Experiment: PYE_noCalcium 30C survival rep2 28 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00250 and CCNA_00251 are separated by 48 nucleotides CCNA_00251 and CCNA_00252 are separated by 111 nucleotides CCNA_00252 and CCNA_00253 are separated by 253 nucleotides
CCNA_00250: CCNA_00250 - poly(3-hydroxyalkanoate) depolymerase, at 261,642 to 262,904
_00250
CCNA_00251: CCNA_00251 - zinc metalloprotease, at 262,953 to 263,672
_00251
CCNA_00252: CCNA_00252 - multimodular transpeptidase-transglycosylase PbpX, at 263,784 to 265,745
_00252
CCNA_00253: CCNA_00253 - aspartate-semialdehyde dehydrogenase, at 265,999 to 267,027
_00253
Position (kb) 263
264
265
266 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 262.791 kb on - strand at 262.814 kb on + strand at 262.849 kb on - strand at 262.906 kb on - strand at 262.923 kb on - strand at 262.950 kb on - strand at 262.950 kb on - strand at 262.968 kb on + strand at 262.968 kb on + strand at 263.016 kb on + strand at 263.021 kb on + strand at 263.029 kb on - strand, within CCNA_00251 at 263.069 kb on + strand, within CCNA_00251 at 263.074 kb on + strand, within CCNA_00251 at 263.095 kb on + strand, within CCNA_00251 at 263.101 kb on + strand, within CCNA_00251 at 263.101 kb on - strand, within CCNA_00251 at 263.146 kb on - strand, within CCNA_00251 at 263.204 kb on - strand, within CCNA_00251 at 263.216 kb on - strand, within CCNA_00251 at 263.294 kb on - strand, within CCNA_00251 at 263.316 kb on - strand, within CCNA_00251 at 263.333 kb on + strand, within CCNA_00251 at 263.340 kb on + strand, within CCNA_00251 at 263.340 kb on + strand, within CCNA_00251 at 263.348 kb on - strand, within CCNA_00251 at 263.373 kb on - strand, within CCNA_00251 at 263.382 kb on + strand, within CCNA_00251 at 263.382 kb on + strand, within CCNA_00251 at 263.382 kb on + strand, within CCNA_00251 at 263.383 kb on + strand, within CCNA_00251 at 263.390 kb on - strand, within CCNA_00251 at 263.446 kb on + strand, within CCNA_00251 at 263.455 kb on - strand, within CCNA_00251 at 263.530 kb on + strand, within CCNA_00251 at 263.547 kb on + strand, within CCNA_00251 at 263.552 kb on + strand, within CCNA_00251 at 263.555 kb on - strand, within CCNA_00251 at 263.576 kb on + strand, within CCNA_00251 at 263.578 kb on + strand, within CCNA_00251 at 263.665 kb on + strand at 263.698 kb on + strand at 263.711 kb on - strand at 263.718 kb on - strand at 263.720 kb on + strand at 263.728 kb on - strand at 263.728 kb on - strand at 263.732 kb on + strand at 263.736 kb on + strand at 263.739 kb on - strand at 263.759 kb on + strand at 263.768 kb on - strand at 263.785 kb on - strand at 263.790 kb on - strand at 263.806 kb on - strand at 263.834 kb on - strand at 263.889 kb on - strand at 263.904 kb on - strand at 263.935 kb on + strand at 263.967 kb on - strand at 263.971 kb on + strand at 263.971 kb on + strand at 263.971 kb on + strand