Experiment: Calcium chloride 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3139 and Pf6N2E2_3140 are separated by 234 nucleotides Pf6N2E2_3140 and Pf6N2E2_3141 are separated by 184 nucleotides
Pf6N2E2_3139 - L-carnitine dehydratase/bile acid-inducible protein F (EC 2.8.3.16), at 3,631,255 to 3,632,454
_3139
Pf6N2E2_3140 - FIG00953680: hypothetical protein, at 3,632,689 to 3,632,976
_3140
Pf6N2E2_3141 - virulence sensor protein, at 3,633,161 to 3,634,531
_3141
Position (kb)
3632
3633 Strain fitness (log2 ratio)
-1
0
1
2 at 3631.712 kb on - strand, within Pf6N2E2_3139 at 3631.864 kb on + strand, within Pf6N2E2_3139 at 3631.865 kb on - strand, within Pf6N2E2_3139 at 3631.865 kb on - strand, within Pf6N2E2_3139 at 3632.125 kb on + strand, within Pf6N2E2_3139 at 3632.126 kb on - strand, within Pf6N2E2_3139 at 3632.229 kb on + strand, within Pf6N2E2_3139 at 3632.229 kb on + strand, within Pf6N2E2_3139 at 3632.230 kb on - strand, within Pf6N2E2_3139 at 3632.522 kb on + strand at 3632.664 kb on + strand at 3632.665 kb on - strand at 3632.665 kb on - strand at 3632.665 kb on - strand at 3632.665 kb on - strand at 3632.776 kb on + strand, within Pf6N2E2_3140 at 3632.777 kb on - strand, within Pf6N2E2_3140 at 3632.777 kb on - strand, within Pf6N2E2_3140 at 3632.777 kb on - strand, within Pf6N2E2_3140 at 3632.934 kb on - strand, within Pf6N2E2_3140 at 3632.959 kb on + strand at 3632.959 kb on + strand at 3632.960 kb on - strand at 3632.967 kb on + strand at 3632.993 kb on - strand at 3633.020 kb on + strand at 3633.021 kb on - strand at 3633.021 kb on - strand at 3633.021 kb on - strand at 3633.021 kb on - strand at 3633.021 kb on - strand at 3633.021 kb on - strand at 3633.021 kb on - strand at 3633.106 kb on + strand at 3633.106 kb on + strand at 3633.106 kb on + strand at 3633.106 kb on + strand at 3633.106 kb on + strand at 3633.249 kb on + strand at 3633.249 kb on + strand at 3633.250 kb on - strand at 3633.250 kb on - strand at 3633.250 kb on - strand at 3633.250 kb on - strand at 3633.270 kb on + strand at 3633.271 kb on - strand at 3633.271 kb on - strand at 3633.271 kb on - strand at 3633.438 kb on - strand, within Pf6N2E2_3141 at 3633.451 kb on - strand, within Pf6N2E2_3141 at 3633.587 kb on + strand, within Pf6N2E2_3141 at 3633.588 kb on - strand, within Pf6N2E2_3141 at 3633.588 kb on - strand, within Pf6N2E2_3141 at 3633.588 kb on - strand, within Pf6N2E2_3141 at 3633.608 kb on + strand, within Pf6N2E2_3141 at 3633.609 kb on - strand, within Pf6N2E2_3141 at 3633.816 kb on + strand, within Pf6N2E2_3141 at 3633.817 kb on - strand, within Pf6N2E2_3141 at 3633.817 kb on - strand, within Pf6N2E2_3141 at 3633.817 kb on - strand, within Pf6N2E2_3141 at 3633.851 kb on + strand, within Pf6N2E2_3141 at 3633.851 kb on + strand, within Pf6N2E2_3141 at 3633.851 kb on + strand, within Pf6N2E2_3141 at 3633.852 kb on - strand, within Pf6N2E2_3141 at 3633.852 kb