Experiment: Calcium chloride 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_38 and Pf6N2E2_39 are separated by 143 nucleotides Pf6N2E2_39 and Pf6N2E2_40 overlap by 1 nucleotides Pf6N2E2_40 and Pf6N2E2_41 overlap by 1 nucleotides Pf6N2E2_41 and Pf6N2E2_42 overlap by 4 nucleotides
Pf6N2E2_38 - FIG00954456: hypothetical protein, at 77,278 to 78,357
_38
Pf6N2E2_39 - FIG00857858: hypothetical protein, at 78,501 to 78,809
_39
Pf6N2E2_40 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 78,809 to 79,120
_40
Pf6N2E2_41 - Two-component system response regulator QseB, at 79,120 to 79,788
_41
Pf6N2E2_42 - Sensor protein PhoQ (EC 2.7.13.3), at 79,785 to 81,098
_42
Position (kb) 79
80 Strain fitness (log2 ratio) -2
-1
0
1 at 78.388 kb on + strand at 78.389 kb on - strand at 78.389 kb on - strand at 78.389 kb on - strand at 78.390 kb on + strand at 78.391 kb on - strand at 78.409 kb on + strand at 78.410 kb on - strand at 78.410 kb on - strand at 78.410 kb on - strand at 78.410 kb on - strand at 78.482 kb on + strand at 78.483 kb on - strand at 78.483 kb on - strand at 78.484 kb on + strand at 78.484 kb on + strand at 78.484 kb on + strand at 78.485 kb on - strand at 78.485 kb on - strand at 78.485 kb on - strand at 78.485 kb on - strand at 78.485 kb on - strand at 78.485 kb on - strand at 78.485 kb on - strand at 78.495 kb on + strand at 78.611 kb on + strand, within Pf6N2E2_39 at 78.708 kb on + strand, within Pf6N2E2_39 at 78.708 kb on + strand, within Pf6N2E2_39 at 78.709 kb on - strand, within Pf6N2E2_39 at 78.709 kb on - strand, within Pf6N2E2_39 at 78.714 kb on + strand, within Pf6N2E2_39 at 78.714 kb on + strand, within Pf6N2E2_39 at 78.715 kb on - strand, within Pf6N2E2_39 at 78.715 kb on - strand, within Pf6N2E2_39 at 78.735 kb on - strand, within Pf6N2E2_39 at 78.804 kb on - strand at 78.807 kb on + strand at 78.808 kb on - strand at 78.808 kb on - strand at 78.833 kb on - strand at 79.023 kb on - strand, within Pf6N2E2_40 at 79.023 kb on - strand, within Pf6N2E2_40 at 79.023 kb on - strand, within Pf6N2E2_40 at 79.025 kb on - strand, within Pf6N2E2_40 at 79.025 kb on - strand, within Pf6N2E2_40 at 79.025 kb on - strand, within Pf6N2E2_40 at 79.029 kb on - strand, within Pf6N2E2_40 at 79.099 kb on - strand at 79.108 kb on + strand at 79.109 kb on - strand at 79.230 kb on - strand, within Pf6N2E2_41 at 79.232 kb on - strand, within Pf6N2E2_41 at 79.252 kb on + strand, within Pf6N2E2_41 at 79.253 kb on - strand, within Pf6N2E2_41 at 79.411 kb on + strand, within Pf6N2E2_41 at 79.600 kb on + strand, within