Experiment: Survival; Treatment=FW300 (pH7.4) with 5.0 mM Glucose; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_01515 and IAI47_01520 are separated by 95 nucleotides IAI47_01520 and IAI47_01525 are separated by 145 nucleotides IAI47_01525 and IAI47_01530 are separated by 26 nucleotides IAI47_01530 and IAI47_01535 overlap by 11 nucleotides
IAI47_01515: IAI47_01515 - zinc/cadmium/mercury/lead-transporting ATPase, at 330,031 to 332,316
_01515
IAI47_01520: IAI47_01520 - lysoplasmalogenase, at 332,412 to 333,041
_01520
IAI47_01525: IAI47_01525 - DUF2500 domain-containing protein, at 333,187 to 333,543
_01525
IAI47_01530: IAI47_01530 - DUF1145 family protein, at 333,570 to 333,842
_01530
IAI47_01535: IAI47_01535 - 16S rRNA (guanine(966)-N(2))-methyltransferase, at 333,832 to 334,431
_01535
Position (kb) 332
333
334 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 331.414 kb on + strand, within IAI47_01515 at 331.414 kb on + strand, within IAI47_01515 at 331.415 kb on - strand, within IAI47_01515 at 331.521 kb on + strand, within IAI47_01515 at 331.522 kb on - strand, within IAI47_01515 at 331.583 kb on + strand, within IAI47_01515 at 331.583 kb on + strand, within IAI47_01515 at 331.584 kb on - strand, within IAI47_01515 at 331.584 kb on - strand, within IAI47_01515 at 331.584 kb on - strand, within IAI47_01515 at 331.584 kb on - strand, within IAI47_01515 at 331.584 kb on - strand, within IAI47_01515 at 331.650 kb on + strand, within IAI47_01515 at 331.651 kb on - strand, within IAI47_01515 at 331.681 kb on + strand, within IAI47_01515 at 331.681 kb on + strand, within IAI47_01515 at 331.681 kb on + strand, within IAI47_01515 at 331.681 kb on + strand, within IAI47_01515 at 331.682 kb on - strand, within IAI47_01515 at 331.682 kb on - strand, within IAI47_01515 at 331.695 kb on + strand, within IAI47_01515 at 331.695 kb on + strand, within IAI47_01515 at 331.696 kb on - strand, within IAI47_01515 at 331.696 kb on - strand, within IAI47_01515 at 331.826 kb on - strand, within IAI47_01515 at 332.026 kb on + strand, within IAI47_01515 at 332.026 kb on + strand, within IAI47_01515 at 332.027 kb on - strand, within IAI47_01515 at 332.094 kb on + strand at 332.094 kb on + strand at 332.095 kb on - strand at 332.095 kb on - strand at 332.095 kb on - strand at 332.095 kb on - strand at 332.160 kb on + strand at 332.160 kb on + strand at 332.161 kb on - strand at 332.161 kb on - strand at 332.184 kb on + strand at 332.185 kb on - strand at 332.185 kb on - strand at 332.185 kb on - strand at 332.195 kb on - strand at 332.202 kb on + strand at 332.202 kb on + strand at 332.203 kb on - strand at 332.203 kb on - strand at 332.214 kb on + strand at 332.214 kb on + strand at 332.214 kb on + strand at 332.215 kb on - strand at 332.215 kb on - strand at 332.316 kb on + strand at 332.316 kb on + strand at 332.316 kb on + strand at 332.316 kb on + strand at 332.316 kb on + strand at 332.316 kb on + strand at 332.316 kb on + strand at 332.316 kb on + strand at 332.316 kb on + strand at 332.316 kb on + strand at 332.316 kb on + strand at 332.316 kb on + strand at 332.316 kb on + strand at 332.316 kb on + strand at 332.316 kb on + strand at 332.316 kb on + strand at 332.316 kb on + strand at 332.316 kb on + strand at 332.316 kb on + strand at 332.316 kb on + strand at 332.316 kb