Experiment: BG11 with Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt phoA and gst1 are separated by 68 nucleotides gst1 and Synpcc7942_0789 are separated by 95 nucleotides Synpcc7942_0789 and rbp3 are separated by 114 nucleotides
Synpcc7942_0787: phoA - putative purple acid phosphatase, at 783,007 to 783,885
phoA
Synpcc7942_0788: gst1 - glutathione S-transferase, at 783,954 to 784,748
gst1
Synpcc7942_0789: Synpcc7942_0789 - hypothetical protein, at 784,844 to 785,167
_0789
Synpcc7942_0790: rbp3 - RNA-binding region RNP-1, at 785,282 to 785,710
rbp3
Position (kb) 783
784
785 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 782.979 kb on + strand at 782.997 kb on + strand at 782.997 kb on + strand at 782.997 kb on + strand at 782.997 kb on + strand at 783.005 kb on - strand at 783.005 kb on - strand at 783.005 kb on - strand at 783.005 kb on - strand at 783.022 kb on + strand at 783.042 kb on + strand at 783.042 kb on + strand at 783.042 kb on + strand at 783.078 kb on - strand at 783.082 kb on - strand at 783.091 kb on + strand at 783.097 kb on - strand, within phoA at 783.105 kb on - strand, within phoA at 783.134 kb on - strand, within phoA at 783.162 kb on + strand, within phoA at 783.178 kb on + strand, within phoA at 783.227 kb on + strand, within phoA at 783.267 kb on - strand, within phoA at 783.285 kb on + strand, within phoA at 783.306 kb on + strand, within phoA at 783.306 kb on + strand, within phoA at 783.306 kb on + strand, within phoA at 783.306 kb on + strand, within phoA at 783.306 kb on + strand, within phoA at 783.306 kb on + strand, within phoA at 783.306 kb on + strand, within phoA at 783.306 kb on + strand, within phoA at 783.306 kb on + strand, within phoA at 783.306 kb on + strand, within phoA at 783.306 kb on + strand, within phoA at 783.306 kb on + strand, within phoA at 783.308 kb on + strand, within phoA at 783.314 kb on - strand, within phoA at 783.314 kb on - strand, within phoA at 783.314 kb on - strand, within phoA at 783.314 kb on - strand, within phoA at 783.314 kb on - strand, within phoA at 783.314 kb on - strand, within phoA at 783.314 kb on - strand, within phoA at 783.314 kb on - strand, within phoA at 783.315 kb on - strand, within phoA at 783.324 kb on + strand, within phoA at 783.349 kb on + strand at 783.357 kb on - strand, within phoA at 783.357 kb on - strand, within phoA at 783.388 kb on + strand, within phoA at 783.419 kb on - strand, within phoA at 783.428 kb on + strand at 783.466 kb on - strand, within phoA at 783.504 kb on + strand, within phoA at 783.523 kb on + strand at 783.523 kb on + strand, within phoA at 783.533 kb on + strand, within phoA at 783.533 kb on + strand, within phoA at 783.573 kb on - strand, within phoA at 783.607 kb on - strand, within phoA at 783.630 kb on + strand, within phoA at 783.638 kb on - strand, within phoA at 783.638 kb on - strand, within phoA at 783.638 kb on - strand, within phoA at 783.644 kb on + strand, within phoA at 783.644 kb on - strand, within phoA at 783.644 kb on - strand, within phoA at 783.652 kb