Experiment: Survival; Treatment=FW300 (pH7.4) with 5.0 mM Glucose; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_04000 and IAI47_04005 are separated by 48 nucleotides IAI47_04005 and IAI47_04010 overlap by 22 nucleotides IAI47_04010 and IAI47_04015 are separated by 2 nucleotides
IAI47_04000: IAI47_04000 - murein transglycosylase A, at 837,556 to 838,704
_04000
IAI47_04005: IAI47_04005 - tRNA cyclic N6-threonylcarbamoyladenosine(37) synthase TcdA, at 838,753 to 839,565
_04005
IAI47_04010: IAI47_04010 - cysteine desulfurase sulfur acceptor subunit CsdE, at 839,544 to 839,999
_04010
IAI47_04015: IAI47_04015 - cysteine desulfurase CsdA, at 840,002 to 841,210
_04015
Position (kb) 839
840 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 838.545 kb on + strand, within IAI47_04000 at 838.546 kb on - strand, within IAI47_04000 at 838.546 kb on - strand, within IAI47_04000 at 838.546 kb on - strand, within IAI47_04000 at 838.546 kb on - strand, within IAI47_04000 at 838.546 kb on - strand, within IAI47_04000 at 838.546 kb on - strand, within IAI47_04000 at 838.549 kb on + strand, within IAI47_04000 at 838.549 kb on + strand, within IAI47_04000 at 838.549 kb on + strand, within IAI47_04000 at 838.549 kb on + strand, within IAI47_04000 at 838.550 kb on - strand, within IAI47_04000 at 838.550 kb on - strand, within IAI47_04000 at 838.550 kb on - strand, within IAI47_04000 at 838.550 kb on - strand, within IAI47_04000 at 838.550 kb on - strand, within IAI47_04000 at 838.550 kb on - strand, within IAI47_04000 at 838.550 kb on - strand, within IAI47_04000 at 838.550 kb on - strand, within IAI47_04000 at 838.557 kb on + strand, within IAI47_04000 at 838.591 kb on + strand at 838.591 kb on + strand at 838.591 kb on + strand at 838.592 kb on - strand at 838.592 kb on - strand at 838.593 kb on + strand at 838.593 kb on + strand at 838.594 kb on - strand at 838.600 kb on + strand at 838.600 kb on + strand at 838.600 kb on + strand at 838.671 kb on + strand at 838.674 kb on + strand at 838.674 kb on + strand at 838.674 kb on + strand at 838.698 kb on + strand at 838.702 kb on + strand at 838.752 kb on + strand at 838.752 kb on + strand at 838.753 kb on - strand at 838.753 kb on - strand at 838.753 kb on - strand at 838.753 kb on - strand at 838.753 kb on - strand at 838.807 kb on + strand at 838.807 kb on + strand at 838.807 kb on + strand at 838.808 kb on - strand at 838.808 kb on - strand at 838.808 kb on - strand at 838.808 kb on - strand at 838.914 kb on + strand, within IAI47_04005 at 838.920 kb on + strand, within IAI47_04005 at 838.932 kb on + strand, within IAI47_04005 at 838.932 kb on + strand, within IAI47_04005 at 838.933 kb on - strand, within IAI47_04005 at 838.947 kb on + strand, within IAI47_04005 at 838.947 kb on + strand, within IAI47_04005 at 838.948 kb on - strand, within IAI47_04005 at 838.948 kb on - strand, within IAI47_04005 at 839.063 kb on + strand, within IAI47_04005 at 839.063 kb on + strand, within IAI47_04005 at 839.063 kb on + strand, within IAI47_04005 at 839.063 kb on + strand, within IAI47_04005 at 839.063 kb on + strand, within IAI47_04005 at 839.063 kb on + strand, within