on + strand at 332.316 kb on + strand at 332.316 kb on + strand at 332.316 kb on + strand at 332.316 kb on + strand at 332.316 kb on + strand at 332.317 kb on - strand at 332.317 kb on - strand at 332.317 kb on - strand at 332.317 kb on - strand at 332.317 kb on - strand at 332.317 kb on - strand at 332.317 kb on - strand at 332.317 kb on - strand at 332.317 kb on - strand at 332.317 kb on - strand at 332.317 kb on - strand at 332.332 kb on + strand at 332.374 kb on + strand at 332.374 kb on + strand at 332.375 kb on - strand at 332.375 kb on - strand at 332.405 kb on + strand at 332.441 kb on + strand at 332.441 kb on + strand at 332.442 kb on - strand at 332.442 kb on - strand at 332.458 kb on + strand at 332.458 kb on + strand at 332.459 kb on - strand at 332.503 kb on + strand, within IAI47_01520 at 332.561 kb on + strand, within IAI47_01520 at 332.561 kb on + strand, within IAI47_01520 at 332.561 kb on + strand, within IAI47_01520 at 332.561 kb on + strand, within IAI47_01520 at 332.561 kb on + strand, within IAI47_01520 at 332.561 kb on + strand, within IAI47_01520 at 332.561 kb on + strand, within IAI47_01520 at 332.562 kb on - strand, within IAI47_01520 at 332.562 kb on - strand, within IAI47_01520 at 332.562 kb on - strand, within IAI47_01520 at 332.562 kb on - strand, within IAI47_01520 at 332.562 kb on - strand, within IAI47_01520 at 332.594 kb on - strand, within IAI47_01520 at 332.594 kb on - strand, within IAI47_01520 at 332.594 kb on - strand, within IAI47_01520 at 332.594 kb on - strand, within IAI47_01520 at 332.594 kb on - strand, within IAI47_01520 at 332.594 kb on - strand, within IAI47_01520 at 332.594 kb on - strand, within IAI47_01520 at 332.726 kb on + strand, within IAI47_01520 at 332.771 kb on + strand, within IAI47_01520 at 332.771 kb on + strand, within IAI47_01520 at 332.771 kb on + strand, within IAI47_01520 at 332.772 kb on - strand, within IAI47_01520 at 332.772 kb on - strand, within IAI47_01520 at 332.772 kb on - strand, within IAI47_01520 at 332.772 kb on - strand, within IAI47_01520 at 332.772 kb on - strand, within IAI47_01520 at 332.773 kb on + strand, within IAI47_01520 at 332.773 kb on + strand, within IAI47_01520 at 332.773 kb on + strand, within IAI47_01520 at 332.773 kb on + strand, within IAI47_01520 at 332.774 kb on - strand, within IAI47_01520 at 332.774 kb on - strand, within IAI47_01520 at 332.809 kb on + strand, within IAI47_01520 at 332.809 kb on + strand, within IAI47_01520 at 332.809 kb on + strand, within IAI47_01520 at 332.809 kb on + strand, within IAI47_01520 at 332.809 kb on + strand, within IAI47_01520 at 332.809 kb on + strand, within IAI47_01520 at 332.810 kb on - strand, within IAI47_01520 at 332.884 kb on + strand, within IAI47_01520 at 332.884 kb on + strand, within IAI47_01520 at 332.884 kb on + strand, within IAI47_01520 at 332.884 kb on + strand, within IAI47_01520 at 332.885 kb on - strand, within IAI47_01520 at 332.951 kb on + strand, within IAI47_01520 at 332.952 kb on - strand, within IAI47_01520 at 332.952 kb on - strand, within IAI47_01520 at 332.983 kb on + strand at 332.983 kb on + strand at 332.998 kb on + strand at 332.999 kb on - strand at 332.999 kb on - strand at 333.057 kb on + strand at 333.084 kb on + strand at 333.084 kb on + strand at 333.085 kb on - strand at 333.085 kb on - strand