Experiment: BG11 with Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ilvD and tRNA-Gln are separated by 150 nucleotides tRNA-Gln and Synpcc7942_0627 are separated by 18 nucleotides Synpcc7942_0627 and Synpcc7942_0628 are separated by 50 nucleotides Synpcc7942_0628 and Synpcc7942_0629 overlap by 4 nucleotides
Synpcc7942_0626: ilvD - dihydroxy-acid dehydratase, at 618,362 to 620,221
ilvD
Synpcc7942_R0037: tRNA-Gln - tRNA-Gln, at 620,372 to 620,443
tRNA-Gln
Synpcc7942_0627: Synpcc7942_0627 - hypothetical protein, at 620,462 to 621,226
_0627
Synpcc7942_0628: Synpcc7942_0628 - spermidine synthase, at 621,277 to 622,137
_0628
Synpcc7942_0629: Synpcc7942_0629 - hypothetical protein, at 622,134 to 622,340
_0629
Position (kb) 620
621
622 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 620.232 kb on + strand at 620.234 kb on + strand at 620.242 kb on - strand at 620.247 kb on + strand at 620.250 kb on - strand at 620.260 kb on - strand at 620.283 kb on - strand at 620.288 kb on - strand at 620.438 kb on + strand at 620.451 kb on - strand at 620.467 kb on - strand at 620.570 kb on + strand, within Synpcc7942_0627 at 620.574 kb on + strand, within Synpcc7942_0627 at 620.586 kb on + strand, within Synpcc7942_0627 at 620.594 kb on - strand, within Synpcc7942_0627 at 620.594 kb on - strand, within Synpcc7942_0627 at 620.629 kb on + strand, within Synpcc7942_0627 at 620.629 kb on + strand, within Synpcc7942_0627 at 620.629 kb on + strand, within Synpcc7942_0627 at 620.629 kb on + strand, within Synpcc7942_0627 at 620.629 kb on + strand, within Synpcc7942_0627 at 620.637 kb on - strand, within Synpcc7942_0627 at 620.637 kb on - strand, within Synpcc7942_0627 at 620.657 kb on + strand, within Synpcc7942_0627 at 620.686 kb on + strand, within Synpcc7942_0627 at 620.694 kb on - strand, within Synpcc7942_0627 at 620.708 kb on - strand, within Synpcc7942_0627 at 620.710 kb on - strand, within Synpcc7942_0627 at 620.731 kb on - strand, within Synpcc7942_0627 at 620.745 kb on + strand, within Synpcc7942_0627 at 620.753 kb on - strand, within Synpcc7942_0627 at 620.807 kb on - strand, within Synpcc7942_0627 at 620.811 kb on + strand, within Synpcc7942_0627 at 620.835 kb on - strand, within Synpcc7942_0627 at 620.878 kb on - strand, within Synpcc7942_0627 at 620.924 kb on - strand, within Synpcc7942_0627 at 620.953 kb on - strand, within Synpcc7942_0627 at 620.993 kb on - strand, within Synpcc7942_0627 at 621.078 kb on - strand, within Synpcc7942_0627 at 621.106 kb on + strand, within Synpcc7942_0627 at 621.106 kb on - strand, within Synpcc7942_0627 at 621.111 kb on - strand, within Synpcc7942_0627 at 621.175 kb on + strand at 621.212 kb on + strand at 621.223 kb on + strand at 621.228 kb on - strand at 621.233 kb on + strand at 621.235 kb on + strand at 621.240 kb on + strand at 621.243 kb on - strand at 621.248 kb on - strand at 621.248 kb on - strand at 621.248 kb on - strand at 621.251 kb on + strand at 621.256 kb on + strand at 621.259 kb on - strand at 621.261 kb on + strand at 621.274 kb on + strand at 621.352 kb on - strand at 621.657 kb on + strand, within Synpcc7942_0628 at 621.704 kb on + strand, within Synpcc7942_0628 at 621.712 kb on - strand, within