Experiment: Calcium chloride 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5542 and Pf6N2E2_5543 are separated by 52 nucleotides Pf6N2E2_5543 and Pf6N2E2_5544 are separated by 81 nucleotides Pf6N2E2_5544 and Pf6N2E2_5545 are separated by 166 nucleotides Pf6N2E2_5545 and Pf6N2E2_5546 are separated by 1 nucleotides
Pf6N2E2_5542 - tolB protein precursor, periplasmic protein involved in the tonb-independent uptake of group A colicins, at 6,357,030 to 6,358,310
_5542
Pf6N2E2_5543 - 18K peptidoglycan-associated outer membrane lipoprotein; Peptidoglycan-associated lipoprotein precursor; Outer membrane protein P6; OmpA/MotB precursor, at 6,358,363 to 6,358,860
_5543
Pf6N2E2_5544 - TPR repeat containing exported protein; Putative periplasmic protein contains a protein prenylyltransferase domain, at 6,358,942 to 6,359,706
_5544
Pf6N2E2_5545 - Queuosine Biosynthesis QueE Radical SAM, at 6,359,873 to 6,360,520
_5545
Pf6N2E2_5546 - Queuosine Biosynthesis QueC ATPase, at 6,360,522 to 6,361,214
_5546
Position (kb)
6358
6359
6360 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 6358.299 kb on + strand at 6358.319 kb on + strand at 6358.321 kb on + strand at 6358.321 kb on + strand at 6358.321 kb on + strand at 6358.321 kb on + strand at 6358.328 kb on + strand at 6358.328 kb on + strand at 6358.332 kb on + strand at 6358.350 kb on + strand at 6358.350 kb on + strand at 6358.858 kb on + strand at 6358.858 kb on + strand at 6358.858 kb on + strand at 6358.889 kb on + strand at 6358.965 kb on + strand at 6358.965 kb on + strand at 6358.980 kb on + strand at 6359.192 kb on + strand, within Pf6N2E2_5544 at 6359.193 kb on - strand, within Pf6N2E2_5544 at 6359.350 kb on + strand, within Pf6N2E2_5544 at 6359.353 kb on + strand, within Pf6N2E2_5544 at 6359.353 kb on + strand, within Pf6N2E2_5544 at 6359.353 kb on + strand, within Pf6N2E2_5544 at 6359.353 kb on + strand, within Pf6N2E2_5544 at 6359.353 kb on + strand, within Pf6N2E2_5544 at 6359.354 kb on - strand, within Pf6N2E2_5544 at 6359.431 kb on + strand, within Pf6N2E2_5544 at 6359.431 kb on + strand, within Pf6N2E2_5544 at 6359.431 kb on + strand, within Pf6N2E2_5544 at 6359.464 kb on + strand, within Pf6N2E2_5544 at 6359.543 kb on - strand, within Pf6N2E2_5544 at 6359.543 kb on - strand, within Pf6N2E2_5544 at 6359.543 kb on - strand, within Pf6N2E2_5544 at 6359.543 kb on - strand, within Pf6N2E2_5544 at 6359.575 kb on + strand, within Pf6N2E2_5544 at 6359.591 kb on + strand, within Pf6N2E2_5544 at 6359.621 kb on + strand, within Pf6N2E2_5544 at 6359.621 kb on + strand, within Pf6N2E2_5544 at 6359.621 kb on + strand, within Pf6N2E2_5544 at 6359.621 kb on + strand, within Pf6N2E2_5544 at 6359.622 kb on - strand, within Pf6N2E2_5544 at 6359.622 kb on - strand, within Pf6N2E2_5544 at 6359.622 kb on - strand, within Pf6N2E2_5544 at 6359.704 kb on + strand at 6359.704 kb on + strand at 6359.704 kb on + strand at 6359.704 kb on + strand at 6359.705 kb on - strand at 6359.705 kb on - strand at 6359.777 kb on - strand at 6359.850 kb on + strand at 6359.857 kb on + strand at 6359.858 kb on - strand at 6359.873 kb on - strand at 6359.949 kb on + strand, within