Pf6N2E2_41 at 79.600 kb on + strand, within Pf6N2E2_41 at 79.601 kb on - strand, within Pf6N2E2_41 at 79.601 kb on - strand, within Pf6N2E2_41 at 79.632 kb on + strand, within Pf6N2E2_41 at 79.633 kb on - strand, within Pf6N2E2_41 at 79.658 kb on - strand, within Pf6N2E2_41 at 79.658 kb on - strand, within Pf6N2E2_41 at 79.999 kb on - strand, within Pf6N2E2_42 at 80.023 kb on - strand, within Pf6N2E2_42 at 80.023 kb on - strand, within Pf6N2E2_42 at 80.023 kb on - strand, within Pf6N2E2_42 at 80.211 kb on + strand, within Pf6N2E2_42 at 80.212 kb on - strand, within Pf6N2E2_42 at 80.212 kb on - strand, within Pf6N2E2_42 at 80.278 kb on + strand, within Pf6N2E2_42 at 80.748 kb on + strand, within Pf6N2E2_42 at 80.749 kb on - strand, within Pf6N2E2_42
Per-strain Table
Position Strand Gene LocusTag Fraction Calcium chloride 0.0125 M remove 78,388 + -0.3 78,389 - -0.0 78,389 - -0.0 78,389 - +1.0 78,390 + -1.0 78,391 - -0.5 78,409 + +1.0 78,410 - -0.7 78,410 - +0.4 78,410 - -1.1 78,410 - -2.2 78,482 + +0.8 78,483 - -0.4 78,483 - -0.1 78,484 + -1.2 78,484 + +0.5 78,484 + +1.1 78,485 - -1.6 78,485 - -0.8 78,485 - -1.2 78,485 - -0.5 78,485 - -1.2 78,485 - -1.1 78,485 - +0.5 78,495 + +0.6 78,611 + Pf6N2E2_39 0.36 -0.8 78,708 + Pf6N2E2_39 0.67 -1.7 78,708 + Pf6N2E2_39 0.67 -0.8 78,709 - Pf6N2E2_39 0.67 +0.1 78,709 - Pf6N2E2_39 0.67 +0.2 78,714 + Pf6N2E2_39 0.69 -2.0 78,714 + Pf6N2E2_39 0.69 +0.4 78,715 - Pf6N2E2_39 0.69 -0.4 78,715 - Pf6N2E2_39 0.69 -2.1 78,735 - Pf6N2E2_39 0.76 +0.5 78,804 - +0.4 78,807 + -2.2 78,808 - +1.0 78,808 - -0.8 78,833 - +0.2 79,023 - Pf6N2E2_40 0.69 -0.1 79,023 - Pf6N2E2_40 0.69 -0.8 79,023 - Pf6N2E2_40 0.69 +0.0 79,025 - Pf6N2E2_40 0.69 +0.3 79,025 - Pf6N2E2_40 0.69 +0.2 79,025 - Pf6N2E2_40 0.69 -0.7 79,029 - Pf6N2E2_40 0.71 -0.1 79,099 - +1.5 79,108 + +0.6 79,109 - +0.2 79,230 - Pf6N2E2_41 0.16 -0.1 79,232 - Pf6N2E2_41 0.17 +0.1 79,252 + Pf6N2E2_41 0.20 +0.6 79,253 - Pf6N2E2_41 0.20 +0.2 79,411 + Pf6N2E2_41 0.43 +0.7 79,600 + Pf6N2E2_41 0.72 +1.2 79,600 + Pf6N2E2_41 0.72 +0.7 79,601 - Pf6N2E2_41 0.72 +0.7 79,601 - Pf6N2E2_41 0.72 +1.4 79,632 + Pf6N2E2_41 0.77 +1.2 79,633 - Pf6N2E2_41 0.77 +0.6 79,658 - Pf6N2E2_41 0.80 -0.0 79,658 - Pf6N2E2_41 0.80 -0.2 79,999 - Pf6N2E2_42 0.16 +0.2 80,023 - Pf6N2E2_42 0.18 -0.1 80,023 - Pf6N2E2_42 0.18 +0.6 80,023 - Pf6N2E2_42 0.18 -1.2 80,211 + Pf6N2E2_42 0.32 -0.1 80,212 - Pf6N2E2_42 0.32 +0.8 80,212 - Pf6N2E2_42 0.32 -0.6 80,278 + Pf6N2E2_42 0.38 -0.0 80,748 + Pf6N2E2_42 0.73 +0.8 80,749 - Pf6N2E2_42 0.73 +0.4
Or see this region's nucleotide sequence