Experiment: BG11 with Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rfaE and Synpcc7942_1885 overlap by 4 nucleotides Synpcc7942_1885 and recJ are separated by 5 nucleotides recJ and Synpcc7942_1887 are separated by 52 nucleotides
Synpcc7942_1884: rfaE - RfaE bifunctional protein, domain II, at 1,956,126 to 1,956,647
rfaE
Synpcc7942_1885: Synpcc7942_1885 - HAD-superfamily hydrolase subfamily IA, variant 3, at 1,956,644 to 1,957,411
_1885
Synpcc7942_1886: recJ - exonuclease RecJ, at 1,957,417 to 1,959,471
recJ
Synpcc7942_1887: Synpcc7942_1887 - probable mannosyltransferase, at 1,959,524 to 1,960,816
_1887
Position (kb)
1957
1958
1959
1960 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1956.640 kb on + strand at 1956.648 kb on - strand at 1956.655 kb on - strand at 1956.675 kb on + strand at 1956.675 kb on - strand at 1956.689 kb on + strand at 1956.699 kb on + strand at 1956.703 kb on + strand at 1956.716 kb on + strand at 1956.721 kb on + strand, within Synpcc7942_1885 at 1956.736 kb on - strand, within Synpcc7942_1885 at 1956.760 kb on - strand, within Synpcc7942_1885 at 1956.794 kb on + strand, within Synpcc7942_1885 at 1956.795 kb on + strand, within Synpcc7942_1885 at 1956.803 kb on - strand, within Synpcc7942_1885 at 1956.834 kb on + strand, within Synpcc7942_1885 at 1957.066 kb on - strand, within Synpcc7942_1885 at 1957.068 kb on + strand, within Synpcc7942_1885 at 1957.176 kb on + strand, within Synpcc7942_1885 at 1957.301 kb on + strand, within Synpcc7942_1885 at 1957.301 kb on + strand, within Synpcc7942_1885 at 1957.301 kb on + strand, within Synpcc7942_1885 at 1957.301 kb on + strand, within Synpcc7942_1885 at 1957.359 kb on + strand at 1957.425 kb on + strand at 1957.427 kb on + strand at 1957.427 kb on + strand at 1957.433 kb on - strand at 1957.488 kb on + strand at 1957.504 kb on + strand at 1957.511 kb on + strand at 1957.511 kb on - strand at 1957.548 kb on - strand at 1957.623 kb on + strand, within recJ at 1957.631 kb on - strand, within recJ at 1957.762 kb on + strand, within recJ at 1957.762 kb on + strand, within recJ at 1957.780 kb on - strand, within recJ at 1957.793 kb on + strand, within recJ at 1957.801 kb on - strand, within recJ at 1957.827 kb on - strand, within recJ at 1957.879 kb on + strand at 1957.881 kb on - strand, within recJ at 1957.914 kb on - strand, within recJ at 1958.080 kb on - strand, within recJ at 1958.099 kb on + strand, within recJ at 1958.099 kb on + strand, within recJ at 1958.099 kb on + strand, within recJ at 1958.163 kb on + strand at 1958.164 kb on + strand, within recJ at 1958.171 kb on - strand, within recJ at 1958.222 kb on + strand, within recJ at 1958.287 kb on - strand, within recJ at 1958.350 kb on + strand, within recJ at 1958.514 kb on + strand, within recJ at 1958.520 kb on + strand, within recJ at 1958.536 kb on - strand at 1958.556 kb on + strand, within recJ at 1958.594 kb on + strand, within recJ at 1958.604 kb on - strand, within recJ at 1958.614 kb on + strand, within recJ at 1958.749 kb on + strand, within recJ at 1958.753 kb on + strand at 1958.772 kb on - strand, within recJ at 1958.821 kb on - strand, within recJ at 1958.920 kb on - strand, within recJ at 1959.054 kb on + strand, within recJ at 1959.054 kb on + strand at 1959.130 kb on - strand, within recJ at 1959.244 kb on - strand, within recJ at 1959.348 kb on + strand at 1959.420 kb on - strand at 1959.539 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BG11 with Cisplatin 0.05 mg/ml remove 1,956,640 + -0.4 1,956,648 - +1.3 1,956,655 - -1.3 1,956,675 + -0.1 1,956,675 - +1.7 1,956,689 + +1.1 1,956,699 + -0.3 1,956,703 + +1.2 1,956,716 + -1.1 1,956,721 + Synpcc7942_1885 0.10 -0.1 1,956,736 - Synpcc7942_1885 0.12 -2.3 1,956,760 - Synpcc7942_1885 0.15 -0.8 1,956,794 + Synpcc7942_1885 0.20 +0.1 1,956,795 + Synpcc7942_1885 0.20 -0.5 1,956,803 - Synpcc7942_1885 0.21 +1.5 1,956,834 + Synpcc7942_1885 0.25 -0.4 1,957,066 - Synpcc7942_1885 0.55 +0.8 1,957,068 + Synpcc7942_1885 0.55 +0.2 1,957,176 + Synpcc7942_1885 0.69 -1.0 1,957,301 + Synpcc7942_1885 0.86 -1.5 1,957,301 + Synpcc7942_1885 0.86 +0.3 1,957,301 + Synpcc7942_1885 0.86 +0.2 1,957,301 + Synpcc7942_1885 0.86 -0.6 1,957,359 + -1.9 1,957,425 + +0.5 1,957,427 + -0.3 1,957,427 + +0.5 1,957,433 - -1.9 1,957,488 + -1.3 1,957,504 + -2.6 1,957,511 + -1.6 1,957,511 - -2.2 1,957,548 - +0.6 1,957,623 + recJ Synpcc7942_1886 0.10 -0.9 1,957,631 - recJ Synpcc7942_1886 0.10 -2.9 1,957,762 + recJ Synpcc7942_1886 0.17 -0.9 1,957,762 + recJ Synpcc7942_1886 0.17 +0.8 1,957,780 - recJ Synpcc7942_1886 0.18 -1.5 1,957,793 + recJ Synpcc7942_1886 0.18 -2.5 1,957,801 - recJ Synpcc7942_1886 0.19 +0.9 1,957,827 - recJ Synpcc7942_1886 0.20 +0.5 1,957,879 + +0.1 1,957,881 - recJ Synpcc7942_1886 0.23 -0.8 1,957,914 - recJ Synpcc7942_1886 0.24 -1.8 1,958,080 - recJ Synpcc7942_1886 0.32 -0.5 1,958,099 + recJ Synpcc7942_1886 0.33 -1.6 1,958,099 + recJ Synpcc7942_1886 0.33 -0.3 1,958,099 + recJ Synpcc7942_1886 0.33 -0.1 1,958,163 + -0.3 1,958,164 + recJ Synpcc7942_1886 0.36 -1.2 1,958,171 - recJ Synpcc7942_1886 0.37 -1.4 1,958,222 + recJ Synpcc7942_1886 0.39 -1.2 1,958,287 - recJ Synpcc7942_1886 0.42 -2.3 1,958,350 + recJ Synpcc7942_1886 0.45 -0.5 1,958,514 + recJ Synpcc7942_1886 0.53 -2.6 1,958,520 + recJ Synpcc7942_1886 0.54 -1.1 1,958,536 - -0.3 1,958,556 + recJ Synpcc7942_1886 0.55 +0.5 1,958,594 + recJ Synpcc7942_1886 0.57 -1.3 1,958,604 - recJ Synpcc7942_1886 0.58 -2.1 1,958,614 + recJ Synpcc7942_1886 0.58 -1.5 1,958,749 + recJ Synpcc7942_1886 0.65 -1.8 1,958,753 + -0.8 1,958,772 - recJ Synpcc7942_1886 0.66 -2.6 1,958,821 - recJ Synpcc7942_1886 0.68 -0.4 1,958,920 - recJ Synpcc7942_1886 0.73 -1.5 1,959,054 + recJ Synpcc7942_1886 0.80 -1.1 1,959,054 + -0.8 1,959,130 - recJ Synpcc7942_1886 0.83 -1.6 1,959,244 - recJ Synpcc7942_1886 0.89 -1.6 1,959,348 + -1.5 1,959,420 - -0.9 1,959,539 + -0.8
Or see this region's nucleotide sequence