Experiment: BG11 with Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_2179 and prxQ-A1 are separated by 130 nucleotides prxQ-A1 and Synpcc7942_2181 are separated by 52 nucleotides Synpcc7942_2181 and Synpcc7942_2182 are separated by 52 nucleotides Synpcc7942_2182 and rnhA are separated by 126 nucleotides
Synpcc7942_2179: Synpcc7942_2179 - putative lipid kinase, at 2,258,445 to 2,259,359
_2179
Synpcc7942_2180: prxQ-A1 - bacterioferritin comigratory protein, at 2,259,490 to 2,259,927
prxQ-A1
Synpcc7942_2181: Synpcc7942_2181 - hypothetical protein, at 2,259,980 to 2,261,191
_2181
Synpcc7942_2182: Synpcc7942_2182 - hypothetical protein, at 2,261,244 to 2,261,429
_2182
Synpcc7942_2183: rnhA - RNase HI, at 2,261,556 to 2,262,335
rnhA
Position (kb)
2259
2260
2261
2262 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2258.982 kb on - strand, within Synpcc7942_2179 at 2258.997 kb on + strand, within Synpcc7942_2179 at 2259.005 kb on - strand, within Synpcc7942_2179 at 2259.009 kb on + strand, within Synpcc7942_2179 at 2259.009 kb on + strand, within Synpcc7942_2179 at 2259.009 kb on + strand, within Synpcc7942_2179 at 2259.036 kb on - strand, within Synpcc7942_2179 at 2259.036 kb on - strand, within Synpcc7942_2179 at 2259.057 kb on - strand, within Synpcc7942_2179 at 2259.060 kb on + strand, within Synpcc7942_2179 at 2259.063 kb on - strand, within Synpcc7942_2179 at 2259.125 kb on + strand, within Synpcc7942_2179 at 2259.127 kb on - strand, within Synpcc7942_2179 at 2259.128 kb on - strand, within Synpcc7942_2179 at 2259.128 kb on - strand, within Synpcc7942_2179 at 2259.128 kb on - strand, within Synpcc7942_2179 at 2259.128 kb on - strand, within Synpcc7942_2179 at 2259.141 kb on - strand, within Synpcc7942_2179 at 2259.151 kb on - strand, within Synpcc7942_2179 at 2259.188 kb on + strand, within Synpcc7942_2179 at 2259.193 kb on + strand, within Synpcc7942_2179 at 2259.226 kb on + strand, within Synpcc7942_2179 at 2259.231 kb on + strand at 2259.285 kb on - strand at 2259.287 kb on + strand at 2259.287 kb on + strand at 2259.287 kb on + strand at 2259.287 kb on + strand at 2259.287 kb on + strand at 2259.295 kb on - strand at 2259.295 kb on - strand at 2259.295 kb on - strand at 2259.295 kb on - strand at 2259.326 kb on + strand at 2259.336 kb on + strand at 2259.339 kb on - strand at 2259.344 kb on - strand at 2259.363 kb on + strand at 2259.400 kb on - strand at 2259.424 kb on - strand at 2259.436 kb on - strand at 2259.451 kb on + strand at 2259.468 kb on - strand at 2259.475 kb on + strand at 2259.475 kb on + strand at 2259.503 kb on - strand at 2259.544 kb on + strand, within prxQ-A1 at 2259.544 