Experiment: Pyruvate-Sulfate (60-15mM) with Selenate 750uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVUA0113 and DVUA0114 overlap by 8 nucleotides DVUA0114 and DVUA0115 are separated by 25 nucleotides DVUA0115 and DVUA0116 are separated by 194 nucleotides DVUA0116 and DORF2111 are separated by 42 nucleotides DORF2111 and escJ are separated by 24 nucleotides
DVUA0113: DVUA0113 - type III secretion protein, YscD family (TIGR), at 151,240 to 153,048
DVUA0113
DVUA0114: DVUA0114 - hypothetical protein (TIGR), at 153,041 to 153,250
DVUA0114
DVUA0115: DVUA0115 - type III secretion system protein, YscF family (TIGR), at 153,276 to 153,527
DVUA0115
DVUA0116: DVUA0116 - conserved hypothetical protein (TIGR), at 153,722 to 154,087
DVUA0116
DORF2111: DORF2111 - SEED: FIG00602408: hypothetical protein, at 154,130 to 154,945
DORF2111
DVUA0117: escJ - type III secretion lipoprotein (TIGR), at 154,970 to 155,785
escJ
Position (kb) 153
154
155 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 152.829 kb on + strand, within DVUA0113 at 152.829 kb on - strand, within DVUA0113 at 152.862 kb on + strand, within DVUA0113 at 152.879 kb on + strand at 152.887 kb on - strand at 152.893 kb on + strand at 152.893 kb on + strand at 153.027 kb on + strand at 153.134 kb on - strand, within DVUA0114 at 153.134 kb on - strand, within DVUA0114 at 153.134 kb on - strand, within DVUA0114 at 153.238 kb on - strand at 153.238 kb on - strand at 153.298 kb on + strand at 153.325 kb on - strand, within DVUA0115 at 153.327 kb on + strand, within DVUA0115 at 153.350 kb on + strand, within DVUA0115 at 153.350 kb on + strand, within DVUA0115 at 153.368 kb on - strand, within DVUA0115 at 153.393 kb on + strand, within DVUA0115 at 153.398 kb on + strand, within DVUA0115 at 153.411 kb on - strand, within DVUA0115 at 153.411 kb on - strand, within DVUA0115 at 153.411 kb on - strand, within DVUA0115 at 153.446 kb on - strand, within DVUA0115 at 153.446 kb on - strand, within DVUA0115 at 153.451 kb on - strand, within DVUA0115 at 153.451 kb on - strand, within DVUA0115 at 153.482 kb on + strand, within DVUA0115 at 153.522 kb on + strand at 153.522 kb on + strand at 153.530 kb on - strand at 153.530 kb on - strand at 153.539 kb on + strand at 153.557 kb on + strand at 153.670 kb on + strand at 153.670 kb on + strand at 153.699 kb on - strand at 153.712 kb on + strand at 153.720 kb on - strand at 153.808 kb on + strand, within DVUA0116 at 153.816 kb on - strand, within DVUA0116 at 153.871 kb on - strand, within DVUA0116 at 153.872 kb on - strand, within DVUA0116 at 153.967 kb on - strand, within DVUA0116 at 153.973 kb on + strand, within DVUA0116 at 153.974 kb on + strand, within DVUA0116 at 154.003 kb on + strand, within DVUA0116 at 154.027 kb on + strand, within DVUA0116 at 154.085 kb on - strand at 154.167 kb on + strand at 154.210 kb on + strand at 154.245 kb on + strand, within DORF2111 at 154.282 kb on + strand, within