Experiment: BG11 with Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nirA and nirB are separated by 286 nucleotides nirB and ntcB overlap by 10 nucleotides
Synpcc7942_1240: nirA - ferredoxin-nitrite reductase, at 1,263,263 to 1,264,801
nirA
Synpcc7942_1241: nirB - nitrite reductase related protein, at 1,265,088 to 1,266,137
nirB
Synpcc7942_1242: ntcB - transcriptional regulator, LysR family, at 1,266,128 to 1,267,057
ntcB
Position (kb)
1265
1266
1267 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1264.291 kb on - strand, within nirA at 1264.965 kb on + strand at 1264.968 kb on - strand at 1264.973 kb on - strand at 1265.073 kb on - strand at 1265.083 kb on - strand at 1265.111 kb on + strand at 1265.111 kb on + strand at 1265.120 kb on - strand at 1265.157 kb on + strand at 1265.215 kb on + strand, within nirB at 1265.434 kb on - strand, within nirB at 1265.508 kb on + strand, within nirB at 1265.612 kb on - strand, within nirB at 1265.629 kb on - strand, within nirB at 1265.710 kb on - strand, within nirB at 1265.710 kb on - strand, within nirB at 1265.720 kb on - strand, within nirB at 1265.828 kb on - strand, within nirB at 1266.002 kb on + strand, within nirB at 1266.007 kb on + strand, within nirB at 1266.054 kb on + strand at 1266.056 kb on + strand at 1266.062 kb on - strand at 1266.064 kb on - strand at 1266.104 kb on + strand at 1266.104 kb on + strand at 1266.111 kb on - strand at 1266.111 kb on - strand at 1266.132 kb on + strand at 1266.142 kb on + strand at 1266.150 kb on - strand at 1266.191 kb on + strand at 1266.232 kb on + strand, within ntcB at 1266.232 kb on + strand, within ntcB at 1266.239 kb on + strand, within ntcB at 1266.247 kb on - strand, within ntcB at 1266.261 kb on - strand, within ntcB at 1266.263 kb on + strand, within ntcB at 1266.276 kb on - strand, within ntcB at 1266.354 kb on - strand, within ntcB at 1266.482 kb on + strand, within ntcB at 1266.503 kb on - strand, within ntcB at 1266.632 kb on + strand, within ntcB at 1266.650 kb on - strand, within ntcB at 1266.677 kb on - strand, within ntcB at 1266.686 kb on - strand, within ntcB at 1266.739 kb on - strand, within ntcB at 1266.768 kb on + strand, within ntcB at 1266.776 kb on - strand, within ntcB at 1266.808 kb on - strand, within ntcB at 1266.840 kb on + strand, within ntcB at 1266.862 kb on - strand, within ntcB at 1266.892 kb on - strand, within ntcB at 1266.909 kb on + strand, within ntcB at 1266.909 kb on + strand, within ntcB at 1266.912 kb on - strand, within ntcB at 1266.917 kb on - strand, within ntcB at 1266.938 kb on + strand, within ntcB at 1266.948 kb on + strand, within ntcB at 1266.948 kb on + strand, within ntcB at 1266.994 kb on + strand at 1267.051 kb on + strand at 1267.051 kb on + strand at 1267.051 kb on + strand at 1267.051 kb on + strand at 1267.051 kb on + strand at 1267.051 kb on + strand at 1267.051 