kb on + strand, within prxQ-A1 at 2259.544 kb on + strand, within prxQ-A1 at 2259.588 kb on - strand, within prxQ-A1 at 2259.604 kb on + strand, within prxQ-A1 at 2259.618 kb on - strand, within prxQ-A1 at 2259.620 kb on + strand, within prxQ-A1 at 2259.626 kb on + strand, within prxQ-A1 at 2259.628 kb on - strand, within prxQ-A1 at 2259.628 kb on - strand, within prxQ-A1 at 2259.719 kb on - strand, within prxQ-A1 at 2259.733 kb on + strand, within prxQ-A1 at 2259.739 kb on + strand, within prxQ-A1 at 2259.739 kb on + strand, within prxQ-A1 at 2259.739 kb on + strand, within prxQ-A1 at 2259.784 kb on - strand, within prxQ-A1 at 2259.795 kb on + strand, within prxQ-A1 at 2259.795 kb on + strand, within prxQ-A1 at 2259.795 kb on + strand, within prxQ-A1 at 2259.828 kb on - strand, within prxQ-A1 at 2259.873 kb on - strand, within prxQ-A1 at 2259.873 kb on - strand, within prxQ-A1 at 2259.873 kb on - strand, within prxQ-A1 at 2259.903 kb on + strand at 2259.917 kb on + strand at 2259.917 kb on + strand at 2259.953 kb on + strand at 2259.963 kb on - strand at 2259.972 kb on - strand at 2259.986 kb on + strand at 2259.994 kb on - strand at 2259.994 kb on - strand at 2260.043 kb on + strand at 2260.108 kb on - strand, within Synpcc7942_2181 at 2260.151 kb on + strand, within Synpcc7942_2181 at 2260.209 kb on + strand, within Synpcc7942_2181 at 2260.217 kb on - strand, within Synpcc7942_2181 at 2260.257 kb on - strand, within Synpcc7942_2181 at 2260.410 kb on - strand, within Synpcc7942_2181 at 2260.426 kb on + strand, within Synpcc7942_2181 at 2260.488 kb on - strand, within Synpcc7942_2181 at 2260.546 kb on + strand, within Synpcc7942_2181 at 2260.624 kb on + strand, within Synpcc7942_2181 at 2260.646 kb on - strand, within Synpcc7942_2181 at 2260.648 kb on - strand, within Synpcc7942_2181 at 2260.674 kb on + strand, within Synpcc7942_2181 at 2260.696 kb on + strand, within Synpcc7942_2181 at 2260.696 kb on + strand, within Synpcc7942_2181 at 2260.796 kb on - strand, within Synpcc7942_2181 at 2260.827 kb on + strand, within Synpcc7942_2181 at 2260.845 kb on - strand, within Synpcc7942_2181 at 2260.980 kb on - strand, within Synpcc7942_2181 at 2260.993 kb on - strand, within Synpcc7942_2181 at 2260.997 kb on + strand, within Synpcc7942_2181 at 2260.997 kb on + strand, within Synpcc7942_2181 at 2261.005 kb on - strand, within Synpcc7942_2181 at 2261.024 kb on + strand, within Synpcc7942_2181 at 2261.051 kb on - strand, within Synpcc7942_2181 at 2261.051 kb on - strand, within Synpcc7942_2181 at 2261.096 kb on - strand at 2261.145 kb on - strand at 2261.164 kb on - strand at 2261.183 kb on + strand at 