on - strand, within Pf6N2E2_3141 at 3633.874 kb on - strand, within Pf6N2E2_3141 at 3633.909 kb on + strand, within Pf6N2E2_3141 at 3633.909 kb on + strand, within Pf6N2E2_3141 at 3633.909 kb on + strand, within Pf6N2E2_3141 at 3633.910 kb on - strand, within Pf6N2E2_3141 at 3633.910 kb on - strand, within Pf6N2E2_3141 at 3633.948 kb on + strand, within Pf6N2E2_3141 at 3633.948 kb on + strand, within Pf6N2E2_3141 at 3633.949 kb on - strand, within Pf6N2E2_3141 at 3633.949 kb on - strand, within Pf6N2E2_3141 at 3633.949 kb on - strand, within Pf6N2E2_3141 at 3633.949 kb on - strand, within Pf6N2E2_3141 at 3633.949 kb on - strand, within Pf6N2E2_3141
Per-strain Table
Position Strand Gene LocusTag Fraction Calcium chloride 0.0125 M remove 3,631,712 - Pf6N2E2_3139 0.38 +0.6 3,631,864 + Pf6N2E2_3139 0.51 +0.7 3,631,865 - Pf6N2E2_3139 0.51 -1.6 3,631,865 - Pf6N2E2_3139 0.51 -0.5 3,632,125 + Pf6N2E2_3139 0.72 -0.9 3,632,126 - Pf6N2E2_3139 0.73 -0.0 3,632,229 + Pf6N2E2_3139 0.81 +0.9 3,632,229 + Pf6N2E2_3139 0.81 -1.0 3,632,230 - Pf6N2E2_3139 0.81 +0.3 3,632,522 + -0.1 3,632,664 + +1.6 3,632,665 - -0.7 3,632,665 - -0.4 3,632,665 - -0.7 3,632,665 - -1.4 3,632,776 + Pf6N2E2_3140 0.30 -0.8 3,632,777 - Pf6N2E2_3140 0.31 -0.9 3,632,777 - Pf6N2E2_3140 0.31 -0.5 3,632,777 - Pf6N2E2_3140 0.31 -1.5 3,632,934 - Pf6N2E2_3140 0.85 -0.1 3,632,959 + +1.4 3,632,959 + -0.2 3,632,960 - -0.2 3,632,967 + +0.6 3,632,993 - -0.4 3,633,020 + -0.3 3,633,021 - -0.4 3,633,021 - +0.2 3,633,021 - +0.2 3,633,021 - -0.3 3,633,021 - +1.6 3,633,021 - -0.3 3,633,021 - -0.3 3,633,106 + -0.8 3,633,106 + +0.3 3,633,106 + -0.1 3,633,106 + -0.2 3,633,106 + +0.1 3,633,249 + +0.1 3,633,249 + -1.5 3,633,250 - -0.5 3,633,250 - +1.7 3,633,250 - +1.1 3,633,250 - -0.6 3,633,270 + +0.9 3,633,271 - +0.2 3,633,271 - -1.2 3,633,271 - -0.9 3,633,438 - Pf6N2E2_3141 0.20 -0.2 3,633,451 - Pf6N2E2_3141 0.21 +0.5 3,633,587 + Pf6N2E2_3141 0.31 +0.1 3,633,588 - Pf6N2E2_3141 0.31 +0.6 3,633,588 - Pf6N2E2_3141 0.31 -0.1 3,633,588 - Pf6N2E2_3141 0.31 -0.9 3,633,608 + Pf6N2E2_3141 0.33 -1.0 3,633,609 - Pf6N2E2_3141 0.33 +0.5 3,633,816 + Pf6N2E2_3141 0.48 +0.4 3,633,817 - Pf6N2E2_3141 0.48 +0.0 3,633,817 - Pf6N2E2_3141 0.48 +0.6 3,633,817 - Pf6N2E2_3141 0.48 -0.7 3,633,851 + Pf6N2E2_3141 0.50 +0.4 3,633,851 + Pf6N2E2_3141 0.50 -0.1 3,633,851 + Pf6N2E2_3141 0.50 +2.2 3,633,852 - Pf6N2E2_3141 0.50 +0.4 3,633,852 - Pf6N2E2_3141 0.50 +0.7 3,633,874 - Pf6N2E2_3141 0.52 +0.8 3,633,909 + Pf6N2E2_3141 0.55 +0.1 3,633,909 + Pf6N2E2_3141 0.55 -1.4 3,633,909 + Pf6N2E2_3141 0.55 +1.2 3,633,910 - Pf6N2E2_3141 0.55 +0.2 3,633,910 - Pf6N2E2_3141 0.55 -0.4 3,633,948 + Pf6N2E2_3141 0.57 -0.0 3,633,948 + Pf6N2E2_3141 0.57 -0.5 3,633,949 - Pf6N2E2_3141 0.57 -1.1 3,633,949 - Pf6N2E2_3141 0.57 +0.3 3,633,949 - Pf6N2E2_3141 0.57 -1.5 3,633,949 - Pf6N2E2_3141 0.57 +0.5 3,633,949 - Pf6N2E2_3141 0.57 -0.0
Or see this region's nucleotide sequence