Experiment: Calcium chloride 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_240 and Pf6N2E2_241 are separated by 482 nucleotides Pf6N2E2_241 and Pf6N2E2_242 overlap by 1 nucleotides Pf6N2E2_242 and Pf6N2E2_243 are separated by 49 nucleotides Pf6N2E2_243 and Pf6N2E2_244 are separated by 36 nucleotides
Pf6N2E2_240 - L-proline glycine betaine ABC transport system permease protein ProV (TC 3.A.1.12.1), at 301,003 to 301,893
_240
Pf6N2E2_241 - hypothetical protein, at 302,376 to 302,519
_241
Pf6N2E2_242 - Glycine betaine ABC transport system permease protein, at 302,519 to 303,202
_242
Pf6N2E2_243 - L-proline glycine betaine binding ABC transporter protein ProX (TC 3.A.1.12.1), at 303,252 to 304,157
_243
Pf6N2E2_244 - Nitrilotriacetate monooxygenase component A (EC 1.14.13.-), at 304,194 to 305,522
_244
Position (kb) 302
303
304 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 301.641 kb on + strand, within Pf6N2E2_240 at 301.642 kb on - strand, within Pf6N2E2_240 at 301.952 kb on + strand at 301.981 kb on + strand at 302.115 kb on - strand at 302.157 kb on + strand at 302.157 kb on + strand at 302.157 kb on + strand at 302.244 kb on + strand at 302.336 kb on - strand at 302.481 kb on + strand, within Pf6N2E2_241 at 302.540 kb on + strand at 302.540 kb on + strand at 302.612 kb on + strand, within Pf6N2E2_242 at 302.677 kb on + strand, within Pf6N2E2_242 at 302.682 kb on - strand, within Pf6N2E2_242 at 302.840 kb on - strand, within Pf6N2E2_242 at 302.843 kb on + strand, within Pf6N2E2_242 at 302.843 kb on + strand, within Pf6N2E2_242 at 302.843 kb on + strand, within Pf6N2E2_242 at 302.843 kb on + strand, within Pf6N2E2_242 at 302.843 kb on + strand, within Pf6N2E2_242 at 302.843 kb on + strand, within Pf6N2E2_242 at 302.843 kb on + strand, within Pf6N2E2_242 at 302.843 kb on + strand, within Pf6N2E2_242 at 302.844 kb on - strand, within Pf6N2E2_242 at 302.844 kb on - strand, within Pf6N2E2_242 at 302.989 kb on + strand, within Pf6N2E2_242 at 302.989 kb on + strand, within Pf6N2E2_242 at 302.989 kb on + strand, within Pf6N2E2_242 at 302.990 kb on - strand, within Pf6N2E2_242 at 303.196 kb on + strand at 303.405 kb on + strand, within Pf6N2E2_243 at 303.405 kb on + strand, within Pf6N2E2_243 at 303.405 kb on + strand, within Pf6N2E2_243 at 303.405 kb on + strand, within Pf6N2E2_243 at 303.406 kb on - strand, within Pf6N2E2_243 at 303.519 kb on + strand, within Pf6N2E2_243 at 303.520 kb on - strand, within Pf6N2E2_243 at 303.520 kb on - strand, within Pf6N2E2_243 at 303.523 kb on + strand, within Pf6N2E2_243 at 303.523 kb on + strand, within Pf6N2E2_243 at 303.523 kb on + strand, within Pf6N2E2_243 at 303.523 kb on + strand, within Pf6N2E2_243 at 303.523 kb on + strand, within Pf6N2E2_243 at 303.523 kb on + strand, within Pf6N2E2_243 at 303.523 kb on + strand, within Pf6N2E2_243 at 303.647 kb on + strand, within Pf6N2E2_243 at 303.648 kb on - strand, within Pf6N2E2_243 at 303.687 kb on + strand, within Pf6N2E2_243 at 303.773 kb on - strand, within Pf6N2E2_243 at 303.799 kb on - strand, within Pf6N2E2_243 at 303.974 kb on + strand, within Pf6N2E2_243 at 304.136 kb on + strand at 304.176 kb on + strand at 304.176 kb on + strand at 304.177 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Calcium chloride 0.0125 M remove 301,641 + Pf6N2E2_240 0.72 -0.2 301,642 - Pf6N2E2_240 0.72 +0.1 301,952 + -0.5 301,981 + -1.8 302,115 - -1.3 302,157 + -0.3 302,157 + -1.0 302,157 + -0.8 302,244 + -1.2 302,336 - -0.7 302,481 + Pf6N2E2_241 0.73 +0.7 302,540 + -0.2 302,540 + -0.8 302,612 + Pf6N2E2_242 0.14 -1.7 302,677 + Pf6N2E2_242 0.23 -0.7 302,682 - Pf6N2E2_242 0.24 -2.4 302,840 - Pf6N2E2_242 0.47 -0.7 302,843 + Pf6N2E2_242 0.47 -0.6 302,843 + Pf6N2E2_242 0.47 -0.1 302,843 + Pf6N2E2_242 0.47 -0.0 302,843 + Pf6N2E2_242 0.47 -2.3 302,843 + Pf6N2E2_242 0.47 +0.3 302,843 + Pf6N2E2_242 0.47 -0.5 302,843 + Pf6N2E2_242 0.47 -0.4 302,843 + Pf6N2E2_242 0.47 +0.1 302,844 - Pf6N2E2_242 0.48 -1.1 302,844 - Pf6N2E2_242 0.48 -1.4 302,989 + Pf6N2E2_242 0.69 -1.9 302,989 + Pf6N2E2_242 0.69 -1.3 302,989 + Pf6N2E2_242 0.69 +0.7 302,990 - Pf6N2E2_242 0.69 -0.3 303,196 + +1.5 303,405 + Pf6N2E2_243 0.17 -0.0 303,405 + Pf6N2E2_243 0.17 -0.7 303,405 + Pf6N2E2_243 0.17 +0.6 303,405 + Pf6N2E2_243 0.17 +0.6 303,406 - Pf6N2E2_243 0.17 -0.5 303,519 + Pf6N2E2_243 0.29 +0.2 303,520 - Pf6N2E2_243 0.30 -0.1 303,520 - Pf6N2E2_243 0.30 +2.6 303,523 + Pf6N2E2_243 0.30 +0.2 303,523 + Pf6N2E2_243 0.30 -0.1 303,523 + Pf6N2E2_243 0.30 -0.7 303,523 + Pf6N2E2_243 0.30 -0.6 303,523 + Pf6N2E2_243 0.30 -0.6 303,523 + Pf6N2E2_243 0.30 -0.4 303,523 + Pf6N2E2_243 0.30 -1.1 303,647 + Pf6N2E2_243 0.44 -0.3 303,648 - Pf6N2E2_243 0.44 +0.4 303,687 + Pf6N2E2_243 0.48 +0.1 303,773 - Pf6N2E2_243 0.58 +3.3 303,799 - Pf6N2E2_243 0.60 -0.3 303,974 + Pf6N2E2_243 0.80 -0.3 304,136 + +1.1 304,176 + +0.0 304,176 + -0.6 304,177 - +0.5
Or see this region's nucleotide sequence