2261.187 kb on + strand at 2261.194 kb on + strand at 2261.256 kb on + strand at 2261.341 kb on + strand, within Synpcc7942_2182 at 2261.348 kb on + strand, within Synpcc7942_2182 at 2261.356 kb on - strand, within Synpcc7942_2182 at 2261.431 kb on + strand at 2261.431 kb on + strand at 2261.433 kb on + strand at 2261.519 kb on + strand at 2261.527 kb on - strand at 2261.527 kb on - strand at 2261.537 kb on - strand at 2261.553 kb on + strand at 2261.555 kb on + strand at 2261.561 kb on - strand at 2261.593 kb on + strand at 2261.612 kb on + strand at 2261.615 kb on + strand at 2261.667 kb on - strand, within rnhA at 2261.674 kb on + strand, within rnhA at 2261.675 kb on + strand, within rnhA at 2261.681 kb on + strand, within rnhA at 2261.698 kb on - strand, within rnhA at 2261.698 kb on - strand, within rnhA at 2261.756 kb on - strand, within rnhA at 2261.773 kb on - strand, within rnhA at 2261.785 kb on + strand, within rnhA at 2261.786 kb on + strand, within rnhA at 2261.791 kb on + strand, within rnhA at 2261.798 kb on + strand, within rnhA at 2261.801 kb on - strand, within rnhA at 2261.801 kb on - strand, within rnhA at 2261.810 kb on + strand, within rnhA at 2261.846 kb on + strand, within rnhA at 2261.908 kb on - strand, within rnhA at 2261.970 kb on + strand, within rnhA at 2261.970 kb on + strand, within rnhA at 2261.978 kb on - strand, within rnhA at 2261.983 kb on + strand, within rnhA at 2261.996 kb on + strand, within rnhA at 2262.024 kb on + strand, within rnhA at 2262.031 kb on + strand, within rnhA at 2262.031 kb on + strand, within rnhA at 2262.063 kb on - strand, within rnhA at 2262.071 kb on + strand, within rnhA at 2262.071 kb on - strand, within rnhA at 2262.077 kb on + strand, within rnhA at 2262.077 kb on + strand, within rnhA at 2262.085 kb on - strand, within rnhA at 2262.085 kb on - strand, within rnhA at 2262.088 kb on - strand, within rnhA at 2262.091 kb on + strand, within rnhA at 2262.115 kb on + strand, within rnhA at 2262.123 kb on - strand, within rnhA at 2262.123 kb on - strand, within rnhA at 2262.125 kb on - strand, within rnhA at 2262.140 kb on - strand, within rnhA at 2262.159 kb on + strand, within rnhA
Per-strain Table
Position Strand Gene LocusTag Fraction BG11 with Cisplatin 0.05 mg/ml remove 2,258,982 - Synpcc7942_2179 0.59 -1.8 2,258,997 + Synpcc7942_2179 0.60 -0.6 2,259,005 - Synpcc7942_2179 0.61 -0.2 2,259,009 + Synpcc7942_2179 0.62 +0.7 2,259,009 + Synpcc7942_2179 0.62 -0.3 2,259,009 + Synpcc7942_2179 0.62 -0.1 2,259,036 - Synpcc7942_2179 0.65 +0.0 2,259,036 - Synpcc7942_2179 0.65 -0.7 2,259,057 - Synpcc7942_2179 0.67 -0.8 2,259,060 + Synpcc7942_2179 0.67 +0.6 2,259,063 - Synpcc7942_2179 0.68 +1.1 2,259,125 + Synpcc7942_2179 0.74 +0.2 2,259,127 - Synpcc7942_2179 0.75 +0.3 2,259,128 - Synpcc7942_2179 0.75 -0.8 2,259,128 - Synpcc7942_2179 0.75 +0.1 2,259,128 - Synpcc7942_2179 0.75 -2.1 2,259,128 - Synpcc7942_2179 0.75 +0.5 2,259,141 - Synpcc7942_2179 0.76 +0.3 2,259,151 - Synpcc7942_2179 0.77 +0.5 2,259,188 + Synpcc7942_2179 0.81 -0.4 2,259,193 + Synpcc7942_2179 0.82 +0.7 2,259,226 + Synpcc7942_2179 0.85 +0.5 2,259,231 + -0.7 2,259,285 - +1.0 2,259,287 + +0.8 2,259,287 + -0.6 2,259,287 + +0.1 2,259,287 + -0.6 2,259,287 + -1.6 2,259,295 - +0.2 2,259,295 - -0.3 2,259,295 - +2.3 2,259,295 - -0.5 2,259,326 + +2.3 2,259,336 + +0.9 2,259,339 - +1.3 2,259,344 - +0.0 2,259,363 + -1.0 2,259,400 - -1.0 2,259,424 - +0.1 2,259,436 - -1.1 2,259,451 + +0.6 2,259,468 - -1.8 2,259,475 + +0.2 2,259,475 + +0.6 2,259,503 - +0.7 2,259,544 + prxQ-A1 Synpcc7942_2180 0.12 -1.7 2,259,544 + prxQ-A1 Synpcc7942_2180 0.12 +0.9 2,259,544 + prxQ-A1 Synpcc7942_2180 0.12 +0.2 2,259,588 - prxQ-A1 Synpcc7942_2180 0.22 +0.4 2,259,604 + prxQ-A1 Synpcc7942_2180 0.26 +0.8 2,259,618 - prxQ-A1 Synpcc7942_2180 0.29 -2.4 2,259,620 + prxQ-A1 Synpcc7942_2180 0.30 -1.6 2,259,626 + prxQ-A1 Synpcc7942_2180 0.31 -1.0 2,259,628 - prxQ-A1 Synpcc7942_2180 0.32 +1.1 2,259,628 - prxQ-A1 Synpcc7942_2180 0.32 +0.2 2,259,719 - prxQ-A1 Synpcc7942_2180 0.52 +0.4 2,259,733 + prxQ-A1 Synpcc7942_2180 0.55 +0.7 2,259,739 + prxQ-A1 Synpcc7942_2180 0.57 -0.1 2,259,739 + prxQ-A1 Synpcc7942_2180 0.57 -0.0 2,259,739 + prxQ-A1 Synpcc7942_2180 0.57 -0.0 2,259,784 - prxQ-A1 Synpcc7942_2180 0.67 -0.1 2,259,795 + prxQ-A1 Synpcc7942_2180 0.70 -0.5 2,259,795 + prxQ-A1 Synpcc7942_2180 0.70 -0.7 2,259,795 + prxQ-A1 Synpcc7942_2180 0.70 +0.6 2,259,828 - prxQ-A1 Synpcc7942_2180 0.77 -0.2 2,259,873 - prxQ-A1 Synpcc7942_2180 0.87 -0.2 2,259,873 - prxQ-A1 Synpcc7942_2180 0.87 -0.0 2,259,873 - prxQ-A1 Synpcc7942_2180 0.87 +0.8 2,259,903 + +0.2 2,259,917 + +0.7 2,259,917 + +0.1 2,259,953 + +0.0 2,259,963 - +0.9 2,259,972 - +0.2 2,259,986 + -1.7 2,259,994 - -2.2 2,259,994 - -2.9 2,260,043 + -1.3 2,260,108 - Synpcc7942_2181 0.11 -0.7 2,260,151 + Synpcc7942_2181 0.14 -1.6 2,260,209 + Synpcc7942_2181 0.19 -3.1 2,260,217 - Synpcc7942_2181 0.20 -3.3 2,260,257 - Synpcc7942_2181 0.23 -3.3 2,260,410 - Synpcc7942_2181 0.35 -1.8 2,260,426 + Synpcc7942_2181 0.37 -1.7 2,260,488 - Synpcc7942_2181 0.42 -2.6 2,260,546 + Synpcc7942_2181 0.47 -1.4 2,260,624 + Synpcc7942_2181 0.53 +0.3 2,260,646 - Synpcc7942_2181 0.55 -2.5 2,260,648 - Synpcc7942_2181 0.55 -0.7 2,260,674 + Synpcc7942_2181 0.57 -1.8 2,260,696 + Synpcc7942_2181 0.59 -0.6 2,260,696 + Synpcc7942_2181 