IAI47_04005 at 839.063 kb on + strand, within IAI47_04005 at 839.063 kb on + strand, within IAI47_04005 at 839.063 kb on + strand, within IAI47_04005 at 839.063 kb on + strand, within IAI47_04005 at 839.064 kb on - strand, within IAI47_04005 at 839.064 kb on - strand, within IAI47_04005 at 839.064 kb on - strand, within IAI47_04005 at 839.064 kb on - strand, within IAI47_04005 at 839.064 kb on - strand, within IAI47_04005 at 839.064 kb on - strand, within IAI47_04005 at 839.064 kb on - strand, within IAI47_04005 at 839.064 kb on - strand, within IAI47_04005 at 839.064 kb on - strand, within IAI47_04005 at 839.064 kb on - strand, within IAI47_04005 at 839.064 kb on - strand, within IAI47_04005 at 839.073 kb on + strand, within IAI47_04005 at 839.073 kb on + strand, within IAI47_04005 at 839.073 kb on + strand, within IAI47_04005 at 839.074 kb on - strand, within IAI47_04005 at 839.074 kb on - strand, within IAI47_04005 at 839.074 kb on - strand, within IAI47_04005 at 839.077 kb on - strand, within IAI47_04005 at 839.077 kb on - strand, within IAI47_04005 at 839.119 kb on + strand, within IAI47_04005 at 839.119 kb on + strand, within IAI47_04005 at 839.120 kb on - strand, within IAI47_04005 at 839.120 kb on - strand, within IAI47_04005 at 839.120 kb on - strand, within IAI47_04005 at 839.120 kb on - strand, within IAI47_04005 at 839.151 kb on + strand, within IAI47_04005 at 839.152 kb on - strand, within IAI47_04005 at 839.184 kb on + strand, within IAI47_04005 at 839.184 kb on + strand, within IAI47_04005 at 839.184 kb on + strand, within IAI47_04005 at 839.185 kb on - strand, within IAI47_04005 at 839.380 kb on + strand, within IAI47_04005 at 839.484 kb on + strand at 839.679 kb on + strand, within IAI47_04010 at 839.679 kb on + strand, within IAI47_04010 at 839.679 kb on + strand, within IAI47_04010 at 839.680 kb on - strand, within IAI47_04010 at 839.680 kb on - strand, within IAI47_04010 at 839.680 kb on - strand, within IAI47_04010 at 839.761 kb on + strand, within IAI47_04010 at 839.762 kb on - strand, within IAI47_04010 at 839.762 kb on - strand, within IAI47_04010 at 839.762 kb on - strand, within IAI47_04010 at 839.787 kb on + strand, within IAI47_04010 at 839.787 kb on + strand, within IAI47_04010 at 839.788 kb on - strand, within IAI47_04010 at 839.788 kb on - strand, within IAI47_04010 at 839.868 kb on + strand, within IAI47_04010 at 839.868 kb on + strand, within IAI47_04010 at 839.868 kb on + strand, within IAI47_04010 at 839.868 kb on + strand, within IAI47_04010 at 839.868 kb on + strand, within IAI47_04010 at 839.869 kb on - strand, within IAI47_04010 at 839.869 kb on - strand, within IAI47_04010 at 839.869 kb on - strand, within IAI47_04010 at 839.899 kb on + strand, within IAI47_04010 at 839.900 kb on - strand, within IAI47_04010 at 839.954 kb on + strand at 839.954 kb on + strand at 839.954 kb on + strand at 839.954 kb on + strand at 839.954 kb on + strand at 839.954 kb on + strand at 839.955 kb on - strand at 839.999 kb on + strand at 840.000 kb on - strand at 840.000 kb on - strand at 840.070 kb on + strand at 840.070 kb on + strand at 840.070 kb on + strand at 840.070 kb on + strand