at 263.971 kb on + strand at 264.025 kb on - strand, within CCNA_00252 at 264.027 kb on + strand, within CCNA_00252 at 264.030 kb on - strand, within CCNA_00252 at 264.035 kb on - strand, within CCNA_00252 at 264.035 kb on - strand, within CCNA_00252 at 264.036 kb on + strand, within CCNA_00252 at 264.036 kb on + strand, within CCNA_00252 at 264.085 kb on + strand, within CCNA_00252 at 264.093 kb on - strand, within CCNA_00252 at 264.093 kb on - strand, within CCNA_00252 at 264.200 kb on + strand, within CCNA_00252 at 264.213 kb on + strand, within CCNA_00252 at 264.241 kb on + strand, within CCNA_00252 at 264.253 kb on + strand, within CCNA_00252 at 264.261 kb on - strand, within CCNA_00252 at 264.261 kb on - strand, within CCNA_00252 at 264.265 kb on - strand, within CCNA_00252 at 264.290 kb on - strand, within CCNA_00252 at 264.300 kb on - strand, within CCNA_00252 at 264.300 kb on - strand, within CCNA_00252 at 264.329 kb on + strand, within CCNA_00252 at 264.329 kb on - strand, within CCNA_00252 at 264.331 kb on + strand, within CCNA_00252 at 264.336 kb on + strand, within CCNA_00252 at 264.372 kb on + strand, within CCNA_00252 at 264.397 kb on - strand, within CCNA_00252 at 264.421 kb on - strand, within CCNA_00252 at 264.436 kb on - strand, within CCNA_00252 at 264.495 kb on + strand, within CCNA_00252 at 264.495 kb on + strand, within CCNA_00252 at 264.512 kb on + strand, within CCNA_00252 at 264.520 kb on + strand, within CCNA_00252 at 264.520 kb on - strand, within CCNA_00252 at 264.582 kb on - strand, within CCNA_00252 at 264.800 kb on + strand, within CCNA_00252 at 264.800 kb on + strand, within CCNA_00252 at 264.844 kb on - strand, within CCNA_00252 at 264.891 kb on + strand, within CCNA_00252 at 264.891 kb on + strand, within CCNA_00252 at 264.913 kb on + strand, within CCNA_00252 at 264.936 kb on + strand, within CCNA_00252 at 264.946 kb on + strand, within CCNA_00252 at 264.954 kb on - strand, within CCNA_00252 at 264.994 kb on - strand, within CCNA_00252 at 264.994 kb on - strand, within CCNA_00252 at 265.025 kb on + strand, within CCNA_00252 at 265.036 kb on - strand, within CCNA_00252 at 265.203 kb on - strand, within CCNA_00252 at 265.235 kb on - strand, within CCNA_00252 at 265.250 kb on - strand, within CCNA_00252 at 265.250 kb on - strand, within CCNA_00252 at 265.293 kb on + strand, within CCNA_00252 at 265.293 kb on + strand, within CCNA_00252 at 265.293 kb on + strand, within CCNA_00252 at 265.324 kb on - strand, within CCNA_00252 at 265.339 kb on - strand, within CCNA_00252 at 265.339 kb on - strand, within CCNA_00252 at 265.340 kb on + strand, within CCNA_00252 at 265.361 kb on - strand, within CCNA_00252 at 265.385 kb on - strand, within CCNA_00252 at 265.418 kb on - strand, within CCNA_00252 at 265.535 kb on - strand, within CCNA_00252 at 265.552 kb on - strand at 265.570 kb on + strand at 265.576 kb on + strand at 265.578 