kb on + strand at 1267.059 kb on - strand at 1267.059 kb on - strand at 1267.059 kb on - strand at 1267.059 kb on - strand at 1267.059 kb on - strand at 1267.059 kb on - strand at 1267.059 kb on - strand at 1267.059 kb on - strand at 1267.059 kb on - strand at 1267.070 kb on - strand at 1267.079 kb on - strand at 1267.106 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BG11 with Cisplatin 0.05 mg/ml remove 1,264,291 - nirA Synpcc7942_1240 0.67 +1.6 1,264,965 + -0.4 1,264,968 - -0.0 1,264,973 - +1.0 1,265,073 - -0.2 1,265,083 - +0.0 1,265,111 + -1.8 1,265,111 + -0.7 1,265,120 - +0.7 1,265,157 + -1.7 1,265,215 + nirB Synpcc7942_1241 0.12 -1.0 1,265,434 - nirB Synpcc7942_1241 0.33 -1.2 1,265,508 + nirB Synpcc7942_1241 0.40 -3.1 1,265,612 - nirB Synpcc7942_1241 0.50 -0.9 1,265,629 - nirB Synpcc7942_1241 0.52 -0.4 1,265,710 - nirB Synpcc7942_1241 0.59 -3.2 1,265,710 - nirB Synpcc7942_1241 0.59 -1.0 1,265,720 - nirB Synpcc7942_1241 0.60 -2.6 1,265,828 - nirB Synpcc7942_1241 0.70 -0.7 1,266,002 + nirB Synpcc7942_1241 0.87 -1.3 1,266,007 + nirB Synpcc7942_1241 0.88 -2.4 1,266,054 + +1.3 1,266,056 + -0.4 1,266,062 - -0.7 1,266,064 - -2.2 1,266,104 + +0.9 1,266,104 + -0.5 1,266,111 - -0.9 1,266,111 - -1.8 1,266,132 + -0.4 1,266,142 + -1.1 1,266,150 - -0.5 1,266,191 + -0.6 1,266,232 + ntcB Synpcc7942_1242 0.11 +1.1 1,266,232 + ntcB Synpcc7942_1242 0.11 -0.4 1,266,239 + ntcB Synpcc7942_1242 0.12 +0.2 1,266,247 - ntcB Synpcc7942_1242 0.13 +0.9 1,266,261 - ntcB Synpcc7942_1242 0.14 -2.1 1,266,263 + ntcB Synpcc7942_1242 0.15 -1.0 1,266,276 - ntcB Synpcc7942_1242 0.16 -1.5 1,266,354 - ntcB Synpcc7942_1242 0.24 -1.2 1,266,482 + ntcB Synpcc7942_1242 0.38 -2.9 1,266,503 - ntcB Synpcc7942_1242 0.40 -1.0 1,266,632 + ntcB Synpcc7942_1242 0.54 -2.0 1,266,650 - ntcB Synpcc7942_1242 0.56 +0.9 1,266,677 - ntcB Synpcc7942_1242 0.59 -1.8 1,266,686 - ntcB Synpcc7942_1242 0.60 +0.2 1,266,739 - ntcB Synpcc7942_1242 0.66 -0.4 1,266,768 + ntcB Synpcc7942_1242 0.69 +0.9 1,266,776 - ntcB Synpcc7942_1242 0.70 -3.2 1,266,808 - ntcB Synpcc7942_1242 0.73 -3.4 1,266,840 + ntcB Synpcc7942_1242 0.77 -1.4 1,266,862 - ntcB Synpcc7942_1242 0.79 -0.5 1,266,892 - ntcB Synpcc7942_1242 0.82 -0.5 1,266,909 + ntcB Synpcc7942_1242 0.84 +0.3 1,266,909 + ntcB Synpcc7942_1242 0.84 +0.1 1,266,912 - ntcB Synpcc7942_1242 0.84 -0.4 1,266,917 - ntcB Synpcc7942_1242 0.85 -1.7 1,266,938 + ntcB Synpcc7942_1242 0.87 -0.7 1,266,948 + ntcB Synpcc7942_1242 0.88 -0.7 1,266,948 + ntcB Synpcc7942_1242 0.88 -0.9 1,266,994 + +0.3 1,267,051 + -0.4 1,267,051 + -0.5 1,267,051 + -0.2 1,267,051 + -0.4 1,267,051 + +0.8 1,267,051 + +0.7 1,267,051 + -0.6 1,267,059 - +0.8 1,267,059 - +0.4 1,267,059 - +0.7 1,267,059 - +1.5 1,267,059 - +0.1 1,267,059 - -0.0 1,267,059 - -0.2 1,267,059 - -0.0 1,267,059 - +0.1 1,267,070 - -0.7 1,267,079 - +1.2 1,267,106 + -1.6
Or see this region's nucleotide sequence