at 840.070 kb on + strand at 840.070 kb on + strand at 840.070 kb on + strand at 840.070 kb on + strand at 840.070 kb on + strand at 840.071 kb on - strand at 840.071 kb on - strand at 840.071 kb on - strand at 840.071 kb on - strand at 840.071 kb on - strand at 840.071 kb on - strand at 840.071 kb on - strand at 840.071 kb on - strand at 840.071 kb on - strand at 840.071 kb on - strand at 840.071 kb on - strand at 840.073 kb on + strand at 840.073 kb on + strand at 840.073 kb on + strand at 840.074 kb on - strand at 840.074 kb on - strand at 840.074 kb on - strand at 840.075 kb on + strand at 840.075 kb on + strand at 840.075 kb on + strand at 840.075 kb on + strand at 840.075 kb on + strand at 840.076 kb on - strand at 840.076 kb on - strand at 840.076 kb on - strand at 840.076 kb on - strand at 840.076 kb on - strand at 840.076 kb on - strand at 840.076 kb on - strand at 840.076 kb on - strand at 840.076 kb on - strand at 840.076 kb on - strand at 840.336 kb on + strand, within IAI47_04015 at 840.337 kb on - strand, within IAI47_04015 at 840.337 kb on - strand, within IAI47_04015 at 840.375 kb on - strand, within IAI47_04015 at 840.375 kb on - strand, within IAI47_04015 at 840.455 kb on + strand, within IAI47_04015 at 840.513 kb on + strand, within IAI47_04015 at 840.513 kb on + strand, within IAI47_04015 at 840.513 kb on + strand, within IAI47_04015 at 840.514 kb on - strand, within IAI47_04015 at 840.514 kb on - strand, within IAI47_04015 at 840.514 kb on - strand, within IAI47_04015 at 840.514 kb on - strand, within IAI47_04015 at 840.515 kb on + strand, within IAI47_04015 at 840.515 kb on + strand, within IAI47_04015 at 840.515 kb on + strand, within IAI47_04015 at 840.515 kb on + strand, within IAI47_04015 at 840.516 kb on - strand, within IAI47_04015 at 840.516 kb on - strand, within IAI47_04015 at 840.516 kb on - strand, within IAI47_04015 at 840.516 kb on - strand, within IAI47_04015 at 840.516 kb on - strand, within IAI47_04015 at 840.516 kb on - strand, within IAI47_04015 at 840.516 kb on - strand, within IAI47_04015 at 840.541 kb on - strand, within IAI47_04015 at 840.548 kb on - strand, within IAI47_04015 at 840.548 kb on - strand, within IAI47_04015 at 840.548 kb on - strand, within IAI47_04015 at 840.564 kb on + strand, within IAI47_04015 at 840.565 kb on - strand, within IAI47_04015 at 840.587 kb on + strand, within IAI47_04015 at 840.588 kb on - strand, within IAI47_04015 at 840.588 kb on - strand, within IAI47_04015 at 840.588 kb on - strand, within IAI47_04015 at 840.713 kb on + strand, within IAI47_04015 at 840.713 kb on + strand, within IAI47_04015 at 840.713 kb on + strand, within IAI47_04015 at 840.713 kb on + strand, within IAI47_04015 at 840.714 kb on - strand, within IAI47_04015 at 840.714 kb on - strand, within IAI47_04015 at 840.714 kb on - strand, within IAI47_04015 at 840.714 kb on - strand, within IAI47_04015 at 840.714 kb on - strand, within IAI47_04015 at 840.752 kb on + strand, within IAI47_04015 at 840.752 kb on + strand, within IAI47_04015 at 840.786 kb on - strand, within IAI47_04015 at 840.797 kb on + strand, within IAI47_04015 at 840.798 kb on - strand, within IAI47_04015 at 840.929 kb on + strand, within IAI47_04015 at 840.930 kb on - strand, within IAI47_04015 at 840.958 kb on + strand, within IAI47_04015 at 840.958 kb on + strand, within IAI47_04015
