Experiment: Calcium chloride 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_215 and Pf6N2E2_216 are separated by 225 nucleotides Pf6N2E2_216 and Pf6N2E2_217 overlap by 4 nucleotides
Pf6N2E2_215 - Outer membrane protein assembly factor YaeT precursor, at 271,909 to 274,275
_215
Pf6N2E2_216 - Copper-sensing two-component system response regulator CpxR, at 274,501 to 275,184
_216
Pf6N2E2_217 - Signal transduction histidine kinase, at 275,181 to 276,443
_217
Position (kb) 274
275
276 Strain fitness (log2 ratio) -2
-1
0
1
2 at 274.310 kb on + strand at 274.310 kb on + strand at 274.310 kb on + strand at 274.310 kb on + strand at 274.310 kb on + strand at 274.311 kb on - strand at 274.311 kb on - strand at 274.311 kb on - strand at 274.311 kb on - strand at 274.380 kb on + strand at 274.422 kb on + strand at 274.423 kb on - strand at 274.426 kb on + strand at 274.485 kb on + strand at 274.485 kb on + strand at 274.511 kb on + strand at 274.511 kb on + strand at 274.512 kb on - strand at 274.512 kb on - strand at 274.638 kb on + strand, within Pf6N2E2_216 at 274.638 kb on + strand, within Pf6N2E2_216 at 274.638 kb on + strand, within Pf6N2E2_216 at 274.639 kb on - strand, within Pf6N2E2_216 at 274.640 kb on - strand, within Pf6N2E2_216 at 274.792 kb on + strand, within Pf6N2E2_216 at 274.792 kb on + strand, within Pf6N2E2_216 at 274.792 kb on + strand, within Pf6N2E2_216 at 274.793 kb on - strand, within Pf6N2E2_216 at 275.066 kb on - strand, within Pf6N2E2_216 at 275.164 kb on + strand at 275.322 kb on - strand, within Pf6N2E2_217 at 275.335 kb on + strand, within Pf6N2E2_217 at 275.335 kb on + strand, within Pf6N2E2_217 at 275.336 kb on - strand, within Pf6N2E2_217 at 275.336 kb on - strand, within Pf6N2E2_217 at 275.415 kb on + strand, within Pf6N2E2_217 at 275.467 kb on - strand, within Pf6N2E2_217 at 275.607 kb on + strand, within Pf6N2E2_217 at 275.607 kb on + strand, within Pf6N2E2_217 at 275.620 kb on - strand, within Pf6N2E2_217 at 275.779 kb on + strand, within Pf6N2E2_217
Per-strain Table
Position Strand Gene LocusTag Fraction Calcium chloride 0.0125 M remove 274,310 + +0.4 274,310 + +0.4 274,310 + -0.3 274,310 + +0.7 274,310 + +1.6 274,311 - -2.3 274,311 - -0.3 274,311 - -0.3 274,311 - -2.5 274,380 + +0.2 274,422 + +0.5 274,423 - -1.9 274,426 + -0.3 274,485 + +0.2 274,485 + +0.3 274,511 + +0.5 274,511 + +1.3 274,512 - +1.8 274,512 - -0.9 274,638 + Pf6N2E2_216 0.20 +0.1 274,638 + Pf6N2E2_216 0.20 -0.7 274,638 + Pf6N2E2_216 0.20 -1.5 274,639 - Pf6N2E2_216 0.20 -0.3 274,640 - Pf6N2E2_216 0.20 -1.0 274,792 + Pf6N2E2_216 0.43 -0.6 274,792 + Pf6N2E2_216 0.43 -2.2 274,792 + Pf6N2E2_216 0.43 -1.0 274,793 - Pf6N2E2_216 0.43 +0.0 275,066 - Pf6N2E2_216 0.83 +0.5 275,164 + -0.2 275,322 - Pf6N2E2_217 0.11 -0.5 275,335 + Pf6N2E2_217 0.12 -0.5 275,335 + Pf6N2E2_217 0.12 -0.5 275,336 - Pf6N2E2_217 0.12 +0.1 275,336 - Pf6N2E2_217 0.12 -1.4 275,415 + Pf6N2E2_217 0.19 +0.6 275,467 - Pf6N2E2_217 0.23 +0.3 275,607 + Pf6N2E2_217 0.34 -0.7 275,607 + Pf6N2E2_217 0.34 -0.5 275,620 - Pf6N2E2_217 0.35 +0.7 275,779 + Pf6N2E2_217 0.47 +0.9
Or see this region's nucleotide sequence