at 333.098 kb on + strand at 333.099 kb on - strand at 333.141 kb on + strand at 333.141 kb on + strand at 333.141 kb on + strand at 333.142 kb on - strand at 333.142 kb on - strand at 333.142 kb on - strand at 333.142 kb on - strand at 333.142 kb on - strand at 333.142 kb on - strand at 333.142 kb on - strand at 333.142 kb on - strand at 333.142 kb on - strand at 333.148 kb on + strand at 333.149 kb on - strand at 333.159 kb on + strand at 333.186 kb on + strand at 333.186 kb on + strand at 333.186 kb on + strand at 333.186 kb on + strand at 333.187 kb on - strand at 333.187 kb on - strand at 333.187 kb on - strand at 333.187 kb on - strand at 333.210 kb on + strand at 333.210 kb on + strand at 333.210 kb on + strand at 333.210 kb on + strand at 333.211 kb on - strand at 333.258 kb on + strand, within IAI47_01525 at 333.259 kb on - strand, within IAI47_01525 at 333.259 kb on - strand, within IAI47_01525 at 333.259 kb on - strand, within IAI47_01525 at 333.328 kb on - strand, within IAI47_01525 at 333.328 kb on - strand, within IAI47_01525 at 333.397 kb on + strand, within IAI47_01525 at 333.398 kb on - strand, within IAI47_01525 at 333.398 kb on - strand, within IAI47_01525 at 333.398 kb on - strand, within IAI47_01525 at 333.398 kb on - strand, within IAI47_01525 at 333.463 kb on + strand, within IAI47_01525 at 333.464 kb on - strand, within IAI47_01525 at 333.519 kb on + strand at 333.519 kb on + strand at 333.520 kb on - strand at 333.571 kb on + strand at 333.571 kb on + strand at 333.571 kb on + strand at 333.571 kb on + strand at 333.571 kb on + strand at 333.571 kb on + strand at 333.571 kb on + strand at 333.571 kb on + strand at 333.571 kb on + strand at 333.572 kb on - strand at 333.572 kb on - strand at 333.572 kb on - strand at 333.746 kb on + strand, within IAI47_01530 at 333.747 kb on - strand, within IAI47_01530 at 333.751 kb on + strand, within IAI47_01530 at 333.751 kb on + strand, within IAI47_01530 at 333.751 kb on + strand, within IAI47_01530 at 333.751 kb on + strand, within IAI47_01530 at 333.751 kb on + strand, within IAI47_01530 at 333.752 kb on - strand, within IAI47_01530 at 333.752 kb on - strand, within IAI47_01530 at 333.752 kb on - strand, within IAI47_01530 at 333.752 kb on - strand, within IAI47_01530 at 333.752 kb on - strand, within IAI47_01530 at 333.752 kb on - strand, within IAI47_01530 at 333.785 kb on + strand, within IAI47_01530 at 333.786 kb on - strand, within IAI47_01530 at 333.810 kb on + strand, within IAI47_01530 at 333.810 kb on + strand, within IAI47_01530 at 333.810 kb on + strand, within IAI47_01530 at 333.810 kb on + strand, within IAI47_01530 at 333.810 kb on + strand, within IAI47_01530 at 333.810 kb on + strand, within IAI47_01530 at 333.811 kb on - strand, within IAI47_01530 at 333.811 kb on - strand, within IAI47_01530 at 333.811 kb on - strand, within IAI47_01530 at 333.811 kb on - strand, within IAI47_01530 at 333.811 kb on - strand, within IAI47_01530 at 333.833 kb on + strand at 333.833 kb on + strand at 333.837 kb on + strand at 333.837 kb on + strand at 333.838 kb on - strand at 333.838 kb on - strand at 333.838 kb on - strand at 333.838 kb on - strand at 333.838 kb on - strand at 333.838 kb on - strand at 333.866 kb on + strand at 333.867 kb on - strand at 333.867 kb on - strand at 333.867 kb on - strand at 333.875 kb on + strand at 333.875 kb on + strand at 333.875 kb on + strand at 333.875 kb on + strand at 333.875 kb on + strand at 333.962 kb on + strand, within IAI47_01535 at 333.962 kb on + strand, within IAI47_01535 at 333.962 kb on + strand, within IAI47_01535 at 333.963 kb on - strand, within IAI47_01535 at 333.963 kb on - strand, within IAI47_01535
