Experiment: BG11 with Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt acsF and Synpcc7942_1908 are separated by 153 nucleotides Synpcc7942_1908 and Synpcc7942_1909 are separated by 60 nucleotides Synpcc7942_1909 and ubiX are separated by 2 nucleotides ubiX and zam overlap by 4 nucleotides
Synpcc7942_1907: acsF - Mg-protoporphyrin IX monomethyl ester (oxidative) cyclase, at 1,983,417 to 1,984,493
acsF
Synpcc7942_1908: Synpcc7942_1908 - hypothetical protein, at 1,984,647 to 1,985,081
_1908
Synpcc7942_1909: Synpcc7942_1909 - hypothetical protein, at 1,985,142 to 1,985,738
_1909
Synpcc7942_1910: ubiX - aromatic acid decarboxylase, at 1,985,741 to 1,986,379
ubiX
Synpcc7942_1911: zam - Cold shock protein, at 1,986,376 to 1,988,613
zam
Position (kb)
1985
1986 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1984.660 kb on + strand at 1984.664 kb on + strand at 1984.668 kb on - strand at 1984.689 kb on - strand at 1984.717 kb on - strand, within Synpcc7942_1908 at 1984.742 kb on + strand, within Synpcc7942_1908 at 1984.752 kb on + strand, within Synpcc7942_1908 at 1984.787 kb on + strand, within Synpcc7942_1908 at 1984.805 kb on - strand, within Synpcc7942_1908 at 1984.806 kb on - strand, within Synpcc7942_1908 at 1984.887 kb on - strand, within Synpcc7942_1908 at 1984.887 kb on - strand, within Synpcc7942_1908 at 1984.938 kb on + strand, within Synpcc7942_1908 at 1984.946 kb on - strand, within Synpcc7942_1908 at 1984.947 kb on - strand, within Synpcc7942_1908 at 1984.965 kb on - strand, within Synpcc7942_1908 at 1984.965 kb on - strand, within Synpcc7942_1908 at 1985.035 kb on + strand, within Synpcc7942_1908 at 1985.072 kb on - strand at 1985.092 kb on - strand at 1985.092 kb on - strand at 1985.110 kb on + strand at 1985.113 kb on - strand at 1985.113 kb on - strand at 1985.115 kb on + strand at 1985.123 kb on - strand at 1985.123 kb on - strand at 1985.129 kb on - strand at 1985.227 kb on - strand, within Synpcc7942_1909 at 1985.733 kb on + strand at 1985.741 kb on - strand at 1985.746 kb on - strand at 1985.763 kb on - strand at 1985.763 kb on - strand at 1986.377 kb on - strand at 1986.385 kb on + strand at 1986.385 kb on + strand at 1986.385 kb on + strand at 1986.385 kb on + strand at 1986.393 kb on - strand at 1986.393 kb on - strand at 1986.393 kb on - strand at 1986.430 kb on + strand at 1986.614 kb on + strand, within zam at 1986.622 kb on - strand, within zam at 1986.730 kb on + strand, within zam
Per-strain Table
Position Strand Gene LocusTag Fraction BG11 with Cisplatin 0.05 mg/ml remove 1,984,660 + -0.4 1,984,664 + -1.6 1,984,668 - -0.0 1,984,689 - -0.3 1,984,717 - Synpcc7942_1908 0.16 +0.8 1,984,742 + Synpcc7942_1908 0.22 -0.2 1,984,752 + Synpcc7942_1908 0.24 -0.5 1,984,787 + Synpcc7942_1908 0.32 +0.6 1,984,805 - Synpcc7942_1908 0.36 -1.1 1,984,806 - Synpcc7942_1908 0.37 +0.3 1,984,887 - Synpcc7942_1908 0.55 +0.9 1,984,887 - Synpcc7942_1908 0.55 -0.0 1,984,938 + Synpcc7942_1908 0.67 -0.1 1,984,946 - Synpcc7942_1908 0.69 -0.2 1,984,947 - Synpcc7942_1908 0.69 +0.2 1,984,965 - Synpcc7942_1908 0.73 +0.4 1,984,965 - Synpcc7942_1908 0.73 -0.2 1,985,035 + Synpcc7942_1908 0.89 +1.1 1,985,072 - -0.2 1,985,092 - -2.2 1,985,092 - -0.9 1,985,110 + +0.3 1,985,113 - +0.0 1,985,113 - -0.1 1,985,115 + -0.7 1,985,123 - -0.7 1,985,123 - +0.2 1,985,129 - +1.2 1,985,227 - Synpcc7942_1909 0.14 -3.0 1,985,733 + -0.1 1,985,741 - -0.8 1,985,746 - -0.1 1,985,763 - +0.4 1,985,763 - +1.0 1,986,377 - -0.8 1,986,385 + -0.3 1,986,385 + -1.8 1,986,385 + -3.8 1,986,385 + -1.4 1,986,393 - -2.1 1,986,393 - -1.1 1,986,393 - -3.9 1,986,430 + -2.6 1,986,614 + zam Synpcc7942_1911 0.11 -0.5 1,986,622 - zam Synpcc7942_1911 0.11 -1.6 1,986,730 + zam Synpcc7942_1911 0.16 -3.9
Or see this region's nucleotide sequence