on - strand, within phoA at 783.660 kb on - strand, within phoA at 783.685 kb on - strand, within phoA at 783.685 kb on - strand, within phoA at 783.709 kb on + strand, within phoA at 783.759 kb on + strand at 783.759 kb on + strand, within phoA at 783.767 kb on - strand, within phoA at 783.767 kb on - strand, within phoA at 783.777 kb on - strand at 783.784 kb on + strand, within phoA at 783.789 kb on + strand, within phoA at 783.792 kb on - strand, within phoA at 783.808 kb on + strand at 783.810 kb on + strand at 783.816 kb on - strand at 783.841 kb on + strand at 783.865 kb on - strand at 783.885 kb on + strand at 783.885 kb on + strand at 783.885 kb on + strand at 783.885 kb on + strand at 783.893 kb on - strand at 783.893 kb on - strand at 783.926 kb on - strand at 783.954 kb on - strand at 783.959 kb on - strand at 784.046 kb on + strand, within gst1 at 784.065 kb on - strand, within gst1 at 784.069 kb on + strand, within gst1 at 784.135 kb on + strand, within gst1 at 784.148 kb on + strand, within gst1 at 784.148 kb on + strand, within gst1 at 784.156 kb on - strand, within gst1 at 784.170 kb on + strand, within gst1 at 784.209 kb on + strand, within gst1 at 784.231 kb on - strand, within gst1 at 784.250 kb on + strand, within gst1 at 784.255 kb on + strand, within gst1 at 784.270 kb on + strand, within gst1 at 784.326 kb on + strand, within gst1 at 784.331 kb on + strand, within gst1 at 784.334 kb on - strand, within gst1 at 784.419 kb on + strand, within gst1 at 784.432 kb on - strand, within gst1 at 784.501 kb on - strand, within gst1 at 784.541 kb on + strand, within gst1 at 784.574 kb on - strand, within gst1 at 784.627 kb on - strand, within gst1 at 784.661 kb on - strand, within gst1 at 784.758 kb on + strand at 784.776 kb on - strand at 784.800 kb on - strand at 784.801 kb on + strand at 784.807 kb on + strand at 784.843 kb on - strand at 784.847 kb on - strand at 784.866 kb on - strand at 784.866 kb on - strand at 784.875 kb on + strand at 784.903 kb on + strand, within Synpcc7942_0789 at 784.909 kb on - strand, within Synpcc7942_0789 at 784.917 kb on - strand, within Synpcc7942_0789 at 784.957 kb on + strand, within Synpcc7942_0789 at 784.965 kb on - strand, within Synpcc7942_0789 at 784.965 kb on - strand, within Synpcc7942_0789 at 784.978 kb on - strand, within Synpcc7942_0789 at 784.991 kb on + strand, within Synpcc7942_0789 at 785.025 kb on - strand, within Synpcc7942_0789 at 785.032 kb on - strand, within Synpcc7942_0789 at 785.034 kb on + strand, within Synpcc7942_0789 at 785.034 kb on + strand, within Synpcc7942_0789 at 785.062 kb on - strand, within Synpcc7942_0789 at 785.081 kb on + strand, within Synpcc7942_0789 at 785.081 kb on + strand, within Synpcc7942_0789 at 785.144 kb on - strand at 785.148 kb on - strand at 785.152 kb on + strand at 785.152 kb on + strand at 785.158 kb on - strand at 785.165 kb on + strand at 785.166 kb on - strand at 785.264 kb on - strand at 785.264 kb on - strand at 785.264 kb on - strand at 785.291 kb on + strand at 785.378 kb on - strand, within