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 5.0 mM Glucose; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 331,414 + IAI47_01515 0.60 -1.0 331,414 + IAI47_01515 0.60 -0.1 331,415 - IAI47_01515 0.61 +0.2 331,521 + IAI47_01515 0.65 +0.2 331,522 - IAI47_01515 0.65 -2.8 331,583 + IAI47_01515 0.68 -1.0 331,583 + IAI47_01515 0.68 -2.0 331,584 - IAI47_01515 0.68 -2.8 331,584 - IAI47_01515 0.68 -0.2 331,584 - IAI47_01515 0.68 +0.7 331,584 - IAI47_01515 0.68 -0.2 331,584 - IAI47_01515 0.68 -0.9 331,650 + IAI47_01515 0.71 -1.2 331,651 - IAI47_01515 0.71 +2.4 331,681 + IAI47_01515 0.72 -1.0 331,681 + IAI47_01515 0.72 -0.8 331,681 + IAI47_01515 0.72 -0.8 331,681 + IAI47_01515 0.72 +0.5 331,682 - IAI47_01515 0.72 +0.7 331,682 - IAI47_01515 0.72 -0.2 331,695 + IAI47_01515 0.73 +0.3 331,695 + IAI47_01515 0.73 -0.7 331,696 - IAI47_01515 0.73 -1.8 331,696 - IAI47_01515 0.73 +1.9 331,826 - IAI47_01515 0.79 -0.4 332,026 + IAI47_01515 0.87 -0.9 332,026 + IAI47_01515 0.87 -0.0 332,027 - IAI47_01515 0.87 +2.0 332,094 + -1.1 332,094 + -0.9 332,095 - -0.4 332,095 - +2.3 332,095 - -1.5 332,095 - -0.3 332,160 + -0.1 332,160 + -1.4 332,161 - -0.1 332,161 - +1.0 332,184 + -2.4 332,185 - +1.5 332,185 - -0.6 332,185 - -1.4 332,195 - +2.1 332,202 + -1.1 332,202 + -1.4 332,203 - -0.6 332,203 - -0.5 332,214 + +0.5 332,214 + -2.8 332,214 + -0.6 332,215 - +0.5 332,215 - -0.7 332,316 + -2.0 332,316 + -0.0 332,316 + +0.8 332,316 + +1.3 332,316 + +0.4 332,316 + -0.8 332,316 + -0.2 332,316 + +1.0 332,316 + -0.8 332,316 + +0.7 332,316 + -2.5 332,316 + +0.7 332,316 + -1.8 332,316 + +1.8 332,316 + +4.7 332,316 + -1.0 332,316 + +1.8 332,316 + -0.5 332,316 + +1.1 332,316 + -1.3 332,316 + -0.2 332,316 + +1.7 332,316 + -1.1 332,316 + +0.3 332,316 + -0.6 332,316 + -0.2 332,317 - -0.2 332,317 - +0.6 332,317 - +3.2 332,317 - -0.7 332,317 - -0.6 332,317 - -0.2 332,317 - -1.2 332,317 - -0.5 332,317 - -0.6 332,317 - -1.7 332,317 - -1.3 332,332 + -1.0 332,374 + -1.5 332,374 + +2.6 332,375 - -0.4 332,375 - +0.7 332,405 + -0.3 332,441 + +0.7 332,441 + +0.2 332,442 - -0.3 332,442 - +0.0 332,458 + -0.2 332,458 + -2.0 332,459 - -0.6 332,503 + IAI47_01520 0.14 -1.3 332,561 + IAI47_01520 0.24 -0.8 332,561 + IAI47_01520 0.24 +1.2 332,561 + IAI47_01520 0.24 -0.3 332,561 + IAI47_01520 0.24 -0.3 332,561 + IAI47_01520 0.24 -2.9 332,561 + IAI47_01520 0.24 -0.8 332,561 + IAI47_01520 0.24 +0.9 332,562 - IAI47_01520 0.24 +0.2 332,562 - IAI47_01520 0.24 +0.6 332,562 - IAI47_01520 0.24 -1.1 332,562 - IAI47_01520 0.24 +0.1 332,562 - IAI47_01520 0.24 -1.4 332,594 - IAI47_01520 0.29 -0.5 332,594 - IAI47_01520 0.29 -0.6 332,594 - IAI47_01520 0.29 -0.8 332,594 - IAI47_01520 0.29 +1.3 332,594 - IAI47_01520 0.29 -1.4 332,594 - IAI47_01520 0.29 -0.3 332,594 - IAI47_01520 0.29 -1.0 332,726 + IAI47_01520 0.50 -1.2 332,771 + IAI47_01520 0.57 -2.4 332,771 + IAI47_01520 0.57 +1.5 332,771 + IAI47_01520 0.57 +0.7 332,772 - IAI47_01520 0.57 -2.1 332,772 - IAI47_01520 0.57 +0.1 