Experiment: BG11 with Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_1709 and rpoZ are separated by 158 nucleotides rpoZ and Synpcc7942_1711 overlap by 4 nucleotides Synpcc7942_1711 and Synpcc7942_1712 are separated by 5 nucleotides
Synpcc7942_1709: Synpcc7942_1709 - Small GTP-binding protein domain, at 1,778,348 to 1,779,889
_1709
Synpcc7942_1710: rpoZ - DNA-directed RNA polymerase subunit omega, at 1,780,048 to 1,780,278
rpoZ
Synpcc7942_1711: Synpcc7942_1711 - hypothetical protein, at 1,780,275 to 1,780,847
_1711
Synpcc7942_1712: Synpcc7942_1712 - hypothetical protein, at 1,780,853 to 1,781,218
_1712
Position (kb)
1780
1781 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1779.894 kb on - strand at 1779.913 kb on + strand at 1779.914 kb on - strand at 1779.916 kb on + strand at 1779.916 kb on + strand at 1779.921 kb on - strand at 1779.949 kb on + strand at 1779.959 kb on + strand at 1779.959 kb on + strand at 1779.959 kb on + strand at 1779.959 kb on + strand at 1779.959 kb on + strand at 1779.959 kb on + strand at 1779.959 kb on + strand at 1779.959 kb on + strand at 1779.964 kb on + strand at 1779.967 kb on - strand at 1779.967 kb on - strand at 1779.967 kb on - strand at 1779.967 kb on - strand at 1779.967 kb on - strand at 1779.967 kb on - strand at 1779.967 kb on - strand at 1779.967 kb on - strand at 1779.967 kb on - strand at 1779.980 kb on + strand at 1779.988 kb on - strand at 1779.988 kb on - strand at 1779.988 kb on - strand at 1780.009 kb on + strand at 1780.013 kb on - strand at 1780.104 kb on + strand, within rpoZ at 1780.167 kb on - strand, within rpoZ at 1780.167 kb on - strand, within rpoZ at 1780.204 kb on + strand, within rpoZ at 1780.214 kb on - strand, within rpoZ at 1780.238 kb on + strand, within rpoZ at 1780.320 kb on + strand at 1780.330 kb on + strand at 1780.333 kb on - strand, within Synpcc7942_1711 at 1780.439 kb on + strand, within Synpcc7942_1711 at 1780.449 kb on + strand, within Synpcc7942_1711 at 1780.450 kb on - strand, within Synpcc7942_1711 at 1780.462 kb on - strand, within Synpcc7942_1711 at 1780.473 kb on - strand at 1780.556 kb on + strand, within Synpcc7942_1711 at 1780.615 kb on + strand, within Synpcc7942_1711 at 1780.624 kb on - strand, within Synpcc7942_1711 at 1780.653 kb on + strand, within Synpcc7942_1711 at 1780.701 kb on + strand, within Synpcc7942_1711 at 1780.769 kb on - strand, within Synpcc7942_1711 at 1780.781 kb on - strand, within Synpcc7942_1711 at 1780.798 kb on - strand at 1780.838 kb on - strand at 1781.128 kb on - strand at 1781.185 kb on + strand at 1781.222 kb on + strand at 1781.228 kb on + strand at 1781.228 kb on + strand at 1781.237 kb on + strand at 1781.237 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BG11 with Cisplatin 0.05 mg/ml remove 1,779,894 - +0.3 1,779,913 + +0.8 1,779,914 - +1.1 1,779,916 + -1.1 1,779,916 + -0.3 1,779,921 - +0.5 1,779,949 + -0.5 1,779,959 + -1.1 1,779,959 + -0.5 1,779,959 + -0.4 1,779,959 + -0.7 1,779,959 + +1.5 1,779,959 + -1.3 1,779,959 + -0.3 1,779,959 + -1.3 1,779,964 + -2.0 1,779,967 - -0.1 1,779,967 - +0.6 1,779,967 - +0.7 1,779,967 - -0.7 1,779,967 - +0.8 1,779,967 - -0.0 1,779,967 - +0.1 1,779,967 - -0.9 1,779,967 - +0.3 1,779,980 + -0.2 1,779,988 - -1.6 1,779,988 - -0.4 1,779,988 - +0.9 1,780,009 + +0.1 1,780,013 - -0.4 1,780,104 + rpoZ Synpcc7942_1710 0.24 -1.4 1,780,167 - rpoZ Synpcc7942_1710 0.52 -1.3 1,780,167 - rpoZ Synpcc7942_1710 0.52 -3.9 1,780,204 + rpoZ Synpcc7942_1710 0.68 -1.5 1,780,214 - rpoZ Synpcc7942_1710 0.72 -1.4 1,780,238 + rpoZ Synpcc7942_1710 0.82 -2.3 1,780,320 + -1.4 1,780,330 + +0.8 1,780,333 - Synpcc7942_1711 0.10 +0.4 1,780,439 + Synpcc7942_1711 0.29 -0.9 1,780,449 + Synpcc7942_1711 0.30 -1.1 1,780,450 - Synpcc7942_1711 0.31 -0.2 1,780,462 - Synpcc7942_1711 0.33 +1.0 1,780,473 - +0.5 1,780,556 + Synpcc7942_1711 0.49 -0.0 1,780,615 + Synpcc7942_1711 0.59 -2.7 1,780,624 - Synpcc7942_1711 0.61 -0.9 1,780,653 + Synpcc7942_1711 0.66 -1.8 1,780,701 + Synpcc7942_1711 0.74 -0.8 1,780,769 - Synpcc7942_1711 0.86 -1.2 1,780,781 - Synpcc7942_1711 0.88 -0.8 1,780,798 - -0.5 1,780,838 - -0.5 1,781,128 - -0.5 1,781,185 + +0.2 1,781,222 + +0.8 1,781,228 + +0.4 1,781,228 + -0.8 1,781,237 + -0.3 1,781,237 + +0.6
Or see this region's nucleotide sequence