kb on - strand at 265.579 kb on + strand at 265.586 kb on + strand at 265.594 kb on - strand at 265.619 kb on - strand at 265.636 kb on + strand at 265.680 kb on - strand at 265.684 kb on + strand at 265.746 kb on - strand at 265.755 kb on + strand at 265.771 kb on + strand at 265.771 kb on + strand at 265.771 kb on + strand at 265.786 kb on + strand at 265.792 kb on - strand at 265.794 kb on - strand at 265.800 kb on - strand at 265.802 kb on - strand at 265.803 kb on + strand at 265.852 kb on - strand at 265.858 kb on + strand at 265.890 kb on + strand at 265.892 kb on + strand at 265.904 kb on - strand at 265.911 kb on - strand at 265.941 kb on + strand at 265.982 kb on + strand at 265.982 kb on + strand at 265.982 kb on + strand at 265.982 kb on + strand at 265.984 kb on + strand at 265.984 kb on + strand at 265.984 kb on + strand at 265.984 kb on + strand at 265.984 kb on + strand at 265.990 kb on - strand at 265.990 kb on - strand at 265.990 kb on - strand at 265.990 kb on - strand at 265.990 kb on - strand at 265.990 kb on - strand at 265.990 kb on - strand at 265.992 kb on - strand at 266.001 kb on + strand at 266.001 kb on + strand at 266.071 kb on + strand at 266.091 kb on + strand at 266.107 kb on - strand, within CCNA_00253 at 266.108 kb on + strand, within CCNA_00253 at 266.159 kb on + strand, within CCNA_00253 at 266.173 kb on - strand, within CCNA_00253 at 266.173 kb on - strand, within CCNA_00253 at 266.190 kb on + strand, within CCNA_00253 at 266.192 kb on - strand, within CCNA_00253 at 266.208 kb on - strand, within CCNA_00253 at 266.234 kb on - strand, within CCNA_00253 at 266.240 kb on - strand, within CCNA_00253 at 266.240 kb on - strand, within CCNA_00253 at 266.240 kb on - strand, within CCNA_00253 at 266.240 kb on - strand, within CCNA_00253 at 266.240 kb on - strand, within CCNA_00253 at 266.240 kb on - strand, within CCNA_00253 at 266.279 kb on + strand, within CCNA_00253 at 266.299 kb on + strand, within CCNA_00253 at 266.312 kb on + strand, within CCNA_00253 at 266.341 kb on + strand, within CCNA_00253 at 266.364 kb on + strand, within CCNA_00253 at 266.396 kb on + strand, within CCNA_00253 at 266.427 kb on - strand, within CCNA_00253 at 266.430 kb on + strand, within CCNA_00253 at 266.430 kb on + strand, within CCNA_00253 at 266.451 kb on + strand, within CCNA_00253 at 266.458 kb on + strand, within CCNA_00253 at 266.482 kb on + strand, within CCNA_00253 at 266.482 kb on + strand, within CCNA_00253 at 266.490 kb on + strand, within CCNA_00253 at 266.505 kb on - strand, within CCNA_00253 at 266.514 kb on + strand, within CCNA_00253 at 266.514 kb on + strand, within CCNA_00253 at 266.514 kb on + strand, within CCNA_00253 at 266.522 kb on - strand, within CCNA_00253 at 266.525 kb on + strand, within CCNA_00253 at 266.573 kb on + strand, within CCNA_00253 at 266.608 kb on + strand, within CCNA_00253 at 266.627 kb on - strand, within CCNA_00253 at 266.650 kb on + strand, within CCNA_00253 at 266.706 kb on + strand, within CCNA_00253 at 266.714 kb on - strand, within CCNA_00253 at 266.728 kb on - strand, within CCNA_00253 at 266.730 kb on + strand, within CCNA_00253
