Experiment: Calcium chloride 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1583 and Pf6N2E2_1584 are separated by 117 nucleotides Pf6N2E2_1584 and Pf6N2E2_1585 are separated by 83 nucleotides Pf6N2E2_1585 and Pf6N2E2_1586 overlap by 6 nucleotides
Pf6N2E2_1583 - ABC-type protease/lipase transport system, ATPase and permease components, at 1,852,673 to 1,854,457
_1583
Pf6N2E2_1584 - Transcriptional regulator, MarR family / Acetyltransferase (GNAT), at 1,854,575 to 1,855,486
_1584
Pf6N2E2_1585 - hypothetical protein, at 1,855,570 to 1,855,704
_1585
Pf6N2E2_1586 - Transcriptional regulator, TetR family, at 1,855,699 to 1,856,241
_1586
Position (kb)
1854
1855
1856 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1853.676 kb on + strand, within Pf6N2E2_1583 at 1853.723 kb on - strand, within Pf6N2E2_1583 at 1853.875 kb on - strand, within Pf6N2E2_1583 at 1854.024 kb on + strand, within Pf6N2E2_1583 at 1854.024 kb on + strand, within Pf6N2E2_1583 at 1854.025 kb on - strand, within Pf6N2E2_1583 at 1854.163 kb on + strand, within Pf6N2E2_1583 at 1854.163 kb on + strand, within Pf6N2E2_1583 at 1854.163 kb on + strand, within Pf6N2E2_1583 at 1854.165 kb on + strand, within Pf6N2E2_1583 at 1854.166 kb on - strand, within Pf6N2E2_1583 at 1854.245 kb on + strand, within Pf6N2E2_1583 at 1854.295 kb on + strand at 1854.339 kb on + strand at 1854.340 kb on - strand at 1854.351 kb on - strand at 1854.495 kb on - strand at 1854.519 kb on - strand at 1854.776 kb on + strand, within Pf6N2E2_1584 at 1854.882 kb on - strand, within Pf6N2E2_1584 at 1855.006 kb on - strand, within Pf6N2E2_1584 at 1855.129 kb on - strand, within Pf6N2E2_1584 at 1855.172 kb on + strand, within Pf6N2E2_1584 at 1855.173 kb on - strand, within Pf6N2E2_1584 at 1855.412 kb on + strand at 1855.413 kb on - strand at 1855.567 kb on + strand at 1855.568 kb on - strand at 1855.680 kb on + strand, within Pf6N2E2_1585 at 1855.701 kb on - strand at 1855.701 kb on - strand at 1855.701 kb on - strand at 1855.701 kb on - strand at 1855.701 kb on - strand at 1855.778 kb on - strand, within Pf6N2E2_1586 at 1856.324 kb on - strand at 1856.324 kb on - strand at 1856.324 kb on - strand at 1856.327 kb on + strand at 1856.328 kb on - strand at 1856.328 kb on - strand at 1856.387 kb on + strand at 1856.387 kb on + strand at 1856.388 kb on - strand at 1856.388 kb on - strand at 1856.393 kb on - strand at 1856.416 kb on - strand at 1856.416 kb on - strand at 1856.443 kb on + strand at 1856.444 kb on - strand at 1856.444 kb on - strand at 1856.444 kb on - strand at 1856.454 kb on + strand at 1856.455 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Calcium chloride 0.0125 M remove 1,853,676 + Pf6N2E2_1583 0.56 -0.5 1,853,723 - Pf6N2E2_1583 0.59 -0.6 1,853,875 - Pf6N2E2_1583 0.67 -0.2 1,854,024 + Pf6N2E2_1583 0.76 -2.2 1,854,024 + Pf6N2E2_1583 0.76 +0.5 1,854,025 - Pf6N2E2_1583 0.76 -1.2 1,854,163 + Pf6N2E2_1583 0.83 +1.1 1,854,163 + Pf6N2E2_1583 0.83 +0.4 1,854,163 + Pf6N2E2_1583 0.83 +0.2 1,854,165 + Pf6N2E2_1583 0.84 -1.0 1,854,166 - Pf6N2E2_1583 0.84 -0.8 1,854,245 + Pf6N2E2_1583 0.88 +0.3 1,854,295 + +1.5 1,854,339 + -0.8 1,854,340 - +1.4 1,854,351 - +1.1 1,854,495 - +1.4 1,854,519 - -2.9 1,854,776 + Pf6N2E2_1584 0.22 -1.0 1,854,882 - Pf6N2E2_1584 0.34 -0.9 1,855,006 - Pf6N2E2_1584 0.47 -1.7 1,855,129 - Pf6N2E2_1584 0.61 -0.9 1,855,172 + Pf6N2E2_1584 0.65 -1.6 1,855,173 - Pf6N2E2_1584 0.66 -0.1 1,855,412 + +1.2 1,855,413 - -1.0 1,855,567 + -0.6 1,855,568 - +1.5 1,855,680 + Pf6N2E2_1585 0.81 -1.1 1,855,701 - -1.6 1,855,701 - +1.0 1,855,701 - +0.8 1,855,701 - +0.0 1,855,701 - -1.0 1,855,778 - Pf6N2E2_1586 0.15 -1.8 1,856,324 - -0.2 1,856,324 - +0.7 1,856,324 - +0.5 1,856,327 + -0.5 1,856,328 - +0.1 1,856,328 - -1.6 1,856,387 + -0.2 1,856,387 + -1.8 1,856,388 - +0.8 1,856,388 - -1.0 1,856,393 - +0.3 1,856,416 - -0.2 1,856,416 - -0.3 1,856,443 + -0.0 1,856,444 - +1.4 1,856,444 - -0.2 1,856,444 - +1.3 1,856,454 + -0.0 1,856,455 - -0.6
Or see this region's nucleotide sequence