Experiment: Pyruvate-Sulfate (60-15mM) with Selenate 750uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pyrD and DVU3318 are separated by 183 nucleotides DVU3318 and putA are separated by 94 nucleotides
DVU3316: pyrD - dihydroorotate dehydrogenase (TIGR), at 3,493,924 to 3,494,847
pyrD
DVU3318: DVU3318 - hypothetical protein (TIGR), at 3,495,031 to 3,495,201
DVU3318
DVU3319: putA - proline dehydrogenase/delta-1-pyrroline-5-carboxylate dehydrogenase (TIGR), at 3,495,296 to 3,498,316
putA
Position (kb)
3495
3496 Strain fitness (log2 ratio)
-1
0
1
2 at 3494.842 kb on + strand at 3495.047 kb on - strand at 3495.075 kb on - strand, within DVU3318 at 3495.098 kb on - strand, within DVU3318 at 3495.117 kb on - strand, within DVU3318 at 3495.118 kb on - strand, within DVU3318 at 3495.146 kb on - strand, within DVU3318 at 3495.182 kb on - strand, within DVU3318 at 3495.230 kb on + strand at 3495.230 kb on + strand at 3495.230 kb on + strand at 3495.238 kb on - strand at 3495.307 kb on - strand at 3495.355 kb on + strand at 3495.396 kb on + strand at 3495.406 kb on + strand at 3495.407 kb on + strand at 3495.407 kb on + strand at 3495.415 kb on - strand at 3495.415 kb on - strand at 3495.417 kb on + strand at 3495.420 kb on - strand at 3495.459 kb on - strand at 3495.472 kb on + strand at 3495.478 kb on + strand at 3495.485 kb on - strand at 3495.494 kb on - strand at 3495.525 kb on + strand at 3495.604 kb on + strand, within putA at 3495.604 kb on + strand, within putA at 3495.607 kb on + strand, within putA at 3495.618 kb on - strand, within putA at 3495.688 kb on - strand, within putA at 3495.797 kb on - strand, within putA at 3495.871 kb on - strand, within putA at 3495.917 kb on - strand, within putA at 3495.936 kb on - strand, within putA at 3496.044 kb on + strand, within putA at 3496.086 kb on - strand, within putA at 3496.161 kb on + strand, within putA at 3496.177 kb on - strand, within putA
Per-strain Table
Position Strand Gene LocusTag Fraction Pyruvate-Sulfate (60-15mM) with Selenate 750uM remove 3,494,842 + +0.1 3,495,047 - -0.2 3,495,075 - DVU3318 0.26 +2.0 3,495,098 - DVU3318 0.39 -0.0 3,495,117 - DVU3318 0.50 -0.8 3,495,118 - DVU3318 0.51 -0.2 3,495,146 - DVU3318 0.67 +0.3 3,495,182 - DVU3318 0.88 -0.1 3,495,230 + +0.5 3,495,230 + +0.3 3,495,230 + -0.3 3,495,238 - -0.0 3,495,307 - -0.2 3,495,355 + -1.1 3,495,396 + +0.5 3,495,406 + -0.2 3,495,407 + +0.3 3,495,407 + -0.1 3,495,415 - +0.1 3,495,415 - +0.2 3,495,417 + -0.0 3,495,420 - +0.3 3,495,459 - -0.4 3,495,472 + -0.6 3,495,478 + +0.5 3,495,485 - +0.4 3,495,494 - -0.0 3,495,525 + -0.3 3,495,604 + putA DVU3319 0.10 -1.1 3,495,604 + putA DVU3319 0.10 -0.0 3,495,607 + putA DVU3319 0.10 -0.2 3,495,618 - putA DVU3319 0.11 +0.1 3,495,688 - putA DVU3319 0.13 -0.1 3,495,797 - putA DVU3319 0.17 -0.5 3,495,871 - putA DVU3319 0.19 +0.1 3,495,917 - putA DVU3319 0.21 +0.2 3,495,936 - putA DVU3319 0.21 +0.0 3,496,044 + putA DVU3319 0.25 -0.1 3,496,086 - putA DVU3319 0.26 -0.6 3,496,161 + putA DVU3319 0.29 -0.0 3,496,177 - putA DVU3319 0.29 +0.3
Or see this region's nucleotide sequence