rbp3 at 785.378 kb on - strand, within rbp3 at 785.501 kb on + strand, within rbp3 at 785.509 kb on - strand, within rbp3 at 785.563 kb on - strand, within rbp3 at 785.563 kb on - strand, within rbp3 at 785.580 kb on - strand, within rbp3 at 785.662 kb on - strand, within rbp3 at 785.665 kb on + strand, within rbp3 at 785.665 kb on + strand, within rbp3 at 785.665 kb on + strand, within rbp3 at 785.665 kb on + strand, within rbp3 at 785.673 kb on - strand at 785.673 kb on - strand at 785.673 kb on - strand at 785.673 kb on - strand at 785.720 kb on - strand at 785.738 kb on + strand at 785.746 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BG11 with Cisplatin 0.05 mg/ml remove 782,979 + -0.5 782,997 + -0.8 782,997 + +0.5 782,997 + +1.5 782,997 + -2.0 783,005 - +0.6 783,005 - +0.3 783,005 - -0.1 783,005 - -2.1 783,022 + +0.8 783,042 + -1.1 783,042 + +0.7 783,042 + -0.0 783,078 - +0.1 783,082 - +1.3 783,091 + +0.2 783,097 - phoA Synpcc7942_0787 0.10 -0.1 783,105 - phoA Synpcc7942_0787 0.11 +0.5 783,134 - phoA Synpcc7942_0787 0.14 -0.3 783,162 + phoA Synpcc7942_0787 0.18 -0.8 783,178 + phoA Synpcc7942_0787 0.19 +0.6 783,227 + phoA Synpcc7942_0787 0.25 +0.5 783,267 - phoA Synpcc7942_0787 0.30 +0.9 783,285 + phoA Synpcc7942_0787 0.32 +0.1 783,306 + phoA Synpcc7942_0787 0.34 +0.9 783,306 + phoA Synpcc7942_0787 0.34 +0.6 783,306 + phoA Synpcc7942_0787 0.34 -0.3 783,306 + phoA Synpcc7942_0787 0.34 -1.9 783,306 + phoA Synpcc7942_0787 0.34 +0.7 783,306 + phoA Synpcc7942_0787 0.34 +0.1 783,306 + phoA Synpcc7942_0787 0.34 +0.5 783,306 + phoA Synpcc7942_0787 0.34 +1.0 783,306 + phoA Synpcc7942_0787 0.34 +1.3 783,306 + phoA Synpcc7942_0787 0.34 +0.2 783,306 + phoA Synpcc7942_0787 0.34 +0.2 783,306 + phoA Synpcc7942_0787 0.34 -0.5 783,308 + phoA Synpcc7942_0787 0.34 -0.3 783,314 - phoA Synpcc7942_0787 0.35 +0.2 783,314 - phoA Synpcc7942_0787 0.35 +0.2 783,314 - phoA Synpcc7942_0787 0.35 -0.3 783,314 - phoA Synpcc7942_0787 0.35 -0.2 783,314 - phoA Synpcc7942_0787 0.35 -0.4 783,314 - phoA Synpcc7942_0787 0.35 +0.3 783,314 - phoA Synpcc7942_0787 0.35 +0.0 783,314 - phoA Synpcc7942_0787 0.35 -0.6 783,315 - phoA Synpcc7942_0787 0.35 +0.0 783,324 + phoA Synpcc7942_0787 0.36 -0.4 783,349 + +0.2 783,357 - phoA Synpcc7942_0787 0.40 -0.6 783,357 - phoA Synpcc7942_0787 0.40 +1.0 783,388 + phoA Synpcc7942_0787 0.43 +0.7 783,419 - phoA Synpcc7942_0787 0.47 +1.2 783,428 + -0.8 783,466 - phoA Synpcc7942_0787 0.52 +0.9 783,504 + phoA Synpcc7942_0787 0.57 -0.4 783,523 + +0.5 783,523 + phoA Synpcc7942_0787 0.59 +0.2 783,533 + phoA Synpcc7942_0787 0.60 +1.2 783,533 + phoA Synpcc7942_0787 0.60 +0.7 783,573 - phoA Synpcc7942_0787 0.64 +0.0 783,607 - phoA Synpcc7942_0787 0.68 -0.1 783,630 + phoA Synpcc7942_0787 0.71 -0.5 783,638 - phoA Synpcc7942_0787 0.72 -0.4 783,638 - phoA Synpcc7942_0787 0.72 -0.6 783,638 - phoA Synpcc7942_0787 0.72 -0.4 783,644 + phoA Synpcc7942_0787 0.72 +0.7 783,644 - phoA Synpcc7942_0787 0.72 +1.4 783,644 - phoA Synpcc7942_0787 0.72 +0.3 783,652 - phoA Synpcc7942_0787 0.73 +0.6 783,660 - phoA Synpcc7942_0787 