332,772 - IAI47_01520 0.57 +2.5 332,772 - IAI47_01520 0.57 +0.3 332,772 - IAI47_01520 0.57 -2.0 332,773 + IAI47_01520 0.57 -1.9 332,773 + IAI47_01520 0.57 -0.8 332,773 + IAI47_01520 0.57 -1.0 332,773 + IAI47_01520 0.57 -1.9 332,774 - IAI47_01520 0.57 -0.3 332,774 - IAI47_01520 0.57 +0.3 332,809 + IAI47_01520 0.63 -1.0 332,809 + IAI47_01520 0.63 -1.4 332,809 + IAI47_01520 0.63 -0.2 332,809 + IAI47_01520 0.63 -3.5 332,809 + IAI47_01520 0.63 +1.0 332,809 + IAI47_01520 0.63 -0.2 332,810 - IAI47_01520 0.63 +2.3 332,884 + IAI47_01520 0.75 -2.2 332,884 + IAI47_01520 0.75 -1.7 332,884 + IAI47_01520 0.75 -0.4 332,884 + IAI47_01520 0.75 -0.7 332,885 - IAI47_01520 0.75 +1.4 332,951 + IAI47_01520 0.86 -1.6 332,952 - IAI47_01520 0.86 +0.5 332,952 - IAI47_01520 0.86 +0.7 332,983 + -1.3 332,983 + +0.0 332,998 + -2.1 332,999 - +0.1 332,999 - -1.4 333,057 + -1.9 333,084 + -1.6 333,084 + -0.9 333,085 - +0.2 333,085 - -1.2 333,098 + -0.2 333,099 - +4.1 333,141 + +0.1 333,141 + -3.0 333,141 + -0.4 333,142 - +0.8 333,142 - -1.2 333,142 - -1.0 333,142 - -0.1 333,142 - -0.2 333,142 - +1.6 333,142 - -0.1 333,142 - -0.6 333,142 - +2.5 333,148 + +2.3 333,149 - +1.1 333,159 + +2.9 333,186 + -0.8 333,186 + -0.6 333,186 + -0.4 333,186 + +0.6 333,187 - +0.8 333,187 - +0.3 333,187 - -0.4 333,187 - -0.7 333,210 + -0.2 333,210 + +1.0 333,210 + +0.6 333,210 + +2.3 333,211 - -0.6 333,258 + IAI47_01525 0.20 -1.0 333,259 - IAI47_01525 0.20 +1.2 333,259 - IAI47_01525 0.20 +3.0 333,259 - IAI47_01525 0.20 -1.8 333,328 - IAI47_01525 0.39 +2.3 333,328 - IAI47_01525 0.39 -0.7 333,397 + IAI47_01525 0.59 -1.0 333,398 - IAI47_01525 0.59 -0.7 333,398 - IAI47_01525 0.59 -0.7 333,398 - IAI47_01525 0.59 -0.3 333,398 - IAI47_01525 0.59 +2.1 333,463 + IAI47_01525 0.77 -1.4 333,464 - IAI47_01525 0.78 -1.0 333,519 + -1.1 333,519 + -0.0 333,520 - -0.2 333,571 + -0.4 333,571 + +0.5 333,571 + -1.7 333,571 + +0.5 333,571 + -0.0 333,571 + -0.7 333,571 + -1.6 333,571 + +0.1 333,571 + -2.6 333,572 - -1.4 333,572 - -0.5 333,572 - -0.4 333,746 + IAI47_01530 0.64 -1.6 333,747 - IAI47_01530 0.65 -1.1 333,751 + IAI47_01530 0.66 -1.9 333,751 + IAI47_01530 0.66 -1.4 333,751 + IAI47_01530 0.66 -1.1 333,751 + IAI47_01530 0.66 -0.9 333,751 + IAI47_01530 0.66 -1.6 333,752 - IAI47_01530 0.67 -0.9 333,752 - IAI47_01530 0.67 +0.2 333,752 - IAI47_01530 0.67 +0.2 333,752 - IAI47_01530 0.67 -0.2 333,752 - IAI47_01530 0.67 -0.7 333,752 - IAI47_01530 0.67 -0.2 333,785 + IAI47_01530 0.79 -1.5 333,786 - IAI47_01530 0.79 -0.7 333,810 + IAI47_01530 0.88 -1.2 333,810 + IAI47_01530 0.88 -2.4 333,810 + IAI47_01530 0.88 +0.5 333,810 + IAI47_01530 0.88 -1.5 333,810 + IAI47_01530 0.88 -2.1 333,810 + IAI47_01530 0.88 -1.8 333,811 - IAI47_01530 0.88 -0.6 333,811 - IAI47_01530 0.88 -1.0 333,811 - IAI47_01530 0.88 -0.0 333,811 - IAI47_01530 0.88 -1.6 333,811 - IAI47_01530 0.88 -0.7 333,833 + -1.5 333,833 + -2.1 333,837 + -0.6 333,837 + +0.6 333,838 - -3.4 333,838 - -1.4 333,838 - -1.1 333,838 - -0.9 333,838 - -0.1 333,838 - -1.0 333,866 + -0.8 333,867 - -1.4 333,867 - +3.2 333,867 - +1.8 333,875 + -1.6 333,875 + -3.6 333,875 + -1.9 333,875 + -0.6 333,875 + -1.6 333,962 + IAI47_01535 0.22 -2.9 333,962 + IAI47_01535 0.22 -0.2 333,962 + IAI47_01535 0.22 -1.2 333,963 - IAI47_01535 0.22 +4.0 333,963 - IAI47_01535 0.22 +1.9
Or see this region's nucleotide sequence