Experiment: BG11 with Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cysM and Synpcc7942_1467 are separated by 5 nucleotides Synpcc7942_1467 and Synpcc7942_1468 are separated by 118 nucleotides Synpcc7942_1468 and Synpcc7942_1469 overlap by 4 nucleotides Synpcc7942_1469 and Synpcc7942_1470 overlap by 8 nucleotides Synpcc7942_1470 and Synpcc7942_1471 overlap by 4 nucleotides
Synpcc7942_1466: cysM - cysteine synthase, at 1,519,664 to 1,520,668
cysM
Synpcc7942_1467: Synpcc7942_1467 - Heat shock protein DnaJ-like, at 1,520,674 to 1,521,363
_1467
Synpcc7942_1468: Synpcc7942_1468 - putative monovalent cation/H+ antiporter subunit B, at 1,521,482 to 1,522,141
_1468
Synpcc7942_1469: Synpcc7942_1469 - hypothetical protein, at 1,522,138 to 1,522,719
_1469
Synpcc7942_1470: Synpcc7942_1470 - hypothetical protein, at 1,522,712 to 1,523,005
_1470
Synpcc7942_1471: Synpcc7942_1471 - hypothetical protein, at 1,523,002 to 1,523,259
_1471
Position (kb)
1521
1522
1523 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1 at 1520.500 kb on - strand, within cysM at 1520.595 kb on + strand at 1520.595 kb on + strand at 1520.595 kb on + strand at 1520.608 kb on - strand at 1520.621 kb on + strand at 1520.691 kb on + strand at 1520.763 kb on - strand, within Synpcc7942_1467 at 1520.827 kb on + strand, within Synpcc7942_1467 at 1520.858 kb on + strand, within Synpcc7942_1467 at 1520.858 kb on + strand, within Synpcc7942_1467 at 1520.863 kb on + strand, within Synpcc7942_1467 at 1520.895 kb on + strand, within Synpcc7942_1467 at 1520.897 kb on + strand, within Synpcc7942_1467 at 1520.911 kb on + strand, within Synpcc7942_1467 at 1520.971 kb on - strand, within Synpcc7942_1467 at 1520.971 kb on - strand, within Synpcc7942_1467 at 1520.993 kb on - strand, within Synpcc7942_1467 at 1521.006 kb on - strand, within Synpcc7942_1467 at 1521.038 kb on - strand, within Synpcc7942_1467 at 1521.048 kb on - strand, within Synpcc7942_1467 at 1521.066 kb on - strand, within Synpcc7942_1467 at 1521.069 kb on - strand, within Synpcc7942_1467 at 1521.096 kb on + strand, within Synpcc7942_1467 at 1521.166 kb on + strand, within Synpcc7942_1467 at 1521.213 kb on + strand, within Synpcc7942_1467 at 1521.374 kb on - strand at 1521.376 kb on + strand at 1521.399 kb on + strand at 1521.480 kb on + strand at 1521.584 kb on + strand, within Synpcc7942_1468 at 1521.599 kb on + strand, within Synpcc7942_1468 at 1521.614 kb on - strand, within Synpcc7942_1468 at 1521.795 kb on - strand, within Synpcc7942_1468 at 1522.284 kb on + strand, within Synpcc7942_1469 at 1522.332 kb on - strand, within Synpcc7942_1469 at 1522.332 kb on - strand, within Synpcc7942_1469 at 1522.486 kb on + strand, within Synpcc7942_1469 at 1522.530 kb on - strand, within Synpcc7942_1469 at 1522.541 kb on - strand, within Synpcc7942_1469 at 1522.580 kb on + strand, within Synpcc7942_1469 at 1522.587 kb on - strand, within Synpcc7942_1469 at 1522.587 kb on - strand, within Synpcc7942_1469 at 1522.587 kb on - strand, within Synpcc7942_1469 at 1522.587 kb on - strand, within Synpcc7942_1469 at 1522.587 kb on - strand, within Synpcc7942_1469 at 1522.589 kb on + strand, within Synpcc7942_1469 at 1522.597 kb on - strand, within Synpcc7942_1469 at 1522.597 kb on - strand, within Synpcc7942_1469 at 1522.720 kb on + strand at 1522.784 kb on + strand, within Synpcc7942_1470 at 1522.944 kb on - strand, within Synpcc7942_1470
Per-strain Table
Position Strand Gene LocusTag Fraction BG11 with Cisplatin 0.05 mg/ml remove 1,520,500 - cysM Synpcc7942_1466 0.83 +0.0 1,520,595 + -1.0 1,520,595 + -1.0 1,520,595 + -0.3 1,520,608 - -0.8 1,520,621 + -0.1 1,520,691 + -0.1 1,520,763 - Synpcc7942_1467 0.13 -0.6 1,520,827 + Synpcc7942_1467 0.22 +0.3 1,520,858 + Synpcc7942_1467 0.27 -0.3 1,520,858 + Synpcc7942_1467 0.27 +0.0 1,520,863 + Synpcc7942_1467 0.27 +0.7 1,520,895 + Synpcc7942_1467 0.32 -2.1 1,520,897 + Synpcc7942_1467 0.32 -1.8 1,520,911 + Synpcc7942_1467 0.34 -1.0 1,520,971 - Synpcc7942_1467 0.43 +1.1 1,520,971 - Synpcc7942_1467 0.43 +0.1 1,520,993 - Synpcc7942_1467 0.46 +0.6 1,521,006 - Synpcc7942_1467 0.48 +1.0 1,521,038 - Synpcc7942_1467 0.53 +0.0 1,521,048 - Synpcc7942_1467 0.54 +0.6 1,521,066 - Synpcc7942_1467 0.57 -0.7 1,521,069 - Synpcc7942_1467 0.57 +0.0 1,521,096 + Synpcc7942_1467 0.61 +1.0 1,521,166 + Synpcc7942_1467 0.71 -1.1 1,521,213 + Synpcc7942_1467 0.78 -0.5 1,521,374 - -0.2 1,521,376 + +0.6 1,521,399 + -0.2 1,521,480 + +0.1 1,521,584 + Synpcc7942_1468 0.15 -3.5 1,521,599 + Synpcc7942_1468 0.18 -0.0 1,521,614 - Synpcc7942_1468 0.20 -4.8 1,521,795 - Synpcc7942_1468 0.47 -2.2 1,522,284 + Synpcc7942_1469 0.25 -2.2 1,522,332 - Synpcc7942_1469 0.33 -2.5 1,522,332 - Synpcc7942_1469 0.33 -1.0 1,522,486 + Synpcc7942_1469 0.60 -2.9 1,522,530 - Synpcc7942_1469 0.67 -2.0 1,522,541 - Synpcc7942_1469 0.69 -3.0 1,522,580 + Synpcc7942_1469 0.76 -5.0 1,522,587 - Synpcc7942_1469 0.77 -2.9 1,522,587 - Synpcc7942_1469 0.77 -1.6 1,522,587 - Synpcc7942_1469 0.77 -4.0 1,522,587 - Synpcc7942_1469 0.77 -3.0 1,522,587 - Synpcc7942_1469 0.77 -0.9 1,522,589 + Synpcc7942_1469 0.77 -2.9 1,522,597 - Synpcc7942_1469 0.79 -3.1 1,522,597 - Synpcc7942_1469 0.79 -6.9 1,522,720 + -1.0 1,522,784 + Synpcc7942_1470 0.24 -2.0 1,522,944 - Synpcc7942_1470 0.79 -3.3
Or see this region's nucleotide sequence