0.59 +0.1 2,260,796 - Synpcc7942_2181 0.67 -2.4 2,260,827 + Synpcc7942_2181 0.70 -3.7 2,260,845 - Synpcc7942_2181 0.71 -3.6 2,260,980 - Synpcc7942_2181 0.83 -3.1 2,260,993 - Synpcc7942_2181 0.84 -2.4 2,260,997 + Synpcc7942_2181 0.84 -1.1 2,260,997 + Synpcc7942_2181 0.84 -2.2 2,261,005 - Synpcc7942_2181 0.85 -0.7 2,261,024 + Synpcc7942_2181 0.86 -1.6 2,261,051 - Synpcc7942_2181 0.88 -3.2 2,261,051 - Synpcc7942_2181 0.88 -3.3 2,261,096 - -2.2 2,261,145 - -3.5 2,261,164 - -0.5 2,261,183 + +0.9 2,261,187 + -1.0 2,261,194 + -0.9 2,261,256 + +0.1 2,261,341 + Synpcc7942_2182 0.52 -1.3 2,261,348 + Synpcc7942_2182 0.56 +0.4 2,261,356 - Synpcc7942_2182 0.60 +0.1 2,261,431 + +0.2 2,261,431 + -0.6 2,261,433 + +0.4 2,261,519 + +1.6 2,261,527 - -1.2 2,261,527 - +0.2 2,261,537 - +1.3 2,261,553 + +0.9 2,261,555 + -1.8 2,261,561 - -0.1 2,261,593 + -0.1 2,261,612 + +0.1 2,261,615 + +0.2 2,261,667 - rnhA Synpcc7942_2183 0.14 -0.2 2,261,674 + rnhA Synpcc7942_2183 0.15 +0.6 2,261,675 + rnhA Synpcc7942_2183 0.15 -0.2 2,261,681 + rnhA Synpcc7942_2183 0.16 +0.3 2,261,698 - rnhA Synpcc7942_2183 0.18 +0.1 2,261,698 - rnhA Synpcc7942_2183 0.18 +0.2 2,261,756 - rnhA Synpcc7942_2183 0.26 -0.7 2,261,773 - rnhA Synpcc7942_2183 0.28 -0.1 2,261,785 + rnhA Synpcc7942_2183 0.29 -0.3 2,261,786 + rnhA Synpcc7942_2183 0.29 +0.8 2,261,791 + rnhA Synpcc7942_2183 0.30 +0.2 2,261,798 + rnhA Synpcc7942_2183 0.31 +0.5 2,261,801 - rnhA Synpcc7942_2183 0.31 -1.7 2,261,801 - rnhA Synpcc7942_2183 0.31 +0.7 2,261,810 + rnhA Synpcc7942_2183 0.33 +0.3 2,261,846 + rnhA Synpcc7942_2183 0.37 +1.5 2,261,908 - rnhA Synpcc7942_2183 0.45 -1.1 2,261,970 + rnhA Synpcc7942_2183 0.53 +0.0 2,261,970 + rnhA Synpcc7942_2183 0.53 -0.9 2,261,978 - rnhA Synpcc7942_2183 0.54 +0.2 2,261,983 + rnhA Synpcc7942_2183 0.55 -0.9 2,261,996 + rnhA Synpcc7942_2183 0.56 -0.2 2,262,024 + rnhA Synpcc7942_2183 0.60 -1.2 2,262,031 + rnhA Synpcc7942_2183 0.61 -0.9 2,262,031 + rnhA Synpcc7942_2183 0.61 -0.4 2,262,063 - rnhA Synpcc7942_2183 0.65 -1.5 2,262,071 + rnhA Synpcc7942_2183 0.66 -0.8 2,262,071 - rnhA Synpcc7942_2183 0.66 +0.4 2,262,077 + rnhA Synpcc7942_2183 0.67 +0.8 2,262,077 + rnhA Synpcc7942_2183 0.67 +0.4 2,262,085 - rnhA Synpcc7942_2183 0.68 +0.6 2,262,085 - rnhA Synpcc7942_2183 0.68 +1.0 2,262,088 - rnhA Synpcc7942_2183 0.68 -0.5 2,262,091 + rnhA Synpcc7942_2183 0.69 +0.0 2,262,115 + rnhA Synpcc7942_2183 0.72 -0.9 2,262,123 - rnhA Synpcc7942_2183 0.73 +0.0 2,262,123 - rnhA Synpcc7942_2183 0.73 +0.3 2,262,125 - rnhA Synpcc7942_2183 0.73 -0.8 2,262,140 - rnhA Synpcc7942_2183 0.75 -1.1 2,262,159 + rnhA Synpcc7942_2183 0.77 -1.9
Or see this region's nucleotide sequence