 + -0.6 850,589 + -1.6 850,590 - +0.2 850,590 - -0.7 850,702 + IAI47_04075 0.15 +0.2 850,735 - IAI47_04075 0.18 +1.1 850,735 - IAI47_04075 0.18 +0.3 850,735 - IAI47_04075 0.18 -0.1 850,798 + IAI47_04075 0.22 -1.3 850,798 + IAI47_04075 0.22 +0.5 850,803 + IAI47_04075 0.22 -1.0 850,818 - IAI47_04075 0.23 -1.4 850,896 + IAI47_04075 0.28 -0.0 850,897 - IAI47_04075 0.28 +0.6 850,897 - IAI47_04075 0.28 -0.2
Or see this region's nucleotide sequence