Per-strain Table
Position Strand Gene LocusTag Fraction PYE_noCalcium 30C survival rep2 28 days remove 262,791 - -0.9 262,814 + -2.7 262,849 - -1.2 262,906 - -1.0 262,923 - -0.8 262,950 - -0.9 262,950 - -0.9 262,968 + -0.5 262,968 + +1.8 263,016 + +2.1 263,021 + +0.1 263,029 - CCNA_00251 0.11 +1.9 263,069 + CCNA_00251 0.16 -1.8 263,074 + CCNA_00251 0.17 +0.4 263,095 + CCNA_00251 0.20 +0.8 263,101 + CCNA_00251 0.21 +0.8 263,101 - CCNA_00251 0.21 -1.2 263,146 - CCNA_00251 0.27 -0.6 263,204 - CCNA_00251 0.35 -2.4 263,216 - CCNA_00251 0.37 +0.6 263,294 - CCNA_00251 0.47 -1.2 263,316 - CCNA_00251 0.50 -2.7 263,333 + CCNA_00251 0.53 +0.3 263,340 + CCNA_00251 0.54 +0.5 263,340 + CCNA_00251 0.54 -1.1 263,348 - CCNA_00251 0.55 -2.5 263,373 - CCNA_00251 0.58 -0.4 263,382 + CCNA_00251 0.60 -1.6 263,382 + CCNA_00251 0.60 -1.6 263,382 + CCNA_00251 0.60 -0.1 263,383 + CCNA_00251 0.60 -0.7 263,390 - CCNA_00251 0.61 -2.0 263,446 + CCNA_00251 0.68 -0.8 263,455 - CCNA_00251 0.70 -0.6 263,530 + CCNA_00251 0.80 -3.4 263,547 + CCNA_00251 0.82 +0.5 263,552 + CCNA_00251 0.83 -2.6 263,555 - CCNA_00251 0.84 +0.1 263,576 + CCNA_00251 0.87 -2.5 263,578 + CCNA_00251 0.87 +0.5 263,665 + +0.1 263,698 + +1.4 263,711 - -1.2 263,718 - -1.9 263,720 + -1.7 263,728 - +1.5 263,728 - +0.0 263,732 + +1.8 263,736 + -2.0 263,739 - -0.5 263,759 + +1.1 263,768 - -0.8 263,785 - +1.3 263,790 - -1.1 263,806 - -0.5 263,834 - -1.1 263,889 - -1.2 263,904 - -0.3 263,935 + -0.3 263,967 - -1.5 263,971 + +0.5 263,971 + +0.9 263,971 + -0.9 263,971 + +1.5 264,025 - CCNA_00252 0.12 -0.1 264,027 + CCNA_00252 0.12 -0.3 264,030 - CCNA_00252 0.13 +2.5 264,035 - CCNA_00252 0.13 -3.1 264,035 - CCNA_00252 0.13 -2.5 264,036 + CCNA_00252 0.13 -1.9 264,036 + CCNA_00252 0.13 +0.2 264,085 + CCNA_00252 0.15 -0.9 264,093 - CCNA_00252 0.16 +0.5 264,093 - CCNA_00252 0.16 -0.8 264,200 + CCNA_00252 0.21 +0.2 264,213 + CCNA_00252 0.22 +0.2 264,241 + CCNA_00252 0.23 -2.1 264,253 + CCNA_00252 0.24 +0.5 264,261 - CCNA_00252 0.24 -1.3 264,261 - CCNA_00252 0.24 -0.6 264,265 - CCNA_00252 0.25 -1.3 264,290 - CCNA_00252 0.26 +0.5 264,300 - CCNA_00252 0.26 -1.1 264,300 - CCNA_00252 0.26 -2.7 264,329 + CCNA_00252 0.28 -1.5 264,329 - CCNA_00252 0.28 -0.1 264,331 + CCNA_00252 0.28 -1.5 264,336 + CCNA_00252 0.28 +0.2 264,372 + CCNA_00252 0.30 +0.1 264,397 - CCNA_00252 0.31 -0.8 264,421 - CCNA_00252 0.32 +0.9 264,436 - CCNA_00252 0.33 +0.5 264,495 + CCNA_00252 0.36 -1.7 264,495 + CCNA_00252 0.36 -2.2 264,512 + CCNA_00252 0.37 -1.4 264,520 + CCNA_00252 0.38 +0.2 264,520 - CCNA_00252 0.38 -1.1 264,582 - CCNA_00252 0.41 -1.6 264,800 + CCNA_00252 0.52 +0.5 264,800 + CCNA_00252 0.52 -2.2 264,844 - CCNA_00252 