Pf6N2E2_5545 at 6359.950 kb on - strand, within Pf6N2E2_5545 at 6359.950 kb on - strand, within Pf6N2E2_5545 at 6359.987 kb on + strand, within Pf6N2E2_5545 at 6360.063 kb on - strand, within Pf6N2E2_5545 at 6360.074 kb on + strand, within Pf6N2E2_5545 at 6360.075 kb on - strand, within Pf6N2E2_5545 at 6360.188 kb on - strand, within Pf6N2E2_5545 at 6360.362 kb on + strand, within Pf6N2E2_5545 at 6360.487 kb on + strand at 6360.487 kb on + strand at 6360.487 kb on + strand at 6360.488 kb on - strand at 6360.488 kb on - strand at 6360.491 kb on + strand at 6360.491 kb on + strand at 6360.491 kb on + strand at 6360.492 kb on - strand at 6360.521 kb on + strand at 6360.521 kb on + strand at 6360.522 kb on - strand at 6360.522 kb on - strand at 6360.631 kb on - strand, within Pf6N2E2_5546 at 6360.631 kb on - strand, within Pf6N2E2_5546 at 6360.631 kb on - strand, within Pf6N2E2_5546 at 6360.649 kb on - strand, within Pf6N2E2_5546
Per-strain Table
Position Strand Gene LocusTag Fraction Calcium chloride 0.0125 M remove 6,358,299 + +1.1 6,358,319 + -0.0 6,358,321 + -0.2 6,358,321 + +0.1 6,358,321 + +0.1 6,358,321 + +0.2 6,358,328 + +1.4 6,358,328 + +0.4 6,358,332 + +0.1 6,358,350 + -2.9 6,358,350 + -0.4 6,358,858 + -3.5 6,358,858 + -2.5 6,358,858 + -3.2 6,358,889 + -2.1 6,358,965 + -1.7 6,358,965 + -1.3 6,358,980 + -2.6 6,359,192 + Pf6N2E2_5544 0.33 -2.5 6,359,193 - Pf6N2E2_5544 0.33 -2.8 6,359,350 + Pf6N2E2_5544 0.53 -1.0 6,359,353 + Pf6N2E2_5544 0.54 -2.1 6,359,353 + Pf6N2E2_5544 0.54 -1.3 6,359,353 + Pf6N2E2_5544 0.54 -3.0 6,359,353 + Pf6N2E2_5544 0.54 -1.0 6,359,353 + Pf6N2E2_5544 0.54 -3.4 6,359,354 - Pf6N2E2_5544 0.54 -0.5 6,359,431 + Pf6N2E2_5544 0.64 -2.8 6,359,431 + Pf6N2E2_5544 0.64 -2.5 6,359,431 + Pf6N2E2_5544 0.64 -2.8 6,359,464 + Pf6N2E2_5544 0.68 -3.8 6,359,543 - Pf6N2E2_5544 0.79 -1.8 6,359,543 - Pf6N2E2_5544 0.79 -1.9 6,359,543 - Pf6N2E2_5544 0.79 -1.4 6,359,543 - Pf6N2E2_5544 0.79 -1.9 6,359,575 + Pf6N2E2_5544 0.83 -0.3 6,359,591 + Pf6N2E2_5544 0.85 -2.9 6,359,621 + Pf6N2E2_5544 0.89 -0.6 6,359,621 + Pf6N2E2_5544 0.89 -2.4 6,359,621 + Pf6N2E2_5544 0.89 -1.6 6,359,621 + Pf6N2E2_5544 0.89 -2.1 6,359,622 - Pf6N2E2_5544 0.89 +0.1 6,359,622 - Pf6N2E2_5544 0.89 -1.9 6,359,622 - Pf6N2E2_5544 0.89 -1.2 6,359,704 + -0.7 6,359,704 + +0.5 6,359,704 + +0.8 6,359,704 + +0.7 6,359,705 - -2.6 6,359,705 - +0.9 6,359,777 - +1.8 6,359,850 + +0.3 6,359,857 + -0.3 6,359,858 - +0.7 6,359,873 - -0.7 6,359,949 + Pf6N2E2_5545 0.12 -0.3 6,359,950 - Pf6N2E2_5545 0.12 +0.6 6,359,950 - Pf6N2E2_5545 0.12 -0.3 6,359,987 + Pf6N2E2_5545 0.18 +0.5 6,360,063 - Pf6N2E2_5545 0.29 -0.1 6,360,074 + Pf6N2E2_5545 0.31 -0.0 6,360,075 - Pf6N2E2_5545 0.31 -0.0 6,360,188 - Pf6N2E2_5545 0.49 -0.1 6,360,362 + Pf6N2E2_5545 0.75 +0.6 6,360,487 + -0.9 6,360,487 + +0.3 6,360,487 + -1.5 6,360,488 - +0.3 6,360,488 - +0.7 6,360,491 + +1.0 6,360,491 + +0.4 6,360,491 + -0.9 6,360,492 - +0.3 6,360,521 + +0.1 6,360,521 + +0.8 6,360,522 - -1.4 6,360,522 - -0.1 6,360,631 - Pf6N2E2_5546 0.16 -0.1 6,360,631 - Pf6N2E2_5546 0.16 +0.2 6,360,631 - Pf6N2E2_5546 0.16 -1.3 6,360,649 - Pf6N2E2_5546 0.18 +1.8
Or see this region's nucleotide sequence