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 5.0 mM Glucose; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 838,545 + IAI47_04000 0.86 +0.6 838,546 - IAI47_04000 0.86 +2.6 838,546 - IAI47_04000 0.86 -0.3 838,546 - IAI47_04000 0.86 +0.5 838,546 - IAI47_04000 0.86 -0.1 838,546 - IAI47_04000 0.86 +0.2 838,546 - IAI47_04000 0.86 -0.7 838,549 + IAI47_04000 0.86 +0.2 838,549 + IAI47_04000 0.86 +1.9 838,549 + IAI47_04000 0.86 -0.5 838,549 + IAI47_04000 0.86 +2.8 838,550 - IAI47_04000 0.87 +0.4 838,550 - IAI47_04000 0.87 -0.4 838,550 - IAI47_04000 0.87 +0.1 838,550 - IAI47_04000 0.87 -1.3 838,550 - IAI47_04000 0.87 +0.2 838,550 - IAI47_04000 0.87 -2.0 838,550 - IAI47_04000 0.87 -0.4 838,550 - IAI47_04000 0.87 +1.0 838,557 + IAI47_04000 0.87 +1.5 838,591 + +0.5 838,591 + -0.4 838,591 + +0.5 838,592 - +0.4 838,592 - -0.4 838,593 + +0.6 838,593 + -1.3 838,594 - +1.2 838,600 + +1.1 838,600 + +1.8 838,600 + -2.4 838,671 + -0.2 838,674 + +2.6 838,674 + +1.3 838,674 + +0.6 838,698 + +0.2 838,702 + +3.5 838,752 + -0.7 838,752 + +0.5 838,753 - -1.2 838,753 - -0.5 838,753 - +1.4 838,753 - +0.2 838,753 - +0.1 838,807 + -0.2 838,807 + +1.9 838,807 + +0.4 838,808 - +0.6 838,808 - -0.5 838,808 - -0.2 838,808 - -0.7 838,914 + IAI47_04005 0.20 +2.2 838,920 + IAI47_04005 0.21 -0.3 838,932 + IAI47_04005 0.22 -1.7 838,932 + IAI47_04005 0.22 -1.8 838,933 - IAI47_04005 0.22 -1.2 838,947 + IAI47_04005 0.24 -0.4 838,947 + IAI47_04005 0.24 +2.7 838,948 - IAI47_04005 0.24 +0.4 838,948 - IAI47_04005 0.24 -0.7 839,063 + IAI47_04005 0.38 +0.7 839,063 + IAI47_04005 0.38 -0.7 839,063 + IAI47_04005 0.38 +1.3 839,063 + IAI47_04005 0.38 +2.7 839,063 + IAI47_04005 0.38 -0.8 839,063 + IAI47_04005 0.38 +0.7 839,063 + IAI47_04005 0.38 +0.3 839,063 + IAI47_04005 0.38 +0.2 839,063 + IAI47_04005 0.38 -0.2 839,063 + IAI47_04005 0.38 +0.5 839,064 - IAI47_04005 0.38 +0.6 839,064 - IAI47_04005 0.38 -1.4 839,064 - IAI47_04005 0.38 +0.2 839,064 - IAI47_04005 0.38 +0.1 839,064 - IAI47_04005 0.38 -0.8 839,064 - IAI47_04005 0.38 +0.2 839,064 - IAI47_04005 0.38 -0.6 839,064 - IAI47_04005 0.38 -0.5 839,064 - IAI47_04005 0.38 +1.0 839,064 - IAI47_04005 0.38 -0.2 839,064 - IAI47_04005 0.38 +0.7 839,073 + IAI47_04005 0.39 +1.0 839,073 + IAI47_04005 0.39 +0.3 839,073 + IAI47_04005 0.39 +1.2 839,074 - IAI47_04005 0.39 -0.0 839,074 - IAI47_04005 0.39 +0.2 839,074 - IAI47_04005 0.39 +0.4 839,077 - IAI47_04005 0.40 +0.9 839,077 - IAI47_04005 0.40 -1.7 839,119 + IAI47_04005 0.45 +0.5 839,119 + IAI47_04005 0.45 +0.9 839,120 - IAI47_04005 0.45 -1.9 839,120 - IAI47_04005 0.45 +0.4 839,120 - IAI47_04005 0.45 -0.7 839,120 - IAI47_04005 0.45 -1.1 839,151 + IAI47_04005 0.49 +2.6 839,152 - IAI47_04005 0.49 +0.3 839,184 + IAI47_04005 0.53 +0.9 839,184 + IAI47_04005 0.53 +0.6 839,184 + IAI47_04005 0.53 -3.0 839,185 - IAI47_04005 0.53 -0.7 839,380 + IAI47_04005 0.77 +0.7 839,484 + +0.4 839,679 + IAI47_04010 0.30 -0.7 839,679 + IAI47_04010 0.30 -0.6 839,679 + IAI47_04010 