Synpcc7942_0628 at 621.716 kb on - strand, within Synpcc7942_0628 at 621.821 kb on + strand, within Synpcc7942_0628 at 621.885 kb on + strand, within Synpcc7942_0628 at 621.930 kb on + strand, within Synpcc7942_0628 at 621.995 kb on + strand, within Synpcc7942_0628 at 622.093 kb on - strand at 622.113 kb on + strand at 622.122 kb on - strand at 622.126 kb on + strand at 622.140 kb on - strand at 622.163 kb on + strand, within Synpcc7942_0629 at 622.175 kb on - strand, within Synpcc7942_0629 at 622.200 kb on + strand, within Synpcc7942_0629 at 622.209 kb on + strand, within Synpcc7942_0629 at 622.216 kb on + strand, within Synpcc7942_0629 at 622.217 kb on - strand, within Synpcc7942_0629 at 622.217 kb on - strand, within Synpcc7942_0629 at 622.221 kb on + strand, within Synpcc7942_0629 at 622.222 kb on - strand, within Synpcc7942_0629
Per-strain Table
Position Strand Gene LocusTag Fraction BG11 with Cisplatin 0.05 mg/ml remove 620,232 + +0.2 620,234 + +0.0 620,242 - +0.4 620,247 + -0.5 620,250 - -1.3 620,260 - -0.1 620,283 - +0.1 620,288 - +1.0 620,438 + +0.0 620,451 - -0.2 620,467 - -0.0 620,570 + Synpcc7942_0627 0.14 -1.2 620,574 + Synpcc7942_0627 0.15 -1.0 620,586 + Synpcc7942_0627 0.16 -0.2 620,594 - Synpcc7942_0627 0.17 -0.7 620,594 - Synpcc7942_0627 0.17 -2.4 620,629 + Synpcc7942_0627 0.22 -2.8 620,629 + Synpcc7942_0627 0.22 -1.0 620,629 + Synpcc7942_0627 0.22 -1.3 620,629 + Synpcc7942_0627 0.22 -1.1 620,629 + Synpcc7942_0627 0.22 -1.2 620,637 - Synpcc7942_0627 0.23 -0.1 620,637 - Synpcc7942_0627 0.23 -1.3 620,657 + Synpcc7942_0627 0.25 -1.4 620,686 + Synpcc7942_0627 0.29 -1.0 620,694 - Synpcc7942_0627 0.30 -2.5 620,708 - Synpcc7942_0627 0.32 -1.1 620,710 - Synpcc7942_0627 0.32 +0.0 620,731 - Synpcc7942_0627 0.35 -1.3 620,745 + Synpcc7942_0627 0.37 -1.7 620,753 - Synpcc7942_0627 0.38 -1.3 620,807 - Synpcc7942_0627 0.45 -1.5 620,811 + Synpcc7942_0627 0.46 -1.8 620,835 - Synpcc7942_0627 0.49 +0.2 620,878 - Synpcc7942_0627 0.54 -1.7 620,924 - Synpcc7942_0627 0.60 +0.3 620,953 - Synpcc7942_0627 0.64 -1.1 620,993 - Synpcc7942_0627 0.69 -2.6 621,078 - Synpcc7942_0627 0.81 -0.1 621,106 + Synpcc7942_0627 0.84 -2.7 621,106 - Synpcc7942_0627 0.84 -4.2 621,111 - Synpcc7942_0627 0.85 -2.3 621,175 + +0.3 621,212 + -0.9 621,223 + -0.8 621,228 - +1.1 621,233 + +0.1 621,235 + -0.5 621,240 + -1.9 621,243 - -0.1 621,248 - +0.6 621,248 - +0.3 621,248 - -0.9 621,251 + +0.0 621,256 + +1.4 621,259 - +0.1 621,261 + -1.9 621,274 + -0.2 621,352 - -1.9 621,657 + Synpcc7942_0628 0.44 -2.5 621,704 + Synpcc7942_0628 0.50 -0.3 621,712 - Synpcc7942_0628 0.51 -1.1 621,716 - Synpcc7942_0628 0.51 -0.5 621,821 + Synpcc7942_0628 0.63 -1.2 621,885 + Synpcc7942_0628 0.71 -2.1 621,930 + Synpcc7942_0628 0.76 -2.2 621,995 + Synpcc7942_0628 0.83 -2.5 622,093 - -1.9 622,113 + -0.6 622,122 - -0.3 622,126 + -1.1 622,140 - -0.4 622,163 + Synpcc7942_0629 0.14 +2.0 622,175 - Synpcc7942_0629 0.20 -2.0 622,200 + Synpcc7942_0629 0.32 +0.0 622,209 + Synpcc7942_0629 0.36 -0.4 622,216 + Synpcc7942_0629 0.40 -1.0 622,217 - Synpcc7942_0629 0.40 +0.2 622,217 - Synpcc7942_0629 0.40 +0.2 622,221 + Synpcc7942_0629 0.42 -0.8 622,222 - Synpcc7942_0629 0.43 +0.2
Or see this region's nucleotide sequence