Experiment: Calcium chloride 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1830 and Pf6N2E2_1831 are separated by 17 nucleotides Pf6N2E2_1831 and Pf6N2E2_1832 overlap by 4 nucleotides Pf6N2E2_1832 and Pf6N2E2_1833 overlap by 8 nucleotides
Pf6N2E2_1830 - Methylsuccinyl-CoA dehydrogenase, predicted by (Erb et al, 2007), at 2,167,717 to 2,169,387
_1830
Pf6N2E2_1831 - Citrate lyase beta chain (EC 4.1.3.6), at 2,169,405 to 2,170,304
_1831
Pf6N2E2_1832 - COGs COG3777, at 2,170,301 to 2,171,158
_1832
Pf6N2E2_1833 - 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / Enoyl CoA hydratase (EC 4.2.1.17), at 2,171,151 to 2,172,668
_1833
Position (kb)
2170
2171
2172 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2169.387 kb on + strand at 2169.387 kb on + strand at 2169.387 kb on + strand at 2169.387 kb on + strand at 2169.388 kb on - strand at 2169.388 kb on - strand at 2169.410 kb on + strand at 2169.411 kb on - strand at 2169.411 kb on - strand at 2169.888 kb on + strand, within Pf6N2E2_1831 at 2169.904 kb on - strand, within Pf6N2E2_1831 at 2169.904 kb on - strand, within Pf6N2E2_1831 at 2170.088 kb on + strand, within Pf6N2E2_1831 at 2170.088 kb on + strand, within Pf6N2E2_1831 at 2170.088 kb on + strand, within Pf6N2E2_1831 at 2170.104 kb on + strand, within Pf6N2E2_1831 at 2170.104 kb on + strand, within Pf6N2E2_1831 at 2170.105 kb on - strand, within Pf6N2E2_1831 at 2170.105 kb on - strand, within Pf6N2E2_1831 at 2170.253 kb on + strand at 2170.406 kb on + strand, within Pf6N2E2_1832 at 2170.407 kb on - strand, within Pf6N2E2_1832 at 2170.407 kb on - strand, within Pf6N2E2_1832 at 2170.407 kb on - strand, within Pf6N2E2_1832 at 2170.463 kb on + strand, within Pf6N2E2_1832 at 2170.500 kb on - strand, within Pf6N2E2_1832 at 2170.572 kb on + strand, within Pf6N2E2_1832 at 2170.649 kb on - strand, within Pf6N2E2_1832 at 2170.903 kb on + strand, within Pf6N2E2_1832 at 2170.903 kb on + strand, within Pf6N2E2_1832 at 2170.903 kb on + strand, within Pf6N2E2_1832 at 2170.903 kb on + strand, within Pf6N2E2_1832 at 2170.903 kb on + strand, within Pf6N2E2_1832 at 2170.903 kb on + strand, within Pf6N2E2_1832 at 2170.903 kb on + strand, within Pf6N2E2_1832 at 2170.904 kb on - strand, within Pf6N2E2_1832 at 2170.904 kb on - strand, within Pf6N2E2_1832 at 2170.996 kb on - strand, within Pf6N2E2_1832 at 2171.036 kb on - strand, within Pf6N2E2_1832 at 2171.036 kb on - strand, within Pf6N2E2_1832 at 2171.136 kb on + strand at 2171.136 kb on + strand at 2171.136 kb on + strand at 2171.137 kb on - strand at 2171.181 kb on + strand at 2171.655 kb on + strand, within Pf6N2E2_1833 at 2171.655 kb on + strand, within Pf6N2E2_1833 at 2171.657 kb on + strand, within Pf6N2E2_1833 at 2171.926 kb on + strand, within Pf6N2E2_1833 at 2171.926 kb on + strand, within Pf6N2E2_1833 at 2172.079 kb on + strand, within Pf6N2E2_1833 at 2172.081 kb on + strand, within Pf6N2E2_1833 at 2172.156 kb on + strand, within Pf6N2E2_1833 at 2172.156 kb on + strand, within Pf6N2E2_1833
Per-strain Table
Position Strand Gene LocusTag Fraction Calcium chloride 0.0125 M remove 2,169,387 + -3.0 2,169,387 + -0.4 2,169,387 + -0.8 2,169,387 + -0.4 2,169,388 - +0.7 2,169,388 - +0.1 2,169,410 + -3.0 2,169,411 - -0.9 2,169,411 - +0.9 2,169,888 + Pf6N2E2_1831 0.54 +0.7 2,169,904 - Pf6N2E2_1831 0.55 -0.3 2,169,904 - Pf6N2E2_1831 0.55 +0.5 2,170,088 + Pf6N2E2_1831 0.76 +0.8 2,170,088 + Pf6N2E2_1831 0.76 +0.0 2,170,088 + Pf6N2E2_1831 0.76 +0.4 2,170,104 + Pf6N2E2_1831 0.78 -0.1 2,170,104 + Pf6N2E2_1831 0.78 +0.5 2,170,105 - Pf6N2E2_1831 0.78 -0.4 2,170,105 - Pf6N2E2_1831 0.78 -1.7 2,170,253 + +2.9 2,170,406 + Pf6N2E2_1832 0.12 -0.2 2,170,407 - Pf6N2E2_1832 0.12 -0.8 2,170,407 - Pf6N2E2_1832 0.12 -0.7 2,170,407 - Pf6N2E2_1832 0.12 -0.3 2,170,463 + Pf6N2E2_1832 0.19 -1.0 2,170,500 - Pf6N2E2_1832 0.23 -1.2 2,170,572 + Pf6N2E2_1832 0.32 -0.5 2,170,649 - Pf6N2E2_1832 0.41 -0.4 2,170,903 + Pf6N2E2_1832 0.70 +0.6 2,170,903 + Pf6N2E2_1832 0.70 +0.0 2,170,903 + Pf6N2E2_1832 0.70 -0.1 2,170,903 + Pf6N2E2_1832 0.70 -1.7 2,170,903 + Pf6N2E2_1832 0.70 -0.6 2,170,903 + Pf6N2E2_1832 0.70 -0.3 2,170,903 + Pf6N2E2_1832 0.70 -0.5 2,170,904 - Pf6N2E2_1832 0.70 +0.5 2,170,904 - Pf6N2E2_1832 0.70 -0.7 2,170,996 - Pf6N2E2_1832 0.81 -1.9 2,171,036 - Pf6N2E2_1832 0.86 -0.7 2,171,036 - Pf6N2E2_1832 0.86 -0.4 2,171,136 + +0.2 2,171,136 + -0.2 2,171,136 + -0.4 2,171,137 - -0.1 2,171,181 + +0.6 2,171,655 + Pf6N2E2_1833 0.33 +1.2 2,171,655 + Pf6N2E2_1833 0.33 +0.4 2,171,657 + Pf6N2E2_1833 0.33 -0.2 2,171,926 + Pf6N2E2_1833 0.51 -0.8 2,171,926 + Pf6N2E2_1833 0.51 +0.1 2,172,079 + Pf6N2E2_1833 0.61 -0.5 2,172,081 + Pf6N2E2_1833 0.61 -1.3 2,172,156 + Pf6N2E2_1833 0.66 -0.7 2,172,156 + Pf6N2E2_1833 0.66 +0.8
Or see this region's nucleotide sequence