Experiment: BG11 with Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_1329 and Synpcc7942_1330 are separated by 2 nucleotides Synpcc7942_1330 and Synpcc7942_1331 are separated by 125 nucleotides Synpcc7942_1331 and Synpcc7942_1332 are separated by 4 nucleotides Synpcc7942_1332 and Synpcc7942_1333 overlap by 1 nucleotides Synpcc7942_1333 and trpE overlap by 4 nucleotides
Synpcc7942_1329: Synpcc7942_1329 - hypothetical protein, at 1,361,879 to 1,362,505
_1329
Synpcc7942_1330: Synpcc7942_1330 - hypothetical protein, at 1,362,508 to 1,363,164
_1330
Synpcc7942_1331: Synpcc7942_1331 - CAB/ELIP/HLIP superfamily protein, at 1,363,290 to 1,363,466
_1331
Synpcc7942_1332: Synpcc7942_1332 - hypothetical protein, at 1,363,471 to 1,364,091
_1332
Synpcc7942_1333: Synpcc7942_1333 - ExsB, at 1,364,091 to 1,364,786
_1333
Synpcc7942_1334: trpE - aminodeoxychorismate synthase, subunit I, at 1,364,783 to 1,366,180
trpE
Position (kb)
1363
1364
1365 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1362.487 kb on + strand at 1362.490 kb on - strand at 1362.497 kb on - strand at 1362.533 kb on + strand at 1362.535 kb on + strand at 1362.541 kb on - strand at 1362.543 kb on - strand at 1362.551 kb on - strand at 1362.576 kb on + strand, within Synpcc7942_1330 at 1362.576 kb on + strand, within Synpcc7942_1330 at 1362.579 kb on - strand at 1362.583 kb on - strand, within Synpcc7942_1330 at 1362.584 kb on - strand, within Synpcc7942_1330 at 1362.584 kb on - strand, within Synpcc7942_1330 at 1362.584 kb on - strand, within Synpcc7942_1330 at 1362.584 kb on - strand, within Synpcc7942_1330 at 1362.629 kb on + strand, within Synpcc7942_1330 at 1362.637 kb on - strand, within Synpcc7942_1330 at 1362.660 kb on + strand, within Synpcc7942_1330 at 1362.665 kb on + strand, within Synpcc7942_1330 at 1362.666 kb on + strand, within Synpcc7942_1330 at 1362.743 kb on - strand, within Synpcc7942_1330 at 1362.745 kb on + strand, within Synpcc7942_1330 at 1362.804 kb on - strand, within Synpcc7942_1330 at 1362.835 kb on + strand, within Synpcc7942_1330 at 1362.843 kb on + strand, within Synpcc7942_1330 at 1362.855 kb on + strand, within Synpcc7942_1330 at 1362.865 kb on + strand, within Synpcc7942_1330 at 1362.886 kb on - strand, within Synpcc7942_1330 at 1362.886 kb on - strand, within Synpcc7942_1330 at 1362.897 kb on + strand, within Synpcc7942_1330 at 1362.905 kb on - strand, within Synpcc7942_1330 at 1362.905 kb on - strand, within Synpcc7942_1330 at 1362.905 kb on - strand, within Synpcc7942_1330 at 1362.907 kb on + strand, within Synpcc7942_1330 at 1362.909 kb on + strand, within Synpcc7942_1330 at 1362.909 kb on + strand, within Synpcc7942_1330 at 1362.909 kb on + strand, within Synpcc7942_1330 at 1362.909 kb on + strand, within Synpcc7942_1330 at 1362.909 kb on + strand, within Synpcc7942_1330 at 1362.917 kb on - strand, within Synpcc7942_1330 at 1362.917 kb on - strand, within Synpcc7942_1330 at 1362.974 kb on - strand, within Synpcc7942_1330 at 1362.996 kb on - strand, within Synpcc7942_1330 at 1363.022 kb on - strand, within Synpcc7942_1330 at 1363.022 kb on - strand, within Synpcc7942_1330 at 1363.032 kb on + strand, within Synpcc7942_1330 at 1363.032 kb on + strand, within Synpcc7942_1330 at 1363.040 kb on - strand, within Synpcc7942_1330 at 1363.040 kb on - strand, within