0.74 -0.7 783,685 - phoA Synpcc7942_0787 0.77 +0.2 783,685 - phoA Synpcc7942_0787 0.77 +1.5 783,709 + phoA Synpcc7942_0787 0.80 +0.1 783,759 + -0.8 783,759 + phoA Synpcc7942_0787 0.86 +1.0 783,767 - phoA Synpcc7942_0787 0.86 -0.0 783,767 - phoA Synpcc7942_0787 0.86 +0.2 783,777 - +1.5 783,784 + phoA Synpcc7942_0787 0.88 +0.8 783,789 + phoA Synpcc7942_0787 0.89 +0.7 783,792 - phoA Synpcc7942_0787 0.89 +0.1 783,808 + +0.1 783,810 + -0.0 783,816 - +0.1 783,841 + +0.3 783,865 - -0.3 783,885 + +0.4 783,885 + -0.3 783,885 + -1.4 783,885 + +0.7 783,893 - -0.7 783,893 - +1.2 783,926 - +0.4 783,954 - -1.6 783,959 - -0.2 784,046 + gst1 Synpcc7942_0788 0.12 -2.5 784,065 - gst1 Synpcc7942_0788 0.14 -2.5 784,069 + gst1 Synpcc7942_0788 0.14 -2.4 784,135 + gst1 Synpcc7942_0788 0.23 -2.2 784,148 + gst1 Synpcc7942_0788 0.24 -3.9 784,148 + gst1 Synpcc7942_0788 0.24 -2.7 784,156 - gst1 Synpcc7942_0788 0.25 -1.9 784,170 + gst1 Synpcc7942_0788 0.27 -3.0 784,209 + gst1 Synpcc7942_0788 0.32 -1.8 784,231 - gst1 Synpcc7942_0788 0.35 -0.7 784,250 + gst1 Synpcc7942_0788 0.37 +0.2 784,255 + gst1 Synpcc7942_0788 0.38 -2.5 784,270 + gst1 Synpcc7942_0788 0.40 -4.3 784,326 + gst1 Synpcc7942_0788 0.47 -2.9 784,331 + gst1 Synpcc7942_0788 0.47 -3.0 784,334 - gst1 Synpcc7942_0788 0.48 -0.8 784,419 + gst1 Synpcc7942_0788 0.58 -4.7 784,432 - gst1 Synpcc7942_0788 0.60 -1.8 784,501 - gst1 Synpcc7942_0788 0.69 -3.0 784,541 + gst1 Synpcc7942_0788 0.74 -2.4 784,574 - gst1 Synpcc7942_0788 0.78 +0.9 784,627 - gst1 Synpcc7942_0788 0.85 -2.5 784,661 - gst1 Synpcc7942_0788 0.89 -2.8 784,758 + -0.7 784,776 - -1.7 784,800 - -1.0 784,801 + +0.0 784,807 + +0.8 784,843 - -0.9 784,847 - -0.1 784,866 - -0.1 784,866 - -0.2 784,875 + +1.0 784,903 + Synpcc7942_0789 0.18 +0.5 784,909 - Synpcc7942_0789 0.20 +0.2 784,917 - Synpcc7942_0789 0.23 -0.1 784,957 + Synpcc7942_0789 0.35 -0.3 784,965 - Synpcc7942_0789 0.37 +0.2 784,965 - Synpcc7942_0789 0.37 -0.4 784,978 - Synpcc7942_0789 0.41 -0.4 784,991 + Synpcc7942_0789 0.45 -0.2 785,025 - Synpcc7942_0789 0.56 +0.1 785,032 - Synpcc7942_0789 0.58 -1.2 785,034 + Synpcc7942_0789 0.59 -0.5 785,034 + Synpcc7942_0789 0.59 +0.6 785,062 - Synpcc7942_0789 0.67 -0.6 785,081 + Synpcc7942_0789 0.73 -0.3 785,081 + Synpcc7942_0789 0.73 +0.3 785,144 - -0.8 785,148 - +0.2 785,152 + +0.3 785,152 + -0.3 785,158 - +0.5 785,165 + +0.9 785,166 - +0.8 785,264 - -0.5 785,264 - -0.4 785,264 - -0.5 785,291 + -1.4 785,378 - rbp3 Synpcc7942_0790 0.22 -0.3 785,378 - rbp3 Synpcc7942_0790 0.22 -1.4 785,501 + rbp3 Synpcc7942_0790 0.51 -2.5 785,509 - rbp3 Synpcc7942_0790 0.53 -0.1 785,563 - rbp3 Synpcc7942_0790 0.66 -0.5 785,563 - rbp3 Synpcc7942_0790 0.66 -0.2 785,580 - rbp3 Synpcc7942_0790 0.69 -2.1 785,662 - rbp3 Synpcc7942_0790 0.89 +0.7 785,665 + rbp3 Synpcc7942_0790 0.89 -0.9 785,665 + rbp3 Synpcc7942_0790 0.89 -1.7 785,665 + rbp3 Synpcc7942_0790 0.89 -1.4 785,665 + rbp3 Synpcc7942_0790 0.89 -2.7 785,673 - -2.0 785,673 - -1.3 785,673 - -0.3 785,673 - -2.4 785,720 - -1.3 785,738 + -2.1 785,746 - -0.8
Or see this region's nucleotide sequence