DORF2111 at 154.344 kb on + strand, within DORF2111 at 154.362 kb on - strand, within DORF2111 at 154.445 kb on + strand, within DORF2111 at 154.452 kb on + strand, within DORF2111 at 154.452 kb on + strand, within DORF2111 at 154.465 kb on + strand, within DORF2111 at 154.500 kb on + strand, within DORF2111 at 154.534 kb on + strand, within DORF2111 at 154.542 kb on - strand, within DORF2111 at 154.542 kb on - strand, within DORF2111 at 154.589 kb on + strand, within DORF2111 at 154.656 kb on - strand, within DORF2111 at 154.665 kb on + strand, within DORF2111 at 154.699 kb on - strand, within DORF2111 at 154.722 kb on - strand, within DORF2111 at 154.834 kb on + strand, within DORF2111 at 154.841 kb on + strand, within DORF2111 at 154.849 kb on - strand, within DORF2111 at 154.886 kb on + strand at 154.892 kb on - strand at 154.904 kb on + strand at 154.976 kb on - strand at 154.997 kb on + strand at 155.041 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Pyruvate-Sulfate (60-15mM) with Selenate 750uM remove 152,829 + DVUA0113 0.88 -0.2 152,829 - DVUA0113 0.88 -0.6 152,862 + DVUA0113 0.90 +0.1 152,879 + -0.9 152,887 - +1.6 152,893 + -0.2 152,893 + -0.1 153,027 + +1.0 153,134 - DVUA0114 0.44 -1.3 153,134 - DVUA0114 0.44 -0.7 153,134 - DVUA0114 0.44 +1.9 153,238 - -0.9 153,238 - -0.3 153,298 + -0.1 153,325 - DVUA0115 0.19 +0.1 153,327 + DVUA0115 0.20 -0.3 153,350 + DVUA0115 0.29 -0.4 153,350 + DVUA0115 0.29 -4.2 153,368 - DVUA0115 0.37 -0.0 153,393 + DVUA0115 0.46 -0.8 153,398 + DVUA0115 0.48 -0.1 153,411 - DVUA0115 0.54 -0.7 153,411 - DVUA0115 0.54 -0.4 153,411 - DVUA0115 0.54 -0.7 153,446 - DVUA0115 0.67 -0.4 153,446 - DVUA0115 0.67 +0.4 153,451 - DVUA0115 0.69 -0.3 153,451 - DVUA0115 0.69 +0.9 153,482 + DVUA0115 0.82 -0.2 153,522 + -0.5 153,522 + +0.6 153,530 - -0.9 153,530 - +1.2 153,539 + +0.0 153,557 + -0.0 153,670 + +0.0 153,670 + +0.0 153,699 - +0.3 153,712 + +0.2 153,720 - -0.8 153,808 + DVUA0116 0.23 +0.6 153,816 - DVUA0116 0.26 -0.3 153,871 - DVUA0116 0.41 -0.0 153,872 - DVUA0116 0.41 -0.7 153,967 - DVUA0116 0.67 +0.1 153,973 + DVUA0116 0.69 +0.5 153,974 + DVUA0116 0.69 +1.0 154,003 + DVUA0116 0.77 +1.8 154,027 + DVUA0116 0.83 -0.2 154,085 - -2.2 154,167 + -0.8 154,210 + +0.1 154,245 + DORF2111 0.14 +0.3 154,282 + DORF2111 0.19 -0.6 154,344 + DORF2111 0.26 -0.1 154,362 - DORF2111 0.28 -0.2 154,445 + DORF2111 0.39 -0.2 154,452 + DORF2111 0.39 -0.3 154,452 + DORF2111 0.39 -0.4 154,465 + DORF2111 0.41 -1.1 154,500 + DORF2111 0.45 -0.4 154,534 + DORF2111 0.50 -1.3 154,542 - DORF2111 0.50 -0.1 154,542 - DORF2111 0.50 -2.5 154,589 + DORF2111 0.56 -1.5 154,656 - DORF2111 0.64 -0.3 154,665 + DORF2111 0.66 -0.5 154,699 - DORF2111 0.70 +0.2 154,722 - DORF2111 0.73 +1.5 154,834 + DORF2111 0.86 +0.9 154,841 + DORF2111 0.87 -0.2 154,849 - DORF2111 0.88 -2.4 154,886 + -0.6 154,892 - +0.2 154,904 + +0.1 154,976 - -0.2 154,997 + +0.6 155,041 - +0.5
Or see this region's nucleotide sequence