Synpcc7942_1330 at 1363.064 kb on + strand, within Synpcc7942_1330 at 1363.084 kb on + strand, within Synpcc7942_1330 at 1363.113 kb on + strand at 1363.134 kb on + strand at 1363.142 kb on - strand at 1363.149 kb on + strand at 1363.157 kb on - strand at 1363.157 kb on - strand at 1363.157 kb on - strand at 1363.157 kb on - strand at 1363.166 kb on - strand at 1363.167 kb on - strand at 1363.212 kb on - strand at 1363.250 kb on + strand at 1363.354 kb on + strand, within Synpcc7942_1331 at 1363.362 kb on - strand, within Synpcc7942_1331 at 1363.402 kb on + strand, within Synpcc7942_1331 at 1363.467 kb on + strand at 1363.469 kb on - strand at 1363.475 kb on - strand at 1363.485 kb on - strand at 1363.485 kb on - strand at 1363.490 kb on - strand at 1363.531 kb on + strand at 1363.539 kb on - strand, within Synpcc7942_1332 at 1363.552 kb on - strand, within Synpcc7942_1332 at 1363.557 kb on + strand, within Synpcc7942_1332 at 1363.589 kb on + strand, within Synpcc7942_1332 at 1363.597 kb on - strand, within Synpcc7942_1332 at 1363.616 kb on - strand, within Synpcc7942_1332 at 1363.631 kb on - strand, within Synpcc7942_1332 at 1363.668 kb on + strand, within Synpcc7942_1332 at 1363.681 kb on + strand, within Synpcc7942_1332 at 1363.692 kb on + strand, within Synpcc7942_1332 at 1363.692 kb on + strand, within Synpcc7942_1332 at 1363.700 kb on - strand, within Synpcc7942_1332 at 1363.749 kb on + strand, within Synpcc7942_1332 at 1363.770 kb on + strand, within Synpcc7942_1332 at 1363.770 kb on + strand, within Synpcc7942_1332 at 1363.771 kb on + strand, within Synpcc7942_1332 at 1363.779 kb on - strand, within Synpcc7942_1332 at 1363.802 kb on - strand, within Synpcc7942_1332 at 1363.829 kb on + strand, within Synpcc7942_1332 at 1363.873 kb on + strand, within Synpcc7942_1332 at 1363.881 kb on - strand, within Synpcc7942_1332 at 1363.938 kb on + strand, within Synpcc7942_1332 at 1363.942 kb on + strand, within Synpcc7942_1332 at 1363.985 kb on - strand, within Synpcc7942_1332 at 1363.985 kb on - strand, within Synpcc7942_1332 at 1363.989 kb on + strand, within Synpcc7942_1332 at 1364.108 kb on - strand at 1364.131 kb on - strand at 1364.145 kb on + strand at 1364.172 kb on - strand, within Synpcc7942_1333 at 1364.189 kb on + strand, within Synpcc7942_1333 at 1364.204 kb on - strand, within Synpcc7942_1333 at 1364.259 kb on - strand, within Synpcc7942_1333 at 1364.307 kb on - strand, within Synpcc7942_1333 at 1364.312 kb on - strand, within Synpcc7942_1333 at 1364.465 kb on + strand, within Synpcc7942_1333 at 1364.553 kb on + strand, within Synpcc7942_1333 at 1364.561 kb on - strand, within Synpcc7942_1333 at 1364.630 kb on - strand, within Synpcc7942_1333 at 1364.676 kb on - strand, within Synpcc7942_1333 at 1365.024 kb on + strand, within trpE
Per-strain Table
Position Strand Gene LocusTag Fraction BG11 with Cisplatin 0.05 mg/ml remove 1,362,487 + +0.7 1,362,490 - -0.2 1,362,497 - +1.1 1,362,533 + -1.1 1,362,535 + -1.0 1,362,541 - +0.5 1,362,543 - +0.7 1,362,551 - +0.6 1,362,576 + Synpcc7942_1330 0.10 -0.4 1,362,576 + Synpcc7942_1330 0.10 -0.4 1,362,579 - +0.5 1,362,583 - Synpcc7942_1330 0.11 +1.1 1,362,584 - Synpcc7942_1330 0.12 +0.0 1,362,584 - Synpcc7942_1330 0.12 -1.4 1,362,584 - Synpcc7942_1330 0.12 +0.0 1,362,584 - Synpcc7942_1330 0.12 -0.8 1,362,629 + Synpcc7942_1330 