0.54 -0.5 264,891 + CCNA_00252 0.56 +2.5 264,891 + CCNA_00252 0.56 +2.5 264,913 + CCNA_00252 0.58 +1.5 264,936 + CCNA_00252 0.59 +2.5 264,946 + CCNA_00252 0.59 +0.5 264,954 - CCNA_00252 0.60 -0.1 264,994 - CCNA_00252 0.62 -2.0 264,994 - CCNA_00252 0.62 +3.5 265,025 + CCNA_00252 0.63 +0.9 265,036 - CCNA_00252 0.64 -0.3 265,203 - CCNA_00252 0.72 -0.3 265,235 - CCNA_00252 0.74 -2.1 265,250 - CCNA_00252 0.75 -0.9 265,250 - CCNA_00252 0.75 -0.2 265,293 + CCNA_00252 0.77 +2.5 265,293 + CCNA_00252 0.77 -0.3 265,293 + CCNA_00252 0.77 +0.9 265,324 - CCNA_00252 0.78 -2.4 265,339 - CCNA_00252 0.79 -1.9 265,339 - CCNA_00252 0.79 +0.2 265,340 + CCNA_00252 0.79 -0.3 265,361 - CCNA_00252 0.80 -1.4 265,385 - CCNA_00252 0.82 -2.0 265,418 - CCNA_00252 0.83 -0.8 265,535 - CCNA_00252 0.89 -0.6 265,552 - +2.5 265,570 + -1.4 265,576 + -1.9 265,578 - -0.6 265,579 + -3.7 265,586 + +2.5 265,594 - -0.3 265,619 - -0.3 265,636 + -1.2 265,680 - -0.8 265,684 + -2.7 265,746 - +3.4 265,755 + -1.6 265,771 + +0.4 265,771 + -0.1 265,771 + +4.5 265,786 + -0.8 265,792 - +0.6 265,794 - +0.4 265,800 - -3.6 265,802 - -2.9 265,803 + +0.9 265,852 - -0.5 265,858 + -0.3 265,890 + -0.3 265,892 + +0.2 265,904 - +1.3 265,911 - -0.9 265,941 + +0.4 265,982 + -2.1 265,982 + -0.3 265,982 + +1.1 265,982 + -0.5 265,984 + -0.4 265,984 + +0.1 265,984 + -1.0 265,984 + -0.9 265,984 + -2.0 265,990 - +0.5 265,990 - -0.7 265,990 - -0.5 265,990 - -0.6 265,990 - -2.4 265,990 - -2.8 265,990 - -0.1 265,992 - +1.5 266,001 + -2.8 266,001 + +1.1 266,071 + +1.2 266,091 + -0.7 266,107 - CCNA_00253 0.10 +0.9 266,108 + CCNA_00253 0.11 -2.3 266,159 + CCNA_00253 0.16 -2.3 266,173 - CCNA_00253 0.17 +1.5 266,173 - CCNA_00253 0.17 +0.9 266,190 + CCNA_00253 0.19 -2.4 266,192 - CCNA_00253 0.19 -2.7 266,208 - CCNA_00253 0.20 -0.3 266,234 - CCNA_00253 0.23 -2.0 266,240 - CCNA_00253 0.23 -2.2 266,240 - CCNA_00253 0.23 -1.2 266,240 - CCNA_00253 0.23 -0.9 266,240 - CCNA_00253 0.23 -2.7 266,240 - CCNA_00253 0.23 -3.1 266,240 - CCNA_00253 0.23 -0.1 266,279 + CCNA_00253 0.27 +0.2 266,299 + CCNA_00253 0.29 -1.8 266,312 + CCNA_00253 0.30 -2.5 266,341 + CCNA_00253 0.33 +0.1 266,364 + CCNA_00253 0.35 +0.4 266,396 + CCNA_00253 0.39 -2.4 266,427 - CCNA_00253 0.42 -2.6 266,430 + CCNA_00253 0.42 -0.7 266,430 + CCNA_00253 0.42 -1.4 266,451 + CCNA_00253 0.44 -2.0 266,458 + CCNA_00253 0.45 -0.5 266,482 + CCNA_00253 0.47 -2.5 266,482 + CCNA_00253 0.47 -0.3 266,490 + CCNA_00253 0.48 +0.1 266,505 - CCNA_00253 0.49 +2.5 266,514 + CCNA_00253 0.50 +0.5 266,514 + CCNA_00253 0.50 +0.9 266,514 + CCNA_00253 0.50 -1.6 266,522 - CCNA_00253 0.51 -1.6 266,525 + CCNA_00253 0.51 -2.8 266,573 + CCNA_00253 0.56 -0.6 266,608 + CCNA_00253 0.59 -2.1 266,627 - CCNA_00253 0.61 +0.2 266,650 + CCNA_00253 0.63 -1.3 266,706 + CCNA_00253 0.69 +0.0 266,714 - CCNA_00253 0.69 -0.3 266,728 - CCNA_00253 0.71 +0.5 266,730 + CCNA_00253 0.71 +0.6
Or see this region's nucleotide sequence