Experiment: Calcium chloride 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2328 and Pf6N2E2_2329 are separated by 858 nucleotides Pf6N2E2_2329 and Pf6N2E2_2330 are separated by 326 nucleotides
Pf6N2E2_2328 - DNA polymerase III subunits gamma and tau (EC 2.7.7.7), at 2,749,575 to 2,751,668
_2328
Pf6N2E2_2329 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 2,752,527 to 2,754,287
_2329
Pf6N2E2_2330 - Transcriptional regulator, Cro/CI family, at 2,754,614 to 2,754,928
_2330
Position (kb)
2752
2753
2754
2755 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2751.695 kb on - strand at 2751.695 kb on - strand at 2751.747 kb on + strand at 2751.748 kb on - strand at 2751.748 kb on - strand at 2751.748 kb on - strand at 2751.748 kb on - strand at 2751.757 kb on - strand at 2751.757 kb on - strand at 2751.810 kb on + strand at 2751.811 kb on - strand at 2751.904 kb on - strand at 2751.904 kb on - strand at 2751.959 kb on + strand at 2751.959 kb on + strand at 2751.959 kb on + strand at 2751.959 kb on + strand at 2751.959 kb on + strand at 2751.959 kb on + strand at 2751.959 kb on + strand at 2751.960 kb on - strand at 2751.960 kb on - strand at 2751.960 kb on - strand at 2751.960 kb on - strand at 2751.960 kb on - strand at 2751.960 kb on - strand at 2751.960 kb on - strand at 2752.058 kb on + strand at 2752.058 kb on + strand at 2752.058 kb on + strand at 2752.059 kb on - strand at 2752.059 kb on - strand at 2752.066 kb on + strand at 2752.066 kb on + strand at 2752.066 kb on + strand at 2752.067 kb on - strand at 2752.067 kb on - strand at 2752.067 kb on - strand at 2752.067 kb on - strand at 2752.102 kb on + strand at 2752.102 kb on + strand at 2752.103 kb on - strand at 2752.103 kb on - strand at 2752.202 kb on + strand at 2752.203 kb on - strand at 2752.203 kb on - strand at 2752.219 kb on + strand at 2752.219 kb on + strand at 2752.219 kb on + strand at 2752.220 kb on - strand at 2752.220 kb on - strand at 2752.220 kb on - strand at 2752.221 kb on + strand at 2752.221 kb on + strand at 2752.221 kb on + strand at 2752.222 kb on - strand at 2752.222 kb on - strand at 2752.225 kb on + strand at 2752.226 kb on - strand at 2752.323 kb on + strand at 2752.323 kb on + strand at 2752.323 kb on + strand at 2752.323 kb on + strand at 2752.323 kb on + strand at 2752.323 kb on + strand at 2752.323 kb on + strand at 2752.323 kb on + strand at 2752.323 kb on + strand at 2752.323 kb on + strand at 2752.323 kb on + strand at 2752.323 kb on + strand at 2752.324 kb on - strand at 2752.324 kb on - strand at 2752.324 kb on - strand at 2752.324 kb on - strand at 2752.324 kb on - strand at 2752.324 kb on - strand at 2752.324 kb on - strand at 2752.324 