0.18 +1.2 1,362,637 - Synpcc7942_1330 0.20 +0.3 1,362,660 + Synpcc7942_1330 0.23 +0.4 1,362,665 + Synpcc7942_1330 0.24 +0.2 1,362,666 + Synpcc7942_1330 0.24 -1.3 1,362,743 - Synpcc7942_1330 0.36 -0.4 1,362,745 + Synpcc7942_1330 0.36 +0.2 1,362,804 - Synpcc7942_1330 0.45 +1.6 1,362,835 + Synpcc7942_1330 0.50 -0.3 1,362,843 + Synpcc7942_1330 0.51 -0.6 1,362,855 + Synpcc7942_1330 0.53 +0.2 1,362,865 + Synpcc7942_1330 0.54 -0.1 1,362,886 - Synpcc7942_1330 0.58 +0.4 1,362,886 - Synpcc7942_1330 0.58 -0.8 1,362,897 + Synpcc7942_1330 0.59 -0.1 1,362,905 - Synpcc7942_1330 0.60 +0.0 1,362,905 - Synpcc7942_1330 0.60 +1.6 1,362,905 - Synpcc7942_1330 0.60 -1.0 1,362,907 + Synpcc7942_1330 0.61 +0.1 1,362,909 + Synpcc7942_1330 0.61 -0.4 1,362,909 + Synpcc7942_1330 0.61 -1.2 1,362,909 + Synpcc7942_1330 0.61 +0.1 1,362,909 + Synpcc7942_1330 0.61 -0.6 1,362,909 + Synpcc7942_1330 0.61 -0.8 1,362,917 - Synpcc7942_1330 0.62 +0.1 1,362,917 - Synpcc7942_1330 0.62 -1.8 1,362,974 - Synpcc7942_1330 0.71 +0.4 1,362,996 - Synpcc7942_1330 0.74 -3.5 1,363,022 - Synpcc7942_1330 0.78 +0.9 1,363,022 - Synpcc7942_1330 0.78 -1.4 1,363,032 + Synpcc7942_1330 0.80 -0.4 1,363,032 + Synpcc7942_1330 0.80 -1.7 1,363,040 - Synpcc7942_1330 0.81 +1.3 1,363,040 - Synpcc7942_1330 0.81 -2.3 1,363,064 + Synpcc7942_1330 0.85 +0.3 1,363,084 + Synpcc7942_1330 0.88 -1.3 1,363,113 + -0.9 1,363,134 + +0.1 1,363,142 - -1.0 1,363,149 + -0.4 1,363,157 - -0.0 1,363,157 - -0.5 1,363,157 - -0.7 1,363,157 - -1.5 1,363,166 - +0.4 1,363,167 - -2.2 1,363,212 - -0.9 1,363,250 + +0.4 1,363,354 + Synpcc7942_1331 0.36 -1.9 1,363,362 - Synpcc7942_1331 0.41 -1.6 1,363,402 + Synpcc7942_1331 0.63 -0.0 1,363,467 + -1.4 1,363,469 - -0.3 1,363,475 - -0.3 1,363,485 - +0.4 1,363,485 - -1.1 1,363,490 - -2.0 1,363,531 + -1.5 1,363,539 - Synpcc7942_1332 0.11 -3.9 1,363,552 - Synpcc7942_1332 0.13 -0.9 1,363,557 + Synpcc7942_1332 0.14 -2.6 1,363,589 + Synpcc7942_1332 0.19 -2.3 1,363,597 - Synpcc7942_1332 0.20 -3.1 1,363,616 - Synpcc7942_1332 0.23 -2.6 1,363,631 - Synpcc7942_1332 0.26 -1.4 1,363,668 + Synpcc7942_1332 0.32 -1.5 1,363,681 + Synpcc7942_1332 0.34 -3.9 1,363,692 + Synpcc7942_1332 0.36 -1.7 1,363,692 + Synpcc7942_1332 0.36 -1.6 1,363,700 - Synpcc7942_1332 0.37 -1.9 1,363,749 + Synpcc7942_1332 0.45 -4.0 1,363,770 + Synpcc7942_1332 0.48 -0.7 1,363,770 + Synpcc7942_1332 0.48 -3.9 1,363,771 + Synpcc7942_1332 0.48 -0.5 1,363,779 - Synpcc7942_1332 0.50 -2.3 1,363,802 - Synpcc7942_1332 0.53 -0.8 1,363,829 + Synpcc7942_1332 0.58 -2.7 1,363,873 + Synpcc7942_1332 0.65 -1.6 1,363,881 - Synpcc7942_1332 0.66 -1.1 1,363,938 + Synpcc7942_1332 0.75 -3.0 1,363,942 + Synpcc7942_1332 0.76 -2.1 1,363,985 - Synpcc7942_1332 0.83 -2.7 1,363,985 - Synpcc7942_1332 0.83 -3.1 1,363,989 + Synpcc7942_1332 0.83 -2.3 1,364,108 - -2.2 1,364,131 - -2.2 1,364,145 + -2.0 1,364,172 - Synpcc7942_1333 0.12 -0.4 1,364,189 + Synpcc7942_1333 0.14 -2.4 1,364,204 - Synpcc7942_1333 0.16 -2.0 1,364,259 - Synpcc7942_1333 0.24 -3.3 1,364,307 - Synpcc7942_1333 0.31 -2.4 1,364,312 - Synpcc7942_1333 0.32 -1.8 1,364,465 + Synpcc7942_1333 0.54 -1.9 1,364,553 + Synpcc7942_1333 0.66 -1.5 1,364,561 - Synpcc7942_1333 0.68 -3.0 1,364,630 - Synpcc7942_1333 0.77 -2.8 1,364,676 - Synpcc7942_1333 0.84 -1.4 1,365,024 + trpE Synpcc7942_1334 0.17 -1.6
Or see this region's nucleotide sequence