Experiment: Calcium chloride 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_945 and Pf6N2E2_946 are separated by 172 nucleotides Pf6N2E2_946 and Pf6N2E2_947 are separated by 140 nucleotides
Pf6N2E2_945 - Transcriptional regulator, TetR family, at 1,113,032 to 1,113,610
_945
Pf6N2E2_946 - Transcriptional regulator, LysR family, at 1,113,783 to 1,114,061
_946
Pf6N2E2_947 - Multidrug efflux membrane fusion protein MexE, at 1,114,202 to 1,115,434
_947
Position (kb)
1113
1114
1115 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1112.798 kb on - strand at 1112.915 kb on + strand at 1112.915 kb on + strand at 1112.915 kb on + strand at 1112.920 kb on + strand at 1112.921 kb on - strand at 1112.999 kb on + strand at 1112.999 kb on + strand at 1113.064 kb on + strand at 1113.329 kb on - strand, within Pf6N2E2_945 at 1113.437 kb on + strand, within Pf6N2E2_945 at 1113.468 kb on + strand, within Pf6N2E2_945 at 1113.792 kb on - strand at 1113.854 kb on + strand, within Pf6N2E2_946 at 1113.854 kb on + strand, within Pf6N2E2_946 at 1113.855 kb on - strand, within Pf6N2E2_946 at 1113.855 kb on - strand, within Pf6N2E2_946 at 1113.855 kb on - strand, within Pf6N2E2_946 at 1113.881 kb on - strand, within Pf6N2E2_946 at 1114.034 kb on + strand at 1114.059 kb on + strand at 1114.060 kb on - strand at 1114.060 kb on - strand at 1114.115 kb on + strand at 1114.197 kb on + strand at 1114.197 kb on + strand at 1114.197 kb on + strand at 1114.197 kb on + strand at 1114.197 kb on + strand at 1114.201 kb on + strand at 1114.201 kb on + strand at 1114.201 kb on + strand at 1114.202 kb on - strand at 1114.202 kb on - strand at 1114.254 kb on + strand at 1114.254 kb on + strand at 1114.775 kb on - strand, within Pf6N2E2_947 at 1114.836 kb on - strand, within Pf6N2E2_947 at 1114.836 kb on - strand, within Pf6N2E2_947 at 1114.836 kb on - strand, within Pf6N2E2_947 at 1114.916 kb on + strand, within Pf6N2E2_947 at 1114.916 kb on + strand, within Pf6N2E2_947 at 1114.917 kb on - strand, within Pf6N2E2_947 at 1114.917 kb on - strand, within Pf6N2E2_947 at 1114.917 kb on - strand, within Pf6N2E2_947 at 1114.917 kb on - strand, within Pf6N2E2_947 at 1115.042 kb on + strand, within Pf6N2E2_947 at 1115.042 kb on + strand, within Pf6N2E2_947
Per-strain Table
Position Strand Gene LocusTag Fraction Calcium chloride 0.0125 M remove 1,112,798 - +0.6 1,112,915 + -0.6 1,112,915 + -0.7 1,112,915 + -0.3 1,112,920 + +2.2 1,112,921 - +0.5 1,112,999 + +0.7 1,112,999 + +0.1 1,113,064 + -1.9 1,113,329 - Pf6N2E2_945 0.51 +1.0 1,113,437 + Pf6N2E2_945 0.70 -0.1 1,113,468 + Pf6N2E2_945 0.75 +0.6 1,113,792 - -0.1 1,113,854 + Pf6N2E2_946 0.25 -2.2 1,113,854 + Pf6N2E2_946 0.25 -0.2 1,113,855 - Pf6N2E2_946 0.26 -0.3 1,113,855 - Pf6N2E2_946 0.26 +0.4 1,113,855 - Pf6N2E2_946 0.26 -1.8 1,113,881 - Pf6N2E2_946 0.35 -0.6 1,114,034 + +1.2 1,114,059 + -0.1 1,114,060 - +0.8 1,114,060 - -1.4 1,114,115 + -2.1 1,114,197 + +0.5 1,114,197 + -0.0 1,114,197 + -0.7 1,114,197 + +0.0 1,114,197 + -1.2 1,114,201 + -0.4 1,114,201 + +0.2 1,114,201 + +0.1 1,114,202 - -0.2 1,114,202 - -1.8 1,114,254 + -0.6 1,114,254 + +0.1 1,114,775 - Pf6N2E2_947 0.46 -0.2 1,114,836 - Pf6N2E2_947 0.51 -0.3 1,114,836 - Pf6N2E2_947 0.51 +0.8 1,114,836 - Pf6N2E2_947 0.51 +0.3 1,114,916 + Pf6N2E2_947 0.58 -0.3 1,114,916 + Pf6N2E2_947 0.58 +0.6 1,114,917 - Pf6N2E2_947 0.58 +0.2 1,114,917 - Pf6N2E2_947 0.58 +1.5 1,114,917 - Pf6N2E2_947 0.58 +0.2 1,114,917 - Pf6N2E2_947 0.58 -0.4 1,115,042 + Pf6N2E2_947 0.68 -0.0 1,115,042 + Pf6N2E2_947 0.68 +0.4
Or see this region's nucleotide sequence