kb on - strand at 2752.324 kb on - strand at 2752.324 kb on - strand at 2752.324 kb on - strand at 2752.324 kb on - strand at 2752.324 kb on - strand at 2752.324 kb on - strand at 2752.324 kb on - strand at 2752.324 kb on - strand at 2752.327 kb on + strand at 2752.327 kb on + strand at 2752.327 kb on + strand at 2752.327 kb on + strand at 2752.328 kb on - strand at 2752.328 kb on - strand at 2752.328 kb on - strand at 2752.328 kb on - strand at 2752.328 kb on - strand at 2752.432 kb on + strand at 2752.432 kb on + strand at 2752.432 kb on + strand at 2752.432 kb on + strand at 2752.528 kb on + strand at 2752.529 kb on - strand at 2752.529 kb on - strand at 2752.529 kb on - strand at 2752.529 kb on - strand at 2752.672 kb on + strand at 2752.673 kb on - strand at 2752.673 kb on - strand at 2753.023 kb on - strand, within Pf6N2E2_2329 at 2753.096 kb on + strand, within Pf6N2E2_2329 at 2753.097 kb on - strand, within Pf6N2E2_2329 at 2753.097 kb on - strand, within Pf6N2E2_2329 at 2753.155 kb on - strand, within Pf6N2E2_2329 at 2753.449 kb on + strand, within Pf6N2E2_2329 at 2753.449 kb on + strand, within Pf6N2E2_2329 at 2753.450 kb on - strand, within Pf6N2E2_2329 at 2753.450 kb on - strand, within Pf6N2E2_2329 at 2753.450 kb on - strand, within Pf6N2E2_2329 at 2753.691 kb on - strand, within Pf6N2E2_2329 at 2753.854 kb on + strand, within Pf6N2E2_2329 at 2753.854 kb on + strand, within Pf6N2E2_2329 at 2753.854 kb on + strand, within Pf6N2E2_2329 at 2753.854 kb on + strand, within Pf6N2E2_2329 at 2753.854 kb on + strand, within Pf6N2E2_2329 at 2753.855 kb on - strand, within Pf6N2E2_2329 at 2753.855 kb on - strand, within Pf6N2E2_2329 at 2753.855 kb on - strand, within Pf6N2E2_2329 at 2753.855 kb on - strand, within Pf6N2E2_2329 at 2753.855 kb on - strand, within Pf6N2E2_2329 at 2753.975 kb on + strand, within Pf6N2E2_2329 at 2753.975 kb on + strand, within Pf6N2E2_2329 at 2754.016 kb on + strand, within Pf6N2E2_2329 at 2754.017 kb on - strand, within Pf6N2E2_2329 at 2754.017 kb on - strand, within Pf6N2E2_2329 at 2754.017 kb on - strand, within Pf6N2E2_2329 at 2754.023 kb on + strand, within Pf6N2E2_2329 at 2754.199 kb on + strand at 2754.199 kb on + strand at 2754.199 kb on + strand at 2754.199 kb on + strand at 2754.200 kb on - strand at 2754.200 kb on - strand at 2754.200 kb on - strand at 2754.262 kb on + strand at 2754.350 kb on + strand at 2754.350 kb on + strand at 2754.350 kb on + strand at 2754.398 kb on + strand at 2754.398 kb on + strand at 2754.399 kb on - strand at 2754.491 kb on + strand at 2754.492 kb on - strand at 2754.492 kb on - strand at 2754.539 kb on + strand at 2754.539 kb on + strand at 2754.540 kb on - strand at 