Strain Fitness in Desulfovibrio vulgaris Hildenborough JW710 around DVU_tRNA-Ala-5

Experiment: Pyruvate-Sulfate (60-15mM) with Selenate 750uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntDVU2261 and tRNA-Ala are separated by 96 nucleotidestRNA-Ala and DVU2263 are separated by 455 nucleotides DVU2261: DVU2261 - hypothetical protein (TIGR), at 2,354,254 to 2,356,068 DVU2261 DVU_tRNA-Ala-5: tRNA-Ala - tRNA with anticodon CGC for Ala (VIMSS-AUTO), at 2,356,165 to 2,356,240 tRNA-Ala DVU2263: DVU2263 - outer membrane autotransporter barrel domain protein (TIGR), at 2,356,696 to 2,358,621 DVU2263 Position (kb) 2356 2357Strain fitness (log2 ratio) -1 0 1 2 3at 2355.174 kb on + strand, within DVU2261at 2355.181 kb on + strand, within DVU2261at 2355.181 kb on + strand, within DVU2261at 2355.208 kb on + strand, within DVU2261at 2355.239 kb on + strand, within DVU2261at 2355.298 kb on - strand, within DVU2261at 2355.338 kb on + strand, within DVU2261at 2355.442 kb on - strand, within DVU2261at 2355.502 kb on - strand, within DVU2261at 2355.535 kb on + strand, within DVU2261at 2355.577 kb on + strand, within DVU2261at 2355.601 kb on - strand, within DVU2261at 2355.606 kb on - strand, within DVU2261at 2355.621 kb on + strand, within DVU2261at 2355.621 kb on + strand, within DVU2261at 2355.784 kb on - strand, within DVU2261at 2355.887 kb on + strandat 2355.911 kb on + strandat 2355.912 kb on - strandat 2355.933 kb on - strandat 2355.950 kb on + strandat 2355.950 kb on + strandat 2356.008 kb on + strandat 2356.010 kb on + strandat 2356.016 kb on + strandat 2356.016 kb on - strandat 2356.024 kb on + strandat 2356.042 kb on + strandat 2356.056 kb on + strandat 2356.212 kb on - strand, within tRNA-Alaat 2356.238 kb on - strandat 2356.251 kb on + strandat 2356.305 kb on - strandat 2356.355 kb on + strandat 2356.375 kb on - strandat 2356.436 kb on - strandat 2356.470 kb on + strandat 2356.557 kb on + strandat 2356.727 kb on + strandat 2356.748 kb on + strandat 2356.748 kb on + strandat 2356.797 kb on + strandat 2356.808 kb on + strandat 2356.817 kb on - strandat 2356.889 kb on - strand, within DVU2263at 2356.968 kb on + strand, within DVU2263at 2356.968 kb on + strand, within DVU2263at 2357.000 kb on - strand, within DVU2263at 2357.006 kb on + strand, within DVU2263at 2357.014 kb on - strand, within DVU2263at 2357.043 kb on - strand, within DVU2263at 2357.072 kb on + strand, within DVU2263at 2357.104 kb on + strand, within DVU2263at 2357.104 kb on + strand, within DVU2263at 2357.112 kb on - strand, within DVU2263at 2357.112 kb on - strand, within DVU2263at 2357.112 kb on - strand, within DVU2263at 2357.112 kb on - strand, within DVU2263at 2357.125 kb on + strand, within DVU2263at 2357.125 kb on + strand, within DVU2263at 2357.133 kb on - strand, within DVU2263at 2357.161 kb on - strand, within DVU2263at 2357.236 kb on - strand, within DVU2263

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Per-strain Table

Position Strand Gene LocusTag Fraction Pyruvate-Sulfate (60-15mM) with Selenate 750uM
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2,355,174 + DVU2261 0.51 -0.5
2,355,181 + DVU2261 0.51 +0.4
2,355,181 + DVU2261 0.51 -0.2
2,355,208 + DVU2261 0.53 +0.3
2,355,239 + DVU2261 0.54 -0.1
2,355,298 - DVU2261 0.58 +0.4
2,355,338 + DVU2261 0.60 -0.9
2,355,442 - DVU2261 0.65 +0.7
2,355,502 - DVU2261 0.69 -0.2
2,355,535 + DVU2261 0.71 +0.2
2,355,577 + DVU2261 0.73 +0.1
2,355,601 - DVU2261 0.74 +2.0
2,355,606 - DVU2261 0.74 +0.0
2,355,621 + DVU2261 0.75 +1.4
2,355,621 + DVU2261 0.75 -0.3
2,355,784 - DVU2261 0.84 -0.2
2,355,887 + -0.4
2,355,911 + -0.1
2,355,912 - -0.2
2,355,933 - -0.3
2,355,950 + +0.0
2,355,950 + -0.3
2,356,008 + +0.3
2,356,010 + -0.1
2,356,016 + +0.5
2,356,016 - -0.2
2,356,024 + -0.3
2,356,042 + -0.7
2,356,056 + -0.0
2,356,212 - tRNA-Ala DVU_tRNA-Ala-5 0.62 +0.7
2,356,238 - +0.2
2,356,251 + +3.0
2,356,305 - +0.1
2,356,355 + -1.4
2,356,375 - -0.0
2,356,436 - -1.2
2,356,470 + -0.2
2,356,557 + +1.2
2,356,727 + +0.1
2,356,748 + -0.2
2,356,748 + -0.5
2,356,797 + -1.2
2,356,808 + +0.7
2,356,817 - +0.1
2,356,889 - DVU2263 0.10 -0.0
2,356,968 + DVU2263 0.14 -0.5
2,356,968 + DVU2263 0.14 -0.1
2,357,000 - DVU2263 0.16 -0.0
2,357,006 + DVU2263 0.16 -0.5
2,357,014 - DVU2263 0.17 -0.2
2,357,043 - DVU2263 0.18 -1.6
2,357,072 + DVU2263 0.20 +0.3
2,357,104 + DVU2263 0.21 +0.2
2,357,104 + DVU2263 0.21 -0.1
2,357,112 - DVU2263 0.22 -0.2
2,357,112 - DVU2263 0.22 +0.1
2,357,112 - DVU2263 0.22 -0.5
2,357,112 - DVU2263 0.22 -1.5
2,357,125 + DVU2263 0.22 -0.0
2,357,125 + DVU2263 0.22 +0.2
2,357,133 - DVU2263 0.23 -0.8
2,357,161 - DVU2263 0.24 -0.4
2,357,236 - DVU2263 0.28 -0.1

Or see this region's nucleotide sequence