0.30 +0.1 839,680 - IAI47_04010 0.30 +0.1 839,680 - IAI47_04010 0.30 +0.7 839,680 - IAI47_04010 0.30 +0.6 839,761 + IAI47_04010 0.48 -0.2 839,762 - IAI47_04010 0.48 +1.8 839,762 - IAI47_04010 0.48 -1.1 839,762 - IAI47_04010 0.48 -1.3 839,787 + IAI47_04010 0.53 -0.2 839,787 + IAI47_04010 0.53 -0.2 839,788 - IAI47_04010 0.54 -0.7 839,788 - IAI47_04010 0.54 +0.6 839,868 + IAI47_04010 0.71 -2.0 839,868 + IAI47_04010 0.71 -0.5 839,868 + IAI47_04010 0.71 -0.2 839,868 + IAI47_04010 0.71 -0.4 839,868 + IAI47_04010 0.71 +0.0 839,869 - IAI47_04010 0.71 +0.9 839,869 - IAI47_04010 0.71 -1.0 839,869 - IAI47_04010 0.71 -0.3 839,899 + IAI47_04010 0.78 -1.4 839,900 - IAI47_04010 0.78 +2.8 839,954 + -0.7 839,954 + -0.8 839,954 + +0.6 839,954 + +0.0 839,954 + -0.6 839,954 + -1.3 839,955 - -0.8 839,999 + +0.5 840,000 - -0.4 840,000 - +0.2 840,070 + +0.5 840,070 + -0.5 840,070 + +0.4 840,070 + +0.7 840,070 + -0.9 840,070 + +1.4 840,070 + -0.8 840,070 + +0.0 840,070 + +0.9 840,071 - -0.0 840,071 - -0.8 840,071 - +0.9 840,071 - -1.8 840,071 - +0.3 840,071 - +2.1 840,071 - +1.7 840,071 - +0.9 840,071 - -0.3 840,071 - -0.8 840,071 - +1.1 840,073 + -0.2 840,073 + -0.3 840,073 + -0.4 840,074 - +1.1 840,074 - +1.4 840,074 - +0.2 840,075 + -2.8 840,075 + +0.9 840,075 + +0.1 840,075 + +0.6 840,075 + -0.4 840,076 - +0.6 840,076 - +0.8 840,076 - +1.6 840,076 - -0.3 840,076 - +0.6 840,076 - -0.6 840,076 - +1.2 840,076 - +1.4 840,076 - +1.3 840,076 - +0.4 840,336 + IAI47_04015 0.28 -2.4 840,337 - IAI47_04015 0.28 +4.9 840,337 - IAI47_04015 0.28 +0.7 840,375 - IAI47_04015 0.31 -2.3 840,375 - IAI47_04015 0.31 -0.2 840,455 + IAI47_04015 0.37 -0.3 840,513 + IAI47_04015 0.42 -1.1 840,513 + IAI47_04015 0.42 -0.7 840,513 + IAI47_04015 0.42 -0.2 840,514 - IAI47_04015 0.42 +3.3 840,514 - IAI47_04015 0.42 +0.2 840,514 - IAI47_04015 0.42 +1.8 840,514 - IAI47_04015 0.42 -1.8 840,515 + IAI47_04015 0.42 -1.6 840,515 + IAI47_04015 0.42 -1.5 840,515 + IAI47_04015 0.42 +0.4 840,515 + IAI47_04015 0.42 +0.5 840,516 - IAI47_04015 0.43 +1.9 840,516 - IAI47_04015 0.43 +0.5 840,516 - IAI47_04015 0.43 +1.2 840,516 - IAI47_04015 0.43 +0.4 840,516 - IAI47_04015 0.43 -1.4 840,516 - IAI47_04015 0.43 -0.6 840,516 - IAI47_04015 0.43 +0.5 840,541 - IAI47_04015 0.45 +0.0 840,548 - IAI47_04015 0.45 +1.1 840,548 - IAI47_04015 0.45 +2.8 840,548 - IAI47_04015 0.45 -0.2 840,564 + IAI47_04015 0.46 +0.6 840,565 - IAI47_04015 0.47 -0.7 840,587 + IAI47_04015 0.48 +1.4 840,588 - IAI47_04015 0.48 -0.1 840,588 - IAI47_04015 0.48 +0.1 840,588 - IAI47_04015 0.48 +0.6 840,713 + IAI47_04015 0.59 +2.2 840,713 + IAI47_04015 0.59 -0.7 840,713 + IAI47_04015 0.59 +2.6 840,713 + IAI47_04015 0.59 -0.1 840,714 - IAI47_04015 0.59 +2.0 840,714 - IAI47_04015 0.59 -0.7 840,714 - IAI47_04015 0.59 -0.3 840,714 - IAI47_04015 0.59 +2.9 840,714 - IAI47_04015 0.59 +0.6 840,752 + IAI47_04015 0.62 +0.3 840,752 + IAI47_04015 0.62 -0.7 840,786 - IAI47_04015 0.65 +0.5 840,797 + IAI47_04015 0.66 -0.6 840,798 - IAI47_04015 0.66 +0.4 840,929 + IAI47_04015 0.77 +1.2 840,930 - IAI47_04015 0.77 +2.7 840,958 + IAI47_04015 0.79 +0.2 840,958 + IAI47_04015 0.79 +0.4
Or see this region's nucleotide sequence