2754.540 kb on - strand at 2754.670 kb on + strand, within Pf6N2E2_2330 at 2754.741 kb on - strand, within Pf6N2E2_2330 at 2754.741 kb on - strand, within Pf6N2E2_2330 at 2754.741 kb on - strand, within Pf6N2E2_2330 at 2754.741 kb on - strand, within Pf6N2E2_2330 at 2754.789 kb on + strand, within Pf6N2E2_2330 at 2754.790 kb on - strand, within Pf6N2E2_2330 at 2754.790 kb on - strand, within Pf6N2E2_2330 at 2754.790 kb on - strand, within Pf6N2E2_2330 at 2754.842 kb on + strand, within Pf6N2E2_2330 at 2754.843 kb on - strand, within Pf6N2E2_2330 at 2754.843 kb on - strand, within Pf6N2E2_2330 at 2754.858 kb on + strand, within Pf6N2E2_2330 at 2754.858 kb on + strand, within Pf6N2E2_2330 at 2754.859 kb on - strand, within Pf6N2E2_2330 at 2754.859 kb on - strand, within Pf6N2E2_2330 at 2754.943 kb on - strand at 2755.071 kb on - strand at 2755.095 kb on + strand at 2755.095 kb on + strand at 2755.096 kb on - strand at 2755.105 kb on - strand at 2755.105 kb on - strand at 2755.105 kb on - strand at 2755.122 kb on + strand at 2755.197 kb on + strand at 2755.198 kb on - strand at 2755.198 kb on - strand at 2755.198 kb on - strand at 2755.213 kb on + strand at 2755.213 kb on + strand at 2755.214 kb on - strand at 2755.239 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Calcium chloride 0.0125 M remove 2,751,695 - -0.7 2,751,695 - +0.5 2,751,747 + -0.9 2,751,748 - -1.5 2,751,748 - +0.4 2,751,748 - -0.0 2,751,748 - -0.5 2,751,757 - +1.1 2,751,757 - -0.2 2,751,810 + +1.1 2,751,811 - -0.6 2,751,904 - -0.1 2,751,904 - +1.1 2,751,959 + -1.0 2,751,959 + +0.1 2,751,959 + -0.4 2,751,959 + -0.7 2,751,959 + -0.5 2,751,959 + -0.3 2,751,959 + -0.6 2,751,960 - -0.3 2,751,960 - +1.3 2,751,960 - +1.8 2,751,960 - +0.0 2,751,960 - +0.7 2,751,960 - -0.7 2,751,960 - -0.9 2,752,058 + +0.1 2,752,058 + -0.1 2,752,058 + +0.1 2,752,059 - +0.6 2,752,059 - -0.9 2,752,066 + +0.5 2,752,066 + -1.3 2,752,066 + -0.4 2,752,067 - -0.0 2,752,067 - +0.6 2,752,067 - -0.1 2,752,067 - -0.5 2,752,102 + +2.0 2,752,102 + -0.8 2,752,103 - +0.6 2,752,103 - -0.8 2,752,202 + -1.3 2,752,203 - +0.1 2,752,203 - -0.0 2,752,219 + -0.2 2,752,219 + +0.1 2,752,219 + -0.1 2,752,220 - -0.2 2,752,220 - +1.0 2,752,220 - +0.2 2,752,221 + +0.2 2,752,221 + -0.3 2,752,221 + -1.3 2,752,222 - -0.1 2,752,222 - +0.4 2,752,225 + +0.1 2,752,226 - -1.0 2,752,323 + -0.4 2,752,323 + +0.9 2,752,323 + +0.7 2,752,323 + +0.3 2,752,323 + +0.7 2,752,323 + +0.4 2,752,323 + -1.1 2,752,323 + -0.3 2,752,323 + +0.2 2,752,323 + -0.4 2,752,323 + -1.5 2,752,323 + -1.0 2,752,324 - -0.4 2,752,324 - -0.2 2,752,324 - +0.3 2,752,324 - -0.6 2,752,324 - +0.3 2,752,324 - -0.0 2,752,324 - -0.2 2,752,324 - +0.0 2,752,324 - +0.4 2,752,324 - -0.1 2,752,324 - -0.2 2,752,324 - -0.3 2,752,324 - -1.1 2,752,324 - -0.7 2,752,324 - +0.8 2,752,324 - +1.0 2,752,327 + -0.9 2,752,327 + -1.5 2,752,327 + +0.1 2,752,327 + -1.1 2,752,328 - +0.4 2,752,328 - -0.1 2,752,328 - -0.2 2,752,328 - +0.1 2,752,328 - -0.3 2,752,432 + -1.0 2,752,432 + +0.0 2,752,432 + +0.4 2,752,432 + +0.2 2,752,528 + -0.3 2,752,529 - -0.0 2,752,529 - +0.3 2,752,529 - +0.2 2,752,529 - -0.3 2,752,672 + -1.5 2,752,673 - -0.3 2,752,673 - -0.1 2,753,023 - Pf6N2E2_2329 0.28 -1.4 2,753,096 + Pf6N2E2_2329 0.32 -1.9 2,753,097 - Pf6N2E2_2329 0.32 +0.5 2,753,097 - Pf6N2E2_2329 0.32 -0.7 2,753,155 - Pf6N2E2_2329 0.36 -1.8 2,753,449 + Pf6N2E2_2329 0.52 -0.0 2,753,449 + Pf6N2E2_2329 0.52 -0.5 2,753,450 - Pf6N2E2_2329 0.52 -0.3 2,753,450 - Pf6N2E2_2329 0.52 +0.2 2,753,450 - Pf6N2E2_2329 0.52 -2.3 2,753,691 - Pf6N2E2_2329 0.66 +0.1 2,753,854 + Pf6N2E2_2329 0.75 -1.1 2,753,854 + Pf6N2E2_2329 0.75 -0.6 2,753,854 + Pf6N2E2_2329 0.75 -2.0 2,753,854 + Pf6N2E2_2329 0.75 -0.3 2,753,854 + Pf6N2E2_2329 0.75 +0.3 2,753,855 - Pf6N2E2_2329 0.75 -1.4 2,753,855 - Pf6N2E2_2329 0.75 -0.2 2,753,855 - Pf6N2E2_2329 0.75 -2.1 2,753,855 - Pf6N2E2_2329 0.75 -0.6 2,753,855 - Pf6N2E2_2329 0.75 -0.4 2,753,975 + Pf6N2E2_2329 0.82 +0.7 2,753,975 + Pf6N2E2_2329 0.82 -1.0 2,754,016 + Pf6N2E2_2329 0.85 -0.3 2,754,017 - Pf6N2E2_2329 0.85 -0.2 2,754,017 - Pf6N2E2_2329 0.85 -2.0 2,754,017 - Pf6N2E2_2329 0.85 -0.6 2,754,023 + Pf6N2E2_2329 0.85 -0.5 2,754,199 + -0.8 2,754,199 + -0.5 2,754,199 + +0.7 2,754,199 + +0.1 2,754,200 - -0.3 2,754,200 - -0.3 2,754,200 - -0.8 2,754,262 + +0.4 2,754,350 + +0.9 2,754,350 + -0.0 2,754,350 + +0.1 2,754,398 + -0.5 2,754,398 + +1.3 2,754,399 - +0.3 2,754,491 + +0.3 2,754,492 - -0.8 2,754,492 - +2.5 2,754,539 + -0.0 2,754,539 + +0.5 2,754,540 - -0.5 2,754,540 - +1.3 2,754,670 + Pf6N2E2_2330 0.18 +0.2 2,754,741 - Pf6N2E2_2330 0.40 +0.2 2,754,741 - Pf6N2E2_2330 0.40 -2.3 2,754,741 - Pf6N2E2_2330 0.40 +0.2 2,754,741 - Pf6N2E2_2330 0.40 +0.6 2,754,789 + Pf6N2E2_2330 0.56 -1.7 2,754,790 - Pf6N2E2_2330 0.56 +1.9 2,754,790 - Pf6N2E2_2330 0.56 +0.1 2,754,790 - Pf6N2E2_2330 0.56 -1.7 2,754,842 + Pf6N2E2_2330 0.72 -0.7 2,754,843 - Pf6N2E2_2330 0.73 +1.0 2,754,843 - Pf6N2E2_2330 0.73 +0.7 2,754,858 + Pf6N2E2_2330 0.77 -0.3 2,754,858 + Pf6N2E2_2330 0.77 -1.8 2,754,859 - Pf6N2E2_2330 0.78 +0.2 2,754,859 - Pf6N2E2_2330 0.78 +1.1 2,754,943 - +1.5 2,755,071 - +0.5 2,755,095 + +0.2 2,755,095 + -2.0 2,755,096 - +0.0 2,755,105 - +0.2 2,755,105 - -0.4 2,755,105 - -1.3 2,755,122 + -0.6 2,755,197 + +0.1 2,755,198 - +0.3 2,755,198 - +0.6 2,755,198 - -0.2 2,755,213 + -0.8 2,755,213 + +0.2 2,755,214 - -1.5 2,755,239 - -0.4
Or see this region's nucleotide sequence